##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4168
Sequences flagged as poor quality	0
Sequence length	40
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	15.837811900191939	4.0	4.0	30.0	4.0	34.0
2	12.470009596928982	4.0	4.0	23.0	4.0	32.0
3	12.419145873320538	4.0	4.0	23.0	4.0	33.0
4	11.50767754318618	4.0	4.0	19.0	4.0	32.0
5	10.797024952015356	4.0	4.0	17.0	4.0	30.0
6	9.551823416506718	4.0	4.0	11.0	4.0	28.0
7	10.628358925143955	4.0	4.0	19.0	4.0	30.0
8	10.639875239923224	4.0	4.0	19.0	4.0	30.0
9	10.515355086372361	4.0	4.0	17.0	4.0	31.0
10	9.700095969289828	4.0	4.0	13.0	4.0	28.0
11	9.898032629558541	4.0	4.0	10.0	4.0	31.0
12	9.482485604606525	4.0	4.0	9.0	4.0	29.0
13	8.841650671785029	4.0	4.0	5.0	4.0	26.0
14	9.242802303262955	4.0	4.0	4.0	4.0	30.0
15	9.077975047984644	4.0	4.0	4.0	4.0	30.0
16	7.6252399232245685	4.0	4.0	4.0	4.0	23.0
17	7.245441458733206	4.0	4.0	4.0	4.0	20.0
18	7.233685220729367	4.0	4.0	4.0	4.0	21.0
19	6.88915547024952	4.0	4.0	4.0	4.0	18.0
20	7.011276391554702	4.0	4.0	4.0	4.0	20.0
21	6.666506717850288	4.0	4.0	4.0	4.0	18.0
22	6.539107485604607	4.0	4.0	4.0	4.0	17.0
23	6.522552783109405	4.0	4.0	4.0	4.0	17.0
24	6.345489443378119	4.0	4.0	4.0	4.0	16.0
25	6.552783109404991	4.0	4.0	4.0	4.0	17.0
26	6.599088291746641	4.0	4.0	4.0	4.0	17.0
27	6.551823416506718	4.0	4.0	4.0	4.0	16.0
28	6.7036948176583495	4.0	4.0	4.0	4.0	18.0
29	6.4095489443378115	4.0	4.0	4.0	4.0	16.0
30	6.5002399232245685	4.0	4.0	4.0	4.0	16.0
31	6.930902111324376	4.0	4.0	4.0	4.0	21.0
32	6.882917466410748	4.0	4.0	4.0	4.0	20.0
33	6.750479846449136	4.0	4.0	4.0	4.0	18.0
34	6.778550863723608	4.0	4.0	4.0	4.0	18.0
35	6.770873320537428	4.0	4.0	4.0	4.0	19.0
36	6.808781190019194	4.0	4.0	4.0	4.0	18.0
37	6.560220729366603	4.0	4.0	4.0	4.0	15.0
38	6.516314779270633	4.0	4.0	4.0	4.0	14.0
39	6.482965451055662	4.0	4.0	4.0	4.0	10.0
40	6.456573896353167	4.0	4.0	4.0	4.0	4.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	18.966144527986632
1	2	20.64979114452799
1	3	2.8226148705096037
1	4	22.80672514619883
1	5	22.3756432748538
1	6	-5.48806182121972
1	7	-6.61842940685046
1	8	-6.301436925647451
1	9	-6.290062656641606
1	10	-5.397318295739346
1	11	-6.046942355889723
1	12	-5.678755221386799
1	13	-4.881194653299914
1	14	-5.352994987468671
1	15	-5.485279866332496
1	16	-3.2173391812865484
1	17	-3.0615121136173755
1	18	-3.0297911445279873
1	19	-2.764945697577277
1	20	-2.8249039264828744
1	21	-2.453395989974938
1	22	-2.4823600668337518
1	23	-2.314816207184628
1	24	-2.3022932330827066
1	25	-2.5084628237259805
1	26	-2.3493149540517955
1	27	-2.3292230576441106
1	28	-2.122355889724311
1	29	-1.9663659147869677
1	30	-2.060705931495404
1	31	-2.260434419381787
1	32	-2.607297410192148
1	33	-2.414314954051795
1	34	-2.4145363408521305
1	35	-2.5122598162071856
1	36	-2.598492063492065
1	37	-2.0667251461988307
1	38	-2.3022013366750214
1	39	-2.0948454469507096
1	40	-2.1039181286549704
2	1	3.537573099415205
2	2	-6.3502088554720135
2	3	-2.8202422723475387
2	4	-4.550417710944029
2	5	-3.3386424394319123
2	6	-3.3452046783625766
2	7	-1.3327151211361734
2	8	-2.0157226399331645
2	9	-5.147205513784463
2	10	-1.6830325814536318
2	11	-3.4755137844611514
2	12	-0.535898078529657
2	13	1.1188053467000856
2	14	-1.7815664160401
2	15	1.6575772765246466
2	16	-0.6459106098579772
2	17	-1.4900835421888043
2	18	0.6844945697577272
2	19	3.5207685881370097
2	20	0.4608103592314112
2	21	3.8323182957393485
2	22	-0.625217209690895
2	23	3.8280409356725142
2	24	0.5548496240601501
2	25	3.0629657477025907
2	26	0.9363993316624901
2	27	4.670776942355889
2	28	-0.12235588972431088
2	29	1.4622055137844612
2	30	1.6535797827903105
2	31	3.739565580618213
2	32	4.106988304093566
2	33	4.2999707602339186
2	34	3.0140350877192983
2	35	4.916311612364243
2	36	2.972936507936506
2	37	6.933274853801169
2	38	5.983512949039265
2	39	6.4765831244778616
2	40	5.467510442773601
3	1	4.466144527986632
3	2	-2.3502088554720135
3	3	3.3226148705096037
3	4	2.3067251461988274
3	5	1.6256432748538021
3	6	2.01193817878028
3	7	1.6315705931495401
3	8	10.698563074352549
3	9	9.709937343358394
3	10	1.6026817042606538
3	11	5.703057644110277
3	12	7.571244778613201
3	13	4.618805346700086
3	14	-0.3529949874686711
3	15	6.264720133667504
3	16	7.282660818713452
3	17	6.1884878863826245
3	18	7.220208855472013
3	19	2.735054302422723
3	20	8.175096073517125
3	21	6.796604010025062
3	22	2.7676399331662482
3	23	8.685183792815373
3	24	2.1977067669172934
3	25	-0.7584628237259805
3	26	2.4006850459482045
3	27	1.1707769423558894
3	28	3.627644110275689
3	29	0.7836340852130323
3	30	1.689294068504596
3	31	4.989565580618213
3	32	2.892702589807852
3	33	2.835685045948205
3	34	4.8354636591478695
3	35	2.2377401837928144
3	36	-0.09849206349206518
3	37	1.6832748538011693
3	38	4.947798663324979
3	39	5.15515455304929
3	40	5.14608187134503
4	1	-12.033855472013368
4	2	-9.350208855472014
4	3	-8.677385129490396
4	4	-7.693274853801173
4	5	-6.624356725146198
4	6	-5.48806182121972
4	7	-6.61842940685046
4	8	-6.301436925647451
4	9	-6.290062656641606
4	10	-5.397318295739346
4	11	-6.046942355889723
4	12	-5.678755221386799
4	13	-4.881194653299914
4	14	-5.352994987468671
4	15	-5.485279866332496
4	16	-3.2173391812865484
4	17	-3.0615121136173755
4	18	-3.0297911445279873
4	19	-2.764945697577277
4	20	-2.8249039264828744
4	21	-2.453395989974938
4	22	-2.4823600668337518
4	23	-2.314816207184628
4	24	-2.3022932330827066
4	25	-2.5084628237259805
4	26	-2.3493149540517955
4	27	-2.3292230576441106
4	28	-2.122355889724311
4	29	-1.9663659147869677
4	30	-2.060705931495404
4	31	-2.260434419381787
4	32	-2.607297410192148
4	33	-2.414314954051795
4	34	-2.4145363408521305
4	35	-2.5122598162071856
4	36	-2.598492063492065
4	37	-2.0667251461988307
4	38	-2.3022013366750214
4	39	-2.0948454469507096
4	40	-2.1039181286549704
5	1	-2.1449665831244786
5	2	-3.905764411027569
5	3	-2.121829573934841
5	4	-2.248830409356728
5	5	-2.1799122807017532
5	6	0.2897159565580587
5	7	-3.285096073517127
5	8	-1.0792147034252295
5	9	-0.7345071010860504
5	10	0.7137928153717645
5	11	0.1752798663324988
5	12	-3.2343107769423547
5	13	1.1188053467000856
5	14	1.5358939014202182
5	15	-2.2630576441102734
5	16	-3.2173391812865484
5	17	-3.0615121136173755
5	18	-3.0297911445279873
5	19	-2.764945697577277
5	20	-2.8249039264828744
5	21	-2.453395989974938
5	22	-2.4823600668337518
5	23	-2.314816207184628
5	24	-2.3022932330827066
5	25	-2.5084628237259805
5	26	-2.3493149540517955
5	27	-2.3292230576441106
5	28	-2.122355889724311
5	29	-1.9663659147869677
5	30	-2.060705931495404
5	31	-2.260434419381787
5	32	-2.607297410192148
5	33	-2.414314954051795
5	34	-2.4145363408521305
5	35	-2.5122598162071856
5	36	-2.598492063492065
5	37	-2.0667251461988307
5	38	-2.3022013366750214
5	39	-2.0948454469507096
5	40	-2.1039181286549704
7	1	18.966144527986632
7	2	10.649791144527986
7	3	22.322614870509604
7	4	21.30672514619883
7	5	19.3756432748538
7	6	20.51193817878028
7	7	19.38157059314954
7	8	22.69856307435255
7	9	6.709937343358394
7	10	17.602681704260654
7	11	-2.0469423558897226
7	12	18.3212447786132
7	13	2.1188053467000856
7	14	16.64700501253133
7	15	1.5147201336675042
7	16	-0.2173391812865484
7	17	7.9384878863826245
7	18	17.970208855472013
7	19	11.235054302422723
7	20	10.175096073517125
7	21	19.546604010025064
7	22	18.517639933166247
7	23	10.685183792815373
7	24	4.697706766917293
7	25	16.49153717627402
7	26	19.650685045948205
7	27	2.6707769423558894
7	28	6.877644110275689
7	29	12.033634085213032
7	30	22.939294068504594
7	31	19.739565580618212
7	32	24.392702589807854
7	33	15.585685045948205
7	34	24.58546365914787
7	35	24.487740183792816
7	36	29.401507936507933
7	37	24.93327485380117
7	38	24.697798663324978
7	39	22.90515455304929
7	40	26.89608187134503
8	1	-2.3195697577276544
8	2	-3.6359231411863
8	3	0.3226148705096037
8	4	1.8781537176273986
8	5	5.089928989139516
8	6	-1.2023475355054334
8	7	3.38157059314954
8	8	2.698563074352549
8	9	3.138508771929823
8	10	3.602681704260654
8	11	4.667343358395991
8	12	6.035530492898914
8	13	5.261662489557228
8	14	3.5041478696741866
8	15	-0.3424227234753534
8	16	1.3540893901420228
8	17	3.652773600668338
8	18	1.541637426900584
8	19	-0.47923141186299123
8	20	3.0322389306599833
8	21	1.6894611528822043
8	22	2.374782790309106
8	23	2.5423266499582295
8	24	-1.5880075187969922
8	25	0.4915371762740195
8	26	2.6506850459482045
8	27	0.5279197994987461
8	28	1.306215538847118
8	29	2.0336340852130323
8	30	1.939294068504596
8	31	4.882422723475355
8	32	3.1069883040935657
8	33	1.585685045948205
8	34	4.299749373433583
8	35	3.4877401837928144
8	36	5.687222222222221
8	37	3.790417710944027
8	38	6.1263700918964075
8	39	5.190868838763577
8	40	5.467510442773601
9	1	6.966144527986632
9	2	5.7747911445279865
9	3	4.322614870509604
9	4	4.431725146198827
9	5	7.625643274853802
9	6	0.26193817878028014
9	7	7.50657059314954
9	8	-1.676436925647451
9	9	2.209937343358394
9	10	4.227681704260654
9	11	1.9530576441102774
9	12	5.321244778613201
9	13	4.743805346700086
9	14	1.022005012531329
9	15	3.6397201336675042
9	16	6.657660818713452
9	17	0.06348788638262448
9	18	6.220208855472013
9	19	2.860054302422723
9	20	7.800096073517126
9	21	6.421604010025062
9	22	9.642639933166247
9	23	4.685183792815372
9	24	6.197706766917293
9	25	4.3665371762740195
9	26	8.025685045948205
9	27	4.295776942355889
9	28	10.00264411027569
9	29	5.908634085213032
9	30	7.064294068504596
9	31	10.864565580618212
9	32	5.392702589807852
9	33	7.335685045948205
9	34	7.4604636591478695
9	35	4.862740183792814
9	36	8.401507936507935
9	37	8.55827485380117
9	38	3.5727986633249786
9	39	5.15515455304929
9	40	4.39608187134503
10	1	10.966144527986632
10	2	10.649791144527986
10	3	-0.6773851294903963
10	4	2.3067251461988274
10	5	-4.624356725146198
10	6	12.51193817878028
10	7	8.38157059314954
10	8	10.698563074352549
10	9	12.709937343358394
10	10	10.602681704260654
10	11	0.9530576441102774
10	12	12.3212447786132
10	13	-0.8811946532999144
10	14	8.647005012531329
10	15	20.514720133667502
10	16	22.782660818713452
10	17	15.938487886382624
10	18	15.970208855472013
10	19	20.235054302422725
10	20	23.175096073517125
10	21	20.546604010025064
10	22	18.517639933166247
10	23	23.685183792815373
10	24	23.697706766917292
10	25	26.49153717627402
10	26	0.6506850459482045
10	27	24.67077694235589
10	28	-2.122355889724311
10	29	-1.9663659147869677
10	30	-2.060705931495404
10	31	-2.260434419381787
10	32	-2.607297410192148
10	33	-2.414314954051795
10	34	-2.4145363408521305
10	35	-2.5122598162071856
10	36	-2.598492063492065
10	37	-2.0667251461988307
10	38	-2.3022013366750214
10	39	-2.0948454469507096
10	40	-2.1039181286549704
11	1	8.966144527986632
11	2	19.64979114452799
11	3	22.322614870509604
11	4	21.30672514619883
11	5	-6.624356725146198
11	6	-5.48806182121972
11	7	-6.61842940685046
11	8	-6.301436925647451
11	9	-6.290062656641606
11	10	-5.397318295739346
11	11	-6.046942355889723
11	12	-5.678755221386799
11	13	-4.881194653299914
11	14	-5.352994987468671
11	15	-5.485279866332496
11	16	-3.2173391812865484
11	17	-3.0615121136173755
11	18	-3.0297911445279873
11	19	-2.764945697577277
11	20	-2.8249039264828744
11	21	-2.453395989974938
11	22	-2.4823600668337518
11	23	-2.314816207184628
11	24	-2.3022932330827066
11	25	-2.5084628237259805
11	26	-2.3493149540517955
11	27	-2.3292230576441106
11	28	-2.122355889724311
11	29	-1.9663659147869677
11	30	-2.060705931495404
11	31	-2.260434419381787
11	32	-2.607297410192148
11	33	-2.414314954051795
11	34	-2.4145363408521305
11	35	-2.5122598162071856
11	36	-2.598492063492065
11	37	-2.0667251461988307
11	38	-2.3022013366750214
11	39	-2.0948454469507096
11	40	-2.1039181286549704
12	1	-12.033855472013368
12	2	-9.350208855472014
12	3	-8.677385129490396
12	4	-7.693274853801173
12	5	-6.624356725146198
12	6	-5.48806182121972
12	7	-6.61842940685046
12	8	-6.301436925647451
12	9	-6.290062656641606
12	10	-5.397318295739346
12	11	-6.046942355889723
12	12	-5.678755221386799
12	13	-4.881194653299914
12	14	-5.352994987468671
12	15	-5.485279866332496
12	16	-3.2173391812865484
12	17	-3.0615121136173755
12	18	-3.0297911445279873
12	19	-2.764945697577277
12	20	-2.8249039264828744
12	21	-2.453395989974938
12	22	-2.4823600668337518
12	23	-2.314816207184628
12	24	-2.3022932330827066
12	25	-2.5084628237259805
12	26	-2.3493149540517955
12	27	-2.3292230576441106
12	28	-2.122355889724311
12	29	-1.9663659147869677
12	30	-2.060705931495404
12	31	-2.260434419381787
12	32	-2.607297410192148
12	33	-2.414314954051795
12	34	-2.4145363408521305
12	35	-2.5122598162071856
12	36	-2.598492063492065
12	37	-2.0667251461988307
12	38	-2.3022013366750214
12	39	-2.0948454469507096
12	40	-2.1039181286549704
13	1	9.966144527986632
13	2	15.649791144527986
13	3	14.322614870509604
13	4	1.3067251461988274
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112	35	-2.5122598162071856
112	36	-2.598492063492065
112	37	-2.0667251461988307
112	38	-2.3022013366750214
112	39	-2.0948454469507096
112	40	-2.1039181286549704
114	1	-12.033855472013368
114	2	-9.350208855472014
114	3	-8.677385129490396
114	4	-7.693274853801173
114	5	-6.624356725146198
114	6	-5.48806182121972
114	7	-6.61842940685046
114	8	-6.301436925647451
114	9	-6.290062656641606
114	10	-5.397318295739346
114	11	-6.046942355889723
114	12	-5.678755221386799
114	13	-4.881194653299914
114	14	-5.352994987468671
114	15	-5.485279866332496
114	16	-3.2173391812865484
114	17	-3.0615121136173755
114	18	-3.0297911445279873
114	19	-2.764945697577277
114	20	-2.8249039264828744
114	21	-2.453395989974938
114	22	-2.4823600668337518
114	23	-2.314816207184628
114	24	-2.3022932330827066
114	25	-2.5084628237259805
114	26	-2.3493149540517955
114	27	-2.3292230576441106
114	28	-2.122355889724311
114	29	-1.9663659147869677
114	30	-2.060705931495404
114	31	-2.260434419381787
114	32	-2.607297410192148
114	33	-2.414314954051795
114	34	-2.4145363408521305
114	35	-2.5122598162071856
114	36	-2.598492063492065
114	37	-2.0667251461988307
114	38	-2.3022013366750214
114	39	-2.0948454469507096
114	40	-2.1039181286549704
116	1	-12.033855472013368
116	2	-9.350208855472014
116	3	-8.677385129490396
116	4	-7.693274853801173
116	5	-6.624356725146198
116	6	-5.48806182121972
116	7	-6.61842940685046
116	8	-6.301436925647451
116	9	-6.290062656641606
116	10	-5.397318295739346
116	11	-6.046942355889723
116	12	-5.678755221386799
116	13	-4.881194653299914
116	14	-5.352994987468671
116	15	-5.485279866332496
116	16	-3.2173391812865484
116	17	-3.0615121136173755
116	18	-3.0297911445279873
116	19	-2.764945697577277
116	20	-2.8249039264828744
116	21	-2.453395989974938
116	22	-2.4823600668337518
116	23	-2.314816207184628
116	24	-2.3022932330827066
116	25	-2.5084628237259805
116	26	-2.3493149540517955
116	27	-2.3292230576441106
116	28	-2.122355889724311
116	29	-1.9663659147869677
116	30	-2.060705931495404
116	31	-2.260434419381787
116	32	-2.607297410192148
116	33	-2.414314954051795
116	34	-2.4145363408521305
116	35	-2.5122598162071856
116	36	-2.598492063492065
116	37	-2.0667251461988307
116	38	-2.3022013366750214
116	39	-2.0948454469507096
116	40	-2.1039181286549704
117	1	16.466144527986632
117	2	15.149791144527986
117	3	12.322614870509604
117	4	18.30672514619883
117	5	9.375643274853802
117	6	9.51193817878028
117	7	-4.11842940685046
117	8	14.198563074352549
117	9	19.209937343358394
117	10	5.102681704260654
117	11	13.453057644110277
117	12	12.3212447786132
117	13	13.118805346700086
117	14	9.647005012531329
117	15	15.514720133667504
117	16	8.782660818713452
117	17	6.4384878863826245
117	18	7.470208855472013
117	19	11.735054302422723
117	20	4.175096073517126
117	21	5.046604010025062
117	22	10.517639933166247
117	23	10.685183792815373
117	24	12.197706766917292
117	25	-2.5084628237259805
117	26	-2.3493149540517955
117	27	-2.3292230576441106
117	28	-2.122355889724311
117	29	-1.9663659147869677
117	30	-2.060705931495404
117	31	-2.260434419381787
117	32	-2.607297410192148
117	33	-2.414314954051795
117	34	-2.4145363408521305
117	35	-2.5122598162071856
117	36	-2.598492063492065
117	37	-2.0667251461988307
117	38	-2.3022013366750214
117	39	-2.0948454469507096
117	40	-2.1039181286549704
119	1	18.166144527986635
119	2	20.049791144527987
119	3	19.922614870509605
119	4	20.506725146198832
119	5	12.575643274853801
119	6	11.31193817878028
119	7	9.38157059314954
119	8	16.89856307435255
119	9	16.709937343358394
119	10	8.602681704260654
119	11	0.35305764411027774
119	12	-0.8787552213867986
119	13	0.5188053467000859
119	14	-1.1529949874686718
119	15	0.9147201336675046
119	16	-3.2173391812865484
119	17	-3.0615121136173755
119	18	-3.0297911445279873
119	19	-2.764945697577277
119	20	-2.8249039264828744
119	21	-2.453395989974938
119	22	-2.4823600668337518
119	23	-2.314816207184628
119	24	-2.3022932330827066
119	25	-2.5084628237259805
119	26	-2.3493149540517955
119	27	-2.3292230576441106
119	28	-2.122355889724311
119	29	-1.9663659147869677
119	30	-2.060705931495404
119	31	-2.260434419381787
119	32	-2.607297410192148
119	33	-2.414314954051795
119	34	-2.4145363408521305
119	35	-2.5122598162071856
119	36	-2.598492063492065
119	37	-2.0667251461988307
119	38	-2.3022013366750214
119	39	-2.0948454469507096
119	40	-2.1039181286549704
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2506.0
5	114.0
6	102.0
7	115.0
8	99.0
9	97.0
10	122.0
11	124.0
12	94.0
13	73.0
14	64.0
15	38.0
16	29.0
17	35.0
18	35.0
19	37.0
20	74.0
21	86.0
22	74.0
23	69.0
24	59.0
25	45.0
26	29.0
27	16.0
28	14.0
29	8.0
30	6.0
31	3.0
32	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	98.24771963514164	0.6481036965914546	0.8641382621219394	0.2400384061449832
8	96.92603266090299	1.248799231508165	1.3208453410182517	0.5043227665706052
9	96.99735767475379	1.0088878212827288	1.2010569300984866	0.7926975738650012
10	48.60710854947166	25.048030739673393	17.77137367915466	8.573487031700289
11	33.949136276391556	29.318618042226486	20.60940499040307	16.122840690978887
12	32.55758157389636	23.56046065259117	22.384836852207293	21.497120921305182
13	21.478281737461003	24.862011039116872	25.82193424526038	27.837772978161745
14	20.56142034548944	29.750479846449135	33.10940499040307	16.57869481765835
15	37.020153550863725	22.576775431861805	26.391554702495203	14.011516314779271
16	34.26103646833013	28.095009596928982	24.73608445297505	12.907869481765836
17	27.16582673386129	24.934005279577633	35.157187425006	12.742980561555076
18	26.343570057581573	26.00767754318618	31.09404990403071	16.554702495201536
19	27.936584194090802	30.242613499879894	23.27648330530867	18.544319000720634
20	26.24760076775432	32.02975047984645	27.83109404990403	13.8915547024952
21	20.753358925143953	27.49520153550864	32.12571976967371	19.625719769673704
22	20.657389635316697	23.824376199616122	32.341650671785025	23.17658349328215
23	23.152591170825335	25.74376199616123	28.550863723608444	22.552783109404988
24	24.88003838771593	27.543186180422264	28.766794625719772	18.809980806142036
25	27.950012016342225	25.18625330449411	26.07546262917568	20.78827204998798
26	25.343290773307636	26.860997350036136	24.548301614068897	23.24741026258733
27	19.022393450517697	29.882013002648687	23.814110281724055	27.281483265109557
28	22.48378573144367	34.37424933941869	24.141244294979582	19.000720634158057
29	30.804431599229286	28.80539499036609	22.85645472061657	17.533718689788053
30	23.014158867290615	26.853851691864648	30.8615310775138	19.270458363330935
31	26.39788816894648	30.52555795536357	25.84593232541397	17.23062155027598
32	33.1810531377735	24.90983409473431	23.106515989420533	18.80259677807165
33	26.079654510556622	25.599808061420347	27.47120921305182	20.849328214971212
34	20.89731285988484	30.182341650671784	33.133397312859884	15.786948176583493
35	17.51439539347409	29.870441458733204	36.924184261036466	15.690978886756238
36	19.918308505526188	35.92023065833734	26.54973570398847	17.611725132148006
37	23.704414587332053	26.87140115163148	31.789827255278315	17.634357005758158
38	27.087332053742802	29.750479846449135	26.15163147792706	17.010556621880998
39	23.75239923224568	28.430902111324375	24.06429942418426	23.75239923224568
40	20.062395008399328	26.63786897048236	32.27741780657548	21.02231821454284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	2.5
15	5.0
16	9.0
17	13.0
18	13.0
19	15.5
20	18.0
21	23.0
22	28.0
23	28.0
24	32.5
25	37.0
26	44.5
27	52.0
28	52.0
29	62.0
30	72.0
31	87.0
32	102.0
33	102.0
34	129.0
35	156.0
36	161.5
37	167.0
38	167.0
39	183.5
40	200.0
41	229.5
42	259.0
43	259.0
44	298.0
45	337.0
46	318.5
47	300.0
48	300.0
49	347.0
50	394.0
51	437.0
52	480.0
53	480.0
54	434.0
55	388.0
56	368.0
57	348.0
58	348.0
59	340.5
60	333.0
61	293.0
62	253.0
63	253.0
64	189.0
65	125.0
66	86.0
67	47.0
68	47.0
69	37.5
70	28.0
71	22.0
72	16.0
73	16.0
74	9.5
75	3.0
76	3.0
77	3.0
78	3.0
79	2.5
80	2.0
81	1.5
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.04798464491362764
8	0.09596928982725528
9	0.11996161228406908
10	0.09596928982725528
11	0.0
12	0.0
13	0.02399232245681382
14	0.0
15	0.0
16	0.0
17	0.02399232245681382
18	0.0
19	0.11996161228406908
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.16794625719769674
26	0.4078694817658349
27	0.3598848368522073
28	0.11996161228406908
29	0.3838771593090211
30	0.02399232245681382
31	0.02399232245681382
32	0.21593090211132437
33	0.0
34	0.0
35	0.0
36	0.14395393474088292
37	0.0
38	0.0
39	0.0
40	0.02399232245681382
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4168.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.8598848368522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.70234844347351	83.20537428023033
2	3.823047515019115	6.71785028790787
3	0.7646095030038231	2.015355086372361
4	0.19115237575095578	0.6717850287907869
5	0.0819224467504096	0.3598848368522073
6	0.10922992900054614	0.5758157389635317
7	0.05461496450027307	0.33589251439539347
8	0.027307482250136534	0.19193857965451055
9	0.05461496450027307	0.43186180422264875
>10	0.1638448935008192	2.8071017274472165
>50	0.0	0.0
>100	0.027307482250136534	2.6871401151631478
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	112	2.6871401151631478	No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	28	0.6717850287907869	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	26	0.6238003838771593	No Hit
GTGGTAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT	22	0.527831094049904	No Hit
GTGGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	16	0.3838771593090211	No Hit
GTGGTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	15	0.3598848368522073	No Hit
GTGGTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	10	0.23992322456813817	No Hit
GTGGTAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	9	0.21593090211132437	No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	9	0.21593090211132437	No Hit
GTGGTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG	8	0.19193857965451055	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC	7	0.16794625719769674	No Hit
GTGGTAGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT	7	0.16794625719769674	No Hit
GTGGTAGGGTACATGGTTGATCCTGCCAGTAGCATATGCT	6	0.14395393474088292	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC	6	0.14395393474088292	No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC	6	0.14395393474088292	No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	6	0.14395393474088292	No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	5	0.11996161228406908	No Hit
GTGGTAGGGGAGTGCAGTGCTGTGGTAGGGTCACAGATCC	5	0.11996161228406908	No Hit
GTGGTAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT	5	0.11996161228406908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	70	0.0	34.32927	6
AGGGATG	20	0.0023642145	34.32927	6
GTAGGGA	80	0.0	34.32927	4
TAGGGTA	20	0.0023642145	34.32927	5
TAGGGGT	20	0.0023642145	34.32927	5
TAGGGGA	55	1.2732926E-11	34.32927	5
TAGGGCA	20	0.0023642145	34.32927	5
TAGGGAT	50	2.0736479E-10	34.32927	5
AGGGGAG	30	1.0823749E-5	34.329266	6
GTAGGGC	30	1.0823749E-5	34.329266	4
TAGGGGG	120	0.0	34.329266	5
TGGTTGA	20	0.0025127954	33.915665	14
TAAGAGC	20	0.0025127954	33.915665	34
CTGGTTG	20	0.0025127954	33.915665	13
GTAGGGG	225	0.0	33.566395	4
TGGTAGG	410	0.0	33.49197	2
GTGGTAG	410	0.0	33.49197	1
GGTAGGG	395	0.0	33.460175	3
GTAGGGT	60	3.6379788E-11	31.468494	4
AGGGGGA	35	3.1108895E-5	29.425087	6
>>END_MODULE