FastQCFastQC Report
Fri 17 Jun 2016
SRR1526093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6927
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT721.0394110004330879No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT260.375342861267504No Hit
AGTTTAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA160.23098022231846396No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT160.23098022231846396No Hit
AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT150.21654395842356No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT130.187671430633752No Hit
AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT130.187671430633752No Hit
AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT130.187671430633752No Hit
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT120.17323516673884798No Hit
AGTTTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA110.158798902843944No Hit
AGTTTAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT100.14436263894904No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.12992637505413598No Hit
AGTTTAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT80.11549011115923198No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT70.10105384726432798No Hit
AGTTTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10105384726432798No Hit
AGTTTAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA70.10105384726432798No Hit
AGTTTAGGGGATCCAGTGACAGATACCATTAGATTCAAGA70.10105384726432798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG503.0377123E-1034.0000046
AGGGGAT251.8275414E-434.0000046
AGGGCTC251.8275414E-434.0000046
AGGGCAG251.8275414E-434.0000046
AGGGATG251.8275414E-434.0000046
AGGGAAT251.8275414E-434.0000046
TAGGGTG251.8275414E-434.0000045
TAGGGTA503.0377123E-1034.0000045
TAGGGAC251.8275414E-434.0000045
AGGGGTG200.002602144434.06
GTTGATC200.002602144434.016
AGGGGGC358.979532E-734.06
AGGGGCC301.2821087E-534.06
TGGTTGA200.002602144434.014
GGTTGAT200.002602144434.015
GTACCTG301.2821087E-534.09
GGGTACC301.2821087E-534.07
TGATCCT200.002602144434.018
AGGGTGG200.002602144434.06
AGGGTAC301.2821087E-534.06
Produced by FastQC (version 0.11.5)