FastQCFastQC Report
Fri 17 Jun 2016
SRR1526105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12272
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC480.3911342894393742No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC390.31779661016949157No Hit
GTGGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC290.23631029986962193No Hit
GTGGTAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC280.22816166883963493No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG250.20371577574967406No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGATGAGATGGA240.1955671447196871No Hit
GTGGTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC230.18741851368970014No Hit
GTGGTAGGGAGTGCAGTGCTGTGGTAGGGATGAGATGGAC220.17926988265971316No Hit
GTGGTAGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC220.17926988265971316No Hit
GTGGTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC170.13852672750977837No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG160.1303780964797914No Hit
GTGGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC140.11408083441981746No Hit
GTGGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT130.1059322033898305No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA130.1059322033898305No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG130.1059322033898305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCC251.9137967E-434.0000046
GGGATCA251.9137967E-434.0000047
GGGGTGC251.9137967E-434.0000047
TAGGGTG251.9137967E-434.0000045
GGGGAGT251.9137967E-434.0000047
AGGGGTT407.015478E-834.06
AGGGGTG359.792147E-734.06
AGGGGGC359.792147E-734.06
AGGGGAT200.002683170834.06
AGGGGAG455.0258677E-934.06
AGGGGAC359.792147E-734.06
ATGGCCT200.002683170834.015
AGGGTCC200.002683170834.06
AGGGCAG359.792147E-734.06
AGGGATT455.0258677E-934.06
AGGGATC407.015478E-834.06
AGGGAGA200.002683170834.06
AGGGACC200.002683170834.06
AGGGAAT200.002683170834.06
CGGGTAC200.002683170834.034
Produced by FastQC (version 0.11.5)