FastQCFastQC Report
Fri 17 Jun 2016
SRR1526098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140956
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG33452.3730809614347743No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG8560.6072817049291978No Hit
TAAGGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT4430.31428247112574137No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG4100.2908709100712279No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG3760.2667499077726383No Hit
TAAGGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3390.24050058174182012No Hit
TAAGGGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2950.20928516700246888No Hit
TAAGGGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2860.20290019580578333No Hit
TAAGGGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2840.20148131331763106No Hit
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG2430.17239422231050824No Hit
TAAGGGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT2430.17239422231050824No Hit
TAAGGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1980.1404693663270808No Hit
TAAGGGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1980.1404693663270808No Hit
TAAGGGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC1680.11918612900479583No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1670.11847668776071967No Hit
TAAGGGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG1640.11634836402849115No Hit
TAAGGGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT1620.11492948154033884No Hit
TAAGGGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG1560.11067283407588183No Hit
TAAGGGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1560.11067283407588183No Hit
TAAGGGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1520.10783506909957717No Hit
TAAGGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1510.107125627855501No Hit
TAAGGGGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG1480.10499730412327252No Hit
TAAGGGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG1450.10286898039104402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAA150.00805415150.5582438
CGTAATT150.00805415150.5582441
GACCTAA150.00805415150.5582439
TAGACCT150.00810019650.48541637
TTGACTA150.00810019650.48541637
ACGACAA150.00810019650.48541637
ACTATTA204.0841295E-450.21418447
GTATCCA150.00827460750.21418442
ATTATAC150.00827460750.21418442
ATTCGCC150.00835694850.08860444
AATTCGC150.00836876150.07071743
AGAGGGT150.0084280149.9814612
TGATAAC150.00847563449.9102930
AACGGTT150.00847563449.9102929
GACGCAC355.3196345E-849.9102869
CCGGGTA150.00848757149.8925316
GTGGCAC150.00848757149.8925316
CAGCGAG150.00857148249.76854722
GGACGCA355.4707925E-849.733248
TAAGGGG141250.049.169891
Produced by FastQC (version 0.11.5)