##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2859
Sequences flagged as poor quality	0
Sequence length	40
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.077649527806926	34.0	32.0	37.0	4.0	38.0
2	20.399790136411333	22.0	11.0	30.0	4.0	34.0
3	20.898915704791886	23.0	11.0	30.0	4.0	35.0
4	25.386498775795733	31.0	15.0	36.0	4.0	38.0
5	24.848198670863937	30.0	14.0	36.0	4.0	38.0
6	24.488282616299404	30.0	13.0	35.0	4.0	37.0
7	25.61245190626093	31.0	18.0	35.0	4.0	37.0
8	23.972367960825462	29.0	11.0	34.0	4.0	37.0
9	22.627841902763205	27.0	14.0	31.0	4.0	35.0
10	17.77789436866037	18.0	5.0	28.0	4.0	32.0
11	22.263728576425322	27.0	8.0	32.0	4.0	36.0
12	22.802378454004895	28.0	7.0	33.0	4.0	36.0
13	23.43756558237146	28.0	4.0	34.0	4.0	37.0
14	23.83455753760056	30.0	4.0	35.0	4.0	37.0
15	21.40713536201469	26.0	4.0	33.0	4.0	36.0
16	19.53725078698846	23.0	4.0	31.0	4.0	35.0
17	19.679258481986707	23.0	4.0	32.0	4.0	35.0
18	19.174186778593914	22.0	4.0	31.0	4.0	35.0
19	16.948933193424274	16.0	4.0	29.0	4.0	34.0
20	18.295557887373207	19.0	4.0	32.0	4.0	35.0
21	15.044421126267926	10.0	4.0	27.0	4.0	33.0
22	15.381951731374606	8.0	4.0	28.0	4.0	34.0
23	15.175236096537251	4.0	4.0	28.0	4.0	33.0
24	14.878978663868486	4.0	4.0	28.0	4.0	33.0
25	14.487233298356069	4.0	4.0	29.0	4.0	33.0
26	12.180482686253935	4.0	4.0	23.0	4.0	32.0
27	11.921301154249738	4.0	4.0	22.0	4.0	32.0
28	11.852046169989507	4.0	4.0	23.0	4.0	32.0
29	11.116824064358168	4.0	4.0	19.0	4.0	31.0
30	10.5033228401539	4.0	4.0	16.0	4.0	30.0
31	10.56663168940189	4.0	4.0	16.0	4.0	31.0
32	9.829310947883876	4.0	4.0	12.0	4.0	30.0
33	9.770199370409234	4.0	4.0	11.0	4.0	30.0
34	9.865337530605107	4.0	4.0	9.0	4.0	31.0
35	9.335431969220007	4.0	4.0	4.0	4.0	30.0
36	9.62224554039874	4.0	4.0	4.0	4.0	33.0
37	9.549492829660721	4.0	4.0	4.0	4.0	32.0
38	9.43336831059811	4.0	4.0	4.0	4.0	32.0
39	9.200769499825114	4.0	4.0	4.0	4.0	32.0
40	9.26792584819867	4.0	4.0	4.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-19.024198082010585
1	2	-14.802728174603171
1	3	-15.694957010582005
1	4	-16.975661375661375
1	5	-17.771221891534395
1	6	-16.26796461640213
1	7	-17.420965608465604
1	8	-16.22572751322752
1	9	-14.706274801587305
1	10	-11.041087962962965
1	11	-14.955729166666664
1	12	-14.528389550264553
1	13	-17.743402777777774
1	14	-15.169394841269845
1	15	-13.817799272486777
1	16	-13.046560846560844
1	17	-12.461491402116398
1	18	-11.963128306878305
1	19	-11.264591600529098
1	20	-12.430109126984116
1	21	-7.311408730158731
1	22	-7.3229414682539655
1	23	-8.479092261904764
1	24	-8.119609788359789
1	25	-8.027430555555558
1	26	-7.6132275132275105
1	27	-4.807713293650793
1	28	-5.769196428571429
1	29	-5.0940806878306875
1	30	-4.747131283068781
1	31	-1.7833746693121704
1	32	-2.6560763888888888
1	33	-3.0125082671957655
1	34	-2.2740740740740746
1	35	-1.6402695105820095
1	36	-1.5357556216931219
1	37	-1.2544642857142865
1	38	-1.1915013227513231
1	39	-2.376951058201059
1	40	-0.7627314814814792
2	1	3.261516203703703
2	2	13.054414682539687
2	3	12.59075727513228
2	4	7.4529100529100525
2	5	7.800206679894178
2	6	-19.553678902116413
2	7	-20.420965608465604
2	8	-18.940013227513234
2	9	-17.99198908730159
2	10	-13.32680224867725
2	11	-17.67001488095238
2	12	-18.242675264550268
2	13	-18.314831349206347
2	14	-18.88368055555556
2	15	-16.96065641534392
2	16	-15.189417989417986
2	17	-15.60434854497354
2	18	-14.534556878306876
2	19	-11.978877314814811
2	20	-13.858680555555544
2	21	-10.025694444444445
2	22	-10.322941468253966
2	23	-9.33623511904762
2	24	-9.548181216931217
2	25	-8.884573412698414
2	26	-8.184656084656082
2	27	-7.5219990079365076
2	28	-7.483482142857143
2	29	-6.379794973544973
2	30	-5.8899884259259245
2	31	-6.3548032407407415
2	32	-5.941790674603174
2	33	-5.72679398148148
2	34	-5.988359788359789
2	35	-5.354555224867724
2	36	-5.964327050264551
2	37	-5.683035714285715
2	38	-5.620072751322752
2	39	-5.519808201058202
2	40	-5.619874338624337
6	1	4.011516203703703
6	2	6.8044146825396865
6	3	12.09075727513228
6	4	1.4529100529100525
6	5	5.300206679894178
6	6	3.4463210978835868
6	7	5.079034391534396
6	8	-4.190013227513234
6	9	3.75801091269841
6	10	2.17319775132275
6	11	-4.67001488095238
6	12	-2.992675264550268
6	13	-2.3148313492063473
6	14	0.3663194444444393
6	15	4.789343584656081
6	16	5.060582010582014
6	17	13.14565145502646
6	18	9.965443121693124
6	19	14.271122685185189
6	20	15.641319444444456
6	21	21.474305555555553
6	22	21.677058531746034
6	23	13.91376488095238
6	24	20.70181878306878
6	25	21.115426587301585
6	26	7.565343915343918
6	27	16.228000992063492
6	28	7.766517857142857
6	29	5.370205026455027
6	30	7.6100115740740755
6	31	0.14519675925925846
6	32	0.5582093253968257
6	33	1.27320601851852
6	34	1.011640211640211
6	35	1.145444775132276
6	36	1.5356729497354493
6	37	1.8169642857142847
6	38	-0.870072751322752
6	39	1.9801917989417976
6	40	2.630125661375663
7	1	6.039293981481482
7	2	10.054414682539687
7	3	9.70186838624339
7	4	7.897354497354495
7	5	12.577984457671956
7	6	12.668543320105808
7	7	11.134589947089953
7	8	12.615542328042324
7	9	10.00801091269841
7	10	15.117642195767193
7	11	8.996651785714288
7	12	11.201769179894175
7	13	13.351835317460317
7	14	11.449652777777775
7	15	14.150454695767195
7	16	10.032804232804235
7	17	11.951207010582017
7	18	10.354332010582013
7	19	10.24334490740741
7	20	13.807986111111124
7	21	10.307638888888887
7	22	15.343725198412702
7	23	17.33043154761905
7	24	16.562929894179895
7	25	14.115426587301586
7	26	3.7042328042328077
7	27	5.14466765873016
7	28	6.849851190476189
7	29	2.5090939153439162
7	30	-0.3344328703703692
7	31	-0.577025462962963
7	32	-3.9417906746031743
7	33	-4.504571759259258
7	34	-3.2105820105820113
7	35	-3.5767774470899463
7	36	-3.85321593915344
7	37	-3.3497023809523823
7	38	-3.3978505291005296
7	39	-3.7420304232804247
7	40	-3.3976521164021145
8	1	4.261516203703703
8	2	8.387748015873019
8	3	7.59075727513228
8	4	-0.8804232804232797
8	5	4.13354001322751
8	6	8.446321097883587
8	7	6.245701058201064
8	8	9.059986772486766
8	9	5.341344246031742
8	10	-2.32680224867725
8	11	4.32998511904762
8	12	1.757324735449732
8	13	9.018501984126985
8	14	4.116319444444439
8	15	-0.6273230820105873
8	16	-4.189417989417986
8	17	-5.937681878306874
8	18	-5.86789021164021
8	19	-1.3122106481481453
8	20	-2.8586805555555443
8	21	-5.025694444444445
8	22	-8.656274801587298
8	23	-0.669568452380954
8	24	-1.2148478835978835
8	25	-6.884573412698414
8	26	0.1486772486772523
8	27	-3.8553323412698406
8	28	-2.4834821428571434
8	29	-0.713128306878307
8	30	-2.2233217592592576
8	31	-2.0214699074074076
8	32	-3.9417906746031743
8	33	-1.72679398148148
8	34	1.678306878306877
8	35	-0.021221891534390025
8	36	4.369006283068783
8	37	2.6502976190476186
8	38	2.713260582010582
8	39	2.4801917989417976
8	40	2.380125661375663
12	1	5.461516203703706
12	2	11.854414682539687
12	3	5.39075727513228
12	4	12.652910052910055
12	5	13.00020667989418
12	6	12.64632109788359
12	7	11.179034391534397
12	8	14.059986772486766
12	9	10.808010912698407
12	10	10.073197751322748
12	11	11.529985119047623
12	12	11.15732473544973
12	13	12.085168650793651
12	14	13.916319444444436
12	15	7.839343584656081
12	16	15.810582010582014
12	17	15.795651455026459
12	18	16.465443121693124
12	19	6.421122685185187
12	20	18.34131944444446
12	21	15.974305555555555
12	22	11.677058531746034
12	23	15.063764880952379
12	24	10.451818783068783
12	25	9.515426587301585
12	26	8.815343915343918
12	27	6.678000992063492
12	28	8.916517857142855
12	29	6.420205026455028
12	30	4.510011574074076
12	31	2.245196759259258
12	32	0.458209325396826
12	33	-0.5267939814814806
12	34	3.2116402116402103
12	35	-0.15455522486772466
12	36	-2.7643270502645505
12	37	-0.28303571428571495
12	38	-0.8200727513227513
12	39	-0.7198082010582016
12	40	-2.4198743386243367
13	1	4.011516203703703
13	2	0.8044146825396865
13	3	-3.6592427248677204
13	4	-1.0470899470899475
13	5	-0.1997933201058224
13	6	1.1963210978835868
13	7	-0.42096560846560394
13	8	1.8099867724867664
13	9	-0.2419890873015902
13	10	1.6731977513227498
13	11	-0.1700148809523796
13	12	3.007324735449732
13	13	-0.8148313492063473
13	14	-3.6336805555555607
13	15	5.789343584656081
13	16	6.810582010582014
13	17	5.14565145502646
13	18	4.215443121693124
13	19	9.271122685185189
13	20	7.891319444444456
13	21	2.4743055555555546
13	22	0.4270585317460345
13	23	3.66376488095238
13	24	3.9518187830687825
13	25	-1.1345734126984137
13	26	-6.184656084656082
13	27	-2.0219990079365076
13	28	-5.983482142857143
13	29	-4.379794973544973
13	30	-0.6399884259259245
13	31	-0.8548032407407415
13	32	0.8082093253968257
13	33	0.5232060185185201
13	34	-4.988359788359789
13	35	0.14544477513227605
13	36	0.7856729497354493
13	37	1.0669642857142847
13	38	-0.12007275132275197
13	39	-0.019808201058202357
13	40	1.1301256613756632
15	1	-0.7384837962962969
15	2	9.054414682539687
15	3	2.5907572751322796
15	4	10.452910052910052
15	5	13.800206679894178
15	6	12.446321097883587
15	7	11.579034391534396
15	8	12.059986772486766
15	9	9.00801091269841
15	10	-7.32680224867725
15	11	7.32998511904762
15	12	8.757324735449732
15	13	13.685168650793653
15	14	4.116319444444439
15	15	2.0393435846560806
15	16	8.810582010582014
15	17	-8.60434854497354
15	18	-3.5345568783068764
15	19	4.021122685185189
15	20	-0.8586805555555443
15	21	-3.0256944444444454
15	22	15.677058531746034
15	23	19.663764880952378
15	24	16.45181878306878
15	25	-0.8845734126984137
15	26	19.815343915343917
15	27	-7.5219990079365076
15	28	-7.483482142857143
15	29	-6.379794973544973
15	30	-5.8899884259259245
15	31	-6.3548032407407415
15	32	-5.941790674603174
15	33	-5.72679398148148
15	34	-5.988359788359789
15	35	-5.354555224867724
15	36	-5.964327050264551
15	37	-5.683035714285715
15	38	-5.620072751322752
15	39	-5.519808201058202
15	40	-5.619874338624337
16	1	5.261516203703703
16	2	-4.9455853174603135
16	3	14.09075727513228
16	4	10.952910052910052
16	5	10.800206679894178
16	6	11.446321097883587
16	7	12.079034391534396
16	8	12.559986772486766
16	9	14.50801091269841
16	10	10.17319775132275
16	11	14.82998511904762
16	12	14.257324735449732
16	13	14.185168650793653
16	14	13.61631944444444
16	15	15.53934358465608
16	16	11.310582010582014
16	17	14.39565145502646
16	18	9.965443121693124
16	19	11.021122685185189
16	20	13.641319444444456
16	21	12.974305555555555
16	22	5.1770585317460345
16	23	2.66376488095238
16	24	17.45181878306878
16	25	18.115426587301585
16	26	16.815343915343917
16	27	12.478000992063492
16	28	5.016517857142857
16	29	0.12020502645502695
16	30	3.6100115740740755
16	31	-3.3548032407407415
16	32	1.5582093253968257
16	33	3.77320601851852
16	34	5.011640211640211
16	35	9.145444775132276
16	36	-5.964327050264551
16	37	-5.683035714285715
16	38	-5.620072751322752
16	39	-5.519808201058202
16	40	-5.619874338624337
17	1	4.547230489417988
17	2	6.197271825396829
17	3	5.876471560846564
17	4	8.595767195767198
17	5	6.514492394179893
17	6	7.589178240740729
17	7	8.293320105820111
17	8	8.345701058201051
17	9	7.2937251984126945
17	10	9.101769179894177
17	11	9.758556547619047
17	12	10.328753306878301
17	13	11.542311507936507
17	14	11.259176587301585
17	15	10.753629298941796
17	16	3.953439153439156
17	17	7.967080026455033
17	18	11.179728835978839
17	19	15.306836970899473
17	20	11.569890873015883
17	21	-3.311408730158732
17	22	1.9627728174603192
17	23	5.092336309523807
17	24	3.166104497354498
17	25	-2.1702876984127
17	26	3.672486772486776
17	27	0.6208581349206348
17	28	4.516517857142857
17	29	1.4773478835978846
17	30	-1.604274140211638
17	31	6.359482473544974
17	32	3.343923611111112
17	33	1.8446345899470913
17	34	0.011640211640211007
17	35	1.0740162037037049
17	36	-0.9643270502645507
17	37	-2.6830357142857153
17	38	-0.7629298941798943
17	39	1.62304894179894
17	40	-1.9055886243386224
18	1	4.261516203703703
18	2	0.9115575396825442
18	3	-1.266385582010578
18	4	1.8814814814814795
18	5	1.94306382275132
18	6	2.7320353835978715
18	7	2.7218915343915384
18	8	3.345701058201051
18	9	3.150868055555552
18	10	7.387483465608465
18	11	-1.2414434523809526
18	12	-5.242675264550268
18	13	0.1137400793650798
18	14	-3.7408234126984183
18	15	-1.3892278439153465
18	16	-9.046560846560844
18	17	-3.8900628306878264
18	18	-5.391699735449734
18	19	-0.9788773148148113
18	20	-2.715823412698402
18	21	2.5457341269841276
18	22	0.6770585317460345
18	23	1.8066220238095223
18	24	3.0232473544973555
18	25	0.8297123015872998
18	26	0.9582010582010607
18	27	2.0494295634920636
18	28	2.5165178571428566
18	29	4.048776455026456
18	30	1.6814401455026466
18	31	3.359482473544972
18	32	1.9153521825396833
18	33	4.987491732804234
18	34	4.868783068783069
18	35	1.645444775132276
18	36	0.3213872354497358
18	37	1.459821428571427
18	38	1.6656415343915345
18	39	1.0516203703703688
18	40	1.0944113756613767
19	1	5.928182870370367
19	2	15.054414682539687
19	3	8.924090608465612
19	4	12.119576719576717
19	5	12.466873346560842
19	6	13.446321097883587
19	7	12.579034391534396
19	8	14.059986772486766
19	9	11.341344246031746
19	10	19.339864417989414
19	11	15.32998511904762
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116	36	11.03567294973545
116	37	7.816964285714285
116	38	9.879927248677248
116	39	9.980191798941798
116	40	9.880125661375663
117	1	7.261516203703703
117	2	15.054414682539687
117	3	10.59075727513228
117	4	13.452910052910052
117	5	13.800206679894178
117	6	-4.553678902116413
117	7	4.579034391534396
117	8	-9.940013227513234
117	9	3.00801091269841
117	10	-4.32680224867725
117	11	-6.67001488095238
117	12	4.757324735449732
117	13	-4.314831349206347
117	14	4.116319444444439
117	15	-3.9606564153439194
117	16	6.810582010582014
117	17	0.39565145502646004
117	18	11.465443121693124
117	19	-8.978877314814811
117	20	9.141319444444456
117	21	23.974305555555553
117	22	23.677058531746034
117	23	18.663764880952378
117	24	24.45181878306878
117	25	2.1154265873015863
117	26	19.815343915343917
117	27	20.478000992063492
117	28	23.51651785714286
117	29	16.620205026455025
117	30	25.110011574074075
117	31	24.645196759259257
117	32	25.058209325396824
117	33	22.273206018518522
117	34	25.011640211640213
117	35	25.645444775132276
117	36	25.03567294973545
117	37	26.316964285714285
117	38	26.379927248677248
117	39	25.480191798941796
117	40	25.380125661375665
118	1	7.261516203703703
118	2	5.0544146825396865
118	3	6.59075727513228
118	4	13.452910052910052
118	5	11.800206679894178
118	6	8.446321097883587
118	7	-18.420965608465604
118	8	13.059986772486766
118	9	9.00801091269841
118	10	13.67319775132275
118	11	10.32998511904762
118	12	9.757324735449732
118	13	-8.314831349206347
118	14	3.1163194444444393
118	15	13.03934358465608
118	16	-10.189417989417986
118	17	10.39565145502646
118	18	-14.534556878306876
118	19	-11.978877314814811
118	20	-13.858680555555544
118	21	-10.025694444444445
118	22	-10.322941468253966
118	23	-9.33623511904762
118	24	-9.548181216931217
118	25	-8.884573412698414
118	26	-8.184656084656082
118	27	-7.5219990079365076
118	28	-7.483482142857143
118	29	-6.379794973544973
118	30	-5.8899884259259245
118	31	-6.3548032407407415
118	32	-5.941790674603174
118	33	-5.72679398148148
118	34	-5.988359788359789
118	35	-5.354555224867724
118	36	-5.964327050264551
118	37	-5.683035714285715
118	38	-5.620072751322752
118	39	-5.519808201058202
118	40	-5.619874338624337
120	1	4.261516203703703
120	2	11.054414682539687
120	3	14.59075727513228
120	4	9.452910052910052
120	5	8.300206679894178
120	6	-5.053678902116413
120	7	-15.420965608465604
120	8	-3.4400132275132336
120	9	-4.49198908730159
120	10	-0.8268022486772502
120	11	-4.67001488095238
120	12	-6.242675264550268
120	13	-15.314831349206347
120	14	-5.883680555555561
120	15	-3.9606564153439194
120	16	-15.189417989417986
120	17	-15.60434854497354
120	18	-14.534556878306876
120	19	-11.978877314814811
120	20	-13.858680555555544
120	21	-10.025694444444445
120	22	-10.322941468253966
120	23	-9.33623511904762
120	24	-9.548181216931217
120	25	-8.884573412698414
120	26	-8.184656084656082
120	27	-7.5219990079365076
120	28	-7.483482142857143
120	29	-6.379794973544973
120	30	-5.8899884259259245
120	31	-6.3548032407407415
120	32	-5.941790674603174
120	33	-5.72679398148148
120	34	-5.988359788359789
120	35	-5.354555224867724
120	36	-5.964327050264551
120	37	-5.683035714285715
120	38	-5.620072751322752
120	39	-5.519808201058202
120	40	-5.619874338624337
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	538.0
5	18.0
6	18.0
7	33.0
8	25.0
9	31.0
10	71.0
11	63.0
12	95.0
13	115.0
14	124.0
15	124.0
16	120.0
17	124.0
18	124.0
19	122.0
20	109.0
21	128.0
22	108.0
23	117.0
24	108.0
25	96.0
26	93.0
27	105.0
28	82.0
29	67.0
30	37.0
31	26.0
32	12.0
33	15.0
34	7.0
35	2.0
36	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	97.16088328075709	1.5071854188573433	0.9814230634419909	0.3505082369435682
8	95.52291010842953	2.098635886673662	0.7694998251136761	1.608954179783141
9	90.79131652661064	5.2521008403361344	2.2058823529411766	1.7507002801120448
10	39.83910458202168	35.431969220007	10.5631339629241	14.16579223504722
11	36.34137810423225	19.867086393844	26.51276670164393	17.278768800279817
12	42.49737670514166	15.459951031829311	21.895767750961877	20.146904512067156
13	25.603357817418676	21.615949632738722	26.58272123119972	26.19797131864288
14	29.415879678209162	20.42672263029031	31.584470094438615	18.57292759706191
15	31.024833857992306	26.7226302903113	25.848198670863937	16.40433718083246
16	31.514515564882828	20.146904512067156	30.080447708989155	18.25813221406086
17	31.30465197621546	23.784540048968168	23.36481287163344	21.54599510318293
18	36.72612801678909	22.73522210563134	19.06260930395243	21.47604057362714
19	18.76534549280954	21.60645387583304	17.81830936513504	41.80989126622238
20	27.70199370409234	24.379153550192374	22.560335781741866	25.358516963973415
21	22.140608604407134	24.554039874081845	31.584470094438615	21.720881427072403
22	20.146904512067156	19.237495627841902	33.997901364113325	26.617698495977614
23	29.275970619097585	23.889471843301855	26.26792584819867	20.566631689401888
24	27.87688002798181	23.784540048968168	23.22490381252186	25.113676110528154
25	20.86104305215261	27.861393069653484	22.121106055302764	29.156457822891145
26	20.232230823363828	20.548909218859958	33.60309641097818	25.615763546798032
27	23.30300909727082	18.369489153254023	24.91252624212736	33.41497550734779
28	17.3837005946135	26.51276670164393	25.113676110528154	30.98985659321441
29	22.57273045916579	22.747984577637574	27.30459165790396	27.374693305292674
30	18.962495618647036	16.158429723098493	36.83841570276902	28.040658955485455
31	22.46757798808272	16.9645986680687	37.04872064493516	23.519102698913425
32	29.730675061210217	14.760405736271423	31.619447359216508	23.889471843301855
33	19.62224554039874	20.88142707240294	37.670514165792234	21.825813221406086
34	13.711087792934592	19.097586568730325	42.46239944036376	24.72892619797132
35	18.65593279663983	21.456072803640183	37.83689184459223	22.051102555127756
36	20.266013300665033	23.836191809590478	33.07665383269163	22.821141057052852
37	24.273204903677758	20.21015761821366	32.469352014010504	23.047285464098074
38	17.418677859391394	20.741518013291362	37.81042322490381	24.02938090241343
39	19.237495627841902	24.239244491080797	31.86428821266177	24.658971668415532
40	15.250087443161945	17.733473242392446	33.29835606855544	33.71808324589017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	5.0
14	7.5
15	10.0
16	11.5
17	13.0
18	13.0
19	12.0
20	11.0
21	11.0
22	11.0
23	11.0
24	21.0
25	31.0
26	34.0
27	37.0
28	37.0
29	35.5
30	34.0
31	48.0
32	62.0
33	62.0
34	64.0
35	66.0
36	82.5
37	99.0
38	99.0
39	114.0
40	129.0
41	138.0
42	147.0
43	147.0
44	166.5
45	186.0
46	193.5
47	201.0
48	201.0
49	206.5
50	212.0
51	203.0
52	194.0
53	194.0
54	185.5
55	177.0
56	186.0
57	195.0
58	195.0
59	195.0
60	195.0
61	205.5
62	216.0
63	216.0
64	209.0
65	202.0
66	195.5
67	189.0
68	189.0
69	151.5
70	114.0
71	88.0
72	62.0
73	62.0
74	45.5
75	29.0
76	23.0
77	17.0
78	17.0
79	11.5
80	6.0
81	5.5
82	5.0
83	5.0
84	3.0
85	1.0
86	1.5
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.2098635886673662
8	0.0
9	0.1049317943336831
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.27981811822315494
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.06995452955578874
26	0.5946135012242042
27	0.03497726477789437
28	0.0
29	0.2098635886673662
30	0.2098635886673662
31	0.2098635886673662
32	0.0
33	0.0
34	0.0
35	0.06995452955578874
36	0.06995452955578874
37	0.13990905911157747
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2859.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.08884225253585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.91449814126393	91.18572927597062
2	2.0074349442379185	3.777544596012592
3	0.5576208178438662	1.5739769150052465
4	0.07434944237918216	0.27981811822315494
5	0.07434944237918216	0.3497726477789437
6	0.03717472118959108	0.2098635886673662
7	0.14869888475836432	0.9793634137810423
8	0.03717472118959108	0.27981811822315494
9	0.07434944237918216	0.6295907660020986
>10	0.07434944237918216	0.7345225603357817
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	11	0.38474991255683805	No Hit
TAAGGGGGGATGAGATGGACTTAAATGAATTGACGGGTAC	10	0.3497726477789437	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	9	0.3147953830010493	No Hit
TAAGGGGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	9	0.3147953830010493	No Hit
TAAGGGGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	8	0.27981811822315494	No Hit
TAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	7	0.24484085344526058	No Hit
TAAGGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	7	0.24484085344526058	No Hit
TAAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	7	0.24484085344526058	No Hit
TAAGGGGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	7	0.24484085344526058	No Hit
TAAGGGGGGATGAGATGGACTCAAATGAATTGACGGGTAC	6	0.2098635886673662	No Hit
TAAGGGGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	5	0.17488632388947184	No Hit
TAAGGGGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC	5	0.17488632388947184	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	4	0.13990905911157747	No Hit
TAAGGGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	4	0.13990905911157747	No Hit
TAAGGGGGGGGTTCTCTTTGAAAGATGTGTCATATCTTGT	3	0.1049317943336831	No Hit
TAAGGGGGGGCTCTTGCTTCTTGCTCTCTTTTCCTGAAGA	3	0.1049317943336831	No Hit
TAAGGGGGGCTGAGATGGACTCAAATGAATTGACGGGTAC	3	0.1049317943336831	No Hit
TAAGGGGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC	3	0.1049317943336831	No Hit
TAAGGGGGGGCTGGTGAGCTGGCTCCGTGGGTCAGAGCAC	3	0.1049317943336831	No Hit
TAAGGGGGGGGGCTGGAGAGATGGCTCCGCCGTTAAAGGC	3	0.1049317943336831	No Hit
TAAGGGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1049317943336831	No Hit
TAAGGGGGGGTCTGTAAAATGCCAGGGTTTGCCTAGATTA	3	0.1049317943336831	No Hit
TAAGGGGGGATGAGATGGCCTTCAATGAATTGACGGGTAC	3	0.1049317943336831	No Hit
TAAGGGGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	3	0.1049317943336831	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCCGTGGGTCAGAGC	3	0.1049317943336831	No Hit
TAAGGGGGGAGATGATGCATTGAGCTTGTTTGTCAAATAC	3	0.1049317943336831	No Hit
TAAGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	3	0.1049317943336831	No Hit
TAAGGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1049317943336831	No Hit
TAAGGGGGGATGAGATGGCCTCCAATGAATTGACGGGTAC	3	0.1049317943336831	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGA	25	1.5664106E-4	34.0	3
GGCTCCG	20	0.0023498372	33.999996	23
TGGCTCC	20	0.0023498372	33.999996	22
TAAGGGG	280	0.0	33.392857	1
AAGGGGG	280	0.0	32.785713	2
AGGGGGG	240	0.0	32.583336	3
GGGGATG	30	4.5729693E-4	28.333336	6
GGGGGGA	105	0.0	27.52381	4
GGATGAG	25	0.007012712	27.199999	8
GATGAGA	25	0.007012712	27.199999	9
GAATTGA	25	0.007012712	27.199999	27
GGGATGA	25	0.007012712	27.199999	7
AATTGAC	25	0.007012712	27.199999	28
ATGAGAT	25	0.007012712	27.199999	10
GGGGGAG	40	7.3849726E-5	25.499998	5
GATGGCT	35	0.0011274174	24.285713	20
ATGGCTC	35	0.0011274174	24.285713	21
GGGGAGT	35	0.0011274174	24.285713	6
GGGGGAT	55	2.2770786E-5	21.636362	5
GGGCTGG	50	3.3658475E-4	20.4	10
>>END_MODULE