FastQCFastQC Report
Fri 17 Jun 2016
SRR1526103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4168
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1122.6871401151631478No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT280.6717850287907869No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC260.6238003838771593No Hit
GTGGTAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT220.527831094049904No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG160.3838771593090211No Hit
GTGGTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG150.3598848368522073No Hit
GTGGTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT100.23992322456813817No Hit
GTGGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT90.21593090211132437No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA90.21593090211132437No Hit
GTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG80.19193857965451055No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC70.16794625719769674No Hit
GTGGTAGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT70.16794625719769674No Hit
GTGGTAGGGTACATGGTTGATCCTGCCAGTAGCATATGCT60.14395393474088292No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC60.14395393474088292No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC60.14395393474088292No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG60.14395393474088292No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC50.11996161228406908No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGTCACAGATCC50.11996161228406908No Hit
GTGGTAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT50.11996161228406908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG700.034.329276
AGGGATG200.002364214534.329276
GTAGGGA800.034.329274
TAGGGTA200.002364214534.329275
TAGGGGT200.002364214534.329275
TAGGGGA551.2732926E-1134.329275
TAGGGCA200.002364214534.329275
TAGGGAT502.0736479E-1034.329275
AGGGGAG301.0823749E-534.3292666
GTAGGGC301.0823749E-534.3292664
TAGGGGG1200.034.3292665
TGGTTGA200.002512795433.91566514
TAAGAGC200.002512795433.91566534
CTGGTTG200.002512795433.91566513
GTAGGGG2250.033.5663954
TGGTAGG4100.033.491972
GTGGTAG4100.033.491971
GGTAGGG3950.033.4601753
GTAGGGT603.6379788E-1131.4684944
AGGGGGA353.1108895E-529.4250876
Produced by FastQC (version 0.11.5)