FastQCFastQC Report
Fri 17 Jun 2016
SRR1526101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGTGAGATGGACTTAAAGGAATTGACGGGTACG110.5099675475197033No Hit
ATACAGGGGTGAGATGGACTTAAATGAATTGACGGGTACG100.4636068613815485No Hit
ATACAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT80.3708854891052387No Hit
ATACAGGGGTGAGATGGACTCAAAGGAATTGACGGGTACG70.3245248029670839No Hit
ATACAGGGGTGAGATGGACTTAAAGGAATTGACGGGTCCG50.23180343069077425No Hit
ATACAGGGGCTCGTCTTAATCCGCAACATCTTTTGATACA40.18544274455261936No Hit
ATACAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.18544274455261936No Hit
ATACAGGGGTGAGATGGACTCAAATGAATTGACGGGTACG40.18544274455261936No Hit
ATACAGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC40.18544274455261936No Hit
ATACAGGGGATGAGATGGACTTAAATGAATTGACGGGTAC40.18544274455261936No Hit
ATACAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT30.13908205841446453No Hit
ATACAGGGGTCCAGAGCGATTTTTTTTTTTTTTTTTTTTT30.13908205841446453No Hit
ATACAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.13908205841446453No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG30.13908205841446453No Hit
ATACAGGGGTTCCGCAATCGATTTTTTTTTTTTTTTTTTT30.13908205841446453No Hit
ATACAGGGGTGTTCTCTATTTTAATAATGGGAAGTATTTT30.13908205841446453No Hit
ATACAGGGACCAACAGGTCCCTCTGTATCCTGCTGCCTAA30.13908205841446453No Hit
ATACAGGGACCAACAGGTCCCTCTGTATCCTGCTGCCTCA30.13908205841446453No Hit
ATACAGGGATGAGATGGACTCAAATGAATTGCCGGGTACG30.13908205841446453No Hit
ATACAGGGGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTT30.13908205841446453No Hit
ATACAGGGATGAGATGGACTTAAATGAATTGACGGGTACG30.13908205841446453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGAC200.002058959834.4761913
GGACTTA200.002058959834.4761916
ATGGACT200.002058959834.4761914
ACTTAAA200.002058959834.4761918
TGGACTT200.002058959834.4761915
GAATTGA200.002058959834.4761926
GACTTAA200.002058959834.4761917
AGGGGTG251.5175008E-433.674425
AGGGGAG251.5175008E-433.674425
AGGGGAC200.002324525333.674425
TACAGGG1850.033.674422
TACAGGA200.002324525333.674422
GACGGGT200.002324525333.6744231
GTGAGAT200.002324525333.674429
CGGGTAC200.002324525333.6744233
CAGGGGT309.717363E-633.674424
CAGGGGG501.3096724E-1033.674424
CAGGGGC356.0960883E-733.674424
CAGGGGA600.033.674424
ACGGGTA200.002324525333.6744232
Produced by FastQC (version 0.11.5)