FastQCFastQC Report
Fri 17 Jun 2016
SRR1526106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65712
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG17522.66617969320672No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT5280.8035062089116143No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG4220.6421962503043585No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC4210.6406744582420257No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2090.318054541027514No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1900.2891404918431945No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1750.2663136109082055No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1540.23435597759922083No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1460.22218164110056005No Hit
GTGGTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1350.2054419284149014No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1340.20392013635256878No Hit
GTGGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1000.15217920623326028No Hit
GTGGTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC970.14761383004626247No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT930.14152666179693207No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA920.14000486973459947No Hit
GTGGTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT900.13696128560993426No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC710.10804723642561481No Hit
GTGGTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA680.10348186023861698No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC670.10196006817628439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAC309.482592E-750.62044541
TTCTGAC150.00799581350.62044541
CGCATTG451.200533E-1050.50309439
AGCTGTC150.00806959450.5030938
GTTCCCT150.00806959450.5030939
GTCCAAT251.946731E-550.5030939
TGCCTGT251.9826693E-550.34745437
TATGCCT309.949072E-750.26999735
TGGGCCG204.0456597E-450.26999735
GCTTAGG150.00824371950.23135814
ATTGACC252.0099824E-550.23135842
CTTAGGT150.00824371950.23135815
CGACTCC150.00826882150.19277644
CCCGATC150.00826882150.19277649
TTGACCA150.00829397950.1542643
GCCCGAT150.00829397950.1542648
TACGCTT150.00834446650.07739311
ACGCTTA150.00834446650.07739312
CCCATCC150.00834446650.07739313
CGCTTAG150.00834446650.07739313
Produced by FastQC (version 0.11.5)