FastQCFastQC Report
Fri 17 Jun 2016
SRR1526102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162349
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG46732.878366974850476No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG12260.7551632593979636No Hit
ATACAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT6000.3695741889386445No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG5800.35725504930735635No Hit
ATACAGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT5400.3326167700447801No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG5380.33138485608165125No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4740.2919636092615292No Hit
ATACAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC4510.2777965986855478No Hit
ATACAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC4120.2537742764045359No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG3180.1958743201374816No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3170.1952583631559172No Hit
ATACAGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA2910.1792434816352426No Hit
ATACAGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2700.16630838502239004No Hit
ATACAGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT2510.1546052023726663No Hit
ATACAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC2450.15090946048327986No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2260.1392062778335561No Hit
ATACAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2150.1324307510363476No Hit
ATACAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT2040.12565522423913913No Hit
ATACAGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC2020.12442331027601031No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1750.10779247177377133No Hit
ATACAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT1700.10471268686594928No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1660.10224885893969164No Hit
ATACAGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT1650.10163290195812724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATAT150.00809534850.49655540
GCCCTAA150.00811541250.4649639
CTAGACC204.036227E-450.3389536
CAATTCG150.00822642550.29186242
TCTAGAC204.0549194E-450.2918635
ACCCGAT204.0549194E-450.2918642
ATTATAC204.0549194E-450.2918642
CACGATT1350.050.26051747
CTCCACG1250.050.2448644
ATTCGCC150.00825689750.24485844
AATTCGC150.00825689750.24485843
TCGTTGA355.102811E-850.18232714
ACCTAAA150.00829765750.18232715
GTATGAT204.098803E-450.18232715
TCGCGCA204.1621475E-450.02667650
ACTAATC301.0406275E-650.01116610
GTACCTC355.3067197E-849.9337469
CATATAC204.2133807E-449.90284333
AGGTAAG150.008514103549.8565731
ACCGACG150.008514103549.8565731
Produced by FastQC (version 0.11.5)