FastQCFastQC Report
Fri 17 Jun 2016
SRR1526099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1853.801109513047052No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC531.0889665091432095No Hit
ATACAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT250.513663447709061No Hit
ATACAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT180.36983768235052394No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG140.28765153071707417No Hit
ATACAGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT130.2671049928087117No Hit
ATACAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.2465584549003493No Hit
ATACAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.22601191699198686No Hit
ATACAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA90.18491884117526197No Hit
ATACAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.16437230326689953No Hit
ATACAGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG80.16437230326689953No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG70.14382576535853708No Hit
ATACAGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT70.14382576535853708No Hit
ATACAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG70.14382576535853708No Hit
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG60.12327922745017465No Hit
ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC60.12327922745017465No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAG60.12327922745017465No Hit
ATACAGGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA60.12327922745017465No Hit
ATACAGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT60.12327922745017465No Hit
ATACAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG50.10273268954181221No Hit
ATACAGGGGGGGCTAGAGAGATGGCTCAGAGGTTAAGAGC50.10273268954181221No Hit
ATACAGGGGAAGGAAAAAAATCTATCTGAAAGGATTTTTG50.10273268954181221No Hit
ATACAGGGGGATGGTAGTCGCCGTGCCTACCATGGTGACC50.10273268954181221No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG50.10273268954181221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA251.6824547E-434.2083365
AGGGGAT502.382876E-1034.2083365
AGGGGAG357.836479E-734.2083365
AGGGGAC251.6824547E-434.2083365
TAGCATA251.6824547E-434.20833630
CAGGGGA1400.034.2083364
AGCATAT251.6824547E-434.20833631
AGGGGGT405.3032636E-834.2083325
AGGGGGG1300.034.2083325
AGGGGGA650.034.2083325
AGGGGAA301.1511505E-534.2083325
GTACCTG200.002448129534.2083329
GGGTACC200.002448129534.2083327
CAGGGGT453.5652192E-934.2083324
CAGGGGG2500.034.2083324
CAGGGGC200.002448129534.2083324
GGGGTAC200.002448129534.2083326
ACAGGGG4550.034.2083323
GGTACCT200.002448129534.2083328
ATCCTGC200.00257856733.8556720
Produced by FastQC (version 0.11.5)