##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526102_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	162349
Sequences flagged as poor quality	0
Sequence length	56
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.51422552648923	38.0	36.0	39.0	33.0	40.0
2	35.52768418653641	39.0	36.0	39.0	26.0	40.0
3	36.05923658291705	38.0	36.0	39.0	31.0	40.0
4	35.236749225434096	39.0	35.0	39.0	25.0	40.0
5	35.50783805259041	38.0	36.0	39.0	28.0	40.0
6	34.930877307528846	38.0	35.0	39.0	25.0	40.0
7	35.60571361696099	38.0	36.0	39.0	28.0	40.0
8	35.42971006904878	38.0	35.0	39.0	28.0	40.0
9	35.440569390633755	38.0	36.0	39.0	28.0	40.0
10	34.714448502916554	38.0	35.0	39.0	25.0	40.0
11	34.28654318782376	38.0	33.0	39.0	26.0	40.0
12	33.16617903405626	37.0	32.0	39.0	20.0	40.0
13	32.81806478635532	37.0	31.0	39.0	17.0	40.0
14	33.54924884046098	38.0	33.0	39.0	21.0	40.0
15	32.389894609760454	37.0	31.0	39.0	15.0	40.0
16	31.825727291205983	36.0	29.0	39.0	16.0	40.0
17	30.913809139569693	35.0	27.0	38.0	13.0	40.0
18	30.27216675187405	35.0	25.0	38.0	12.0	40.0
19	31.499128420871088	35.0	28.0	38.0	16.0	40.0
20	32.05405022513228	36.0	29.0	38.0	18.0	40.0
21	32.286210571053715	37.0	30.0	39.0	17.0	40.0
22	31.620564339786508	36.0	29.0	39.0	14.0	40.0
23	32.314113422318584	37.0	30.0	39.0	17.0	40.0
24	31.719160573825523	36.0	29.0	39.0	15.0	40.0
25	32.10472500600558	36.0	30.0	39.0	17.0	40.0
26	32.69206462620651	37.0	32.0	39.0	17.0	40.0
27	32.814966522738054	37.0	32.0	39.0	18.0	40.0
28	32.67972700786577	37.0	32.0	39.0	18.0	40.0
29	32.32889638987613	36.0	31.0	39.0	16.0	40.0
30	31.95709859623404	36.0	30.0	39.0	14.0	40.0
31	32.74115639763719	37.0	32.0	39.0	16.0	40.0
32	32.56581192369525	37.0	32.0	39.0	15.0	40.0
33	32.361381961083836	36.0	32.0	39.0	12.0	40.0
34	32.22233583206549	36.0	31.0	39.0	11.0	40.0
35	32.00445336897671	36.0	31.0	39.0	8.0	40.0
36	31.1238689489926	35.0	30.0	38.0	4.0	40.0
37	30.83544709237507	35.0	29.0	38.0	4.0	40.0
38	30.231445835822825	35.0	28.0	38.0	4.0	39.0
39	29.3252622436849	35.0	26.0	38.0	4.0	39.0
40	27.187571220025994	33.0	17.0	38.0	4.0	39.0
41	25.40319312099243	31.0	13.0	37.0	4.0	39.0
42	26.236157906731794	31.0	18.0	36.0	4.0	39.0
43	26.179058694540775	31.0	17.0	37.0	4.0	39.0
44	25.19653955367757	30.0	12.0	36.0	4.0	39.0
45	26.16479313084774	32.0	17.0	37.0	4.0	39.0
46	24.19019519676746	29.0	10.0	36.0	4.0	39.0
47	23.254993871228034	28.0	5.0	35.0	4.0	39.0
48	23.899167842117908	29.0	4.0	35.0	4.0	39.0
49	22.90331939217365	27.0	4.0	35.0	4.0	39.0
50	23.452297211562744	28.0	4.0	35.0	4.0	39.0
51	23.64959439232764	29.0	4.0	36.0	4.0	39.0
52	23.52067459608621	29.0	4.0	36.0	4.0	39.0
53	23.286666379220076	29.0	4.0	35.0	4.0	39.0
54	22.850156145094825	29.0	4.0	35.0	4.0	38.0
55	22.505959383796636	28.0	4.0	35.0	4.0	38.0
56	21.825283802179257	27.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.609995325916721
1	2	5.012826460395971
1	3	4.142065871600536
1	4	5.187042501806417
1	5	4.684233632023798
1	6	5.595423876156005
1	7	4.7237350073314275
1	8	4.773422797908346
1	9	4.753692991993013
1	10	5.032751321696409
1	11	-1.006908651886917
1	12	-8.183609464231079
1	13	-18.206857363942767
1	14	-7.279854296267413
1	15	0.9385488146513907
1	16	-1.0789452440297964
1	17	-5.513712796404594
1	18	-4.316627361569704
1	19	0.24548693380565112
1	20	-0.15756986779357973
1	21	6.727656447834747
1	22	7.280251463902168
1	23	6.762548825745789
1	24	7.265991601098669
1	25	6.388405385250707
1	26	6.364773001913846
1	27	6.215784963554647
1	28	6.292962702563191
1	29	6.5726185297022575
1	30	6.536167098937582
1	31	5.662628203881873
1	32	5.896571462412638
1	33	5.968967161430573
1	34	5.996116077084302
1	35	5.811595874386104
1	36	5.212675400134842
1	37	5.340410444113054
1	38	5.489780628256796
1	39	4.728083349057297
1	40	-9.137744403778711
1	41	-0.250434047664978
1	42	-1.9989674558513748
1	43	-9.357295573011482
1	44	-14.224440980334792
1	45	-3.2687465845454646
1	46	-5.585007592538814
1	47	-9.87420925355313
1	48	-5.302528445712191
1	49	-12.802085211197088
1	50	-3.619252931248983
1	51	2.1640967839669933
1	52	2.353129006526128
1	53	2.346857419809819
1	54	2.273797737149337
1	55	2.369839150942415
1	56	5.194628941984945
2	1	4.1144239573945285
2	2	5.521353452529517
2	3	4.717367619033432
2	4	5.824179954517639
2	5	5.415423207475669
2	6	5.808193347122199
2	7	4.882865494330765
2	8	5.203510469799291
2	9	5.171996000459956
2	10	5.432829385708899
2	11	4.421797887475357
2	12	0.7899538576929004
2	13	2.373143236549641
2	14	5.456439464616508
2	15	6.314457059412096
2	16	5.919268291068985
2	17	7.6433760164219215
2	18	5.539569720037214
2	19	6.88567188546601
2	20	5.9225658434896715
2	21	7.530349656265663
2	22	8.170286292461583
2	23	7.274228402999135
2	24	7.973236541950165
2	25	7.182916884680239
2	26	6.56451178771821
2	27	6.681797093501203
2	28	6.562223496414152
2	29	6.932448590351992
2	30	7.167434738401944
2	31	6.178015821728508
2	32	5.816375701889008
2	33	6.440293649150501
2	34	5.7644010707791296
2	35	6.2050384973369255
2	36	7.342697017861376
2	37	7.699865197010432
2	38	8.166850825818791
2	39	8.500241518756454
2	40	6.247396411689973
2	41	10.528284501545379
2	42	9.707436795634845
2	43	9.00933506411096
2	44	-1.27943107221013
2	45	9.029803226299432
2	46	7.743656912355199
2	47	3.7727012130294675
2	48	7.22212176746261
2	49	0.5354365566525487
2	50	8.31768035404496
2	51	10.435954707464258
2	52	10.240911988571405
2	53	10.481923173728031
2	54	10.558010791854198
2	55	10.546744213093376
2	56	11.98763921040505
3	1	3.819191209607041
3	2	5.288164500362463
3	3	4.462695284069127
3	4	5.324741436830948
3	5	5.325492456960973
3	6	5.510674564201956
3	7	4.654930412381084
3	8	4.8472232047529005
3	9	5.016706034951014
3	10	5.082290630060655
3	11	4.609012133083766
3	12	5.005603171882363
3	13	5.927072275880139
3	14	5.358233534293795
3	15	0.1923480248943008
3	16	6.8891354069215005
3	17	7.772035677928304
3	18	8.521562180131866
3	19	7.1600495759021
3	20	6.395200265029203
3	21	7.544426819977858
3	22	8.283572033484553
3	23	6.814481337412651
3	24	8.076001173911877
3	25	7.136460907567326
3	26	6.207569459972959
3	27	6.155835220823995
3	28	5.485795058671844
3	29	6.104837029163786
3	30	5.967841144647771
3	31	5.102349395697118
3	32	5.140618369197362
3	33	5.175380946281596
3	34	4.980291020245723
3	35	5.128396161570503
3	36	6.61260958704402
3	37	7.115509163749287
3	38	7.412031435962909
3	39	8.40150084337344
3	40	9.240471463159185
3	41	11.440064935223617
3	42	10.659911328401346
3	43	10.537582971920909
3	44	11.446164500100775
3	45	10.499202440224192
3	46	12.225181470522092
3	47	12.493925244472479
3	48	12.12597460562202
3	49	12.404597809025478
3	50	12.150251675667377
3	51	11.715760060262312
3	52	11.685993670879366
3	53	11.75211648406733
3	54	12.084734676242316
3	55	11.811803970853866
3	56	11.735373228212062
4	1	3.6038627424537566
4	2	5.112982018610644
4	3	4.298461707426789
4	4	5.441529757998829
4	5	5.13936107009966
4	6	5.105565075150864
4	7	4.530842821140212
4	8	4.672040723001082
4	9	4.56780092546196
4	10	4.881560703053353
4	11	4.992223811915885
4	12	6.009252806918859
4	13	6.416123370770645
4	14	5.953124045242696
4	15	6.641253134383351
4	16	8.159208399622234
4	17	8.914371444351662
4	18	9.444919844365444
4	19	8.032312349624725
4	20	7.3404557394817616
4	21	7.5663246301968385
4	22	8.039046486039297
4	23	7.256087176828714
4	24	8.013957378291444
4	25	7.384636090049078
4	26	6.328007416177343
4	27	6.122988505495535
4	28	6.1098826499127235
4	29	6.426004912375465
4	30	6.3729506336988635
4	31	5.3578238482518685
4	32	5.370545376496636
4	33	5.332315252850936
4	34	4.9036486844793
4	35	5.672191782008458
4	36	6.046916156387084
4	37	6.2541952951361495
4	38	6.631009538152689
4	39	6.799311062351542
4	40	1.587186791626337
4	41	10.96926201551559
4	42	10.316845634970694
4	43	10.336853044913607
4	44	10.942514865064272
4	45	9.240078352632953
4	46	12.287225266142531
4	47	13.012173419654957
4	48	12.764660737008882
4	49	12.240364232383147
4	50	12.438572843550588
4	51	12.171964439824357
4	52	11.547307539492504
4	53	11.982043491366596
4	54	12.135829566753262
4	55	11.73516163508744
4	56	10.520044761058777
5	1	3.9916434300338537
5	2	5.486359184710828
5	3	4.75775852583655
5	4	5.775736032027915
5	5	5.43470290996148
5	6	5.868199498731407
5	7	4.943920215690831
5	8	5.172402900523785
5	9	5.321018733452156
5	10	5.598950796105115
5	11	5.741401947537433
5	12	6.595423197513384
5	13	6.443593142877408
5	14	6.040380968405344
5	15	7.471085418330528
5	16	8.208659561376283
5	17	9.36940571191727
5	18	9.773442717268992
5	19	8.526991126021898
5	20	7.630504215550182
5	21	8.009629917988669
5	22	8.351800706800425
5	23	8.02108968421156
5	24	8.130745699459325
5	25	7.648635644292128
5	26	6.518039176360105
5	27	6.5033584837648775
5	28	6.609381173342822
5	29	6.613445710280406
5	30	6.826118294032064
5	31	5.273659363936936
5	32	5.872607002395114
5	33	5.926732147039385
5	34	5.299926613938261
5	35	5.6821377339781485
5	36	6.752939447187778
5	37	6.9298235338389915
5	38	7.266185333550247
5	39	8.712472036330482
5	40	9.659120819597586
5	41	12.302688214880384
5	42	11.25416106373316
5	43	11.573689284953726
5	44	11.732229023419432
5	45	11.152821429470304
5	46	12.330240811916195
5	47	13.098009492536221
5	48	12.62207423230057
5	49	13.441344897675396
5	50	12.663596524388609
5	51	11.89651450390192
5	52	11.681842559272969
5	53	12.110310054023309
5	54	12.361271144732871
5	55	12.333423182978457
5	56	11.83240894448776
6	1	4.089698682669258
6	2	5.242659923835994
6	3	4.599846251512062
6	4	5.684375314713002
6	5	5.1593182013706596
6	6	5.748364287293143
6	7	4.79373240519768
6	8	4.874450640739461
6	9	4.912227990691946
6	10	5.115369068248576
6	11	5.337548803226277
6	12	6.294532612271652
6	13	6.570334933741332
6	14	6.17499868317573
6	15	6.939914934869972
6	16	7.797778669579362
6	17	8.955647078692987
6	18	9.696773627241122
6	19	8.12071462050875
6	20	7.6426513135751435
6	21	7.804159180075182
6	22	8.250261872437164
6	23	7.732103860874595
6	24	8.499793318506946
6	25	7.402391854155205
6	26	6.036123509329933
6	27	5.806339218043327
6	28	5.874799808990463
6	29	6.270055427958827
6	30	6.697044018011226
6	31	5.195415089127778
6	32	5.698549084062385
6	33	5.508974967831822
6	34	5.834913891291947
6	35	5.712975005273432
6	36	6.993185418349775
6	37	6.8720263691716
6	38	7.382052291020258
6	39	7.369899638414573
6	40	4.588055752349312
6	41	12.165647138908014
6	42	11.125934964133013
6	43	11.364341169117065
6	44	12.101216058437
6	45	11.24042593692214
6	46	12.990909659607947
6	47	13.931736622064875
6	48	13.020656565997413
6	49	13.832445103661097
6	50	14.017619303983906
6	51	13.352621374130926
6	52	13.193292940952357
6	53	12.995048936853792
6	54	13.086704320547728
6	55	12.847721014070181
6	56	12.704061676827518
7	1	4.026852614009236
7	2	5.375642574260503
7	3	4.8092408620694655
7	4	5.726088123867669
7	5	5.282149504434521
7	6	5.908201865735371
7	7	4.889893350195834
7	8	5.061420002127427
7	9	5.041150102172196
7	10	5.315442896229385
7	11	5.338656222938347
7	12	5.920501604519714
7	13	6.25435117589711
7	14	6.063518432160592
7	15	7.36433353952113
7	16	7.8295247013253935
7	17	8.787596137386235
7	18	9.706278979769728
7	19	7.712445886658621
7	20	7.091064011987839
7	21	7.527027397129444
7	22	8.094300262987183
7	23	7.039966540830299
7	24	8.084234071552352
7	25	6.969483031711498
7	26	6.75843801652816
7	27	6.351189716382194
7	28	5.5239323302160095
7	29	6.496338189125314
7	30	6.521425708671984
7	31	5.414407337189793
7	32	5.554215381589152
7	33	5.319329342139685
7	34	5.674061178113654
7	35	5.654650900437698
7	36	7.302616942897579
7	37	7.6522035563255315
7	38	7.978120951042406
7	39	8.860025145981936
7	40	8.861588421230817
7	41	11.065425654965601
7	42	10.731970401563796
7	43	10.974068005588084
7	44	11.34383326035653
7	45	10.439761485094895
7	46	11.97208352450274
7	47	12.694287378801683
7	48	11.804156012287557
7	49	12.003449164200038
7	50	12.209664339052203
7	51	11.470838428394494
7	52	11.662285189014373
7	53	11.425742919873358
7	54	11.205936509547357
7	55	11.332032568149174
7	56	8.948155438363138
8	1	4.1434152912639135
8	2	5.530019583146874
8	3	4.823326700602266
8	4	5.669083018932895
8	5	5.558950411978479
8	6	5.926258209089504
8	7	4.9083278368662775
8	8	5.092994969039893
8	9	5.293277158326482
8	10	5.710413590122371
8	11	5.751021509381907
8	12	6.792500091946451
8	13	7.048073996846252
8	14	6.568339759443639
8	15	7.815931195032572
8	16	8.451475920837591
8	17	9.131895626892753
8	18	9.853471265646078
8	19	8.24402085357108
8	20	7.72521035345126
8	21	7.89562259470933
8	22	8.759359820559503
8	23	7.880768204660889
8	24	8.508794479947127
8	25	7.3523531394823465
8	26	6.937110733487884
8	27	7.011333410842397
8	28	6.2346526932335955
8	29	6.662815784133816
8	30	6.877164033497095
8	31	5.5998866338432265
8	32	6.305208007794484
8	33	5.549335014289429
8	34	6.040198444137481
8	35	5.839184838800339
8	36	6.465407640571996
8	37	7.086312083457315
8	38	7.594305484980772
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115	29	3.9828317450296247
115	30	5.1965814608002034
115	31	3.1632065001707232
115	32	3.8613205321099073
115	33	3.3327406704318783
115	34	3.5010693105148505
115	35	3.787860583226493
115	36	5.703648949365451
115	37	5.242753801248725
115	38	6.2359085574532145
115	39	6.089468242952499
115	40	7.785543336339966
115	41	8.730356458218559
115	42	7.851102945435965
115	43	6.1173599635469
115	44	-1.4232994737472424
115	45	6.129850418371163
115	46	1.276477874624014
115	47	-8.053631962996958
115	48	0.5771859262077506
115	49	-3.596588882121356
115	50	1.773510822145365
115	51	2.8878974477505572
115	52	2.9724340452468354
115	53	3.2388165870631624
115	54	3.131888856529809
115	55	3.3387620112461462
115	56	6.455717144740653
116	1	3.7526707680551326
116	2	4.844329327229531
116	3	4.306605966892469
116	4	5.002656650235714
116	5	5.050259635415543
116	6	5.081150275251545
116	7	4.379391956374469
116	8	4.482277679600983
116	9	4.558506042142412
116	10	4.794077333163742
116	11	3.757910051173731
116	12	3.5527427928956286
116	13	4.7260546929093685
116	14	4.735206674418201
116	15	-3.4833198762958766
116	16	4.746601877023263
116	17	6.369029673110067
116	18	5.536538268729899
116	19	5.7892969959876766
116	20	5.399766803449253
116	21	5.84069448385187
116	22	7.333732042114235
116	23	6.475946225406609
116	24	6.771139788129275
116	25	6.404718071998666
116	26	5.284481473205133
116	27	5.174155309997346
116	28	4.404082786549306
116	29	5.0043192484295425
116	30	4.779282660594696
116	31	3.869060409324824
116	32	4.195812357187727
116	33	4.100518481789127
116	34	3.864060032507055
116	35	3.6286838175339717
116	36	6.013026688191047
116	37	5.655277600698696
116	38	6.267520271315824
116	39	7.2137693702153385
116	40	8.547004849229442
116	41	7.449719388497503
116	42	4.852946458385887
116	43	1.1384243656140534
116	44	0.03621879516387594
116	45	5.083611487004241
116	46	-0.6191067607533007
116	47	-5.336052102620398
116	48	-0.6246636310469249
116	49	-2.4621635443958283
116	50	-0.32625042781685565
116	51	2.319370142603834
116	52	2.641568803021322
116	53	2.9089788766458042
116	54	2.535527527991622
116	55	3.0745956719793206
116	56	5.590898020560413
117	1	2.8333271199596695
117	2	3.8177214038270506
117	3	3.4089788247291466
117	4	4.013237375969425
117	5	4.0208764396894665
117	6	4.428891897398216
117	7	3.7244276880056333
117	8	3.6934759034267017
117	9	3.6014186904460246
117	10	3.3359145634419605
117	11	-0.4716396608917677
117	12	-6.19255658960628
117	13	-8.526356324921753
117	14	-2.1843798100055807
117	15	-7.650460651984492
117	16	-8.266719430130003
117	17	-0.1461302519575831
117	18	-2.7959046647329444
117	19	-0.6531507942017356
117	20	-0.6671519504534729
117	21	4.6128446260845735
117	22	5.738748346839131
117	23	4.726067643570772
117	24	5.257506262839605
117	25	4.735669296587581
117	26	4.452454830590831
117	27	4.63162426163823
117	28	4.360268174740522
117	29	4.420401951544786
117	30	4.866113993419226
117	31	3.43837283560665
117	32	3.905345417619287
117	33	3.762663767295269
117	34	3.354866942935139
117	35	2.543066666351013
117	36	4.995924072496884
117	37	4.424340252471403
117	38	5.212535188404566
117	39	6.255155618740972
117	40	8.237284457916044
117	41	5.891334596989836
117	42	4.6502475060511905
117	43	-5.166231153708782
117	44	-2.226807639305008
117	45	4.349413193796721
117	46	-2.649600065408819
117	47	-2.196421213839436
117	48	-0.9179752247470567
117	49	-1.8002957861220494
117	50	0.08306754900961977
117	51	3.722339683990082
117	52	4.496109842360809
117	53	4.212073305286612
117	54	4.507115677578799
117	55	4.243540374678272
117	56	6.662534737413253
118	1	2.5688653493359226
118	2	3.7257948444709115
118	3	3.0353621245279356
118	4	4.0226689655066465
118	5	3.9351118521643116
118	6	3.8326896841185345
118	7	3.4018673258325975
118	8	3.45579984708867
118	9	3.4439740224379776
118	10	3.126281766661279
118	11	-1.3439988158213438
118	12	-7.622134054395012
118	13	-7.1270857011793005
118	14	-10.71984258666554
118	15	-8.759489827034791
118	16	-11.185356251055556
118	17	-1.800305302259396
118	18	-8.751639071171578
118	19	-1.7205552207610921
118	20	-1.4863169403931096
118	21	2.369635370551375
118	22	3.8712936184689113
118	23	2.964246718017449
118	24	3.246817128030756
118	25	2.659336298599655
118	26	3.6373139855204073
118	27	3.0950296942338014
118	28	3.0851172693079256
118	29	3.58652368192708
118	30	3.291290049757258
118	31	1.6150579462706318
118	32	2.7392236872369935
118	33	2.291713063069917
118	34	2.3289615103395676
118	35	3.0443242116226408
118	36	3.441598116762478
118	37	3.407740654885888
118	38	3.685623719591689
118	39	4.007796463811395
118	40	6.3142462285397905
118	41	4.516837615098488
118	42	4.060458773656823
118	43	-1.3668096245337296
118	44	-1.3948156875947433
118	45	4.439830698826903
118	46	-3.0507570070587207
118	47	-0.8351284573002005
118	48	-0.586486291145448
118	49	-1.2250945788785863
118	50	0.0850796214442262
118	51	5.022264231273784
118	52	5.01472151238093
118	53	3.8095330638379217
118	54	5.1124979713413765
118	55	4.673117839467004
118	56	6.759617232383071
119	1	2.720651063621638
119	2	4.134337315513385
119	3	3.545255946921756
119	4	4.329377459715147
119	5	4.355478647531108
119	6	4.585344124272979
119	7	3.8881124995777725
119	8	3.8037728200616456
119	9	3.953626532090489
119	10	4.425750878630389
119	11	0.28606875174622104
119	12	-6.6559178381787945
119	13	-3.0259756625692624
119	14	-7.587120578943534
119	15	-7.548823803868771
119	16	-9.039844668043973
119	17	0.3555827286286366
119	18	-6.732334051866559
119	19	-0.5699760701819443
119	20	-0.690467519543688
119	21	4.596951972867977
119	22	5.501119873295167
119	23	4.7359648647356
119	24	3.9753402940539218
119	25	4.420195371958727
119	26	5.890692363898786
119	27	5.446622358326465
119	28	5.418128852319509
119	29	5.822286230189629
119	30	5.9720332930005
119	31	4.972442116154802
119	32	4.658866544379848
119	33	5.117726576583433
119	34	5.242571548949606
119	35	5.2658493081477395
119	36	5.710903136067493
119	37	6.511987759132992
119	38	6.853094762062732
119	39	7.100701869216799
119	40	8.552663216956777
119	41	4.4304476537085264
119	42	3.88140471960277
119	43	-4.75749495271906
119	44	-0.25002804280709867
119	45	2.8343770308732346
119	46	-2.3794249607266735
119	47	-3.645697955369698
119	48	-5.433011387670543
119	49	-4.010326239110249
119	50	-1.186155899791295
119	51	3.8458646173741684
119	52	3.760860508519926
119	53	4.055430747235604
119	54	2.427652411495817
119	55	3.8825772989264635
119	56	6.25865198141782
120	1	2.834444167069911
120	2	4.28275297254973
120	3	3.3923202526067513
120	4	4.39181921181207
120	5	4.326121704381059
120	6	4.784598551113611
120	7	3.9734806263252125
120	8	3.925627433295567
120	9	3.9328902785956146
120	10	4.040382751882952
120	11	-1.0539126089247937
120	12	-6.342823709567426
120	13	-5.353748262755655
120	14	-5.424399236911846
120	15	-0.3020390881185371
120	16	-9.53714196534127
120	17	0.7512956829622794
120	18	-4.056565179545963
120	19	-0.6658754178054274
120	20	-0.8031888615753751
120	21	3.4633545823740377
120	22	5.065751746547733
120	23	4.2897393288548855
120	24	5.089366389114495
120	25	4.01949639712182
120	26	5.610589847589374
120	27	5.306175014430842
120	28	5.524279830884279
120	29	5.642742893749745
120	30	5.747755566998634
120	31	4.8040357787829535
120	32	4.224383785759159
120	33	5.143868235483712
120	34	4.71824722462528
120	35	4.7567626352679575
120	36	5.478051318732916
120	37	5.6345879455262775
120	38	6.180820763926661
120	39	7.548744739673467
120	40	7.908581203909247
120	41	-4.899049084408901
120	42	-2.631782605653523
120	43	-5.078324402858854
120	44	-14.12141667281642
120	45	-4.816881123833195
120	46	-8.39447621888138
120	47	-6.8463969302066054
120	48	-5.804959197549389
120	49	-13.631621672474648
120	50	-4.812949935206019
120	51	-0.683585522420799
120	52	-0.8963622314614348
120	53	-0.5678684139945922
120	54	-0.28516212718079004
120	55	0.19380749463941882
120	56	2.997794572284551
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	6209.0
5	613.0
6	364.0
7	468.0
8	901.0
9	867.0
10	625.0
11	508.0
12	419.0
13	437.0
14	593.0
15	675.0
16	824.0
17	1043.0
18	1366.0
19	1665.0
20	2117.0
21	2609.0
22	3143.0
23	3676.0
24	4178.0
25	4517.0
26	4924.0
27	5312.0
28	5479.0
29	5963.0
30	6744.0
31	7491.0
32	8568.0
33	9557.0
34	10268.0
35	11592.0
36	12360.0
37	13320.0
38	12798.0
39	10152.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.42161639431102	0.5174038645141024	0.038805289838557674	0.022174451336318672
8	99.41792065242163	0.489685800343704	0.05235634343297464	0.04003720380168649
9	97.13946053822984	1.2602635096429253	1.0348204771261558	0.565455475001078
10	53.54329253644925	23.038638981453534	15.280044841668259	8.138023640428953
11	35.305422269308714	28.091334101226373	18.49041262958195	18.112830999882966
12	28.978312154679113	17.318246493664883	20.492272819666272	33.21116853198973
13	22.762074296731114	14.460822056187597	23.97304572248674	38.804057924594545
14	20.58774615180876	23.05526981995577	32.27676179095652	24.080222237278946
15	32.41416448480122	19.06857556682079	23.16057910732302	25.356680841054967
16	35.332367715492005	22.005608801503897	21.37076643348023	21.291257049523868
17	26.297597042513864	20.059765865680838	33.13185459026494	20.510782501540355
18	24.083917979168334	19.88247540791751	29.890544444376005	26.14306216853815
19	32.21701891675396	22.89913118491589	19.924825928892723	24.95902396943743
20	26.603761948812828	28.245451742214	24.209065679925995	20.94172062904718
21	24.580682119164507	20.899677850530036	29.537964976254568	24.981675054050893
22	21.636720891412946	19.413732144946998	27.862814061065976	31.08673290257408
23	21.99767168260969	20.936377803374214	25.653992325176013	31.41195818884009
24	22.511395835899965	21.337932733768632	29.536158679315015	26.614512751016385
25	30.753925476015944	23.243622833981163	22.933157567282876	23.06929412272002
26	26.683881941833736	21.847786563457188	21.80834345090257	29.659988043806507
27	22.060518643169306	23.333394973833606	20.96208492828128	33.644001454715806
28	24.049104695497913	29.46897694444616	21.78789876006332	24.694019599992608
29	34.44351226974942	23.36249120945554	19.202867259694273	22.991129261100763
30	26.967379313320063	20.475289506283165	31.008449454267563	21.548881726129213
31	28.06530109348529	28.608039427075312	21.814261512397966	21.51239796704143
32	35.36025629177833	22.675045436342913	20.984505436959	20.980192834919755
33	28.21709824429411	22.459098281295287	21.51371818669561	27.81008528771499
34	24.063662900130215	28.385408631148906	27.001191056584446	20.549737412136434
35	23.49450725683586	24.78488554986029	32.54936067060195	19.1712465227019
36	25.77953609899184	30.261406492642166	23.20860755749464	20.750449850871355
37	26.47227551152591	22.44175359163053	29.832277485457677	21.253693411385886
38	32.87243367365835	24.932408241873556	21.437389661856027	20.757768422612067
39	26.81329254453052	23.182390092268218	20.607391325800563	29.3969260374007
40	21.517005746233885	18.719676968473365	26.742661904022363	33.02065538127039
41	19.00024281062639	16.50863222906381	23.45177096109427	41.039353999215535
42	28.053096798502715	24.043040773844453	23.02693754516872	24.87692488248411
43	19.62767534971084	19.932900004942912	30.658395531609905	29.781029113736345
44	21.790714082362697	22.103875527608956	23.697533079998514	32.40787731002983
45	24.312362657712157	22.121676007999802	30.218389669390877	23.347571664897163
46	21.333489889751323	24.98393236762743	24.21206802788352	29.470509714737723
47	18.519046056094677	25.80553267648024	24.10002662917939	31.575394638245697
48	23.94343623631522	27.008950586842882	25.183080185422625	23.86453299141927
49	21.50256026980117	26.82260943686418	23.34200139594928	28.332828897385376
50	27.909775364107624	22.645025919526045	25.402986916810665	24.042211799555666
51	21.482021590157753	27.659995180216647	27.44804829670092	23.40993493292468
52	22.322249440046402	23.78460760056026	30.752713383970825	23.140429575422512
53	22.007373875236997	22.423621103858007	31.282152628101013	24.286852392803986
54	22.62137780714158	27.3437355282088	26.52683185862565	23.50805480602397
55	21.64704574372151	27.584588207681186	25.865647119862633	24.902718928734664
56	27.269135070724282	21.434613223681367	27.489550340501463	23.806701365092888
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	15.5
7	30.0
8	89.5
9	149.0
10	195.0
11	241.0
12	268.5
13	277.5
14	259.0
15	268.5
16	278.0
17	281.0
18	284.0
19	294.0
20	304.0
21	306.0
22	342.5
23	377.0
24	417.5
25	458.0
26	529.0
27	600.0
28	694.5
29	900.0
30	1011.0
31	1200.5
32	1390.0
33	1700.5
34	2011.0
35	2194.5
36	2378.0
37	2491.0
38	2897.5
39	3191.0
40	3699.5
41	4208.0
42	5407.0
43	6606.0
44	10244.0
45	13882.0
46	12933.0
47	10547.5
48	9111.0
49	9026.5
50	8942.0
51	9301.5
52	9661.0
53	9990.5
54	10194.0
55	10068.0
56	10310.0
57	10552.0
58	10830.5
59	11109.0
60	10366.5
61	9624.0
62	8575.5
63	7047.0
64	6567.0
65	5441.0
66	4315.0
67	3910.0
68	3505.0
69	3079.0
70	2653.0
71	2348.0
72	1536.5
73	1030.0
74	793.5
75	557.0
76	489.0
77	421.0
78	473.5
79	333.0
80	140.0
81	86.5
82	33.0
83	18.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	1.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.001231913963128815
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.48660601543588194
16	0.06405952608269838
17	0.030181892096655973
18	0.0
19	0.03634146191230004
20	0.1225754393313171
21	0.001231913963128815
22	0.0
23	0.0
24	0.005543612834079668
25	0.007391483778772891
26	0.05543612834079668
27	0.07268292382460008
28	0.001231913963128815
29	0.14967754652015103
30	0.05543612834079668
31	0.014782967557545781
32	0.020942537373189858
33	0.11826374046036625
34	0.18971475032183752
35	0.1392062778335561
36	0.04496485965420175
37	0.14536584764920016
38	0.21373707260284944
39	0.1311988370732188
40	0.846324892669496
41	1.065605578106425
42	0.2808763835933698
43	0.30859444776376815
44	0.47490283278615825
45	0.2125051586397206
46	0.3283050711738292
47	0.5371144879241634
48	0.07699462269555093
49	0.2784125556671122
50	0.19033070730340193
51	0.3178338024872343
52	0.17369986880116292
53	0.2623976741464376
54	0.2439189646995054
55	0.2753327707592902
56	0.23529556695760367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	162349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.11637300188298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.73799708903223	58.009181419765774
2	7.388183085599937	9.76959737387456
3	2.0259919976804373	4.018537278524096
4	0.8866537445959132	2.3448931880487867
5	0.4887949945287356	1.6158676089857615
6	0.2978744253986168	1.1816625970425907
7	0.19091948347743154	0.8836032648044488
8	0.12494730443519637	0.6608850068493817
9	0.10695489250024276	0.6364322610230111
>10	0.6537242961693942	8.175808411782384
>50	0.05297765702897987	2.5212762986773734
>100	0.03898355894585311	4.791416294996319
>500	0.003998313738036216	1.4922783440140257
>1k	0.001999156869018108	3.8985606516114872
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	4673	2.878366974850476	No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	1226	0.7551632593979636	No Hit
ATACAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	600	0.3695741889386445	No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	580	0.35725504930735635	No Hit
ATACAGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	540	0.3326167700447801	No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	538	0.33138485608165125	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	474	0.2919636092615292	No Hit
ATACAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	451	0.2777965986855478	No Hit
ATACAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	412	0.2537742764045359	No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG	318	0.1958743201374816	No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	317	0.1952583631559172	No Hit
ATACAGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	291	0.1792434816352426	No Hit
ATACAGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	270	0.16630838502239004	No Hit
ATACAGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	251	0.1546052023726663	No Hit
ATACAGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	245	0.15090946048327986	No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC	226	0.1392062778335561	No Hit
ATACAGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	215	0.1324307510363476	No Hit
ATACAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	204	0.12565522423913913	No Hit
ATACAGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	202	0.12442331027601031	No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	175	0.10779247177377133	No Hit
ATACAGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT	170	0.10471268686594928	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	166	0.10224885893969164	No Hit
ATACAGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	165	0.10163290195812724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	6.159569815644075E-4	0.0	0.0	0.0	0.0
11	0.0018478709446932227	0.0	0.0	0.0	0.0
12	0.0030797849078220375	0.0	0.0	0.0	0.0
13	0.0030797849078220375	0.0	0.0	0.0	0.0
14	0.005543612834079668	0.0	0.0	0.0	0.0
15	0.007391483778772891	0.0	0.0	0.0	0.0
16	0.008007440760337298	0.0	0.0	0.0	0.0
17	0.008007440760337298	0.0	0.0	0.0	0.0
18	0.008623397741901706	0.0	0.0	0.0	0.0
19	0.008623397741901706	0.0	0.0	0.0	0.0
20	0.009239354723466113	0.0	0.0	0.0	0.0
21	0.010471268686594929	0.0	0.0	0.0	0.0
22	0.011087225668159336	0.0	0.0	0.0	0.0
23	0.01231913963128815	0.0	0.0	0.0	0.0
24	0.015398924539110188	0.0	0.0	0.0	0.0
25	0.015398924539110188	0.0	0.0	0.0	0.0
26	0.016630838502239002	0.0	0.0	0.0	0.0
27	0.01971062341006104	0.0	0.0	0.0	0.0
28	0.025870193225705117	0.0	0.0	0.0	0.0
29	0.025870193225705117	0.0	0.0	0.0	0.0
30	0.02710210718883393	0.0	0.0	0.0	0.0
31	0.028334021151962745	0.0	0.0	0.0	6.159569815644075E-4
32	0.030797849078220377	0.0	0.0	0.0	6.159569815644075E-4
33	0.030797849078220377	0.0	0.0	0.0	6.159569815644075E-4
34	0.0326457200229136	0.0	0.0	0.0	6.159569815644075E-4
35	0.03387763398604241	0.0	0.0	0.0	6.159569815644075E-4
36	0.03572550493073564	0.0	0.0	0.0	6.159569815644075E-4
37	0.036957418893864454	0.0	0.0	0.0	6.159569815644075E-4
38	0.03757337587542886	0.0	0.0	0.0	6.159569815644075E-4
39	0.03818933285699327	0.0	0.0	0.0	6.159569815644075E-4
40	0.040653160783250895	0.0	0.0	0.0	6.159569815644075E-4
41	0.0412691177648153	0.0	0.0	0.0	6.159569815644075E-4
42	0.04496485965420175	0.0	0.0	0.0	6.159569815644075E-4
43	0.046196773617330565	0.0	0.0	0.0	6.159569815644075E-4
44	0.053588257396103456	0.0	0.0	0.0	6.159569815644075E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCATAT	15	0.008095348	50.496555	40
GCCCTAA	15	0.008115412	50.46496	39
CTAGACC	20	4.036227E-4	50.33895	36
CAATTCG	15	0.008226425	50.291862	42
TCTAGAC	20	4.0549194E-4	50.29186	35
ACCCGAT	20	4.0549194E-4	50.29186	42
ATTATAC	20	4.0549194E-4	50.29186	42
CACGATT	135	0.0	50.260517	47
CTCCACG	125	0.0	50.24486	44
ATTCGCC	15	0.008256897	50.244858	44
AATTCGC	15	0.008256897	50.244858	43
TCGTTGA	35	5.102811E-8	50.182327	14
ACCTAAA	15	0.008297657	50.182327	15
GTATGAT	20	4.098803E-4	50.182327	15
TCGCGCA	20	4.1621475E-4	50.026676	50
ACTAATC	30	1.0406275E-6	50.011166	10
GTACCTC	35	5.3067197E-8	49.933746	9
CATATAC	20	4.2133807E-4	49.902843	33
AGGTAAG	15	0.0085141035	49.85657	31
ACCGACG	15	0.0085141035	49.85657	31
>>END_MODULE