##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526094_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 86304 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.88737486095662 38.0 35.0 40.0 30.0 40.0 2 34.926249073044126 38.0 35.0 40.0 25.0 40.0 3 35.18606321839081 38.0 35.0 40.0 26.0 40.0 4 35.04688079347423 38.0 35.0 40.0 25.0 40.0 5 34.93695541342232 38.0 35.0 40.0 25.0 40.0 6 32.21487995921394 38.0 28.0 39.0 13.0 40.0 7 34.92941230997405 38.0 35.0 40.0 26.0 40.0 8 34.8529616240267 38.0 35.0 40.0 26.0 40.0 9 34.8757763255469 38.0 35.0 40.0 25.0 40.0 10 34.329173618835746 38.0 34.0 40.0 22.0 40.0 11 33.93692065257694 38.0 33.0 39.0 22.0 40.0 12 32.930501483129405 38.0 31.0 39.0 18.0 40.0 13 32.2477868928439 38.0 29.0 39.0 15.0 40.0 14 33.03149332591769 38.0 31.0 39.0 18.0 40.0 15 31.683479328883944 37.0 28.0 39.0 12.0 40.0 16 32.578629032258064 38.0 30.0 39.0 17.0 40.0 17 31.442459213941415 36.0 28.0 39.0 13.0 40.0 18 30.96856460882462 36.0 26.0 39.0 12.0 40.0 19 32.07599879495736 37.0 29.0 39.0 16.0 40.0 20 32.80196746384872 37.0 30.0 39.0 19.0 40.0 21 33.04706618464961 38.0 31.0 39.0 18.0 40.0 22 32.28700871338524 38.0 30.0 39.0 15.0 40.0 23 33.06424962921765 38.0 31.0 39.0 18.0 40.0 24 32.390700315165 38.0 30.0 39.0 15.0 40.0 25 32.8600180756396 38.0 31.0 39.0 18.0 40.0 26 33.14954115684093 38.0 32.0 39.0 18.0 40.0 27 33.49369670003708 38.0 33.0 39.0 19.0 40.0 28 33.49242213570634 38.0 33.0 39.0 19.0 40.0 29 32.95208796811272 38.0 32.0 39.0 17.0 40.0 30 32.78615127919911 38.0 31.0 39.0 16.0 40.0 31 33.58285826844642 38.0 33.0 39.0 19.0 40.0 32 33.433108546533184 38.0 33.0 39.0 18.0 40.0 33 33.168879773822766 38.0 33.0 39.0 18.0 40.0 34 33.052280311457174 38.0 33.0 39.0 17.0 40.0 35 33.03848025583982 38.0 33.0 39.0 16.0 40.0 36 32.29932563959955 37.0 31.0 39.0 9.0 40.0 37 32.07755144605117 37.0 31.0 39.0 4.0 40.0 38 31.351559603262885 36.0 30.0 39.0 4.0 40.0 39 30.281493325917687 36.0 27.0 38.0 4.0 40.0 40 28.307436503522432 35.0 20.0 38.0 4.0 40.0 41 25.995736002966257 31.0 13.0 38.0 4.0 40.0 42 27.007068038561364 32.0 19.0 38.0 4.0 39.0 43 27.299105487578792 33.0 20.0 38.0 4.0 40.0 44 26.36150120504264 32.0 16.0 38.0 4.0 40.0 45 27.365915832406376 33.0 20.0 38.0 4.0 40.0 46 25.32043705969596 30.0 15.0 37.0 4.0 39.0 47 24.379773822766037 29.0 10.0 36.0 4.0 39.0 48 25.057471264367816 30.0 13.0 36.0 4.0 39.0 49 23.97106738969225 28.0 8.0 36.0 4.0 39.0 50 24.782246477567668 30.0 10.0 36.0 4.0 39.0 51 25.012340100111235 30.0 4.0 37.0 4.0 39.0 52 24.94740684093437 31.0 4.0 37.0 4.0 39.0 53 24.743476548016314 30.0 4.0 37.0 4.0 39.0 54 24.385312384130515 30.0 4.0 36.0 4.0 39.0 55 24.261772339636632 30.0 4.0 36.0 4.0 39.0 56 23.445784668149795 30.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.064297596039552 1 2 5.091182036544389 1 3 5.011921286458765 1 4 5.133269075664167 1 5 4.948614350349921 1 6 8.548074831434302 1 7 5.324773121804569 1 8 5.306779785700563 1 9 5.24456469215945 1 10 5.679037866202101 1 11 2.8000729657132446 1 12 -3.252350375792645 1 13 -14.902755777890352 1 14 -3.2454149296130517 1 15 3.8287646898248653 1 16 0.2078244512050631 1 17 -3.4696913398990645 1 18 -1.769168656349354 1 19 1.3003354697017002 1 20 0.740496450005935 1 21 6.319690253874306 1 22 7.6723947523987945 1 23 6.726939286568932 1 24 7.635468158688546 1 25 6.402927737728856 1 26 6.574123453347653 1 27 5.802587764943784 1 28 5.579355621537182 1 29 6.416676259146765 1 30 6.446909085343133 1 31 5.128323906075849 1 32 5.073944548836774 1 33 5.308212876583397 1 34 4.81893569962153 1 35 5.24768965105287 1 36 4.420563592133405 1 37 4.607020457951538 1 38 5.472263898058404 1 39 5.347854690357487 1 40 -8.486040899107728 1 41 2.543242685234965 1 42 0.731057302822542 1 43 -7.962093928596975 1 44 -13.539961392395444 1 45 1.0482418491539498 1 46 -3.6429393439064057 1 47 -9.747692244279897 1 48 -2.3080503064773623 1 49 -11.987756877307891 1 50 -1.0311909509371233 1 51 4.6018957233112765 1 52 4.453135189190007 1 53 4.965282010934654 1 54 4.974630221718009 1 55 4.824150338436443 1 56 7.71663308783133 2 1 3.937767875759832 2 2 5.136909746334595 2 3 5.313603978766459 2 4 5.273293114125707 2 5 4.955224927273001 2 6 8.57653855521053 2 7 5.400658174252122 2 8 5.338193684301963 2 9 5.459818188662943 2 10 6.152103862705594 2 11 5.066517458720238 2 12 3.229566145186375 2 13 4.315393260571188 2 14 5.992183409547788 2 15 6.6495688856290585 2 16 6.023383891764503 2 17 7.340459446814222 2 18 6.4705057317625325 2 19 6.887748057114287 2 20 5.885874509446495 2 21 7.380551267860319 2 22 8.012757514636554 2 23 6.761685790065439 2 24 7.777622878968266 2 25 7.033554923043543 2 26 6.6146610407602395 2 27 5.7221135516570705 2 28 5.755054922236482 2 29 6.021462098307605 2 30 6.72750349093754 2 31 5.032006161320602 2 32 5.4406949858997145 2 33 5.090446268191791 2 34 5.42279278004111 2 35 5.955786241961963 2 36 6.380900130594945 2 37 7.144006909000492 2 38 7.3943027966598045 2 39 7.377520337210633 2 40 7.134042142850312 2 41 11.208780272647552 2 42 9.298692792333028 2 43 9.316205896577852 2 44 0.7542912299821793 2 45 9.197389576426676 2 46 8.079853488261428 2 47 5.314097528447377 2 48 7.708503452263898 2 49 1.4386526506641353 2 50 8.442312108503437 2 51 9.99596259394065 2 52 9.99438300387532 2 53 10.50412597946612 2 54 9.733863176263462 2 55 10.553608380394483 2 56 10.27301420671245 3 1 4.048118518025369 3 2 5.48740322448765 3 3 5.373069336104159 3 4 5.181640086302465 3 5 5.232911779427937 3 6 8.934322615122245 3 7 5.358848919676909 3 8 5.492783775062264 3 9 5.7434565361310845 3 10 5.683969160528342 3 11 6.045141671387004 3 12 6.924899180944941 3 13 7.273385622818868 3 14 6.741675052656451 3 15 3.4608568884064255 3 16 6.887788990212158 3 17 8.145944741661218 3 18 8.653315829466248 3 19 6.903172349134323 3 20 6.574660900360541 3 21 7.437487239689908 3 22 8.44200911410092 3 23 7.322019073802974 3 24 7.653087839539609 3 25 6.9510771171614785 3 26 6.225830013631338 3 27 5.995683952887045 3 28 5.574922997423705 3 29 6.224854450636126 3 30 6.470845255555897 3 31 5.042718852884363 3 32 4.957532758056633 3 33 5.0960009971507745 3 34 4.501947667706634 3 35 5.006721122684073 3 36 5.714779017665322 3 37 5.802887035965721 3 38 7.414141114370462 3 39 7.705844495322026 3 40 8.801303959048298 3 41 12.132005983107305 3 42 10.185068827645239 3 43 10.639967241615789 3 44 11.373048359377602 3 45 10.370826069012107 3 46 11.893075727015578 3 47 12.339242596145638 3 48 12.002953682884339 3 49 12.907134523401329 3 50 11.670105591616071 3 51 11.39129067011979 3 52 11.30381116436731 3 53 11.36892784426799 3 54 10.867970203987511 3 55 11.057749629216584 3 56 10.707324577193031 4 1 4.083420730367912 4 2 5.539059835051852 4 3 5.360102256608016 4 4 4.8243838144701385 4 5 5.2215854324394755 4 6 8.651269794120868 4 7 5.4601509203120315 4 8 5.461980345402054 4 9 5.478240065293775 4 10 5.819779470679713 4 11 6.349629868698319 4 12 6.8653035421178 4 13 7.3440959012141285 4 14 7.496306152476503 4 15 7.767197525645763 4 16 7.306705048219989 4 17 8.591059592936759 4 18 9.037617724247664 4 19 7.412275768209163 4 20 6.953299621647723 4 21 7.4262667464116205 4 22 7.929624230010738 4 23 7.21341316716595 4 24 7.184208830330334 4 25 6.997493969072142 4 26 6.4750096473775045 4 27 5.420474891809459 4 28 4.9842063678058395 4 29 5.547972900937811 4 30 5.874147891313282 4 31 4.523030062792266 4 32 4.294676824956181 4 33 4.504808025837129 4 34 4.4916269309648165 4 35 4.2230861062767495 4 36 5.446916204073702 4 37 5.610868405712733 4 38 6.227044681640496 4 39 6.618726891850024 4 40 0.696561712832569 4 41 10.531815446428993 4 42 9.562913646106125 4 43 9.446254952000043 4 44 10.721465846410528 4 45 8.679072073034543 4 46 11.441715506839863 4 47 11.944355330346966 4 48 10.99453831292562 4 49 11.559934354035395 4 50 11.51719990727183 4 51 10.02085561137098 4 52 10.239399181727322 4 53 10.52066913780033 4 54 10.08528787097174 4 55 9.981799592167786 4 56 9.788461446040284 5 1 4.1651904531824115 5 2 5.27711953654439 5 3 5.3070105721730485 5 4 5.320545861378456 5 5 5.028301850349919 5 6 8.638476617148587 5 7 5.183924907518858 5 8 5.139592285700566 5 9 5.256171835016595 5 10 6.0556003662021 5 11 6.094939037141817 5 12 7.294301409921644 5 13 7.691217436395359 5 14 7.260165427529802 5 15 7.903764689824868 5 16 7.247110165490774 5 17 8.353746160100933 5 18 9.146679557936363 5 19 7.907478326844554 5 20 6.86214823572022 5 21 6.930850968160023 5 22 7.943376895255938 5 23 7.072475000854649 5 24 7.557343158688546 5 25 6.655383094871713 5 26 5.534391310490513 5 27 5.456828836372356 5 28 5.147212764394325 5 29 6.046586973432479 5 30 6.0647662282002734 5 31 4.676761406075848 5 32 5.38934633455106 5 33 5.220266448011969 5 34 5.313801771050098 5 35 5.672020008195727 5 36 6.37815287784769 5 37 6.885815100808681 5 38 7.488558540915545 5 39 8.316381476071776 5 40 9.8206555294637 5 41 11.899939113806397 5 42 11.29489658853683 5 43 11.548173928545882 5 44 11.624770750461696 5 45 11.270116849153947 5 46 12.111971370379308 5 47 12.362798827148673 5 48 12.592619336379784 5 49 12.946394908406393 5 50 12.661442977634309 5 51 11.625333223311276 5 52 12.079697689190006 5 53 12.057023082363227 5 54 12.279317721718009 5 55 12.347364624150732 5 56 10.761275944974187 6 1 4.177131565505199 6 2 4.827882895323015 6 3 5.308319187222125 6 4 5.231014781771037 6 5 4.855401086991144 6 6 8.635205079525907 6 7 5.329126652338921 6 8 5.092264041425757 6 9 5.46713148605258 6 10 5.747203419637216 6 11 6.539159320675079 6 12 7.75057185703178 6 13 8.166244699208885 6 14 7.435465318478553 6 15 8.285825758527153 6 16 7.430152695479869 6 17 8.83237211429941 6 18 9.093844225330034 6 19 7.941556843747499 6 20 7.028724026341813 6 21 7.221527085935378 6 22 7.700901431788111 6 23 6.937578599546033 6 24 8.003908044184726 6 25 7.0657974896372515 6 26 6.240988911362919 6 27 6.328530035936151 6 28 5.507790736041002 6 29 6.365507366016995 6 30 6.566183894503435 6 31 5.688975146533863 6 32 4.648670216775702 6 33 4.660669937652102 6 34 5.353823581300919 6 35 5.612968754106301 6 36 6.3615225615990525 6 37 6.773110629707265 6 38 7.209441856073671 6 39 8.46730841173153 6 40 4.893610818449524 6 41 12.590833334089929 6 42 10.961854058547736 6 43 10.735305880563327 6 44 11.59756246256639 6 45 11.393780971291353 6 46 12.125548251513443 6 47 12.560454225185751 6 48 12.365792727873782 6 49 13.441214973837145 6 50 12.279818113948373 6 51 12.04518055155555 6 52 11.137712956365583 6 53 11.80658687734687 6 54 12.462523828588239 6 55 12.145238124695982 6 56 11.093064385541254 7 1 4.158047596039552 7 2 5.388057036544389 7 3 5.433796286458765 7 4 5.430144075664167 7 5 5.104864350349921 7 6 8.993387331434302 7 7 5.168523121804569 7 8 5.533342285700563 7 9 5.86956469215945 7 10 5.889975366202101 7 11 6.565697965713245 7 12 7.075774624207355 7 13 7.940994222109648 7 14 7.184272570386948 7 15 8.266264689824865 7 16 7.739074451205063 7 17 8.725621160100935 7 18 9.441768843650646 7 19 8.0893979697017 7 20 7.154558950005935 7 21 7.671252753874306 7 22 8.414582252398795 7 23 7.492564286568932 7 24 8.487030658688546 7 25 7.043552737728856 7 26 6.988185953347653 7 27 6.654150264943784 7 28 6.290293121537182 7 29 7.088551259146765 7 30 7.001596585343133 7 31 5.643948906075849 7 32 5.745819548836774 7 33 5.964462876583397 7 34 5.32674819962153 7 35 5.68518965105287 7 36 6.928376092133405 7 37 7.403895457951538 7 38 7.612888898058404 7 39 8.918167190357487 7 40 7.748334100892272 7 41 12.496367685234965 7 42 12.809182302822542 7 43 11.717593571403025 7 44 12.327226107604556 7 45 10.84511684915395 7 46 13.099248156093594 7 47 13.111682755720103 7 48 12.816949693522638 7 49 13.496618122692109 7 50 12.453184049062877 7 51 12.617520723311276 7 52 12.578135189190007 7 53 12.668407010934654 7 54 12.709005221718009 7 55 12.699150338436443 7 56 12.21663308783133 8 1 4.6661776773403645 8 2 5.9921601869508905 8 3 5.930239375889663 8 4 6.035644583794252 8 5 5.9433425007564225 8 6 9.15764038021479 8 7 5.555527694975304 8 8 5.687559765375362 8 9 5.751932578338312 8 10 6.427958089779338 8 11 6.967819408802672 8 12 8.062245660792719 8 13 8.6224601773942 8 14 8.03107135087475 8 15 9.039130543483402 8 16 8.420222825188802 8 17 9.509030712946462 8 18 10.184019859910808 8 19 8.739359859945601 8 20 8.041881104477483 8 21 8.155183140053168 8 22 8.94970674426871 8 23 8.071197416650232 8 24 8.610188687143832 8 25 7.7384841401678806 8 26 6.815866339526515 8 27 6.329709208033211 8 28 6.462485702837995 8 29 7.2310817469516415 8 30 7.395079817050448 8 31 6.634484983311623 8 32 6.154038552901813 8 33 6.3329842180468106 8 34 6.5090398662881945 8 35 6.572194224223605 8 36 7.671706884816331 8 37 7.932795356325524 8 38 8.60545749561938 8 39 9.336358247268059 8 40 5.30346621471341 8 41 13.401855490113014 8 42 12.362027831277828 8 43 11.9137952990453 8 44 12.733539623864718 8 45 12.34003554834094 8 46 13.727360452841559 8 47 14.163321475232301 8 48 14.173339429294998 8 49 14.426940785293734 8 50 14.411326833615725 8 51 13.324520215181192 8 52 13.834169233905456 8 53 13.637220527194817 8 54 13.580130729848094 8 55 13.91002432217628 8 56 12.617547721977669 9 1 4.532063344071048 9 2 5.706647095599507 9 3 5.7315325069312095 9 4 5.404122914246841 9 5 5.319246732239684 9 6 9.163785953481547 9 7 5.548998023379369 9 8 5.467151340818674 9 9 5.82158240869488 9 10 6.545364145729657 9 11 7.042014402721119 9 12 7.964492636018377 9 13 8.406852981952166 9 14 7.814439893221593 9 15 8.508882800061087 9 16 8.094635474827108 9 17 9.778155608919832 9 18 9.55089778065852 9 19 8.237343343717448 9 20 7.846118989376016 9 21 7.783888088519973 9 22 8.42374809885549 9 23 7.809370388931139 9 24 8.364429772861776 9 25 7.7419410251304335 9 26 6.806776012402771 9 27 6.2700459342351245 9 28 5.635457983741908 9 29 6.98545086544598 9 30 7.03057050266597 9 31 5.221643295839627 9 32 5.031191103954885 9 33 5.809074097055841 9 34 5.456546427967989 9 35 6.171963765226099 9 36 6.881808670873561 9 37 6.641776855589331 9 38 7.48389037443636 9 39 8.411769552562212 9 40 4.414918746561565 9 41 11.43239130728221 9 42 11.58083584612963 9 43 11.13670036274161 9 44 11.596480536738415 9 45 10.840195589311431 9 46 12.712252585227453 9 47 13.143732460444511 9 48 12.867085028168304 9 49 12.977609756550375 9 50 12.46554871441721 9 51 11.672577317799469 9 52 12.1285165868278 9 53 12.712759865265365 9 54 12.539160241403046 9 55 12.724248763633295 9 56 11.177755135075426 10 1 3.8597282683084586 10 2 5.422917855872122 10 3 5.259951748643637 10 4 5.457520651294416 10 5 5.374100169677654 10 6 8.98301443227464 10 7 5.470059361300365 10 8 5.435390605028296 10 9 5.274239061907352 10 10 5.874087761160084 10 11 6.510485255629209 10 12 7.477494687232564 10 13 7.760124999420572 10 14 6.926131814084425 10 15 8.411747883102173 10 16 7.582824451205063 10 17 8.961309185311016 10 18 9.6832341797851 10 19 8.17428504953363 10 20 7.091139832358877 10 21 7.358161892529765 10 22 7.788334878449213 10 23 7.022895168921874 10 24 7.746418788940645 10 25 6.566727632686838 10 26 6.119685428137572 10 27 4.922795222926975 10 28 5.714859823217857 10 29 5.947007141499707 10 30 5.8597242113935515 10 31 4.9994504817060985 10 32 5.142287511021646 10 33 4.900518548852304 10 34 4.430608493739175 10 35 5.335465386346986 10 36 6.691178087931725 10 37 6.411445352909521 10 38 6.962087952680253 10 39 7.037389879433114 10 40 9.974437042068743 10 41 11.764224828092111 10 42 11.061283143158676 10 43 11.60027476888202 10 44 11.442837977352458 10 45 10.7474277735237 10 46 12.029198261135612 10 47 12.738849247316743 10 48 11.685056311169696 10 49 12.59555457227194 10 50 11.975308523852796 10 51 11.490879441798672 10 52 11.452806932887484 10 53 11.170048292447259 10 54 11.153267301549938 10 55 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33 3.881764705851687 115 34 4.4521292971825055 115 35 3.596584468126043 115 36 4.63512151896267 115 37 5.266128689658853 115 38 6.032286763912065 115 39 6.242862312308709 115 40 8.445742637477636 115 41 9.04819695352765 115 42 7.918938400383517 115 43 7.320299364085951 115 44 -1.0632083436149564 115 45 6.762799775983218 115 46 3.211100290239937 115 47 -8.019605354035994 115 48 2.230249998400687 115 49 -2.3576120602347217 115 50 3.2121398417458025 115 51 4.539967369652739 115 52 4.228478176994884 115 53 4.6453506084956295 115 54 3.98663479488874 115 55 4.487260094534005 115 56 7.837059917099623 116 1 4.608047596039555 116 2 5.897119536544388 116 3 5.151296286458766 116 4 5.544831575664169 116 5 5.708301850349919 116 6 8.842762331434304 116 7 5.188210621804572 116 8 5.0895922857005615 116 9 5.093314692159453 116 10 6.135600366202098 116 11 5.223510465713243 116 12 5.468587124207353 116 13 6.326931722109645 116 14 6.473022570386949 116 15 -2.4462353101751333 116 16 5.832824451205063 116 17 7.373746160100936 116 18 7.102393843650649 116 19 6.840335469701699 116 20 6.116433950005934 116 21 7.046565253874306 116 22 7.640519752398795 116 23 6.778189286568935 116 24 7.307343158688546 116 25 6.798240237728859 116 26 6.287248453347651 116 27 5.645400264943785 116 28 5.374355621537184 116 29 5.702301259146768 116 30 6.031909085343134 116 31 4.996761406075848 116 32 5.353632048836776 116 33 5.8288378765834 116 34 5.352373199621528 116 35 5.044877151052869 116 36 6.852438592133403 116 37 7.092957957951537 116 38 8.145701398058403 116 39 8.310667190357485 116 40 9.972084100892275 116 41 10.291367685234967 116 42 7.659182302822543 116 43 2.1810310714030265 116 44 1.661913607604557 116 45 7.13011684915395 116 46 3.1276856560935933 116 47 -4.128629744279898 116 48 2.139762193522639 116 49 0.240680622692107 116 50 3.322871549062878 116 51 7.575333223311276 116 52 6.969697689190006 116 53 7.555594510934654 116 54 6.009317721718009 116 55 7.761650338436443 116 56 9.104133087831329 117 1 3.631731806565867 117 2 4.848172168123334 117 3 4.39761207593245 117 4 4.537463154611537 117 5 4.641459745086763 117 6 8.155393910381672 117 7 4.6508422007519385 117 8 4.6501186014900355 117 9 4.673841007948923 117 10 5.392442471465259 117 11 3.0735104657132446 117 12 -4.449307612634751 117 13 -6.078331435785088 117 14 1.7188120440711607 117 15 -5.533077415438292 117 16 -5.8777018645844095 117 17 1.566904054837778 117 18 -2.5623429984546178 117 19 0.6819144170701215 117 20 0.6422234236901438 117 21 5.734986306505888 117 22 6.539993436609322 117 23 5.626610339200514 117 24 6.109974737635916 117 25 5.677187606149911 117 26 6.01409055861081 117 27 5.606452896522729 117 28 5.44119772680034 117 29 5.695459153883608 117 30 5.795593295869448 117 31 4.895182458707431 117 32 4.793105733047302 117 33 5.2646273502676095 117 34 5.473952146989948 117 35 4.91750873000024 117 36 6.447701750028145 117 37 6.798221115846278 117 38 7.022017187532089 117 39 8.046456664041699 117 40 9.72366304826069 117 41 9.437157158919177 117 42 7.782866513348857 117 43 -3.9958110338601323 117 44 -0.6312442871322865 117 45 8.141169480732895 117 46 1.2608435508304368 117 47 -0.8007350074377904 117 48 1.1997621935226377 117 49 -1.0498456930973639 117 50 3.238134706957613 117 51 8.112175328574434 117 52 8.719171373400535 117 53 8.338226089882024 117 54 9.437212458560115 117 55 7.722176654225915 117 56 10.259396245726066 118 1 3.3663809293728875 118 2 4.593786203211053 118 3 4.121296286458765 118 4 4.359831575664167 118 5 4.433857405905478 118 6 7.699984553656524 118 7 4.500988399582347 118 8 4.481258952367227 118 9 4.6299813588261145 118 10 4.322267032868766 118 11 1.9346215768243589 118 12 -4.013635098014866 118 13 -2.9314016112236843 118 14 -6.180310762946384 118 15 -9.712901976841803 118 16 -8.153286659906048 118 17 -0.8490316176768431 118 18 -5.0148283785715755 118 19 -0.03299786363163193 118 20 -0.6041216055496221 118 21 4.78323192054097 118 22 6.0005197523987945 118 23 4.994300397680046 118 24 5.265676492021882 118 25 4.38990690439552 118 26 4.943915120014317 118 27 4.310400264943784 118 28 4.468244510426068 118 29 5.4184123702578795 118 30 5.391353529787576 118 31 3.401761406075849 118 32 3.4758542710589957 118 33 3.087726765472283 118 34 3.411817644065973 118 35 2.8093215954973125 118 36 5.384105258800069 118 37 4.859624624618203 118 38 5.75959028694729 118 39 6.980667190357487 118 40 8.203195212003386 118 41 8.399145463012744 118 42 6.864737858378099 118 43 2.435475515847468 118 44 2.701358052048999 118 45 6.8312279602650605 118 46 1.233796767204705 118 47 -1.1947408553910073 118 48 -0.38357113981069446 118 49 1.2579028449143301 118 50 3.9245382157295445 118 51 8.000333223311276 118 52 7.193586578078897 118 53 6.646705622045765 118 54 7.848762166162452 118 55 7.469983671769775 118 56 11.181910865609108 119 1 3.34040053721602 119 2 4.621237183603213 119 3 4.021296286458764 119 4 4.217184516840639 119 5 4.5488900856440395 119 6 8.070409390257833 119 7 4.507034151216331 119 8 4.492533462171153 119 9 4.469197045100628 119 10 5.149718013260923 119 11 2.20292223041913 119 12 -5.203177581674996 119 13 0.22399054563905807 119 14 -4.282271547260109 119 15 -7.969764721939839 119 16 -8.252469666441996 119 17 -0.46390089872259566 119 18 -5.2529002739964135 119 19 0.06504135205464223 119 20 -0.6500366382293592 119 21 5.17832995975666 119 22 5.909343281810557 119 23 4.740542227745404 119 24 4.410284335159133 119 25 5.301181414199444 119 26 5.82724845334765 119 27 5.400106147296722 119 28 4.866120327419537 119 29 5.864654200323233 119 30 5.663085555931371 119 31 4.841467288428788 119 32 4.868337931189718 119 33 5.192955523642219 119 34 5.242373199621529 119 35 5.31193597458228 119 36 6.760085650956931 119 37 6.68707560501036 119 38 7.207466103940757 119 39 8.48949071976925 119 40 9.515613512656977 119 41 8.056661802882022 119 42 6.444476420469602 119 43 -2.5677924580087392 119 44 -1.6680863923954448 119 45 5.823058025624537 119 46 -0.41113787331817164 119 47 -2.7233356266328386 119 48 -4.197296630006775 119 49 -1.859907612602008 119 50 1.388753902004055 119 51 7.425333223311277 119 52 7.291462395072362 119 53 7.482653334464064 119 54 6.52637654524742 119 55 7.852826809024677 119 56 8.577662499596034 120 1 3.417083740617862 120 2 4.8909749582311335 120 3 4.440573394892503 120 4 4.686638804579829 120 5 4.649386187699321 120 6 7.485292451916234 120 7 4.51086122421421 120 8 4.469712767628273 120 9 4.481386981316078 120 10 5.2821063902984875 120 11 2.1337514295686653 120 12 -3.128762273383007 120 13 -2.247164663432521 120 14 -2.4614352609383516 120 15 2.3453309548851067 120 16 -8.236452657228671 120 17 1.0537461601009355 120 18 -3.538088084060199 120 19 1.0955161925932657 120 20 0.940891781331235 120 21 4.679818265922499 120 22 6.057748668061443 120 23 5.487104949219532 120 24 5.442885327363243 120 25 4.25185469556018 120 26 5.304356887082591 120 27 4.604074963738963 120 28 4.73146405527212 120 29 5.435313307339538 120 30 5.321909085343133 120 31 3.312905984389104 120 32 3.4554392777524363 120 33 3.891608960920749 120 34 3.986951512874544 120 35 3.949214500450463 120 36 4.444848230687619 120 37 4.289343500120214 120 38 4.3571471811909355 120 39 6.049944298791225 120 40 7.477626269566969 120 41 -4.209957615969856 120 42 -2.263106853803965 120 43 -6.496920735825892 120 44 -12.060857476732794 120 45 -0.7828951990388227 120 46 -6.089663741496768 120 47 -5.356220105725679 120 48 -3.405659493224352 120 49 -13.78209046164524 120 50 -1.4303814629853164 120 51 3.167501898010073 120 52 2.739938653045428 120 53 3.818727041055137 120 54 3.978112902440902 120 55 3.391168410725598 120 56 6.696301762530126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4164.0 5 94.0 6 155.0 7 297.0 8 225.0 9 173.0 10 162.0 11 151.0 12 169.0 13 168.0 14 245.0 15 256.0 16 344.0 17 533.0 18 594.0 19 811.0 20 1044.0 21 1291.0 22 1622.0 23 1785.0 24 2109.0 25 2377.0 26 2547.0 27 2673.0 28 2744.0 29 2980.0 30 3235.0 31 3542.0 32 4121.0 33 4782.0 34 5170.0 35 5687.0 36 6274.0 37 7210.0 38 7868.0 39 8701.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.75203930292919 0.17264553207267333 0.05329996292176493 0.022015202076381163 8 99.67208935854653 0.22826288468668893 0.07879124953652206 0.020856507230255842 9 98.6674854871789 0.42640464410275425 0.5619735119289017 0.3441363567894511 10 43.80445865776789 25.072997775305893 23.33959028550241 7.782953281423804 11 28.899008157211714 28.004495736002966 17.0525120504264 26.043984056358916 12 26.90141824249166 25.783277715980716 17.94238969225065 29.372914349276975 13 21.165878754171302 14.183583611420097 20.82985724879496 43.82068038561365 14 20.681544308490913 32.2198275862069 25.39627363737486 21.702354467927325 15 39.06255457371381 17.14691534816574 21.174018837390708 22.616511240729746 16 29.375347801892044 30.21586904099425 21.133602300129848 19.275180856983862 17 24.93480604072739 17.272632445150148 38.682908172135235 19.10965334198723 18 21.512328513162775 18.33402855024101 26.84927697441602 33.3043659621802 19 26.85400943669646 19.909806512943575 20.27266719994435 32.96351685041561 20 22.102722958940983 26.384973257689136 31.630178784820114 19.882124998549767 21 21.28662966524918 19.05032270025376 27.65836645307811 32.00468118141895 22 19.44869299221357 18.308537263626253 34.30200222469411 27.940767519466075 23 19.172923618835743 21.310715609936967 32.19433629959214 27.32202447163515 24 18.147581635727363 21.49991888572157 27.508169366613362 32.8443301119377 25 26.946253505458085 21.091848795976546 31.36486893642663 20.597028762138734 26 35.36524571918801 20.89105811702239 18.97816988766129 24.765526276128313 27 19.890758543911122 31.713652862659604 18.649905486425997 29.745683107003284 28 26.589729328883944 31.58486281053022 20.113783833889507 21.71162402669633 29 34.68686516919148 26.436821637936035 18.855418190260608 20.020895002611873 30 27.936879695761064 20.874686949262593 32.02740933123087 19.16102402374548 31 30.025610420312194 31.27947805731635 21.05501025575655 17.639901266614906 32 34.340484220530115 26.994888912068426 20.08854583173974 18.57608103566172 33 30.082027125801996 25.542109965077557 20.005569026928566 24.370293882191877 34 22.017858594303362 26.596300553871878 32.587870554220224 18.797970297604532 35 21.358017526550984 22.720677848064536 39.030816551564044 16.89048807382044 36 27.487217539506787 26.25592746750762 27.149830146896846 19.10702484608874 37 27.336041787579802 20.86012768427162 33.08763784097504 18.716192687173535 38 33.215248095149605 22.174084742612898 26.092965991451404 18.5177011707861 39 27.67527675276753 21.82459560444661 20.562092413376966 29.9380352294089 40 22.233395696913004 17.481290926099156 30.36716557530402 29.918147801683816 41 20.10526809368626 15.453778163317939 27.65573347712939 36.78522026586641 42 25.37247518769031 26.68688840852567 21.921995211863422 26.0186411919206 43 18.256301788557415 23.7207572605669 32.16149313747138 25.861447813404304 44 19.648738928525706 25.699787008694237 26.24332219881516 28.408151863964896 45 24.35367354997561 20.84852848946598 33.49864114654712 21.29915681401129 46 18.68983957219251 27.750523134154847 22.973727040223206 30.585910253429432 47 16.52055464356816 27.92660635907466 23.13925465404631 32.413584343310866 48 25.423217847038636 26.096887899448078 23.523955289643336 24.95593896386995 49 18.479574290096203 28.91783241158154 21.785100153367104 30.81749314495515 50 24.34776550907381 26.876589222891777 22.874359956807968 25.901285311226445 51 19.1632567655538 30.18576211289873 25.460336650237142 25.190644471310332 52 20.103314179580938 32.07498984270706 27.944744326426374 19.876951651285623 53 20.12921367898767 29.96200281202431 28.370071694999943 21.53871181398808 54 20.15495771768423 27.214013567512314 28.338444382492334 24.292584332311122 55 18.997815274485195 26.69664853809325 28.598986659229304 25.70654952819226 56 23.953347892755914 21.20603131824729 30.902374424980252 23.938246364016543 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.5 2 3.0 3 4.0 4 4.0 5 3.0 6 33.0 7 63.0 8 88.0 9 113.0 10 133.5 11 154.0 12 148.0 13 152.5 14 163.0 15 175.5 16 188.0 17 187.0 18 186.0 19 203.0 20 220.0 21 227.5 22 295.5 23 356.0 24 423.5 25 491.0 26 517.5 27 544.0 28 618.0 29 768.0 30 844.0 31 1043.0 32 1242.0 33 1323.5 34 1405.0 35 1409.5 36 1414.0 37 1628.5 38 2145.0 39 2447.0 40 3317.5 41 4188.0 42 6759.5 43 9331.0 44 9058.5 45 8786.0 46 7325.5 47 5577.0 48 5289.0 49 5173.5 50 5058.0 51 5139.0 52 5220.0 53 4866.0 54 4619.0 55 4726.0 56 4669.0 57 4612.0 58 4240.5 59 3869.0 60 3425.5 61 2982.0 62 2841.5 63 2247.5 64 1794.0 65 1608.5 66 1423.0 67 1240.5 68 1058.0 69 971.0 70 884.0 71 656.5 72 333.0 73 237.0 74 213.5 75 190.0 76 243.0 77 296.0 78 185.5 79 48.5 80 22.0 81 12.0 82 2.0 83 1.0 84 0.0 85 1.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0011586948461253244 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.47622358175750834 16 0.05561735261401557 17 0.026649981460882464 18 0.0 19 0.052141268075639596 20 0.128615127919911 21 0.0011586948461253244 22 0.0 23 0.0 24 0.006952169076751946 25 0.011586948461253245 26 0.054458657767890245 27 0.08458472376714868 28 0.0 29 0.18423248053392657 30 0.06488691138301816 31 0.012745643307378568 32 0.02433259176863181 33 0.13093251761216168 34 0.21204115684093439 35 0.17264553207267333 36 0.06141082684464219 37 0.17843900630329998 38 0.2410085279940675 39 0.1459955506117909 40 0.9084167593622544 41 1.1563774564330738 42 0.29894327030033374 43 0.2977845754542084 44 0.4460975157582499 45 0.23405635891731555 46 0.3290693362995921 47 0.47622358175750834 48 0.06952169076751946 49 0.27345198368557655 50 0.20508898776418244 51 0.3244345569150908 52 0.18423248053392657 53 0.2838802373007045 54 0.25027808676307006 55 0.2919911012235818 56 0.2549128661475714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 86304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.5357341490545 #Duplication Level Percentage of deduplicated Percentage of total 1 88.52027933058181 58.897617723396365 2 6.734235410898072 8.961345939933258 3 1.8337599916409801 3.6603170189099 4 0.8794385524963864 2.3405635891731555 5 0.45452170732981556 1.5120967741935485 6 0.2681852219493931 1.0706340378197998 7 0.22464865994462152 1.046301446051168 8 0.14976577329641433 0.7971820541342233 9 0.12538529857374223 0.7508342602892102 >10 0.7174825418386361 8.611420096403412 >50 0.05398533688591679 2.5873655913978495 >100 0.03134632464343556 3.750695216907675 >500 0.0017414624801908643 0.643075639599555 >1k 0.005224387440572593 5.370550611790879 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 2075 2.404291805710048 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 1545 1.7901835372636263 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 1015 1.1760752688172043 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 555 0.643075639599555 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 351 0.4067018909899888 No Hit AGTTTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 306 0.35456062291434925 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 258 0.29894327030033374 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 245 0.2838802373007045 No Hit AGTTTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 224 0.25954764553207266 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 200 0.23173896922506487 No Hit AGTTTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 177 0.20508898776418244 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 176 0.2039302929180571 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 168 0.1946607341490545 No Hit AGTTTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 167 0.19350203930292917 No Hit AGTTTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 160 0.18539117538005193 No Hit AGTTTAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 147 0.1703281423804227 No Hit AGTTTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 118 0.13672599184278827 No Hit AGTTTAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 114 0.13209121245828698 No Hit AGTTTAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 110 0.1274564330737857 No Hit AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 110 0.1274564330737857 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 104 0.12050426399703375 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 102 0.1181868743047831 No Hit AGTTTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC 99 0.11471078976640711 No Hit AGTTTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 98 0.1135520949202818 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 95 0.11007601038190583 No Hit AGTTTAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 91 0.10544123099740452 No Hit AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 91 0.10544123099740452 No Hit AGTTTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 91 0.10544123099740452 No Hit AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 87 0.10080645161290322 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0011586948461253244 0.0 0.0 0.0 0.0 7 0.0011586948461253244 0.0 0.0 0.0 0.0 8 0.0011586948461253244 0.0 0.0 0.0 0.0 9 0.0011586948461253244 0.0 0.0 0.0 0.0 10 0.012745643307378568 0.0 0.0 0.0 0.0 11 0.022015202076381163 0.0 0.0 0.0 0.0 12 0.025491286614757136 0.0 0.0 0.0 0.0 13 0.027808676307007785 0.0 0.0 0.0 0.0 14 0.054458657767890245 0.0 0.0 0.0 0.0 15 0.05561735261401557 0.0 0.0 0.0 0.0 16 0.05909343715239155 0.0 0.0 0.0 0.0 17 0.060252131998516874 0.0 0.0 0.0 0.0 18 0.06256952169076752 0.0 0.0 0.0 0.0 19 0.06604560622914349 0.0 0.0 0.0 0.0 20 0.07183908045977011 0.0 0.0 0.0 0.0 21 0.07415647015202076 0.0 0.0 0.0 0.0 22 0.07531516499814608 0.0 0.0 0.0 0.0 23 0.08458472376714868 0.0 0.0 0.0 0.0 24 0.08921950315164998 0.0 0.0 0.0 0.0 25 0.09385428253615127 0.0 0.0 0.0 0.0 26 0.09848906192065257 0.0 0.0 0.0 0.0 27 0.10775862068965517 0.0 0.0 0.0 0.0 28 0.11702817945865777 0.0 0.0 0.0 0.0 29 0.11934556915090842 0.0 0.0 0.0 0.0 30 0.12513904338153503 0.0 0.0 0.0 0.0 31 0.13672599184278827 0.0 0.0 0.0 0.0 32 0.13904338153503892 0.0 0.0 0.0 0.0 33 0.14136077122728957 0.0 0.0 0.0 0.0 34 0.1425194660734149 0.0 0.0 0.0 0.0 35 0.14483685576566555 0.0 0.0 0.0 0.0 36 0.1471542454579162 0.0 0.0 0.0 0.0 37 0.15063032999629217 0.0 0.0 0.0 0.0 38 0.1517890248424175 0.0 0.0 0.0 0.0 39 0.1517890248424175 0.0 0.0 0.0 0.0 40 0.1517890248424175 0.0 0.0 0.0 0.0 41 0.15526510938079346 0.0 0.0 0.0 0.0 42 0.16337597330367074 0.0 0.0 0.0 0.0 43 0.17496292176492398 0.0 0.0 0.0 0.0 44 0.1900259547645532 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCATT 55 0.0 50.702545 38 CGATTGA 15 0.00795324 50.70254 40 GGATAGA 20 3.884886E-4 50.70254 40 CGGATAG 20 3.884886E-4 50.70254 39 GCGGATA 20 3.884886E-4 50.70254 38 GCGTAGA 15 0.00795324 50.70254 38 AGCGTAG 15 0.0079905195 50.642643 37 CGAACAA 15 0.0079905195 50.642643 41 TTCCCCG 15 0.0079905195 50.642643 37 CAGCGTA 15 0.00806547 50.523277 36 ATGTCTG 20 3.9534608E-4 50.523273 36 TCCGGAC 15 0.00810314 50.4638 35 CCACTAG 15 0.008236018 50.25674 34 TTGGGCC 40 2.5156623E-9 50.25674 34 ATCCTAG 20 4.0698866E-4 50.2273 14 CATTCAG 15 0.0082551325 50.2273 14 TCCTAGC 20 4.0698866E-4 50.2273 15 TCCACGA 30 1.0044951E-6 50.2273 45 CGCAGGA 15 0.0082551325 50.2273 15 CCACGAT 30 1.008566E-6 50.19789 46 >>END_MODULE