FastQCFastQC Report
Fri 17 Jun 2016
SRR1526107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC392.4466750313676284No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT362.258469259723965No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.5646173149309912No Hit
AGATGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA80.5018820577164366No Hit
AGATGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGC70.43914680050188204No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGAGGGTGAGAGT60.37641154328732745No Hit
AGATGGGGGACTTGTAATTCTTTTCTTGGTCATGTGTGAG40.2509410288582183No Hit
AGATGGGGGGGCTTGAGTGTTCTGCCAGTTCTGGATAATC40.2509410288582183No Hit
AGATGGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT40.2509410288582183No Hit
AGATGGGGGGACACTTGGGTCATGTTCAGTTCCTTACAGT40.2509410288582183No Hit
AGATGGAGGGGGGGAGAGTGCAGAGCTGGCAGGTCAGTTA40.2509410288582183No Hit
AGATGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG30.18820577164366373No Hit
AGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTT30.18820577164366373No Hit
AGATGGGGTTTTTCCCATCTATTATTCACCGCATTACACC30.18820577164366373No Hit
AGATGGGGGGTCTCCCAACATGTTTTCTTGTTCTCTGACC30.18820577164366373No Hit
AGATGGGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGAG30.18820577164366373No Hit
AGATGGGGGGTTGGTTGGTTTGCCCTCTTTTGTTTGCTTC30.18820577164366373No Hit
AGATGGGGGAAGCAGTAGTATCAACCCAGAGTCGACTTTT30.18820577164366373No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC20.12547051442910914No Hit
AGATGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCAC20.12547051442910914No Hit
AGATGGGGGGGTCTCTTTTCTAAAAAGTGTGGGAAAGTGT20.12547051442910914No Hit
AGATGGGGGGGTCCAGCGCGGAAAAGACAGCAAGCAATAT20.12547051442910914No Hit
AGATGGGGTCTTTCACCTTGTTTGAAACAGGATCTCTCTG20.12547051442910914No Hit
AGATGGGGGGAAGTCTGGCCTCACTGCACATCCTGGGGTG20.12547051442910914No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC20.12547051442910914No Hit
AGATGGGGGGTTGGAGAGATGGCTCAGCGGTTAGGAGCAC20.12547051442910914No Hit
AGATGGGGGGTGGAAGGGCCTACTGTTAAAGCCCTGAGTG20.12547051442910914No Hit
AGATGGGGGGGGCTGGAGAGATGGCTTAGCGGTTAAGAGC20.12547051442910914No Hit
AGATGGGGGATGCCAAGTAAATTGTGCAGGTGGATGGGAA20.12547051442910914No Hit
AGATGGGGGGTTTGTTTTCTTTCTTTCTTTTTTGAAATGT20.12547051442910914No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAAAGC20.12547051442910914No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAG20.12547051442910914No Hit
AGATGGGGAATGTTCTATCAATGGGAGCAGTGTTTGCTAT20.12547051442910914No Hit
AGATGGGGGGCTGGAGAGATGGCTGGCTCAGAGGTTAAGA20.12547051442910914No Hit
AGATGGGGGATGCATGTCTAAGTACGCACGGCCGGTACAG20.12547051442910914No Hit
AGATGGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGAT20.12547051442910914No Hit
AGATGGGGGGGGCTGGAGAGATGGCTCTGTGGTTAAGTGC20.12547051442910914No Hit
AGATGGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC20.12547051442910914No Hit
AGATGGGGGGGGGTTGGTGAGATGGCTCAGTGGGTAGGAG20.12547051442910914No Hit
AGATGGGGGGTTGTCAACTTGGCAGAATTCATAATCACTT20.12547051442910914No Hit
AGATGGGGGGCATTGAGCTCTGTGTCTGCTTTATATACGT20.12547051442910914No Hit
AGATGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT20.12547051442910914No Hit
AGATGGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT20.12547051442910914No Hit
AGATGGGGGTGTTTTGTTTTTGTTTTTGTTGTGTTTTTAA20.12547051442910914No Hit
AGATGGGGGGAGTGTACATGAGTATTCCGTGTGCATATGT20.12547051442910914No Hit
AGATGGGGGGCCGCCTGGGTACCGCAGCTAGGAATAATGG20.12547051442910914No Hit
AGATGGGGGGACTGACTGGAGGGGACAGGCTTTGTTTAGG20.12547051442910914No Hit
AGATGGGGGCAACATATCTTATTGACCCAGATATATTTTG20.12547051442910914No Hit
AGATGGGGGAGTGAAAATGCGAATGGCTCATTAAATCAGT20.12547051442910914No Hit
AGATGGGGGGAAGAGCTGTGTAGCTTCAGGAAGCAGAGCC20.12547051442910914No Hit
AGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.12547051442910914No Hit
AGATGGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTG20.12547051442910914No Hit
AGATGGGGGAGTAGAGACGTCTCCTGGCTCTCTCTCTCTC20.12547051442910914No Hit
AGATGGGGGCGGGGAGGTAGTGACGAAAAATAACAATACA20.12547051442910914No Hit
AGATGGGGGGAGAGCTATGTGGCTTTATGTTTATCAAGAG20.12547051442910914No Hit
AGATGGGGGGGGCGTGGTGGCTCCTGCCTTTAATCCCAGC20.12547051442910914No Hit
AGATGGGGGGTACCTGAAGTTTCATCTTCTTATCTTGTGT20.12547051442910914No Hit
AGATGGGGGATGTGTGTTGTGTGTGTGTTTGTGTAGGGAG20.12547051442910914No Hit
AGATGGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA20.12547051442910914No Hit
AGATGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTC20.12547051442910914No Hit
AGATGGGGGAAGTAAAGGCAGAAAAAATAATGTCATGTCT20.12547051442910914No Hit
AGATGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAG20.12547051442910914No Hit
AGATGGGGGGGGGCTGGTGAAATAGCTCAGTGGGTAAGAG20.12547051442910914No Hit
AGATGGGGGGTTTGGAATACTTTGGTTATCCATTCATATT20.12547051442910914No Hit
AGATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCA20.12547051442910914No Hit
AGATGGGGGGCTGTGTTGTGGTAGGAGGTTCCAGATCACA20.12547051442910914No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGA20.12547051442910914No Hit
AGATGGGGGATAGTCTTCACTGCTTCACCTTGTTAAGTCA20.12547051442910914No Hit
AGATGGGGGAAGACTGCTCCCTTCACTTCCTGCTTCGTGT20.12547051442910914No Hit
AGATGGGGGGCACTCCAGTCCATAGCTTGCAGCAGCCAGG20.12547051442910914No Hit
AGATGGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG20.12547051442910914No Hit
AGATGGGGGCTTACTGTTTATTTTAGAGATGGGTGAACAT20.12547051442910914No Hit
AGATGGGGGGAGGAATTCAGGCAGCGAGAGCAGAGCAGCG20.12547051442910914No Hit
AGATGGGGGGTTTTGTAAGTGGATCCTCTTTCCTTGAAAT20.12547051442910914No Hit
AGATGGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT20.12547051442910914No Hit
AGATGGGGGGGGCTGGTGTGATGGCTCAGTGGTTAAGAGC20.12547051442910914No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12547051442910914No Hit
AGATGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCT20.12547051442910914No Hit
AGATGGGGGGTGGGAAATACTCTTTCTGTATATCATTTGG20.12547051442910914No Hit
AGATGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTAT20.12547051442910914No Hit
AGATGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC20.12547051442910914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGG1450.034.02
ATGGGGG1300.034.03
TGGGGGG850.034.04
TGGGGGA251.2417891E-434.04
AGATGGG1450.034.01
GGGGGGC450.003892835218.888897
GGGGGGG1101.0930307E-817.0000025
Produced by FastQC (version 0.11.5)