FastQCFastQC Report
Fri 17 Jun 2016
SRR1526097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC110.38474991255683805No Hit
TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTAC100.3497726477789437No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.3147953830010493No Hit
TAAGGGGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC90.3147953830010493No Hit
TAAGGGGGGATGAGATGGCCTCAAATGAATTGACGGGTAC80.27981811822315494No Hit
TAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA70.24484085344526058No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.24484085344526058No Hit
TAAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.24484085344526058No Hit
TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTAC70.24484085344526058No Hit
TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTAC60.2098635886673662No Hit
TAAGGGGGGATGAGATGGCCTTAAATGAATTGACGGGTAC50.17488632388947184No Hit
TAAGGGGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC50.17488632388947184No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC40.13990905911157747No Hit
TAAGGGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC40.13990905911157747No Hit
TAAGGGGGGGGTTCTCTTTGAAAGATGTGTCATATCTTGT30.1049317943336831No Hit
TAAGGGGGGGCTCTTGCTTCTTGCTCTCTTTTCCTGAAGA30.1049317943336831No Hit
TAAGGGGGGCTGAGATGGACTCAAATGAATTGACGGGTAC30.1049317943336831No Hit
TAAGGGGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC30.1049317943336831No Hit
TAAGGGGGGGCTGGTGAGCTGGCTCCGTGGGTCAGAGCAC30.1049317943336831No Hit
TAAGGGGGGGGGCTGGAGAGATGGCTCCGCCGTTAAAGGC30.1049317943336831No Hit
TAAGGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.1049317943336831No Hit
TAAGGGGGGGTCTGTAAAATGCCAGGGTTTGCCTAGATTA30.1049317943336831No Hit
TAAGGGGGGATGAGATGGCCTTCAATGAATTGACGGGTAC30.1049317943336831No Hit
TAAGGGGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC30.1049317943336831No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCCGTGGGTCAGAGC30.1049317943336831No Hit
TAAGGGGGGAGATGATGCATTGAGCTTGTTTGTCAAATAC30.1049317943336831No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG30.1049317943336831No Hit
TAAGGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1049317943336831No Hit
TAAGGGGGGATGAGATGGCCTCCAATGAATTGACGGGTAC30.1049317943336831No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGA251.5664106E-434.03
GGCTCCG200.002349837233.99999623
TGGCTCC200.002349837233.99999622
TAAGGGG2800.033.3928571
AAGGGGG2800.032.7857132
AGGGGGG2400.032.5833363
GGGGATG304.5729693E-428.3333366
GGGGGGA1050.027.523814
GGATGAG250.00701271227.1999998
GATGAGA250.00701271227.1999999
GAATTGA250.00701271227.19999927
GGGATGA250.00701271227.1999997
AATTGAC250.00701271227.19999928
ATGAGAT250.00701271227.19999910
GGGGGAG407.3849726E-525.4999985
GATGGCT350.001127417424.28571320
ATGGCTC350.001127417424.28571321
GGGGAGT350.001127417424.2857136
GGGGGAT552.2770786E-521.6363625
GGGCTGG503.3658475E-420.410
Produced by FastQC (version 0.11.5)