##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526099_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4867 Sequences flagged as poor quality 0 Sequence length 40 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.105198274090817 25.0 4.0 30.0 4.0 33.0 2 14.239983562769673 11.0 4.0 25.0 4.0 31.0 3 14.201561536881036 10.0 4.0 25.0 4.0 31.0 4 11.86624203821656 5.0 4.0 19.0 4.0 27.0 5 12.788576124922951 6.0 4.0 22.0 4.0 31.0 6 11.541606739264434 4.0 4.0 19.0 4.0 28.0 7 15.337785083213479 4.0 4.0 29.0 4.0 33.0 8 15.058146702280666 4.0 4.0 28.0 4.0 33.0 9 14.902403944935278 4.0 4.0 28.0 4.0 33.0 10 13.315389356893364 4.0 4.0 24.0 4.0 32.0 11 13.010478734333265 4.0 4.0 25.0 4.0 32.0 12 12.703924388740496 4.0 4.0 24.0 4.0 31.0 13 12.073556605711937 4.0 4.0 21.0 4.0 31.0 14 12.60324635298952 4.0 4.0 24.0 4.0 33.0 15 12.392438874049722 4.0 4.0 24.0 4.0 33.0 16 10.594411341688925 4.0 4.0 19.0 4.0 29.0 17 10.008835011300595 4.0 4.0 15.0 4.0 28.0 18 9.403123073762071 4.0 4.0 13.0 4.0 26.0 19 9.63550441750565 4.0 4.0 13.0 4.0 28.0 20 9.963016231764948 4.0 4.0 15.0 4.0 29.0 21 8.790014382576535 4.0 4.0 10.0 4.0 26.0 22 8.485514690774604 4.0 4.0 10.0 4.0 23.0 23 8.618656256420794 4.0 4.0 10.0 4.0 24.0 24 8.428395315389357 4.0 4.0 9.0 4.0 23.0 25 8.72221080747894 4.0 4.0 10.0 4.0 25.0 26 9.028148756934456 4.0 4.0 10.0 4.0 27.0 27 9.177933018286419 4.0 4.0 10.0 4.0 27.0 28 9.612903225806452 4.0 4.0 12.0 4.0 29.0 29 8.417300184918842 4.0 4.0 9.0 4.0 24.0 30 8.832956646805012 4.0 4.0 10.0 4.0 26.0 31 9.95294842818985 4.0 4.0 4.0 4.0 31.0 32 9.573659338401479 4.0 4.0 4.0 4.0 30.0 33 9.470926648859667 4.0 4.0 4.0 4.0 30.0 34 9.76309841791658 4.0 4.0 4.0 4.0 31.0 35 9.757756318060407 4.0 4.0 4.0 4.0 31.0 36 9.643517567289912 4.0 4.0 4.0 4.0 32.0 37 9.52044380521882 4.0 4.0 4.0 4.0 32.0 38 9.217998767207726 4.0 4.0 4.0 4.0 31.0 39 8.94719539757551 4.0 4.0 4.0 4.0 30.0 40 9.262584754468872 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 11.40309975127419 1 2 6.819851443913313 1 3 -4.996860332027675 1 4 -0.15529798689027707 1 5 8.514001391891856 1 6 -1.1468952221994826 1 7 -8.416199737269718 1 8 -8.141012418953595 1 9 -6.203045695008169 1 10 -6.757438707337286 1 11 -3.15746815163651 1 12 -3.280902101617115 1 13 -5.579953396839809 1 14 -5.937497517695286 1 15 -5.50250134415956 1 16 -3.279027519696891 1 17 -3.594456473209008 1 18 -0.7787149772697433 1 19 -0.024532617483936647 1 20 -0.534246233257389 1 21 0.9765789737645694 1 22 -2.8890612852326836 1 23 -3.320570239992147 1 24 -2.894183369031241 1 25 -2.8425356365518626 1 26 -3.338595709564271 1 27 -3.199400832996167 1 28 -3.387036563278956 1 29 -2.906735829529948 1 30 -2.6505464278790827 1 31 -3.3856802314205954 1 32 -3.064026873509631 1 33 -3.282040086350431 1 34 -3.081008806946942 1 35 -3.173682124163869 1 36 -3.206814351413744 1 37 -2.8951343450329245 1 38 -3.0701780668058545 1 39 -3.1067183098623277 1 40 -3.1612117457705686 2 1 6.271231619406059 2 2 -2.905423281361413 2 3 4.7613814262140846 2 4 4.668877837285548 2 5 3.53597941386988 2 6 1.0618959865917272 2 7 0.8914925704225904 2 8 0.08975681181563466 2 9 -7.653595145557619 2 10 -1.8673288172273956 2 11 -5.322303316471674 2 12 -0.5776053983204115 2 13 0.06839825151183909 2 14 -1.783651363849133 2 15 0.7282678866096699 2 16 -0.8504560911254622 2 17 -0.8472037259562608 2 18 2.232274033719267 2 19 2.2941487011973827 2 20 3.5976218986107433 2 21 0.9106449078305037 2 22 1.0340156378442398 2 23 2.6025066830847763 2 24 -0.9711064459543177 2 25 1.7728489788327524 2 26 1.5844812135126523 2 27 2.1082914746961414 2 28 1.9206557444133523 2 29 2.7855718627777435 2 30 1.426376649043994 2 31 4.537396691656328 2 32 3.935973126490369 2 33 3.487190682880339 2 34 4.995914269976135 2 35 2.903240952759208 2 36 4.254724110124718 2 37 5.335634885736305 2 38 6.006745010117222 2 39 4.893281690137672 2 40 5.069557484998661 3 1 15.117385465559906 3 2 6.24842287248474 3 3 19.145996810829466 3 4 13.13041629882401 3 5 16.22828710617757 3 6 2.138819063514804 3 7 6.583800262730282 3 8 10.858987581046405 3 9 11.654097162134688 3 10 4.671132721234143 3 11 21.98538899122063 3 12 -6.423759244474258 3 13 -6.008524825411238 3 14 -6.937497517695286 3 15 -6.50250134415956 3 16 -4.850456091125462 3 17 -4.3087421874947225 3 18 -3.9215721201268865 3 19 -4.167389760341079 3 20 -4.248531947543103 3 21 -3.166278169092573 3 22 -2.8890612852326836 3 23 -3.320570239992147 3 24 -2.894183369031241 3 25 -2.8425356365518626 3 26 -3.338595709564271 3 27 -3.199400832996167 3 28 -3.387036563278956 3 29 -2.906735829529948 3 30 -2.6505464278790827 3 31 -3.3856802314205954 3 32 -3.064026873509631 3 33 -3.282040086350431 3 34 -3.081008806946942 3 35 -3.173682124163869 3 36 -3.206814351413744 3 37 -2.8951343450329245 3 38 -3.0701780668058545 3 39 -3.1067183098623277 3 40 -3.1612117457705686 4 1 18.117385465559906 4 2 19.24842287248474 4 3 22.145996810829466 4 4 18.13041629882401 4 5 18.22828710617757 4 6 6.138819063514804 4 7 -2.416199737269718 4 8 18.858987581046406 4 9 -8.345902837865312 4 10 -7.328867278765857 4 11 -7.014611008779367 4 12 -6.423759244474258 4 13 -6.008524825411238 4 14 -6.937497517695286 4 15 -6.50250134415956 4 16 -4.850456091125462 4 17 -4.3087421874947225 4 18 -3.9215721201268865 4 19 -4.167389760341079 4 20 -4.248531947543103 4 21 -3.166278169092573 4 22 -2.8890612852326836 4 23 -3.320570239992147 4 24 -2.894183369031241 4 25 -2.8425356365518626 4 26 -3.338595709564271 4 27 -3.199400832996167 4 28 -3.387036563278956 4 29 -2.906735829529948 4 30 -2.6505464278790827 4 31 -3.3856802314205954 4 32 -3.064026873509631 4 33 -3.282040086350431 4 34 -3.081008806946942 4 35 -3.173682124163869 4 36 -3.206814351413744 4 37 -2.8951343450329245 4 38 -3.0701780668058545 4 39 -3.1067183098623277 4 40 -3.1612117457705686 5 1 10.617385465559906 5 2 9.24842287248474 5 3 9.645996810829468 5 4 9.13041629882401 5 5 3.7282871061775715 5 6 20.138819063514802 5 7 13.583800262730282 5 8 15.858987581046405 5 9 15.154097162134688 5 10 13.171132721234143 5 11 17.48538899122063 5 12 15.576240755525742 5 13 21.991475174588764 5 14 21.562502482304716 5 15 15.49749865584044 5 16 6.149543908874538 5 17 8.691257812505278 5 18 -3.9215721201268865 5 19 -4.167389760341079 5 20 -4.248531947543103 5 21 -3.166278169092573 5 22 -2.8890612852326836 5 23 -3.320570239992147 5 24 -2.894183369031241 5 25 -2.8425356365518626 5 26 -3.338595709564271 5 27 -3.199400832996167 5 28 -3.387036563278956 5 29 -2.906735829529948 5 30 -2.6505464278790827 5 31 -3.3856802314205954 5 32 -3.064026873509631 5 33 -3.282040086350431 5 34 -3.081008806946942 5 35 -3.173682124163869 5 36 -3.206814351413744 5 37 -2.8951343450329245 5 38 -3.0701780668058545 5 39 -3.1067183098623277 5 40 -3.1612117457705686 6 1 7.760242608417048 6 2 6.74842287248474 6 3 2.3602825251151813 6 4 7.0589877273954365 6 5 9.156858534748999 6 6 -4.5754666507709105 6 7 1.7266574055874244 6 8 6.07327329533212 6 9 7.868382876420403 6 10 0.5997041498055715 6 11 0.9853889912206331 6 12 5.647669326954315 6 13 6.205760888874478 6 14 5.705359625161856 6 15 6.211784370126155 6 16 8.006686766017395 6 17 8.619829241076705 6 18 4.935570737015971 6 19 6.404038811087492 6 20 7.108610909599754 6 21 8.04800754519314 6 22 4.968081571910174 6 23 5.965144045722139 6 24 4.605816630968759 6 25 5.371750077733851 6 26 5.375690004721442 6 27 4.514884881289547 6 28 6.684392008149615 6 29 6.378978456184338 6 30 6.778025000692346 6 31 8.685748340007978 6 32 9.150258840776084 6 33 8.28938848507814 6 34 7.990419764481629 6 35 11.326317875836132 6 36 8.864614220014829 6 37 9.747722797824217 6 38 7.286964790337003 6 39 9.464710261566246 6 40 8.838788254229431 7 1 10.200718798893238 7 2 10.915089539151408 7 3 9.645996810829468 7 4 7.547082965490677 7 5 6.2282871061775715 7 6 -0.8611809364851961 7 7 2.583800262730282 7 8 9.275654247713073 7 9 9.904097162134688 7 10 10.421132721234143 7 11 4.652055657887299 7 12 10.076240755525742 7 13 10.241475174588762 7 14 7.145835815638046 7 15 9.914165322507108 7 16 14.149543908874538 7 17 15.857924479171945 7 18 16.161761213206447 7 19 13.249276906325589 7 20 14.751468052456897 7 21 15.41705516424076 7 22 13.527605381433984 7 23 14.679429760007853 7 24 7.105816630968759 7 25 16.49079769678147 7 26 11.244737623769062 7 27 13.633932500337165 7 28 16.52963010338771 7 29 15.426597503803384 7 30 16.68278690545425 7 31 19.947653101912735 7 32 16.935973126490367 7 33 16.21795991364957 7 34 14.585657859719726 7 35 15.159651209169464 7 36 13.209852315252924 7 37 13.104865654967075 7 38 9.429821933194145 7 39 14.976615023471005 7 40 14.922121587562764 8 1 -10.311185963011523 8 2 -8.894434270372404 8 3 -7.996860332027675 8 4 -5.726726558318847 8 5 -6.914570036679572 8 6 -4.861180936485196 8 7 -6.273342594412575 8 8 -6.426726704667881 8 9 -5.060188552151026 8 10 -6.328867278765857 8 11 -6.014611008779367 8 12 -4.852330673045687 8 13 -4.865667682554094 8 14 -5.794640374838143 8 15 -4.931072772730989 8 16 -1.5647418054111766 8 17 -3.594456473209008 8 18 -3.207286405841172 8 19 -3.8816754746267934 8 20 -2.677103376114532 8 21 -2.737706740521144 8 22 -1.8890612852326836 8 23 -1.4634273828492903 8 24 -2.751326226174098 8 25 -0.2711070651232914 8 26 -1.4814528524214143 8 27 0.08631345271811863 8 28 -2.244179420421813 8 29 -1.763878686672805 8 30 0.9208821435494885 8 31 0.1857483400079758 8 32 1.3645445550617978 8 33 1.1465313422209977 8 34 1.347562621624487 8 35 1.25488930440756 8 36 1.221757077157685 8 37 1.5334370835385043 8 38 1.3583933617655743 8 39 1.3218531187091012 8 40 1.2673596828008602 9 1 1.3673854655599058 9 2 3.24842287248474 9 3 8.895996810829468 9 4 7.880416298824009 9 5 6.7282871061775715 9 6 9.888819063514804 9 7 8.583800262730282 9 8 9.608987581046405 9 9 10.654097162134688 9 10 11.171132721234143 9 11 7.485388991220633 9 12 12.326240755525742 9 13 12.741475174588762 9 14 12.312502482304714 9 15 4.24749865584044 9 16 5.399543908874538 9 17 6.1912578125052775 9 18 1.8284278798731135 9 19 8.332610239658921 9 20 8.751468052456897 9 21 2.083721830907427 9 22 8.860938714767316 9 23 2.179429760007853 9 24 5.355816630968759 9 25 7.907464363448137 9 26 8.66140429043573 9 27 7.550599167003833 9 28 9.612963436721044 9 29 4.093264170470052 9 30 5.849453572120917 9 31 10.364319768579405 9 32 7.935973126490369 9 33 9.467959913649569 9 34 8.668991193053058 9 35 10.326317875836132 9 36 7.043185648586256 9 37 11.854865654967075 9 38 4.9298219331941455 9 39 3.1432816901376723 9 40 4.588788254229431 10 1 -1.5492812011067603 10 2 2.915089539151408 10 3 4.812663477496136 10 4 -1.8695837011759906 10 5 0.5616204395109055 10 6 2.138819063514804 10 7 5.917133596063614 10 8 5.525654247713073 10 9 9.320763828801356 10 10 -0.6622006120991912 10 11 2.652055657887299 10 12 4.242907422192408 10 13 2.9914751745887624 10 14 3.3958358156380477 10 15 2.164165322507106 10 16 0.8162105755412039 10 17 -1.6420755208280555 10 18 1.4117612132064474 10 19 2.499276906325587 10 20 -0.5818652808764364 10 21 2.500388497574093 10 22 -0.5557279518993505 10 23 2.346096426674519 10 24 2.772483297635425 10 25 4.8241310301148035 10 26 6.328070957102395 10 27 5.800599167003833 10 28 5.612963436721044 10 29 4.426597503803386 10 30 3.3494535721209173 10 31 6.280986435246071 10 32 8.269306459823703 10 33 8.051293246982903 10 34 8.252324526386392 10 35 8.159651209169464 10 36 6.793185648586256 10 37 2.1048656549670755 10 38 6.9298219331941455 10 39 6.226615023471006 10 40 6.838788254229431 11 1 -0.8826145344400942 11 2 4.915089539151408 11 3 -1.5206698558371983 11 4 0.46374963215734333 11 5 1.8949537728442376 11 6 -3.527847603151863 11 7 -2.749533070603052 11 8 -1.4743457522869292 11 9 -1.345902837865312 11 10 -0.9955339454325234 11 11 0.318722324553967 11 12 0.9095740888590758 11 13 -0.6751914920779036 11 14 -3.937497517695286 11 15 2.164165322507106 11 16 2.816210575541204 11 17 2.0245911458386114 11 18 2.4117612132064474 11 19 3.499276906325587 11 20 5.418134719123563 11 21 -3.166278169092573 11 22 -2.8890612852326836 11 23 -3.320570239992147 11 24 -2.894183369031241 11 25 -2.8425356365518626 11 26 -3.338595709564271 11 27 -3.199400832996167 11 28 -3.387036563278956 11 29 -2.906735829529948 11 30 -2.6505464278790827 11 31 -3.3856802314205954 11 32 -3.064026873509631 11 33 -3.282040086350431 11 34 -3.081008806946942 11 35 -3.173682124163869 11 36 -3.206814351413744 11 37 -2.8951343450329245 11 38 -3.0701780668058545 11 39 -3.1067183098623277 11 40 -3.1612117457705686 12 1 10.984052132226573 12 2 16.048422872484743 12 3 14.945996810829469 12 4 14.73041629882401 12 5 10.42828710617757 12 6 1.1388190635148039 12 7 15.98380026273028 12 8 15.658987581046405 12 9 16.320763828801354 12 10 11.871132721234142 12 11 8.318722324553965 12 12 4.709574088859075 12 13 6.191475174588762 12 14 5.262502482304713 12 15 3.5641653225071064 12 16 6.549543908874538 12 17 5.224591145838611 12 18 7.611761213206447 12 19 5.0992769063255885 12 20 8.284801385790232 12 21 6.767055164240761 12 22 4.310938714767316 12 23 5.6127630933411865 12 24 4.639149964302092 12 25 7.157464363448137 12 26 5.394737623769061 12 27 8.400599167003833 12 28 4.67963010338771 12 29 3.093264170470052 12 30 4.949453572120917 12 31 10.14765310191274 12 32 9.269306459823701 12 33 7.051293246982903 12 34 8.785657859719725 12 35 7.692984542502798 12 36 10.593185648586257 12 37 9.038198988300408 12 38 8.129821933194144 12 39 9.293281690137672 12 40 9.172121587562764 13 1 16.117385465559906 13 2 19.24842287248474 13 3 15.145996810829468 13 4 -2.8695837011759906 13 5 8.228287106177572 13 6 -1.8611809364851961 13 7 4.583800262730282 13 8 8.858987581046405 13 9 19.65409716213469 13 10 17.671132721234144 13 11 24.98538899122063 13 12 25.576240755525742 13 13 9.991475174588762 13 14 22.062502482304716 13 15 25.497498655840438 13 16 27.149543908874538 13 17 18.691257812505278 13 18 19.078427879873114 13 19 26.83261023965892 13 20 26.751468052456897 13 21 18.83372183090743 13 22 25.110938714767315 13 23 24.679429760007853 13 24 25.10581663096876 13 25 25.15746436344814 13 26 18.66140429043573 13 27 1.800599167003833 13 28 11.612963436721044 13 29 24.09326417047005 13 30 -2.6505464278790827 13 31 -3.3856802314205954 13 32 -3.064026873509631 13 33 -3.282040086350431 13 34 -3.081008806946942 13 35 -3.173682124163869 13 36 -3.206814351413744 13 37 -2.8951343450329245 13 38 -3.0701780668058545 13 39 -3.1067183098623277 13 40 -3.1612117457705686 15 1 6.844658192832632 15 2 3.793877417939287 15 3 5.327814992647651 15 4 3.4031435715512828 15 5 5.2282871061775715 15 6 -0.6793627546670145 15 7 13.401982080912099 15 8 11.222623944682768 15 9 14.290460798498325 15 10 11.21658726668869 15 11 7.1672071730388165 15 12 5.939877119162105 15 13 5.173293356406944 15 14 1.7897752095774404 15 15 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>>END_MODULE >>Per sequence quality scores fail #Quality Count 4 2327.0 5 82.0 6 66.0 7 75.0 8 81.0 9 155.0 10 191.0 11 167.0 12 127.0 13 108.0 14 67.0 15 64.0 16 51.0 17 38.0 18 41.0 19 67.0 20 120.0 21 166.0 22 185.0 23 200.0 24 157.0 25 107.0 26 94.0 27 62.0 28 34.0 29 15.0 30 15.0 31 4.0 32 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.30084310096649 0.41126876413736374 0.1850709438618137 0.10281719103434094 8 98.2322713257965 0.945529290853032 0.4933196300102775 0.328879753340185 9 95.88053553038105 1.9773429454170957 1.5242018537590112 0.6179196704428425 10 51.59235668789809 27.799465790014384 14.259297308403534 6.348880213683994 11 33.38812410108897 30.100678035750978 21.491678652147115 15.019519211012945 12 30.68228524455405 21.085080147965474 24.434854089601316 23.79778051787916 13 22.816032887975336 21.603288797533402 27.194244604316548 28.386433710174718 14 18.890030832476874 27.769784172661872 34.82014388489208 18.520041109969167 15 31.107458393260735 26.320115060612288 27.141976576946785 15.430449969180193 16 35.07294020957469 26.546126977604274 23.094308608999384 15.286624203821656 17 25.416067392644337 26.68995274296281 33.696322169714406 14.197657694678448 18 24.470926648859667 27.71727963838093 32.052599137045405 15.759194575713991 19 28.721217105263158 30.037006578947366 23.293585526315788 17.948190789473685 20 26.01191699198685 31.682761454694884 26.587220053421 15.718101499897266 21 22.375179782206697 26.68995274296281 32.689541812204645 18.24532566262585 22 20.916375590712967 24.121635504417505 30.778713786726936 24.183275118142593 23 20.941224825318535 26.531031648170984 29.86025482942869 22.66748869708179 24 22.80665707828231 27.141976576946785 31.867680295870144 18.18368604890076 25 27.842895332099527 29.138391939132223 23.072177668106107 19.946535060662143 26 22.464962901896126 27.59686727122836 24.546578730420446 25.39159109645507 27 18.37659662134322 27.441285537700864 26.34940255459415 27.832715286361765 28 20.254986633765164 35.30742340119268 21.900061690314622 22.537528274727535 29 29.888613861386137 29.434818481848186 21.823432343234323 18.853135313531354 30 22.219938335046248 27.60534429599178 32.31243576567317 17.8622816032888 31 24.51705713111385 31.935881627620223 24.126592683929303 19.42046855733662 32 31.922362172207308 25.934338220111503 23.311996696262646 18.831302911418543 33 25.16950893774399 27.737826176289293 24.779124717485104 22.31354016848161 34 20.834189439079516 31.785494144236697 30.408876104376414 16.971440312307376 35 18.03986028354222 31.33347031025272 33.799054859256216 16.827614546948837 36 19.348856377498453 36.65773748196992 25.716051926643313 18.277354213888316 37 23.97780973905897 29.607561125950276 30.347236490651326 16.067392644339428 38 27.53236079720567 29.422642284775012 25.005136634477093 18.03986028354222 39 22.31354016848161 30.71707417300185 22.416272858023422 24.553112800493118 40 21.614957879597288 27.943291555372916 31.189644544894186 19.252106020135606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.0 11 11.5 12 21.0 13 21.0 14 24.5 15 28.0 16 38.5 17 49.0 18 49.0 19 40.0 20 31.0 21 35.5 22 40.0 23 40.0 24 48.0 25 56.0 26 63.0 27 70.0 28 70.0 29 91.5 30 113.0 31 131.5 32 150.0 33 150.0 34 167.5 35 185.0 36 208.0 37 231.0 38 231.0 39 273.0 40 315.0 41 347.0 42 379.0 43 379.0 44 379.5 45 380.0 46 398.5 47 417.0 48 417.0 49 508.0 50 599.0 51 525.5 52 452.0 53 452.0 54 417.5 55 383.0 56 375.5 57 368.0 58 368.0 59 346.0 60 324.0 61 240.0 62 156.0 63 156.0 64 111.0 65 66.0 66 47.5 67 29.0 68 29.0 69 20.5 70 12.0 71 8.0 72 4.0 73 4.0 74 5.5 75 7.0 76 3.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.08218615163344976 8 0.04109307581672488 9 0.2465584549003493 10 0.0 11 0.0 12 0.02054653790836244 13 0.04109307581672488 14 0.04109307581672488 15 0.0 16 0.0 17 0.0 18 0.0 19 0.061639613725087326 20 0.0 21 0.0 22 0.0 23 0.02054653790836244 24 0.0 25 0.08218615163344976 26 0.3081980686254366 27 0.2671049928087117 28 0.08218615163344976 29 0.3903842202588864 30 0.04109307581672488 31 0.02054653790836244 32 0.4931169098006986 33 0.0 34 0.0 35 0.0 36 0.28765153071707417 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 4867.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.58311074583933 #Duplication Level Percentage of deduplicated Percentage of total 1 94.99288087327955 82.24779124717485 2 3.4646416706217367 5.999589069241833 3 0.5695301376364499 1.4793507294020958 4 0.40341718082581873 1.3971645777686459 5 0.11865211200759374 0.513663447709061 6 0.11865211200759374 0.6163961372508732 7 0.07119126720455624 0.43147729607561125 8 0.04746084480303749 0.32874460653379906 9 0.023730422401518746 0.18491884117526197 >10 0.14238253440911247 1.910828025477707 >50 0.023730422401518746 1.0889665091432095 >100 0.023730422401518746 3.801109513047052 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 185 3.801109513047052 No Hit ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 53 1.0889665091432095 No Hit ATACAGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 25 0.513663447709061 No Hit ATACAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 18 0.36983768235052394 No Hit ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 14 0.28765153071707417 No Hit ATACAGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 13 0.2671049928087117 No Hit ATACAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.2465584549003493 No Hit ATACAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.22601191699198686 No Hit ATACAGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA 9 0.18491884117526197 No Hit ATACAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.16437230326689953 No Hit ATACAGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG 8 0.16437230326689953 No Hit ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 7 0.14382576535853708 No Hit ATACAGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT 7 0.14382576535853708 No Hit ATACAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 7 0.14382576535853708 No Hit ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 6 0.12327922745017465 No Hit ATACAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC 6 0.12327922745017465 No Hit ATACAGGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAG 6 0.12327922745017465 No Hit ATACAGGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 6 0.12327922745017465 No Hit ATACAGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 6 0.12327922745017465 No Hit ATACAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG 5 0.10273268954181221 No Hit ATACAGGGGGGGCTAGAGAGATGGCTCAGAGGTTAAGAGC 5 0.10273268954181221 No Hit ATACAGGGGAAGGAAAAAAATCTATCTGAAAGGATTTTTG 5 0.10273268954181221 No Hit ATACAGGGGGATGGTAGTCGCCGTGCCTACCATGGTGACC 5 0.10273268954181221 No Hit ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG 5 0.10273268954181221 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGTA 25 1.6824547E-4 34.208336 5 AGGGGAT 50 2.382876E-10 34.208336 5 AGGGGAG 35 7.836479E-7 34.208336 5 AGGGGAC 25 1.6824547E-4 34.208336 5 TAGCATA 25 1.6824547E-4 34.208336 30 CAGGGGA 140 0.0 34.208336 4 AGCATAT 25 1.6824547E-4 34.208336 31 AGGGGGT 40 5.3032636E-8 34.208332 5 AGGGGGG 130 0.0 34.208332 5 AGGGGGA 65 0.0 34.208332 5 AGGGGAA 30 1.1511505E-5 34.208332 5 GTACCTG 20 0.0024481295 34.208332 9 GGGTACC 20 0.0024481295 34.208332 7 CAGGGGT 45 3.5652192E-9 34.208332 4 CAGGGGG 250 0.0 34.208332 4 CAGGGGC 20 0.0024481295 34.208332 4 GGGGTAC 20 0.0024481295 34.208332 6 ACAGGGG 455 0.0 34.208332 3 GGTACCT 20 0.0024481295 34.208332 8 ATCCTGC 20 0.002578567 33.85567 20 >>END_MODULE