##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526098_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 140956 Sequences flagged as poor quality 0 Sequence length 56 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.26294020829195 39.0 38.0 39.0 33.0 40.0 2 34.96945146003008 39.0 35.0 39.0 20.0 40.0 3 35.480866369647266 39.0 36.0 39.0 25.0 40.0 4 36.39503107352649 39.0 36.0 39.0 31.0 40.0 5 36.04436845540452 38.0 35.0 39.0 30.0 40.0 6 36.30659212803996 39.0 36.0 39.0 31.0 40.0 7 36.6721672011124 38.0 37.0 39.0 33.0 40.0 8 36.5196160503987 38.0 37.0 39.0 32.0 40.0 9 35.439427906580775 38.0 35.0 39.0 29.0 40.0 10 34.480582593149634 38.0 35.0 39.0 25.0 40.0 11 34.13210505405942 38.0 32.0 39.0 25.0 40.0 12 33.59842078379069 37.0 32.0 39.0 22.0 40.0 13 33.23860637362014 37.0 32.0 39.0 19.0 40.0 14 33.76775731433923 38.0 33.0 39.0 22.0 40.0 15 32.75634949913448 37.0 31.0 39.0 17.0 40.0 16 32.17567184085814 36.0 29.0 38.0 18.0 40.0 17 31.274170663185675 35.0 27.0 38.0 15.0 40.0 18 30.56436050966259 35.0 26.0 38.0 13.0 40.0 19 31.7397485740231 35.0 28.0 38.0 17.0 40.0 20 32.356387808961664 36.0 29.0 38.0 20.0 40.0 21 32.63053718891001 36.0 30.0 39.0 19.0 40.0 22 31.94309571781265 36.0 29.0 39.0 16.0 40.0 23 32.61321972814211 36.0 30.0 39.0 19.0 40.0 24 31.964364766310055 36.0 29.0 39.0 17.0 40.0 25 32.36881012514544 36.0 30.0 39.0 19.0 40.0 26 32.898308692074124 37.0 31.0 39.0 18.0 40.0 27 33.14468344731689 37.0 32.0 39.0 20.0 40.0 28 33.02744828173331 37.0 31.0 39.0 20.0 40.0 29 32.576974374982264 36.0 31.0 39.0 18.0 40.0 30 32.25157495956185 36.0 30.0 39.0 17.0 40.0 31 33.0449218195749 37.0 32.0 39.0 19.0 40.0 32 32.90330315843242 37.0 32.0 39.0 18.0 40.0 33 32.77727801583473 37.0 32.0 39.0 18.0 40.0 34 32.57494537302421 36.0 31.0 39.0 17.0 40.0 35 32.47799313260876 36.0 31.0 39.0 17.0 40.0 36 31.772389965663045 36.0 30.0 38.0 11.0 40.0 37 31.478496835892052 36.0 30.0 38.0 10.0 40.0 38 30.850719373421494 35.0 29.0 38.0 4.0 39.0 39 29.831819858679303 35.0 27.0 38.0 4.0 39.0 40 27.734754107664802 33.0 19.0 38.0 4.0 39.0 41 25.903090326059196 31.0 15.0 37.0 4.0 39.0 42 26.79938420500014 32.0 19.0 37.0 4.0 39.0 43 26.73212208064928 32.0 19.0 37.0 4.0 39.0 44 25.73649933312523 31.0 16.0 36.0 4.0 39.0 45 26.701346519481255 32.0 20.0 37.0 4.0 39.0 46 24.728681290615512 29.0 13.0 36.0 4.0 39.0 47 23.778540821249184 28.0 9.0 35.0 4.0 39.0 48 24.391696699679333 29.0 11.0 35.0 4.0 39.0 49 23.364354834132637 28.0 6.0 35.0 4.0 38.0 50 23.947714180311586 29.0 4.0 35.0 4.0 39.0 51 24.200949232384573 30.0 4.0 36.0 4.0 39.0 52 23.97456653139987 30.0 4.0 36.0 4.0 39.0 53 23.71833054286444 29.0 4.0 35.0 4.0 39.0 54 23.379969635914755 29.0 4.0 35.0 4.0 38.0 55 23.034223445614234 29.0 4.0 35.0 4.0 38.0 56 22.243068759045375 28.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.020276581720573 1 2 4.854130432272093 1 3 4.286713614522306 1 4 2.895736863842153 1 5 3.664184202506874 1 6 3.23191115851224 1 7 2.7365486763485336 1 8 2.8669211446696394 1 9 3.547872344324986 1 10 4.329576758018192 1 11 -2.074845854538193 1 12 -8.604461179802371 1 13 -18.58734370270728 1 14 -8.079185137178673 1 15 0.07788586447175305 1 16 -2.318677380227374 1 17 -7.219401133442826 1 18 -5.486284975994966 1 19 -1.2618945720564803 1 20 -2.3347115124606184 1 21 4.964448244093756 1 22 6.102387044313424 1 23 4.972197419067886 1 24 5.661305307823039 1 25 4.729224193681844 1 26 4.922037876860987 1 27 4.381370560128676 1 28 4.839869840030758 1 29 5.126858560826577 1 30 5.168960960928775 1 31 3.641426715973818 1 32 4.108486205172444 1 33 4.089027530882571 1 34 3.8802736379502 1 35 4.446587670652264 1 36 2.5746810884303244 1 37 2.489236399963417 1 38 3.546597972156757 1 39 3.0605112268915846 1 40 -10.91163907892272 1 41 -1.4205427529630832 1 42 -2.41681660549693 1 43 -9.781636325047455 1 44 -15.233564000761868 1 45 -4.586317884003208 1 46 -6.099148800214085 1 47 -9.974025995227166 1 48 -6.0511973933379775 1 49 -13.413492454340293 1 50 -4.607036275430975 1 51 0.7186489123501261 1 52 0.6711761329419872 1 53 0.5995841256488887 1 54 0.8705545356211601 1 55 0.8014277333030542 1 56 3.651715817747551 2 1 2.3646133594938803 2 2 5.038059506294047 2 3 4.422212790269164 2 4 3.199886274802708 2 5 3.579587684473829 2 6 3.112756319543159 2 7 2.932057501888316 2 8 3.093470137472501 2 9 4.011142819979362 2 10 4.522612824119278 2 11 2.742310673144651 2 12 -2.0522316755604812 2 13 0.2818412918647155 2 14 3.760467470275792 2 15 4.365369278890153 2 16 3.9266283975861462 2 17 6.442333416836615 2 18 4.00247703598686 2 19 5.615231397948751 2 20 4.752920669598808 2 21 6.696385037950055 2 22 7.016081708782082 2 23 6.099212818529107 2 24 6.799397865420243 2 25 5.817439384064215 2 26 5.2108682415427445 2 27 5.312334333197715 2 28 4.557754122930518 2 29 5.248425848028539 2 30 5.334836880674661 2 31 4.133948126451887 2 32 4.639787720994082 2 33 3.3378715661043152 2 34 3.7663256664170923 2 35 4.411828312363497 2 36 4.501423077091815 2 37 4.97393745741989 2 38 4.719128228278347 2 39 4.503858900889416 2 40 3.3359786284473074 2 41 6.90182064215573 2 42 5.766408837790433 2 43 4.887878370783032 2 44 -5.652425325195949 2 45 5.569104093400192 2 46 3.9635547501055655 2 47 0.7049575599173785 2 48 3.614175933026061 2 49 -2.6693526931726694 2 50 4.631172883830818 2 51 6.816513895664027 2 52 7.196064557211216 2 53 7.034689268184373 2 54 7.274599407158291 2 55 6.8040613226239515 2 56 6.932484584584429 3 1 2.557742593566367 3 2 5.37273170116319 3 3 4.849599604025499 3 4 3.2783855159088446 3 5 3.6369304453667297 3 6 3.498589066738951 3 7 2.868032773054054 3 8 2.9933886952039614 3 9 4.030577906789418 3 10 4.726958879722133 3 11 4.012606456656293 3 12 3.7477720263608134 3 13 4.491129412399275 3 14 4.460093576224779 3 15 -0.4808899296390763 3 16 5.800096727649443 3 17 6.845139473179859 3 18 7.355862443013105 3 19 6.183587374700682 3 20 5.0618274922397575 3 21 6.5978398930198345 3 22 7.350642846012306 3 23 6.391479504939351 3 24 6.915989876674086 3 25 6.478565508522806 3 26 5.844618658008894 3 27 4.681119732820118 3 28 5.549184175127607 3 29 5.798253708688222 3 30 5.8956224283739225 3 31 4.427232841218849 3 32 4.000799085892471 3 33 4.195976922784428 3 34 4.644643513379666 3 35 4.683271691477259 3 36 5.531260562746873 3 37 5.848109152529656 3 38 6.382253390237398 3 39 7.365129400278594 3 40 8.905130518278128 3 41 11.054469366843843 3 42 9.905823193841222 3 43 10.120414557063711 3 44 10.830175644707435 3 45 9.617210560656698 3 46 11.432162457505708 3 47 12.182098195907457 3 48 11.479481526629144 3 49 11.845492011228913 3 50 10.808957654126598 3 51 10.656405799562151 3 52 10.418346421498782 3 53 10.535022441101116 3 54 10.265196527063523 3 55 10.875600951691439 3 56 10.024921559374345 4 1 2.2749534611998 4 2 5.413343525481125 4 3 4.716041909899843 4 4 3.046588045639801 4 5 3.542944701100218 4 6 3.6398418318596697 4 7 3.0074893913823075 4 8 3.0912881487972044 4 9 4.250728783119982 4 10 4.362227640002708 4 11 4.8842481348246025 4 12 4.755807419906027 4 13 5.325751559834565 4 14 4.777274192964867 4 15 5.164626320762697 4 16 6.776954794239227 4 17 7.425238166719399 4 18 8.126976621228682 4 19 6.861850217150245 4 20 6.592409254732431 4 21 6.44244483525954 4 22 6.918433207321414 4 23 5.875928655078315 4 24 6.7847009995494325 4 25 6.2019021820589515 4 26 4.918752253516633 4 27 4.694921702909543 4 28 4.372140821816082 4 29 5.087698037591053 4 30 5.021333797037773 4 31 4.34029933646379 4 32 4.608085769827351 4 33 4.109213579304441 4 34 4.405377853815654 4 35 4.071636441623781 4 36 4.661339404137657 4 37 4.725082550650317 4 38 5.127106011666381 4 39 5.702275606627506 4 40 0.26379267251987315 4 41 9.617098169475671 4 42 8.291276295503131 4 43 8.627608597637717 4 44 9.166830274039452 4 45 8.243158264424824 4 46 10.227647511673712 4 47 10.808178247333977 4 48 10.493661632885406 4 49 10.668278496642312 4 50 10.807445109459263 4 51 9.876707930359448 4 52 9.761920942683862 4 53 10.017864512531037 4 54 9.975468596035562 4 55 10.150997521996405 4 56 9.733076065812114 5 1 2.3959177731512398 5 2 5.463337725689918 5 3 4.712658698362127 5 4 3.2588990691096242 5 5 3.7394029619361646 5 6 3.619039914062043 5 7 3.076390910927607 5 8 3.39875441334501 5 9 4.304995496184979 5 10 4.905571412960214 5 11 5.037033967867941 5 12 5.276029358582903 5 13 5.717604786451737 5 14 5.669166084856762 5 15 5.9425136501521685 5 16 7.225742637876852 5 17 8.390323423650155 5 18 8.67467023759183 5 19 7.856127756492167 5 20 7.152572422191739 5 21 7.054322807652529 5 22 8.061997372344806 5 23 6.762813393894387 5 24 7.560848391576652 5 25 7.113184709221308 5 26 6.245995806082075 5 27 5.374753895617278 5 28 5.30463125216059 5 29 5.9392427153159915 5 30 6.241377875450954 5 31 4.82608984800537 5 32 5.002684230949416 5 33 4.984324741969257 5 34 5.154962202112447 5 35 5.075580299644898 5 36 6.379952867386315 5 37 6.20120674351271 5 38 7.050606765639234 5 39 8.115298071152115 5 40 9.355700030521653 5 41 11.452770751929368 5 42 10.815693416486774 5 43 11.122756105660763 5 44 11.232425912596465 5 45 10.208630174102748 5 46 12.458633862831736 5 47 12.666044697475108 5 48 11.593205382643745 5 49 13.148385212800534 5 50 12.083553836211767 5 51 11.477237644623067 5 52 10.629859696888708 5 53 10.668272431179297 5 54 11.536617728000142 5 55 11.191789386822602 5 56 10.574471108923895 6 1 2.6494312866647505 6 2 5.603157545509738 6 3 4.698664704368134 6 4 3.561361531572082 6 5 3.9715951541283516 6 6 3.439460334482469 6 7 3.1058203403570346 6 8 3.115150809741408 6 9 4.2242147154041945 6 10 4.894340181728985 6 11 5.065802736636712 6 12 5.097590920144469 6 13 5.755622804469759 6 14 4.96838530407598 6 15 6.595546683185201 6 16 7.150307202441422 6 17 8.074527627854355 6 18 9.294129697051286 6 19 7.438349978714388 6 20 6.770530380149694 6 21 6.974442927772653 6 22 7.624279654627088 6 23 6.682032613113602 6 24 7.608716259444517 6 25 7.1503618863984855 6 26 5.774584394670665 6 27 5.79042957129295 6 28 5.620667288196628 6 29 6.57834181441509 6 30 6.161858355931432 6 31 4.917981739897268 6 32 5.019320867586057 6 33 4.588889306533819 6 34 4.728715955866203 6 35 5.011436155500753 6 36 6.03598890342235 6 37 6.4816872239931875 6 38 6.81403018906266 6 39 7.422084857938902 6 40 5.099844174665797 6 41 11.35211009126871 6 42 10.205363086156446 6 43 10.83422757713224 6 44 11.708041528212082 6 45 10.309290834763406 6 46 12.63016539436327 6 47 13.41042908185949 6 48 12.403475652914018 6 49 12.677033861449182 6 50 12.29244272510066 6 51 11.913694101079528 6 52 11.307156994186009 6 53 11.543167326074197 6 54 11.934095205477622 6 55 11.18794554297876 6 56 11.278254892707679 7 1 2.7286801129558285 7 2 5.4333922872937706 7 3 5.062170181676429 7 4 3.553912392291963 7 5 4.01766714160879 7 6 3.939303839959777 7 7 2.9878234702474913 7 8 3.471207147769576 7 9 4.467094214621724 7 10 5.147736112871392 7 11 5.157132940079592 7 12 5.630235677577957 7 13 5.96801717066694 7 14 5.209199075593972 7 15 6.685249343592087 7 16 7.497662445007929 7 17 8.306577706101617 7 18 9.185772889539553 7 19 7.719977521750383 7 20 6.837447438052671 7 21 6.837979703985486 7 22 8.054764787647429 7 23 7.0375881686691635 7 24 7.526525336126834 7 25 6.788796941171562 7 26 5.993426335202745 7 27 5.70535914875774 7 28 5.833625034675499 7 29 5.2378723308470185 7 30 6.211686211956472 7 31 4.628779861962993 7 32 4.8842347955985765 7 33 4.8994370373632385 7 34 4.571470259465581 7 35 4.316412681322348 7 36 5.878430217976337 7 37 6.0963977091262045 7 38 6.532715635072048 7 39 7.523368113024976 7 40 7.87073619344514 7 41 11.485380826792966 7 42 10.702890472697916 7 43 10.530064822828635 7 44 11.110169853720688 7 45 9.715926202525534 7 46 11.94791187323651 7 47 12.180319535693606 7 48 11.419813681083028 7 49 12.125672359101763 7 50 11.430971676587358 7 51 11.422739484491103 7 52 10.844997369772862 7 53 11.332337905103927 7 54 10.798132764163068 7 55 11.293172460036665 7 56 9.96858353120533 8 1 2.538002715236182 8 2 5.526014688366878 8 3 5.1440615297649614 8 4 3.5435837537943087 8 5 4.1204840430172425 8 6 3.7839047789269102 8 7 3.1169314514681474 8 8 3.2522936668842632 8 9 4.532151223340705 8 10 5.020371927760735 8 11 4.970882101716079 8 12 5.350289332842877 8 13 5.6641942330411865 8 14 5.1534646691553405 8 15 5.867610175248696 8 16 7.301418313552528 8 17 8.186591119917846 8 18 8.519843982765575 8 19 7.322794423158829 8 20 6.904181173800488 8 21 7.2538080071377316 8 22 7.9617399720874005 8 23 7.109016740097733 8 24 7.474748005476261 8 25 6.777663473700066 8 26 5.6425209026071705 8 27 5.298048618911995 8 28 5.550191097720436 8 29 6.105008481081761 8 30 6.325032959106036 8 31 4.9513150732305995 8 32 5.226622454887643 8 33 4.898095655740168 8 34 4.610303257453506 8 35 4.4457218697864676 8 36 4.892179379612827 8 37 5.332480874786835 8 38 5.378792093824561 8 39 6.662402318256358 8 40 1.980796555618177 8 41 10.227030726189344 8 42 8.964728165521528 8 43 8.854545037449697 8 44 9.52740660757716 8 45 8.7629416284142 8 46 11.342546346744218 8 47 11.718683050113455 8 48 11.434904224342588 8 49 11.77798624240156 8 50 11.202601455259387 8 51 10.568614736000157 8 52 10.16049032751934 8 53 10.171103834010705 8 54 10.337904729287143 8 55 10.255882050915268 8 56 9.634445368898156 9 1 2.478058737645142 9 2 5.150804604333267 9 3 4.638599344891013 9 4 3.2818190479119522 9 5 3.8057781606643033 9 6 3.278022425985732 9 7 2.9404608632328504 9 8 2.8098566920943497 9 9 3.967025172920536 9 10 4.185778090225718 9 11 4.547632801996194 9 12 5.059813142366686 9 13 5.500328686822698 9 14 5.2527643890433 9 15 6.351363676649253 9 16 6.930830078258413 9 17 7.681128935043898 9 18 8.702757148031676 9 19 7.434559129041187 9 20 6.793536916097409 9 21 7.053527895092909 9 22 7.345913641555192 9 23 6.763078364747596 9 24 6.984271815000074 9 25 6.0534337818233155 9 26 5.414649754147785 9 27 4.780821728155697 9 28 5.012235915647608 9 29 5.582459461473917 9 30 5.017609989918359 9 31 4.139550367348246 9 32 4.034745704187362 9 33 4.454118064703753 9 34 4.255121184624375 9 35 4.161828312363497 9 36 5.072851648520384 9 37 5.129399642293844 9 38 5.439716463572463 9 39 5.938032570357198 9 40 3.3184716256461897 9 41 7.952240810222957 9 42 8.114448053476707 9 43 8.12037136798191 9 44 9.222224534748026 9 45 7.699356194240529 9 46 10.112714413971108 9 47 10.641932349833347 9 48 9.887985456835587 9 49 10.542131900664867 9 50 10.010024424447064 9 51 10.02519736905338 9 52 9.760490327519339 9 53 9.643232685551318 9 54 9.925859911359971 9 55 10.15840305931863 9 56 9.196490186825326 10 1 2.3273056828000165 10 2 5.30731213488172 10 3 4.948133303401953 10 4 3.3542117731179815 10 5 3.936426072002753 10 6 3.556368547042851 10 7 3.2256271036420614 10 8 3.215440664813869 10 9 4.072523894148162 10 10 4.531828104434297 10 11 4.542517712482123 10 12 5.187832466038188 10 13 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4.178396074432669 114 26 3.60808866817494 114 27 3.4145321353955147 114 28 4.007333954863292 114 29 3.3998802759535494 114 30 4.16715750123058 114 31 3.402597124512649 114 32 3.363252491517681 114 33 3.2548722125167266 114 34 3.1095706567209014 114 35 3.549384873449469 114 36 4.541629929063376 114 37 3.9705761128820747 114 38 4.768901983934452 114 39 5.826871182725224 114 40 7.2306134054350295 114 41 7.531426330584946 114 42 6.938354539147898 114 43 6.747219030123688 114 44 -2.8605054803349255 114 45 5.888948954421526 114 46 -0.2508602466623735 114 47 -0.8589726275422187 114 48 0.8609115503499147 114 49 -7.926043061627743 114 50 0.8476564003143316 114 51 2.257625725011149 114 52 2.3886954557244664 114 53 2.726415189322058 114 54 2.7756336670160806 114 55 2.7445267395599586 114 56 4.878767713220498 115 1 1.7005110988713241 115 2 4.217429845979218 115 3 3.9706208859017806 115 4 2.542175303090083 115 5 3.064615498416302 115 6 2.7350784878470975 115 7 2.553551169778004 115 8 2.6331789787554953 115 9 2.7675636981898037 115 10 3.5092384602892395 115 11 0.9787291842110477 115 12 -1.3674169045816704 115 13 -2.44747578707954 115 14 -5.673430032387245 115 15 -6.551839858285845 115 16 2.0704323980595696 115 17 3.440380523003025 115 18 4.174035800337673 115 19 3.065047944285592 115 20 2.734161053076143 115 21 5.603237919947926 115 22 5.820023003609869 115 23 4.760592863504844 115 24 5.047652096690218 115 25 5.0211913073687455 115 26 4.026290184968001 115 27 3.700664313076988 115 28 3.3876156450041393 115 29 3.749609420048891 115 30 4.690559451393096 115 31 3.274319768066281 115 32 2.769211321420169 115 33 2.87361544111911 115 34 3.0879021843482057 115 35 2.6027976578481713 115 36 4.0450968846430015 115 37 4.443815549501796 115 38 4.89891281817064 115 39 4.908344638846568 115 40 7.640689245088332 115 41 7.851578009891558 115 42 6.0315289703505 115 43 4.668562475410798 115 44 -2.802975686185416 115 45 5.427193808159338 115 46 0.7483813568045825 115 47 -8.653013797639728 115 48 0.9315507702849075 115 49 -2.8297267019311008 115 50 1.9028026625028502 115 51 3.556542301392515 115 52 3.6877203744676983 115 53 4.062384853460735 115 54 4.007052951956492 115 55 3.736834431867649 115 56 5.785484939656037 116 1 2.3662940317627914 116 2 5.226294800411694 116 3 4.7062464037145375 116 4 3.343583753794306 116 5 3.916562474389792 116 6 3.4564537985347528 116 7 3.193402039703443 116 8 3.3775037509178816 116 9 4.222907525861714 116 10 4.8259741686570905 116 11 3.4986131941530516 116 12 2.5754994168764895 116 13 4.070916922116815 116 14 4.277274192964867 116 15 -4.080008872370353 116 16 3.8131830194348844 116 17 5.486030895828211 116 18 5.43609048136501 116 19 5.269853246688246 116 20 4.468046720018975 116 21 5.377057306857619 116 22 6.525325406261075 116 23 5.957196011806413 116 24 6.760742403235366 116 25 6.336767115156654 116 26 5.911708577677199 116 27 5.466115845802758 116 28 5.313216307804467 116 29 5.950106520297446 116 30 5.726433519330126 116 31 5.135628798720791 116 32 4.334745704187359 116 33 4.51098080980179 116 34 5.027670204232219 116 35 4.394181253539969 116 36 6.343439883814504 116 37 6.005870230529137 116 38 7.177951757690106 116 39 7.457640413494456 116 40 9.605726527606976 116 41 8.434593751399426 116 42 5.195820602496315 116 43 1.2517439170015159 116 44 0.6290872798460683 116 45 5.193473841299351 116 46 -0.025520880146537195 116 47 -6.175714708990183 116 48 -1.1463282686546101 116 49 -1.8441426091390554 116 50 0.5404165813098096 116 51 4.158530702386713 116 52 4.4271569941860065 116 53 4.724605234570927 116 54 4.484683440771736 116 55 4.307422667161767 116 56 6.313156853491993 117 1 1.6689941281948037 117 2 3.95802093348788 117 3 3.313382372856296 117 4 2.3862067046139828 117 5 3.1264949719789925 117 6 2.8636861996919407 117 7 2.18250522195995 117 8 2.3115442523643566 117 9 2.4431582491018276 117 10 3.1834476498528446 117 11 0.0034347949245088216 117 12 -6.9920994259393225 117 13 -7.690606703726964 117 14 -1.099774987363002 117 15 -4.94066461007527 117 16 -7.590384965135996 117 17 0.39962781000371805 117 18 -2.576252816609916 117 19 0.45722065266702927 117 20 -0.18364084025103367 117 21 3.7873755325085305 117 22 4.410764171931277 117 23 3.7206482779587766 117 24 4.394107880573845 117 25 4.130981194230905 117 26 4.854620824543158 117 27 4.531303888232845 117 28 4.482743790928865 117 29 4.882604109497059 117 30 5.0178510699569365 117 31 4.381916166126775 117 32 3.892508481429406 117 33 4.255628832945472 117 34 3.8422314385620133 117 35 4.428703915063593 117 36 5.2001419088868275 117 37 5.357461358783713 117 38 6.569658604363202 117 39 7.146165003658393 117 40 8.606401551714974 117 41 6.627264918226814 117 42 4.65020825919834 117 43 -6.4868288891701305 117 44 -1.6264575610411072 117 45 4.528671526930982 117 46 -1.9333318733962948 117 47 -1.9723395884501649 117 48 -0.01903800828817026 117 49 -1.6947694172393462 117 50 0.4332806121680548 117 51 4.6950784362343505 117 52 5.558304535169611 117 53 4.692493373433031 117 54 5.128849303838276 117 55 4.867981972851254 117 56 7.295413362653033 118 1 0.7519389319246912 118 2 3.8672554048369534 118 3 3.3297758154792447 118 4 1.8334920106750445 118 5 2.5054993335371236 118 6 2.0221310786211006 118 7 1.1490415432112684 118 8 1.7584535620349868 118 9 1.9253360201952248 118 10 2.4791312112906567 118 11 -1.7930759585722562 118 12 -9.070156276593558 118 13 -8.002909305621987 118 14 -9.800707458411278 118 15 -7.685513459526316 118 16 -10.171976181860316 118 17 -1.0802939827470723 118 18 -6.254249507841678 118 19 -1.7524349346393322 118 20 -0.9011923517565208 118 21 3.4455948136034387 118 22 5.208703711711692 118 23 4.3556309821248185 118 24 4.300785576467963 118 25 3.8739500617297793 118 26 4.599620072550376 118 27 3.892937216552891 118 28 3.686233037432096 118 29 4.024580346525184 118 30 4.934906266227049 118 31 3.1675230243009374 118 32 3.7060894710519072 118 33 2.9933337509782625 118 34 3.480071715295356 118 35 2.3289896723203256 118 36 3.9373956312510927 118 37 4.065988956918776 118 38 4.5000240728547105 118 39 5.989750505237577 118 40 7.334706559986898 118 41 5.193741080055659 118 42 5.294741271681421 118 43 -2.2569446960583868 118 44 -1.4939564330519346 118 45 4.629739356679817 118 46 -2.168856012364561 118 47 -1.8629246388336824 118 48 -1.4010503409697748 118 49 -2.744250542220545 118 50 0.5820247842240001 118 51 5.1380773834444575 118 52 5.106056076754815 118 53 3.71964031282241 118 54 4.2801502513491805 118 55 4.631329844711686 118 56 8.18088486212664 119 1 1.427803990076825 119 2 4.170296653121284 119 3 3.762846681620978 119 4 2.006575879778559 119 5 2.62888299314848 119 6 2.0324612093731034 119 7 1.924018065641377 119 8 1.5904264002925856 119 9 1.9236022919571312 119 10 2.711523033872467 119 11 -0.8499277970465755 119 12 -8.098979508551945 119 13 -3.6383929435617404 119 14 -6.395954153491825 119 15 -5.355599423551457 119 16 -6.704880899045897 119 17 -0.00827202218938794 119 18 -6.0338493055733835 119 19 -0.6425599075498312 119 20 -0.6790584212501294 119 21 3.5961402156116726 119 22 4.7712262862981305 119 23 3.6806327880917316 119 24 3.492145830748104 119 25 4.0117092179820375 119 26 4.128985094583175 119 27 3.584550306201084 119 28 3.259302458800299 119 29 4.097396932525328 119 30 4.117308924610352 119 31 3.3872730784799394 119 32 2.885602582371707 119 33 3.1665620974349586 119 34 3.611690584037685 119 35 3.044297047889202 119 36 4.627274992661192 119 37 4.68081233335452 119 38 5.267993443655836 119 39 5.5600026182188635 119 40 7.428978032933514 119 41 4.483273695818138 119 42 3.6445931823944164 119 43 -6.112684058913256 119 44 -2.6817047535027037 119 45 3.4117230307389086 119 46 -2.587262427159043 119 47 -4.103551670546459 119 48 -6.67064508199412 119 49 -3.8722574771334983 119 50 -0.8587033816360012 119 51 4.022495500904547 119 52 4.214558568989155 119 53 4.705337054858099 119 54 3.2345676464225015 119 55 4.307700573599934 119 56 5.867441244413715 120 1 1.3805598955886325 120 2 4.18604468461735 120 3 3.503004161935941 120 4 2.093190053006907 120 5 2.5501428356681615 120 6 2.5049021542549923 120 7 1.8452779081610586 120 8 2.2124736443870745 120 9 2.25431095337445 120 10 2.396562403951208 120 11 -2.08614826948752 120 12 -9.957247225087379 120 13 -8.465164597105048 120 14 -6.6951667519170215 120 15 -1.631189974732557 120 16 -9.657636804557708 120 17 -1.06339013242561 120 18 -6.978731195337165 120 19 -2.2646071516443165 120 20 -1.9546489724312295 120 21 1.7693685620683617 120 22 3.5586278611012787 120 23 2.964097355020865 120 24 2.7441143346851113 120 25 3.3660399266434524 120 26 4.270717378047745 120 27 3.820770778642032 120 28 3.79473552966644 120 29 4.191885121501706 120 30 4.266915223822945 120 31 3.0565644170626207 120 32 2.649382109930759 120 33 3.3240424123955883 120 34 3.4857063320691815 120 35 3.493115945526995 120 36 4.540660819432844 120 37 4.066639105008061 120 38 4.433347774364496 120 39 5.331656161525952 120 40 5.641576458130363 120 41 -7.571844414418081 120 42 -6.3711548491016465 120 43 -8.28591240536995 120 44 -15.437610265313728 120 45 -5.619773032253217 120 46 -9.666002584639356 120 47 -8.84370915086142 120 48 -6.906865554435065 120 49 -14.604540941700428 120 50 -5.685475035179307 120 51 -0.9775044990954527 120 52 -1.5177248955777713 120 53 -0.6804897167954458 120 54 -0.22999928271135772 120 55 0.08722813265504925 120 56 2.3792522680357635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2679.0 5 377.0 6 340.0 7 554.0 8 544.0 9 661.0 10 534.0 11 444.0 12 441.0 13 385.0 14 464.0 15 585.0 16 683.0 17 844.0 18 1077.0 19 1381.0 20 1850.0 21 2458.0 22 3070.0 23 3430.0 24 4128.0 25 4346.0 26 4709.0 27 4941.0 28 5244.0 29 5564.0 30 6130.0 31 6911.0 32 7664.0 33 8323.0 34 9185.0 35 9779.0 36 10588.0 37 11394.0 38 10574.0 39 8673.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.07559805896877 0.7491699537444308 0.1177672465166435 0.05746474077016942 8 97.92985044978575 1.4387468429864638 0.43417804137461335 0.19722466585317405 9 92.03073343076464 3.3450629283312288 3.2975296905373384 1.3266739503667864 10 42.3295212690485 32.75419279775249 14.92025880416584 9.996027129033173 11 30.89120009080848 27.87891256846108 22.20267317460768 19.02721416612276 12 27.253894832429975 17.44019410312438 22.848974147961066 32.45693691648457 13 24.750986123329266 15.899997162235024 24.25650557620818 35.092511138227536 14 23.02775334146826 24.617611169442945 28.35636652572434 23.998268963364456 15 31.279445200744107 20.79570643535776 22.727953072992022 25.196895290906113 16 34.02506123318306 22.81069184622484 21.571829186042386 21.592417734549716 17 26.12638824823475 21.664123762551892 30.496398538125817 21.713089451087537 18 23.58821192428843 21.862850818695197 29.017565765203322 25.531371491813047 19 30.09346988310942 24.131837247429047 20.89694182440153 24.877751045060005 20 26.13544345157762 27.48788907672856 24.451989600943303 21.92467787075052 21 24.124549504810012 21.267629614915293 29.057294474871593 25.550526405403108 22 21.689747155140612 20.73129203439371 26.87434376684923 30.70461704361645 23 20.770311302817902 22.932688214762052 25.458299043673204 30.838701438746842 24 21.221903734816667 22.938188216596664 29.6195652173913 26.22034283119537 25 30.014615319124783 25.476771575332748 22.568606862105174 21.940006243437296 26 27.55147669441901 24.08988636444293 21.147144205722235 27.211492735415828 27 22.65624445343727 24.330330202409602 21.153259071514274 31.86016627263885 28 24.594362579903656 28.331831629431502 23.152726834148037 23.9210789565168 29 32.47761830325423 23.07162142958647 20.99971578797783 23.45104447918147 30 26.944700787960528 22.896997231490026 28.206857386242635 21.951444594306807 31 27.240603691115638 29.365017419624927 22.41774460204494 20.97663428721449 32 31.957424161788182 24.168884158240196 21.7548341316303 22.118857548341317 33 25.557426073120666 25.091454102471218 22.66854191971928 26.68257790468884 34 24.08070729037852 29.737893195741478 25.21285517319802 20.968544340681987 35 23.282035979196863 25.03552448347401 31.330462955068633 20.351976582260495 36 26.334807578995196 28.366568935774477 23.966549058304878 21.332074426925452 37 25.833244520231624 23.207218728906888 29.10440868236882 21.855128068492665 38 30.21946381726278 24.947213513340586 22.825801039378362 22.00752163001827 39 25.290377579654034 23.621638901715624 22.840194650658898 28.24778886797144 40 20.78812801716431 18.915072411943502 27.090291435723223 33.20650813516896 41 19.461687334241272 16.456168016629633 24.073543115188876 40.00860153394022 42 27.887768821985247 24.99804363755362 23.833474428564312 23.280713111896816 43 20.464811877103 21.598742290482527 30.355758218150005 27.580687614264477 44 22.733750409713412 22.89051032477804 23.771928574482338 30.603810691026208 45 24.051838739718626 23.62387766853633 29.485238186639368 22.839045405105676 46 21.412610304582977 25.276117278679195 25.399231426131514 27.912040990606318 47 18.594587658263944 25.29086346526748 25.429166191399567 30.68538268506901 48 22.906729131175467 28.189948892674614 24.766467915956845 24.136854060193073 49 20.83949809366642 27.582086154896718 23.32626756956695 28.252148181869913 50 26.73016098653115 24.452894559152778 25.565940509612993 23.25100394470308 51 21.96005322441777 28.163401808776335 27.705871051751497 22.170673915054397 52 22.002657514584357 25.379974845984947 30.17131731718929 22.446050322241405 53 22.227201274637416 24.432921494569275 29.714984813890133 23.62489241690317 54 23.01335571138009 28.485072610124167 25.536575305446114 22.964996373049626 55 21.879291085924883 28.35158267699729 26.232808975973477 23.536317261104355 56 26.764189251655207 22.62102021803907 27.921232852357825 22.6935576779479 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 16.5 7 32.0 8 63.5 9 95.0 10 133.0 11 171.0 12 180.5 13 192.0 14 194.0 15 192.5 16 191.0 17 195.5 18 200.0 19 212.5 20 225.0 21 230.5 22 243.0 23 250.0 24 293.5 25 337.0 26 412.0 27 487.0 28 577.5 29 728.0 30 788.0 31 975.0 32 1162.0 33 1318.5 34 1475.0 35 1772.0 36 2069.0 37 2359.5 38 2784.5 39 2919.0 40 3195.5 41 3472.0 42 3961.0 43 4450.0 44 5516.5 45 6583.0 46 9432.5 47 11577.0 48 10872.0 49 9736.0 50 8600.0 51 8429.5 52 8259.0 53 8404.0 54 8961.0 55 9373.0 56 8858.0 57 8343.0 58 8316.5 59 8290.0 60 8388.5 61 8487.0 62 7725.5 63 6371.0 64 5778.0 65 5307.0 66 4836.0 67 4081.5 68 3327.0 69 3011.0 70 2695.0 71 2336.5 72 1718.0 73 1458.0 74 1050.5 75 643.0 76 494.0 77 345.0 78 374.0 79 429.5 80 456.0 81 294.5 82 133.0 83 81.0 84 29.0 85 16.0 86 3.0 87 3.5 88 3.0 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0014188824881523313 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.46326513238173617 16 0.07165356565169272 17 0.029087091007122787 18 0.0 19 0.03901926842418911 20 0.12344277646925282 21 0.0 22 0.0 23 0.0 24 0.008513294928913987 25 0.005675529952609325 26 0.047532563353103095 27 0.07307244813984506 28 0.003547206220380828 29 0.15323930872045177 30 0.06101194699055024 31 0.01631714861375181 32 0.021992678566361137 33 0.12415221771332899 34 0.1773603110190414 35 0.14756377876784246 36 0.0659780356990834 37 0.14969210250007095 38 0.20928516700246888 39 0.13550327761854764 40 0.8023780470501433 41 1.0258520389341355 42 0.2759726439456284 43 0.27171599648117145 44 0.4355969238627657 45 0.20502851953801185 46 0.3093163824172082 47 0.4852578109480973 48 0.053917534549788586 49 0.2639121427963336 50 0.18516416470387922 51 0.29725588126791336 52 0.15820539742898493 53 0.2603649365759528 54 0.24262890547404864 55 0.28590482136269474 56 0.24050058174182012 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 140956.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.04290991759022 #Duplication Level Percentage of deduplicated Percentage of total 1 89.556 63.6231884057971 2 6.406000000000001 9.10201761864166 3 1.644 3.5038363171355504 4 0.75 2.131287297527707 5 0.406 1.4421710713270814 6 0.23900000000000002 1.0187553282182438 7 0.192 0.9548167092924127 8 0.121 0.6876953680022734 9 0.084 0.5370843989769821 >10 0.512 6.819408922989485 >50 0.053 2.5333901676612673 >100 0.034999999999999996 4.661835748792271 >500 0.001 0.6081273088945723 >1k 0.001 2.3763853367433927 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 3345 2.3730809614347743 No Hit TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 856 0.6072817049291978 No Hit TAAGGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 443 0.31428247112574137 No Hit TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 410 0.2908709100712279 No Hit TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 376 0.2667499077726383 No Hit TAAGGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 339 0.24050058174182012 No Hit TAAGGGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 295 0.20928516700246888 No Hit TAAGGGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 286 0.20290019580578333 No Hit TAAGGGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 284 0.20148131331763106 No Hit TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 243 0.17239422231050824 No Hit TAAGGGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 243 0.17239422231050824 No Hit TAAGGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 198 0.1404693663270808 No Hit TAAGGGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 198 0.1404693663270808 No Hit TAAGGGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 168 0.11918612900479583 No Hit TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 167 0.11847668776071967 No Hit TAAGGGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 164 0.11634836402849115 No Hit TAAGGGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 162 0.11492948154033884 No Hit TAAGGGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 156 0.11067283407588183 No Hit TAAGGGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 156 0.11067283407588183 No Hit TAAGGGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 152 0.10783506909957717 No Hit TAAGGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 151 0.107125627855501 No Hit TAAGGGGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 148 0.10499730412327252 No Hit TAAGGGGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG 145 0.10286898039104402 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 7.094412440761656E-4 0.0 0.0 0.0 0.0 9 7.094412440761656E-4 0.0 0.0 0.0 0.0 10 0.0028377649763046625 0.0 0.0 0.0 0.0 11 0.007803853684837821 0.0 0.0 0.0 0.0 12 0.009222736172990152 0.0 0.0 0.0 0.0 13 0.009222736172990152 0.0 0.0 0.0 0.0 14 0.01631714861375181 0.0 0.0 0.0 0.0 15 0.01631714861375181 0.0 0.0 0.0 0.0 16 0.018445472345980304 0.0 0.0 0.0 0.0 17 0.01915491359005647 0.0 0.0 0.0 0.0 18 0.021992678566361133 0.0 0.0 0.0 0.0 19 0.023411561054513464 0.0 0.0 0.0 0.0 20 0.02908709100712279 0.0 0.0 0.0 0.0 21 0.03689094469196061 0.0 0.0 0.0 0.0 22 0.03901926842418911 0.0 0.0 0.0 0.0 23 0.0432759158886461 0.0 0.0 0.0 0.0 24 0.04611368086495077 0.0 0.0 0.0 7.094412440761656E-4 25 0.05462697579386475 0.0 0.0 0.0 7.094412440761656E-4 26 0.05604585828201708 0.0 0.0 0.0 7.094412440761656E-4 27 0.060302505746474076 0.0 0.0 0.0 0.0014188824881523313 28 0.06455915321093107 0.0 0.0 0.0 0.0014188824881523313 29 0.06881580067538806 0.0 0.0 0.0 0.0014188824881523313 30 0.07165356565169273 0.0 0.0 0.0 0.0014188824881523313 31 0.07732909560430205 0.0 0.0 0.0 0.0014188824881523313 32 0.08158574306875904 0.0 0.0 0.0 0.0014188824881523313 33 0.08300462555691138 0.0 0.0 0.0 0.0014188824881523313 34 0.08442350804506371 0.0 0.0 0.0 0.0014188824881523313 35 0.08726127302136837 0.0 0.0 0.0 0.0014188824881523313 36 0.0908084792417492 0.0 0.0 0.0 0.0014188824881523313 37 0.09293680297397769 0.0 0.0 0.0 0.0014188824881523313 38 0.09506512670620619 0.0 0.0 0.0 0.0028377649763046625 39 0.09577456795028236 0.0 0.0 0.0 0.0028377649763046625 40 0.09648400919435852 0.0 0.0 0.0 0.0028377649763046625 41 0.09719345043843469 0.0 0.0 0.0 0.0028377649763046625 42 0.09861233292658703 0.0 0.0 0.0 0.0028377649763046625 43 0.10357842163512018 0.0 0.0 0.0 0.0028377649763046625 44 0.107125627855501 0.0 0.0 0.0 0.0028377649763046625 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACAAA 15 0.008054151 50.55824 38 CGTAATT 15 0.008054151 50.55824 41 GACCTAA 15 0.008054151 50.55824 39 TAGACCT 15 0.008100196 50.485416 37 TTGACTA 15 0.008100196 50.485416 37 ACGACAA 15 0.008100196 50.485416 37 ACTATTA 20 4.0841295E-4 50.214184 47 GTATCCA 15 0.008274607 50.214184 42 ATTATAC 15 0.008274607 50.214184 42 ATTCGCC 15 0.008356948 50.088604 44 AATTCGC 15 0.008368761 50.070717 43 AGAGGGT 15 0.00842801 49.98146 12 TGATAAC 15 0.008475634 49.91029 30 AACGGTT 15 0.008475634 49.91029 29 GACGCAC 35 5.3196345E-8 49.910286 9 CCGGGTA 15 0.008487571 49.89253 16 GTGGCAC 15 0.008487571 49.89253 16 CAGCGAG 15 0.008571482 49.768547 22 GGACGCA 35 5.4707925E-8 49.73324 8 TAAGGGG 14125 0.0 49.16989 1 >>END_MODULE