##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526106_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65712 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.8920288531775 38.0 33.0 38.0 29.0 39.0 2 33.77730094959825 38.0 32.0 38.0 24.0 39.0 3 34.52985756026297 38.0 33.0 38.0 27.0 39.0 4 34.304875821767716 38.0 33.0 38.0 25.0 39.0 5 32.303825785244705 35.0 30.0 38.0 20.0 39.0 6 32.134998173849525 38.0 28.0 39.0 15.0 40.0 7 34.81505661066472 38.0 34.0 39.0 27.0 40.0 8 34.735892987582176 38.0 34.0 39.0 27.0 40.0 9 34.65039870952033 38.0 34.0 39.0 27.0 40.0 10 33.81823715607499 38.0 33.0 39.0 23.0 40.0 11 33.73937789140492 38.0 33.0 39.0 23.0 40.0 12 32.59184015096177 36.0 30.0 39.0 19.0 40.0 13 31.909666423179935 36.0 29.0 39.0 16.0 40.0 14 32.700937423910396 37.0 30.0 39.0 19.0 40.0 15 31.52232468955442 36.0 28.0 39.0 15.0 39.0 16 32.18508035062089 36.0 29.0 38.0 18.0 39.0 17 31.17591916240565 35.0 27.0 38.0 15.0 39.0 18 30.602447041636232 35.0 26.0 38.0 13.0 39.0 19 31.6720385926467 35.0 29.0 38.0 17.0 39.0 20 32.271015948380814 36.0 29.0 38.0 20.0 39.0 21 32.601594838081326 36.0 30.0 39.0 19.0 40.0 22 31.884845994643292 36.0 29.0 38.0 16.0 39.0 23 32.617238860482104 36.0 30.0 39.0 19.0 40.0 24 31.945337229121012 36.0 29.0 38.0 17.0 39.0 25 32.33986182128074 36.0 30.0 38.0 19.0 39.0 26 32.62475651327003 37.0 31.0 39.0 18.0 39.0 27 32.91446006817628 37.0 32.0 39.0 19.0 39.0 28 32.854242756269784 37.0 32.0 38.0 20.0 39.0 29 32.39059836863891 36.0 31.0 38.0 17.0 39.0 30 32.07759617725834 36.0 30.0 38.0 17.0 39.0 31 32.663531775018264 36.0 32.0 38.0 18.0 39.0 32 32.6449659118578 36.0 32.0 38.0 18.0 39.0 33 32.37605003652301 36.0 31.0 38.0 18.0 39.0 34 32.098718651083516 36.0 31.0 38.0 16.0 39.0 35 32.13603299245191 36.0 31.0 38.0 15.0 39.0 36 31.52077246165084 36.0 30.0 38.0 7.0 39.0 37 31.26801801801802 35.0 30.0 38.0 4.0 39.0 38 30.60011869978086 35.0 29.0 38.0 4.0 39.0 39 29.382532870708545 35.0 26.0 38.0 4.0 39.0 40 27.40776418310202 33.0 18.0 38.0 4.0 39.0 41 25.502039201363527 31.0 13.0 37.0 4.0 39.0 42 26.102690528366203 31.0 17.0 36.0 4.0 39.0 43 26.384085098612125 32.0 18.0 37.0 4.0 39.0 44 25.437926101777453 31.0 13.0 36.0 4.0 39.0 45 26.141587533479424 32.0 17.0 37.0 4.0 39.0 46 24.11150170440711 29.0 10.0 35.0 4.0 39.0 47 23.362688702215728 28.0 6.0 35.0 4.0 38.0 48 23.927319211102994 29.0 6.0 35.0 4.0 38.0 49 22.938078280983685 27.0 4.0 35.0 4.0 38.0 50 23.47134465546628 28.0 4.0 35.0 4.0 38.0 51 23.762417823228635 30.0 4.0 35.0 4.0 38.0 52 23.568434989043098 29.0 4.0 35.0 4.0 38.0 53 23.405085829072316 29.0 4.0 35.0 4.0 38.0 54 23.00543279766253 29.0 4.0 35.0 4.0 38.0 55 22.710098612125638 29.0 4.0 35.0 4.0 38.0 56 21.81418918918919 27.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 5.362573035590955 1 2 5.856654842413732 1 3 5.693554317905921 1 4 5.882506241361803 1 5 6.124071174156384 1 6 9.137773620870608 1 7 5.756038532756598 1 8 6.019761820591704 1 9 6.136297726754336 1 10 6.197362552444101 1 11 0.7162058497937593 1 12 -7.528374937967136 1 13 -18.253979330374406 1 14 -6.628841200323592 1 15 0.8635290733304188 1 16 -2.4210826423838228 1 17 -5.881059895358916 1 18 -5.469005534746845 1 19 -1.6040137532328131 1 20 -2.218230093279324 1 21 5.896388080643291 1 22 7.215563410773974 1 23 6.370111353027795 1 24 7.065762503942064 1 25 5.896484418224631 1 26 7.163154590177456 1 27 6.461998721224724 1 28 6.151414112961859 1 29 6.705431935230205 1 30 6.15641602556963 1 31 5.315114901383531 1 32 4.650404912867618 1 33 5.005763872780669 1 34 5.201978994843973 1 35 5.131635023616038 1 36 3.3757798828846255 1 37 3.852831692721601 1 38 3.2266133409608173 1 39 3.5970847719911845 1 40 -11.556433622212714 1 41 -2.267830709007562 1 42 -3.327318795222091 1 43 -11.173575752144442 1 44 -14.504413303536607 1 45 -3.373743672981014 1 46 -5.597924714080431 1 47 -10.036245233654057 1 48 -5.298727912163997 1 49 -13.820548370365788 1 50 -4.379541304790482 1 51 1.1264164044424376 1 52 1.6734989719316538 1 53 1.409786142039927 1 54 1.8909039326536927 1 55 1.7495226855348278 1 56 5.596911756732055 2 1 5.233338867696993 2 2 6.03817177908531 2 3 5.484607337051727 2 4 5.620724208961214 2 5 6.2281764760709635 2 6 8.832637391120972 2 7 5.690500977528316 2 8 5.760465060650617 2 9 5.993808772409707 2 10 6.006456808703309 2 11 4.737192595007308 2 12 0.593035224035809 2 13 1.5139698596108673 2 14 5.688997533107923 2 15 6.444254404700079 2 16 4.026818683094824 2 17 6.27891801333033 2 18 3.471347926225171 2 19 5.299152667974848 2 20 4.522012911138937 2 21 6.276082484177898 2 22 6.825007446120068 2 23 5.441631971584499 2 24 6.516609337520858 2 25 6.127246568445543 2 26 5.093290819927091 2 27 5.337366909737241 2 28 5.062036351548009 2 29 5.4094083711654015 2 30 6.091706894494518 2 31 4.290906506685445 2 32 5.323913749391917 2 33 4.36318287130792 2 34 3.73732509204574 2 35 4.0359060103612485 2 36 4.856634080233665 2 37 5.0361895719557666 2 38 5.424698760695719 2 39 6.0335722535817595 2 40 3.84562823345739 2 41 8.50435264887167 2 42 7.541422000065097 2 43 5.891317473186927 2 44 -3.3851202254806445 2 45 7.067070023631651 2 46 5.737310926567581 2 47 2.647941069733278 2 48 5.00484351640743 2 49 -1.1720763526927414 2 50 5.323422612735293 2 51 6.829564416224471 2 52 6.471823714199694 2 53 7.731766996237276 2 54 7.374979779487273 2 55 7.632898974194621 2 56 8.121856528455176 3 1 5.431858816216945 3 2 5.415584906498438 3 3 5.547831275275662 3 4 5.682822021059025 3 5 5.755444815839304 3 6 9.066630312613892 3 7 5.468493255520599 3 8 5.510706373391926 3 9 5.726434255035194 3 10 5.463824891071386 3 11 5.20984381765853 3 12 5.632208325708909 3 13 5.961122369263375 3 14 5.612823144433534 3 15 0.6878998358455135 3 16 5.570094101370238 3 17 6.794522903935221 3 18 7.433381388258631 3 19 6.253142371964547 3 20 5.81882758295361 3 21 6.226935122530541 3 22 7.584096892709518 3 23 6.4861139535664805 3 24 6.805058500970016 3 25 6.651940905639876 3 26 5.621685285821556 3 27 4.66474786211819 3 28 4.7651412421529 3 29 5.78521274696978 3 30 5.597578837866461 3 31 3.536160689439626 3 32 3.2252168381950064 3 33 4.0835014041264515 3 34 4.134364989085636 3 35 4.561002535457778 3 36 5.931923655523246 3 37 6.047129082895275 3 38 6.584930420927378 3 39 7.248984093993599 3 40 8.575357963187116 3 41 11.571678903697926 3 42 10.216936800579898 3 43 10.752401771771225 3 44 11.06919122883081 3 45 11.288836432898062 3 46 11.595258159514817 3 47 11.904778264070469 3 48 11.782353168917084 3 49 13.201556208439818 3 50 11.919706396519075 3 51 11.60361525277531 3 52 11.538828218704197 3 53 11.772468411938693 3 54 10.939571156578648 3 55 12.485939514735161 3 56 10.673256142354791 4 1 5.2684210421597015 4 2 5.800181892865332 4 3 5.404803291539714 4 4 5.568575030705837 4 5 5.1084704078029475 4 6 8.93021956558368 4 7 5.528048385872189 4 8 5.758015629550741 4 9 5.362645687706802 4 10 5.893466922483331 4 11 5.830429004615397 4 12 7.0147324427463005 4 13 7.172076686412385 4 14 6.560283879504894 4 15 7.532354910389085 4 16 7.254220705850827 4 17 7.73704064291314 4 18 9.013067268829474 4 19 6.73372915343629 4 20 6.664079914931605 4 21 7.3066608659731 4 22 7.397937281771142 4 23 6.6280257768942334 4 24 7.2686636990884885 4 25 6.178210538104203 4 26 5.69814227378297 4 27 5.449408629079848 4 28 5.123588184670645 4 29 6.075653630331015 4 30 5.798328259854824 4 31 4.766807265218951 4 32 4.8544921311871185 4 33 5.345878825795907 4 34 4.789790325926905 4 35 5.139572255620418 4 36 5.729579718666034 4 37 5.538718016962086 4 38 6.196506598875239 4 39 6.742326994416146 4 40 2.022619383899869 4 41 11.031138363157385 4 42 9.86016409867445 4 43 9.939278914400582 4 44 11.142060558337306 4 45 10.105865851697391 4 46 12.398973379159234 4 47 12.944322232817978 4 48 12.117201291375828 4 49 12.66444387486731 4 50 12.312913763177768 4 51 11.08691270952409 4 52 11.742288315969603 4 53 12.160812508247393 4 54 11.98852276305238 4 55 12.511770655610551 4 56 11.420924164359096 5 1 5.188365322723449 5 2 5.126796117709645 5 3 5.077861305305696 5 4 5.338978177215182 5 5 5.516205576600065 5 6 8.82238606836965 5 7 5.490765527792867 5 8 5.557753420438978 5 9 5.829787608388543 5 10 5.757118184364682 5 11 5.902536510351219 5 12 6.207783901284486 5 13 6.824485732626737 5 14 6.607317638928031 5 15 7.3440559920016675 5 16 7.130654661930798 5 17 7.806034415446735 5 18 8.537485492362741 5 19 7.295184414006588 5 20 6.27928804341407 5 21 6.675884071479487 5 22 6.871634430247056 5 23 5.430057897510423 5 24 6.730233676145193 5 25 6.450989954688929 5 26 5.432245846382116 5 27 5.322155269525602 5 28 4.906282383294041 5 29 5.352530064287091 5 30 4.430661920949543 5 31 3.595469998748939 5 32 4.83482644780462 5 33 4.120788691413736 5 34 4.480711335431987 5 35 4.731673206128445 5 36 5.545787519387108 5 37 5.895977931744127 5 38 6.064910400907358 5 39 7.409895004904513 5 40 8.540072677901833 5 41 11.262619844638866 5 42 10.587056920700018 5 43 10.301701071070525 5 44 11.362234271873852 5 45 10.154702298763926 5 46 11.421834736091387 5 47 11.907531016823224 5 48 11.395716532280446 5 49 12.539894546778157 5 50 11.69147816829085 5 51 11.761773410933468 5 52 11.506545936421915 5 53 11.29724318671347 5 54 11.584966551974045 5 55 12.380584159379804 5 56 11.018351237449885 6 1 5.405171751794029 6 2 5.966069353209299 6 3 5.579957741364396 6 4 5.775036877424824 6 5 5.578749252351287 6 6 9.255649520501027 6 7 5.635244912838296 6 8 5.700660478440376 6 9 5.983700955698119 6 10 5.276335514902765 6 11 6.140831409023477 6 12 6.414632259076242 6 13 7.16306015610682 6 14 6.748128260870729 6 15 6.8901775852930705 6 16 6.4213604620705595 6 17 6.933567609017661 6 18 8.308275149944848 6 19 6.161012506249779 6 20 5.386905094427348 6 21 6.061628306280326 6 22 6.273626044502819 6 23 5.71167914139582 6 24 6.215733326739187 6 25 5.376741177609958 6 26 5.035844728282882 6 27 4.422609497338321 6 28 4.064563305725912 6 29 5.449839638011763 6 30 4.779543821718239 6 31 3.114862032291917 6 32 4.005685986588684 6 33 4.285184219016813 6 34 3.7508021809945298 6 35 4.033210592571489 6 36 5.4900572608266565 6 37 5.784168008613449 6 38 5.705022210830492 6 39 6.217197590508981 6 40 3.04374144100456 6 41 9.634911948063046 6 42 9.615882916507143 6 43 8.726054669952426 6 44 9.790081930853589 6 45 8.422696708267772 6 46 10.344790710934156 6 47 11.346559668116026 6 48 9.106932465194493 6 49 10.977350871026935 6 50 11.060450914622084 6 51 10.221242313798594 6 52 10.468285978350636 6 53 11.572400419417871 6 54 11.007223714797245 6 55 10.92653104966632 6 56 10.97310315918294 7 1 4.377624581982708 7 2 4.785314636228165 7 3 4.386750194194583 7 4 4.777867066104065 7 5 4.62139076178525 7 6 8.269423105406684 7 7 4.463358120385465 7 8 5.253679346364905 7 9 5.142380200981137 7 10 4.678599665846161 7 11 4.492906880721591 7 12 5.914820938321526 7 13 6.090041288182295 7 14 5.6143546759650675 7 15 6.089611547557222 7 16 5.573247254523395 7 17 6.596775156187473 7 18 7.265633640510885 7 19 6.156295525117702 7 20 5.035584339710368 7 21 5.641439627035041 7 22 5.266078874691502 7 23 4.804131971584496 7 24 5.11875219466371 7 25 4.476175139874115 7 26 3.3915051056413787 7 27 2.978081195451523 7 28 2.7573934944051572 7 29 3.0151226568796865 7 30 1.9999211802088048 7 31 1.7976922209711574 7 32 2.379270892249064 7 33 2.7778257284507717 7 34 1.575896520617171 7 35 2.580191724646962 7 36 3.309491223090813 7 37 4.11190385767005 7 38 3.398984474981436 7 39 4.382857967867476 7 40 5.375628233457391 7 41 7.471138363157387 7 42 7.399279142922239 7 43 8.030960330329783 7 44 8.616308345947928 7 45 6.870998595060225 7 46 9.525168069424723 7 47 9.669012498304706 7 48 8.771272087836003 7 49 9.804709361592973 7 50 8.675922612735292 7 51 7.229921559081614 7 52 8.074323714199693 7 53 7.463909853380134 7 54 7.8053369223444165 7 55 7.9653989741946205 7 56 7.236499385598034 8 1 4.7302332776348806 8 2 4.78661898405425 8 3 4.64066323767284 8 4 4.532214892191021 8 5 4.929651631350467 8 6 8.66377093149364 8 7 4.8877059464724155 8 8 5.222809781147511 8 9 5.270206287937661 8 10 5.119034448454858 8 11 5.251167750286811 8 12 5.712212242669352 8 13 6.130041288182294 8 14 5.970441632486811 8 15 7.421350677992006 8 16 6.505855950175565 8 17 7.992427330100522 8 18 7.7943292926848 8 19 6.764991177291616 8 20 5.86906260057993 8 21 5.264048322687216 8 22 6.957383222517585 8 23 5.232392841149718 8 24 5.41179567292458 8 25 5.636175139874112 8 26 4.213244236076157 8 27 4.237211630234135 8 28 4.7239152335355925 8 29 3.705992222097077 8 30 4.776877701947935 8 31 3.882909612275512 8 32 4.420575240075149 8 33 3.636086598015993 8 34 2.937635651051952 8 35 3.880626507255659 8 36 3.7494912230908106 8 37 4.017556031583098 8 38 4.834636648894481 8 39 5.416336228737038 8 40 0.5956282334573899 8 41 8.878964450113909 8 42 8.146235664661369 8 43 8.310960330329785 8 44 8.577612693774014 8 45 7.429259464625442 8 46 10.222559373772548 8 47 10.530316846130791 8 48 9.136054696531655 8 49 10.311231100723408 8 50 9.809835656213554 8 51 9.094704167777266 8 52 9.403888931590998 8 53 9.289996809901872 8 54 9.607510835387892 8 55 9.77148593071636 8 56 10.242586342119772 9 1 4.534258245349044 9 2 4.986898794644006 9 3 4.8726907882539905 9 4 4.549649244321891 9 5 5.295450167725839 9 6 8.275264689565102 9 7 4.333358120385462 9 8 4.50348132656292 9 9 4.870400002961333 9 10 4.44652045792537 9 11 5.203599950028526 9 12 4.82897935416311 9 13 5.909744258479325 9 14 5.688909131410611 9 15 5.726344220824554 9 16 5.3965145812560635 9 17 6.46261674034589 9 18 6.735138591005935 9 19 5.39639453501869 9 20 4.951821963472746 9 21 5.691835666639008 9 22 4.7442966964736755 9 23 5.256607219109249 9 24 5.013603679812224 9 25 5.004987021062227 9 26 3.976158570987913 9 27 3.8198633736693495 9 28 3.938185573613076 9 29 4.031756320246021 9 30 4.860713259416723 9 31 3.057791230872148 9 32 2.7446174269025256 9 33 3.368221768054738 9 34 2.6955994909142014 9 35 3.5026669721717134 9 36 4.172758549823481 9 37 4.524874154699752 9 38 4.75779635616955 9 39 5.5783035124219325 9 40 3.6971133819722404 9 41 8.159851234444513 9 42 8.539477162724221 9 43 8.251554389735727 9 44 8.99818953406674 9 45 7.5698104762483425 9 46 10.505168069424723 9 47 11.119210518106687 9 48 10.473549315558774 9 49 9.562630153672181 9 50 10.423051325606579 9 51 8.900020568982605 9 52 8.519472229051178 9 53 9.154503912786076 9 54 8.11127751640382 9 55 8.628567291026304 9 56 8.480855821241597 10 1 5.187098266193232 10 2 5.3242620046492135 10 3 5.266750194194579 10 4 5.443656539788279 10 5 5.690864445995771 10 6 8.680475736985638 10 7 4.754410751964407 10 8 5.273153030575429 10 9 5.630274937823245 10 10 5.522810192161952 10 11 5.357117407037379 10 12 6.107978833058368 10 13 6.258988656603346 10 14 6.386459939122965 10 15 7.610137863346697 10 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3.887641083552598 115 25 3.6639529176518906 115 26 2.8998384389747116 115 27 2.4703034176737475 115 28 3.086837938849598 115 29 2.8617893235463505 115 30 2.926032291319917 115 31 3.4565811098600463 115 32 2.8626042255823982 115 33 3.11615906178411 115 34 3.388118742839392 115 35 3.916858391313628 115 36 4.888380111979696 115 37 5.141348302114494 115 38 5.52787336387032 115 39 5.775635745645253 115 40 7.956739344568504 115 41 8.947805029824053 115 42 7.087056920700018 115 43 5.769293663663117 115 44 -1.2581360984965144 115 45 5.353776372838002 115 46 1.1023902916469446 115 47 -8.597098612806406 115 48 0.2151609767248921 115 49 -3.5064017495181368 115 50 1.4275892794019605 115 51 2.312699336859392 115 52 2.4787681586441366 115 53 2.9500209644912445 115 54 2.163670255677747 115 55 2.8481767519723995 115 56 6.152610496709144 116 1 4.412814455400429 116 2 4.601010838759812 116 3 4.289535004321166 116 4 3.7911582053445727 116 5 4.01784645798778 116 6 7.226511713001624 116 7 4.308041664689263 116 8 4.572286941301613 116 9 4.621747289588733 116 10 3.6436629569854055 116 11 3.7792359946456457 116 12 2.6625424573088665 116 13 3.8167501489417894 116 14 4.0304306253321585 116 15 -4.43583149041746 116 16 2.5080573811056723 116 17 3.9961422447950703 116 18 3.4577855392450623 116 19 3.7100929934721343 116 20 2.955331175153404 116 21 4.668148487794539 116 22 5.730635836716814 116 23 5.023119313356652 116 24 5.372169916182703 116 25 4.481111848734869 116 26 2.5427709284261866 116 27 3.086688790388237 116 28 3.030051722253255 116 29 3.8976543024493076 116 30 3.5095414333733608 116 31 2.83984411970534 116 32 2.6083848162996937 116 33 2.4331421841469734 116 34 2.108934495300712 116 35 2.319811977811522 116 36 3.5248076787870097 116 37 3.594055756404231 116 38 4.170630044601687 116 39 5.755769360272538 116 40 6.630438360039669 116 41 6.689366211258651 116 42 4.533962687226037 116 43 -1.0181535937208501 116 44 -1.421286590760932 116 45 2.8560618861994627 116 46 -1.4062243356385693 116 47 -6.126810286505421 116 48 -0.7544241146956416 116 49 -4.1769362080272785 116 50 -0.7121786530874914 116 51 2.150427888195537 116 52 2.1102730812883017 116 53 2.188593397683931 116 54 2.5281217324709964 116 55 1.4770445438148734 116 56 5.055739891927146 117 1 4.29762458198271 117 2 4.045314636228163 117 3 4.100083527527914 117 4 4.377867066104066 117 5 4.134724095118578 117 6 7.176089772073357 117 7 4.133358120385459 117 8 4.3836793463649 117 9 4.439046867647804 117 10 4.455266332512831 117 11 1.6395735473882596 117 12 -6.321845728345142 117 13 -5.986625378484373 117 14 -1.9556453240349327 117 15 -8.683721785776108 117 16 -9.21008607880994 117 17 -1.0532248438125258 117 18 -3.681033026155781 117 19 -0.793704474882297 117 20 -2.1410823269562975 117 21 4.31477296036838 117 22 5.449412208024832 117 23 4.4874653049178335 117 24 4.682085527997042 117 25 4.852841806540781 117 26 4.991505105641373 117 27 4.214747862118195 117 28 4.650726827738488 117 29 4.878455990213016 117 30 5.289921180208804 117 31 3.6510255543044963 117 32 3.7959375589157283 117 33 4.341159061784104 117 34 4.715896520617168 117 35 4.416858391313628 117 36 5.232824556424145 117 37 6.121903857670048 117 38 6.955651141648104 117 39 6.856191301200809 117 40 9.728961566790723 117 41 5.664471696490718 117 42 3.4759458095889073 117 43 -6.255706336336882 117 44 -2.5970249873854065 117 45 4.4843319283935585 117 46 -2.244831930575277 117 47 -0.5943208350286291 117 48 -0.93206124549733 117 49 -1.0119573050736932 117 50 -0.13074405393137312 117 51 5.893254892414948 117 52 6.050990380866359 117 53 6.0139098533801345 117 54 6.722003589011081 117 55 6.228732307527956 117 56 8.7831660522647 118 1 4.646604173819441 118 2 4.85960035051388 118 3 4.936137949296622 118 4 4.952356862022434 118 5 5.701390761785248 118 6 8.145137391120972 118 7 4.741521385691584 118 8 4.830618121875105 118 9 4.713196527511748 118 10 5.156967012784936 118 11 1.9667844317419991 118 12 -6.26538314331113 118 13 -2.3240403444707667 118 14 -6.835237160769626 118 15 -4.88610273815706 118 16 -8.337977235272525 118 17 0.6386118908813536 118 18 -6.600080645203398 118 19 -0.8181942708006638 118 20 -0.46523198682024614 118 21 4.282460035198312 118 22 5.6273033644874175 118 23 4.075560543013069 118 24 5.1314052558882 118 25 4.47801187456799 118 26 4.849668370947498 118 27 4.916448542390299 118 28 4.30310778011944 118 29 5.674714493614378 118 30 5.164410976127169 118 31 4.047284057705852 118 32 3.9119239534735506 118 33 4.428233891716083 118 34 4.523039377760025 118 35 3.97468152056533 118 36 4.621940202682648 118 37 5.148434469914946 118 38 5.802249781103882 118 39 6.2248987841940036 118 40 8.437464968151268 118 41 8.112771016218609 118 42 7.722544449044687 118 43 0.14769502420733716 118 44 0.043451203090786095 118 45 5.8387536971010405 118 46 -1.2703421346569108 118 47 -3.6364977057769288 118 48 0.027802700080901133 118 49 -2.2983518628968227 118 50 0.3400042453883536 118 51 5.073186865204061 118 52 4.707793101954795 118 53 3.8669710778699304 118 54 5.399214473364822 118 55 5.169888770112987 118 56 8.222621834577627 119 1 4.1459004440516765 119 2 4.903935325883339 119 3 4.44950881488424 119 4 4.883039479897171 119 5 5.408287313509383 119 6 7.859423105406687 119 7 4.540254672109597 119 8 4.535403484295934 119 9 5.034449166498376 119 10 4.566185872742718 119 11 1.4453206738250373 119 12 -5.70345492374744 119 13 -0.5854759531970153 119 14 -6.993576358517689 119 15 -10.169353969684156 119 16 -9.483649297200746 119 17 -2.056673119674592 119 18 -7.8833318767304945 119 19 -2.1626699921236785 119 20 -2.83820876373791 119 21 4.317646523586767 119 22 5.13504439193288 119 23 4.417925075032777 119 24 2.5504763325947444 119 25 3.8689337605637704 119 26 4.1311602780551695 119 27 4.22221912648601 119 28 3.7242900461292905 119 29 5.264088174121067 119 30 4.991645318139835 119 31 3.205623255453922 119 32 3.537891581904237 119 33 3.252653314657671 119 34 2.9072758309619964 119 35 3.6467434487848926 119 36 4.016732602401152 119 37 4.356386616290742 119 38 3.5700189577400536 119 39 4.260789002350233 119 40 6.431835130009112 119 41 3.238034914881524 119 42 2.7661756946463782 119 43 -5.516625876566767 119 44 -3.227484757500349 119 45 1.6751365260947075 119 46 -3.012073309885622 119 47 -4.119608191350466 119 48 -5.367693429405376 119 49 -3.283221672889784 119 50 -1.6226980769198782 119 51 3.496128455633336 119 52 3.0860478521307293 119 53 3.886323646483582 119 54 2.7398196809651054 119 55 3.9971231121256565 119 56 4.675120075253204 120 1 4.5944499788081075 120 2 4.621505112418639 120 3 4.387385114829499 120 4 4.473898812135815 120 5 4.479168539563027 120 6 8.002280248263833 120 7 4.127008914036253 120 8 4.2773301400156925 120 9 4.640634169235106 120 10 3.60367903092553 120 11 0.9395735473882567 120 12 -3.73930604580546 120 13 -4.421546013405006 120 14 -4.0143754827650895 120 15 -1.1559440079983325 120 16 -9.483101951825812 120 17 -0.6833835739712555 120 18 -4.600080645203398 120 19 -1.6730695542473768 120 20 -0.8212410571150279 120 21 1.6520745476699652 120 22 3.915285223897847 120 23 3.4088938763464043 120 24 2.990815686727206 120 25 2.1083973620963334 120 26 3.536743200879471 120 27 3.5695097668800955 120 28 1.7792982563099144 120 29 3.8697258314828638 120 30 4.273254513542138 120 31 3.0676922209711606 120 32 2.9300645430427146 120 33 1.9633812840063314 120 34 2.6976425523632024 120 35 2.718445692900932 120 36 3.023300746900336 120 37 2.7107927465589405 120 38 2.337397173394134 120 39 4.249842094851601 120 40 4.305945693774849 120 41 -7.445052113033091 120 42 -5.885165301522203 120 43 -9.101738082368628 120 44 -15.369247209607627 120 45 -7.056937912876286 120 46 -9.526577962321308 120 47 -8.845114485822279 120 48 -7.108251721687807 120 49 -14.32783032094671 120 50 -6.578363101550421 120 51 -1.6297609806009277 120 52 -1.902184222308243 120 53 -1.230534591064309 120 54 -1.3264091094016166 120 55 -1.8323788035831576 120 56 1.6466581157567646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2202.0 5 41.0 6 197.0 7 158.0 8 179.0 9 157.0 10 168.0 11 169.0 12 176.0 13 210.0 14 219.0 15 302.0 16 366.0 17 508.0 18 679.0 19 795.0 20 1101.0 21 1407.0 22 1636.0 23 1733.0 24 1983.0 25 2132.0 26 2214.0 27 2283.0 28 2491.0 29 2586.0 30 2867.0 31 3035.0 32 3461.0 33 3747.0 34 4146.0 35 4663.0 36 5042.0 37 5400.0 38 4550.0 39 2709.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.38823959094229 0.2526174823472121 0.3363160457755052 0.022826880934989045 8 99.43693693693693 0.35762113464816164 0.14304845385926468 0.06239347455563672 9 98.62427904003896 0.4398045989255984 0.41545555538646495 0.5204608056489781 10 53.00553932310689 22.062941319698076 17.976929632335036 6.9545897248599955 11 34.605551497443386 27.253774044314582 17.322559045532017 20.818115412710007 12 30.845203311419528 20.005478451424395 18.990443145848552 30.158875091307525 13 22.37490869247626 13.804175797419042 22.142074506939373 41.67884100316533 14 21.94119795471147 25.09435110786462 30.199963476990504 22.764487460433404 15 36.971744847409944 16.2849367011192 22.49250810347991 24.25081034799095 16 34.67125498675275 23.4887474495234 21.96455218198983 19.875445381734018 17 27.87054937360145 18.064329532826935 35.11180795518548 18.95331313838613 18 25.773070367664964 18.48064280496713 27.515522279035793 28.230764548332115 19 31.292247731563243 21.659764935144025 19.319773460812375 27.72821387248036 20 26.237752007680232 26.8427228258385 27.213019825366107 19.706505341115157 21 24.24860373453455 19.613154570772018 29.640395063231423 26.497846631462007 22 23.103847090333577 18.194545897248602 30.720416362308256 27.981190650109568 23 24.33345507669832 20.09678597516435 27.631178475773073 27.938580472364254 24 25.26519244524936 20.122665773814052 27.042780830048546 27.569360950888033 25 30.3069501301152 20.1320935611998 25.58171386830211 23.97924244038289 26 30.539651601900353 20.08618589353149 21.054635156535507 28.319527348032647 27 22.68297140072488 25.765236195291326 19.78497243626839 31.76681996771541 28 25.74572350398734 31.20015827600901 20.006696292688865 23.047421927314787 29 36.03121618120018 24.447086438947064 18.55594677397229 20.96575060588047 30 28.584480448288463 20.26586673163601 30.437629431112196 20.71202338896333 31 30.516110375477528 28.768853781410286 21.169505197631764 19.54553064548042 32 38.270928462709286 22.409436834094368 20.328767123287673 18.990867579908677 33 30.400731372847783 22.344964193204326 21.53283559347859 25.721468840469296 34 23.484028360143324 26.7149500648014 30.566440497064878 19.234581077990395 35 22.593083687683844 24.386935515827656 35.48839406824867 17.53158672823983 36 25.985079171741777 30.730816077953715 23.888550548112057 19.395554202192447 37 28.49260783417162 22.635269013869838 29.32632220698064 19.545800944977902 38 33.48481844661675 24.374361398746437 22.879843838165097 19.260976316471716 39 28.413402203227133 22.515274793923602 20.04845271289482 29.022870289954444 40 22.352453860669193 17.673319730604607 27.24483377567771 32.72939263304849 41 19.112402385783682 16.22240669003259 25.121441308491665 39.543749615692064 42 26.1112467944804 24.13145683233606 22.36384173891806 27.39345463426548 43 19.18441447488863 20.755782022334778 31.415146152437906 28.644657350338683 44 21.274124784156722 22.524105682981617 24.86208951574701 31.339680017114652 45 25.517735687925093 21.1105011132461 31.06566627016805 22.30609692866075 46 20.22615941033741 26.099894703108546 23.59871202063209 30.07523386592196 47 17.82262996941896 26.084097859327215 24.15596330275229 31.93730886850153 48 25.055965887459074 26.262087870250518 24.685905733648063 23.996040508642352 49 21.314251712616144 26.376577208855256 22.97575637367835 29.333414704850252 50 26.176972801561167 23.23454079765825 25.295767776558115 25.292718624222466 51 20.576200800024427 27.82680387187395 27.03899355705518 24.558001771046445 52 20.345211411493832 26.054007898388303 31.18643550920208 22.414345180915788 53 20.639548195069832 24.42646722124704 32.67953903686179 22.254445546821337 54 21.014813345741352 26.13769851561427 28.764740880867745 24.082747257776624 55 20.028396079509022 26.06790632347104 27.402216726206834 26.501480870813104 56 25.560709163589756 21.616673278203287 28.361966372707574 24.460651185499373 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 10.5 11 20.0 12 33.0 13 48.5 14 51.0 15 55.0 16 59.0 17 65.0 18 71.0 19 74.0 20 77.0 21 80.5 22 95.0 23 106.0 24 131.5 25 157.0 26 195.0 27 233.0 28 246.0 29 282.0 30 305.0 31 365.0 32 425.0 33 482.5 34 540.0 35 688.5 36 837.0 37 869.0 38 957.5 39 1014.0 40 1113.0 41 1212.0 42 1417.5 43 1623.0 44 2122.5 45 2622.0 46 4154.5 47 5407.0 48 5127.0 49 4428.0 50 3729.0 51 3694.5 52 3660.0 53 3879.5 54 4311.0 55 4523.0 56 4374.5 57 4226.0 58 4389.5 59 4553.0 60 4610.5 61 4668.0 62 4423.0 63 3633.5 64 3089.0 65 2714.5 66 2340.0 67 1952.0 68 1564.0 69 1374.5 70 1185.0 71 999.0 72 741.0 73 669.0 74 519.5 75 370.0 76 285.5 77 201.0 78 167.5 79 157.5 80 181.0 81 117.0 82 53.0 83 35.0 84 17.0 85 10.0 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.001521792062332603 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.46871195519844167 16 0.05782809836863891 17 0.028914049184319456 18 0.0 19 0.04261017774531288 20 0.13543949354760165 21 0.001521792062332603 22 0.0 23 0.0 24 0.0076089603116630145 25 0.001521792062332603 26 0.060871682493304116 27 0.07000243486729973 28 0.006087168249330412 29 0.1597881665449233 30 0.060871682493304116 31 0.013696128560993425 32 0.018261504747991236 33 0.12478694911127344 34 0.19326759191624057 35 0.15065741417092768 36 0.048697345994643294 37 0.1552227903579255 38 0.2115290966642318 39 0.12326515704894082 40 0.805028000973947 41 1.004382761139518 42 0.30435841246652057 43 0.24957389822254689 44 0.4124056488921354 45 0.2100073046018992 46 0.27848794740686633 47 0.4747991234477721 48 0.07152422692963234 49 0.2571828585342099 50 0.18261504747991236 51 0.32566350133917704 52 0.19631117604090578 53 0.29979303627952275 54 0.24805210616021425 55 0.3195763330898466 56 0.25870465059654246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 65712.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.71901631361091 #Duplication Level Percentage of deduplicated Percentage of total 1 89.02138855881854 63.845264183102024 2 6.46749278560516 9.276844411979548 3 1.9033271091495503 4.095142439737034 4 0.8105584790358173 2.3252982712442174 5 0.4498387370565269 1.613099586072559 6 0.30342895942963843 1.3056975894813732 7 0.2015786793413682 1.0119917214511809 8 0.14216601595654388 0.8156805454102751 9 0.09548463758275336 0.6163257852447042 >10 0.5559327788151418 7.1980764548332115 >50 0.025462570022067563 1.4046140735329924 >100 0.01909692751655067 3.0222790357925495 >500 0.002121880835172297 0.8035062089116143 >1k 0.002121880835172297 2.66617969320672 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1752 2.66617969320672 No Hit GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 528 0.8035062089116143 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 422 0.6421962503043585 No Hit GTGGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 421 0.6406744582420257 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 209 0.318054541027514 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 190 0.2891404918431945 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 175 0.2663136109082055 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 154 0.23435597759922083 No Hit GTGGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 146 0.22218164110056005 No Hit GTGGTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 135 0.2054419284149014 No Hit GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 134 0.20392013635256878 No Hit GTGGTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 100 0.15217920623326028 No Hit GTGGTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 97 0.14761383004626247 No Hit GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 93 0.14152666179693207 No Hit GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 92 0.14000486973459947 No Hit GTGGTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 90 0.13696128560993426 No Hit GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 71 0.10804723642561481 No Hit GTGGTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 68 0.10348186023861698 No Hit GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 67 0.10196006817628439 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.006087168249330412 0.0 0.0 0.0 0.0 7 0.006087168249330412 0.0 0.0 0.0 0.0 8 0.006087168249330412 0.0 0.0 0.0 0.0 9 0.006087168249330412 0.0 0.0 0.0 0.0 10 0.009130752373995616 0.0 0.0 0.0 0.0 11 0.012174336498660824 0.0 0.0 0.0 0.0 12 0.013696128560993425 0.0 0.0 0.0 0.0 13 0.013696128560993425 0.0 0.0 0.0 0.0 14 0.024348672997321647 0.0 0.0 0.0 0.0 15 0.02587046505965425 0.0 0.0 0.0 0.0 16 0.02739225712198685 0.0 0.0 0.0 0.0 17 0.030435841246652058 0.0 0.0 0.0 0.0 18 0.03195763330898466 0.0 0.0 0.0 0.0 19 0.036523009495982466 0.0 0.0 0.0 0.0 20 0.039566593620647676 0.0 0.0 0.0 0.0 21 0.039566593620647676 0.0 0.0 0.0 0.0 22 0.041088385682980275 0.0 0.0 0.0 0.0 23 0.044131969807645485 0.0 0.0 0.0 0.0 24 0.044131969807645485 0.0 0.0 0.0 0.0 25 0.04717555393231069 0.0 0.0 0.0 0.0 26 0.048697345994643294 0.0 0.0 0.0 0.0 27 0.048697345994643294 0.0 0.0 0.0 0.0 28 0.0517409301193085 0.0 0.0 0.0 0.0 29 0.0517409301193085 0.0 0.0 0.0 0.0 30 0.0547845142439737 0.0 0.0 0.0 0.0 31 0.06391526661796933 0.0 0.0 0.0 0.0 32 0.07152422692963234 0.0 0.0 0.0 0.0 33 0.07304601899196493 0.0 0.0 0.0 0.0 34 0.07304601899196493 0.0 0.0 0.0 0.0 35 0.07456781105429754 0.0 0.0 0.0 0.0 36 0.07608960311663014 0.0 0.0 0.0 0.0 37 0.07913318724129535 0.0 0.0 0.0 0.0 38 0.08065497930362796 0.0 0.0 0.0 0.0 39 0.08065497930362796 0.0 0.0 0.0 0.0 40 0.08065497930362796 0.0 0.0 0.0 0.0 41 0.08369856342829315 0.0 0.0 0.0 0.0 42 0.08826393961529097 0.0 0.0 0.0 0.0 43 0.09435110786462138 0.0 0.0 0.0 0.0 44 0.1050036523009496 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTGAC 30 9.482592E-7 50.620445 41 TTCTGAC 15 0.007995813 50.620445 41 CGCATTG 45 1.200533E-10 50.503094 39 AGCTGTC 15 0.008069594 50.50309 38 GTTCCCT 15 0.008069594 50.50309 39 GTCCAAT 25 1.946731E-5 50.50309 39 TGCCTGT 25 1.9826693E-5 50.347454 37 TATGCCT 30 9.949072E-7 50.269997 35 TGGGCCG 20 4.0456597E-4 50.269997 35 GCTTAGG 15 0.008243719 50.231358 14 ATTGACC 25 2.0099824E-5 50.231358 42 CTTAGGT 15 0.008243719 50.231358 15 CGACTCC 15 0.008268821 50.192776 44 CCCGATC 15 0.008268821 50.192776 49 TTGACCA 15 0.008293979 50.15426 43 GCCCGAT 15 0.008293979 50.15426 48 TACGCTT 15 0.008344466 50.077393 11 ACGCTTA 15 0.008344466 50.077393 12 CCCATCC 15 0.008344466 50.077393 13 CGCTTAG 15 0.008344466 50.077393 13 >>END_MODULE