FastQCFastQC Report
Fri 17 Jun 2016
SRR1526095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7289
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1812.4831938537522293No Hit
TAAGGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT290.39785978872273287No Hit
TAAGGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG190.26066675812868706No Hit
TAAGGGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT170.2332281520098779No Hit
TAAGGGGGGATTGAACAAAAACTCATCTCAGAAGAGGATC140.19207024283166416No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC130.17835093977225958No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.17835093977225958No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.164631636712855No Hit
TAAGGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA120.164631636712855No Hit
TAAGGGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT100.13719303059404583No Hit
TAAGGGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT90.12347372753464124No Hit
TAAGGGGGGATTCGTAGGAGTAAACATAACATTCTTCCCT90.12347372753464124No Hit
TAAGGGGGGGAGTCCACTTTAAATCCTTTAACGAGGATCC80.10975442447523666No Hit
TAAGGGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG80.10975442447523666No Hit
TAAGGGGGGGTCCCATTTATTTCTGAGTGAGATGGTGAGC80.10975442447523666No Hit
TAAGGGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA80.10975442447523666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT650.034.18753
GGGTACC454.1964086E-934.18757
GGGGATG200.002540332534.18756
GGTACCT454.1964086E-934.18758
ATCCTGC406.4321284E-833.95172520
GTTGATC406.4321284E-833.95172516
GGTTGAT454.50018E-933.95172515
GTACCTG454.50018E-933.9517259
CCAGTAG454.50018E-933.95172526
AGTAGCA406.4321284E-833.95172528
CCTGCCA454.50018E-933.95172522
CAGTAGC406.4321284E-833.95172527
TGATCCT359.1679976E-733.95172518
CTGGTTG454.50018E-933.95172513
ACCTGGT454.50018E-933.95172511
GATCCTG359.1679976E-733.95172519
TTGATCC359.1679976E-733.95172517
TACCTGG454.50018E-933.95172510
TCCTGCC406.4321284E-833.95172521
TGGTTGA503.1286618E-1033.9517214
Produced by FastQC (version 0.11.5)