FastQCFastQC Report
Fri 17 Jun 2016
SRR1526094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86304
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG20752.404291805710048No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT15451.7901835372636263No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC10151.1760752688172043No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG5550.643075639599555No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC3510.4067018909899888No Hit
AGTTTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT3060.35456062291434925No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT2580.29894327030033374No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2450.2838802373007045No Hit
AGTTTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2240.25954764553207266No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG2000.23173896922506487No Hit
AGTTTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1770.20508898776418244No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1760.2039302929180571No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1680.1946607341490545No Hit
AGTTTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1670.19350203930292917No Hit
AGTTTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1600.18539117538005193No Hit
AGTTTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1470.1703281423804227No Hit
AGTTTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1180.13672599184278827No Hit
AGTTTAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC1140.13209121245828698No Hit
AGTTTAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1100.1274564330737857No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1100.1274564330737857No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1040.12050426399703375No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC1020.1181868743047831No Hit
AGTTTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC990.11471078976640711No Hit
AGTTTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT980.1135520949202818No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT950.11007601038190583No Hit
AGTTTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC910.10544123099740452No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC910.10544123099740452No Hit
AGTTTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG910.10544123099740452No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC870.10080645161290322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT550.050.70254538
CGATTGA150.0079532450.7025440
GGATAGA203.884886E-450.7025440
CGGATAG203.884886E-450.7025439
GCGGATA203.884886E-450.7025438
GCGTAGA150.0079532450.7025438
AGCGTAG150.007990519550.64264337
CGAACAA150.007990519550.64264341
TTCCCCG150.007990519550.64264337
CAGCGTA150.0080654750.52327736
ATGTCTG203.9534608E-450.52327336
TCCGGAC150.0081031450.463835
CCACTAG150.00823601850.2567434
TTGGGCC402.5156623E-950.2567434
ATCCTAG204.0698866E-450.227314
CATTCAG150.008255132550.227314
TCCTAGC204.0698866E-450.227315
TCCACGA301.0044951E-650.227345
CGCAGGA150.008255132550.227315
CCACGAT301.008566E-650.1978946
Produced by FastQC (version 0.11.5)