##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526093_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6927 Sequences flagged as poor quality 0 Sequence length 40 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.237187815793273 29.0 21.0 33.0 4.0 35.0 2 21.582647610798325 24.0 11.0 31.0 4.0 35.0 3 17.379673740435976 15.0 12.0 22.0 4.0 35.0 4 20.380973004186515 21.0 11.0 30.0 4.0 35.0 5 21.711563447379817 25.0 10.0 32.0 4.0 35.0 6 20.817236899090517 23.0 9.0 31.0 4.0 35.0 7 19.080698715172513 19.0 9.0 29.0 4.0 33.0 8 21.080554352533564 24.0 10.0 31.0 4.0 35.0 9 18.200519705500216 18.0 4.0 28.0 4.0 33.0 10 19.53702901689043 21.0 4.0 30.0 4.0 35.0 11 21.319907607911073 23.0 4.0 33.0 4.0 37.0 12 20.150714595062798 22.0 4.0 32.0 4.0 36.0 13 20.34892449833983 23.0 4.0 32.0 4.0 36.0 14 20.358019344593618 23.0 4.0 33.0 4.0 36.0 15 19.92002309802223 22.0 4.0 33.0 4.0 36.0 16 18.024541648621337 18.0 4.0 30.0 4.0 35.0 17 18.1053847264328 18.0 4.0 31.0 4.0 35.0 18 18.056157066551176 18.0 4.0 31.0 4.0 35.0 19 16.53082142341562 14.0 4.0 29.0 4.0 35.0 20 17.00274289014003 15.0 4.0 30.0 4.0 35.0 21 15.675472787642558 11.0 4.0 29.0 4.0 34.0 22 14.835282228959146 9.0 4.0 27.0 4.0 33.0 23 15.012415186949617 4.0 4.0 28.0 4.0 33.0 24 14.630576006929406 4.0 4.0 28.0 4.0 33.0 25 14.24974736538184 4.0 4.0 28.0 4.0 33.0 26 12.356286992926231 4.0 4.0 23.0 4.0 33.0 27 12.296520860401328 4.0 4.0 23.0 4.0 33.0 28 12.095279341706366 4.0 4.0 23.0 4.0 33.0 29 11.836148404792839 4.0 4.0 22.0 4.0 33.0 30 11.28872527789808 4.0 4.0 19.0 4.0 32.0 31 10.422116356286994 4.0 4.0 16.0 4.0 31.0 32 10.04951638515952 4.0 4.0 12.0 4.0 31.0 33 9.37736393821279 4.0 4.0 4.0 4.0 30.0 34 8.759058755594053 4.0 4.0 4.0 4.0 28.0 35 8.358741157788364 4.0 4.0 4.0 4.0 27.0 36 8.171791540349357 4.0 4.0 4.0 4.0 27.0 37 8.137866320196332 4.0 4.0 4.0 4.0 27.0 38 7.680958567922621 4.0 4.0 4.0 4.0 23.0 39 7.66623357874982 4.0 4.0 4.0 4.0 24.0 40 7.594485347192147 4.0 4.0 4.0 4.0 24.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.6562228713315648 1 2 10.812312747638838 1 3 13.952175276414408 1 4 10.192954111106296 1 5 8.518973261038468 1 6 6.492540442649144 1 7 13.662136872789041 1 8 4.863131301066087 1 9 0.5593987413552668 1 10 8.574782837935015 1 11 4.322254581167623 1 12 5.569852719744031 1 13 0.5476002258610961 1 14 1.4972727514031838 1 15 4.735054764076505 1 16 -2.9548697196523293 1 17 -3.6519847785065185 1 18 -3.075465573074263 1 19 0.6872509374683293 1 20 1.7585054920924463 1 21 5.259059418842028 1 22 3.033670677148937 1 23 2.366331953071086 1 24 0.0035133948177357155 1 25 -3.0600787497526625 1 26 -1.7906662057749028 1 27 -1.457059896625113 1 28 -0.009626038212994459 1 29 -1.119067212980255 1 30 -1.4094165858296286 1 31 -2.1590339853383353 1 32 -1.1197152485195971 1 33 -1.2938978292239174 1 34 -1.5636824045519697 1 35 -2.307817206947644 1 36 -0.8084081136255064 1 37 0.6273684286727752 1 38 -1.09714171094606 1 39 -0.43536867721650374 1 40 -0.924568921416749 2 1 0.7990800141887071 2 2 -5.973401538075446 2 3 16.880746704985835 2 4 13.97866839682058 2 5 -15.623883881818674 2 6 -14.29317384306514 2 7 -13.123577412925243 2 8 -14.851154413219628 2 9 -12.29774411578759 2 10 -12.425217162064985 2 11 -14.320602561689519 2 12 -13.144432994541685 2 13 -13.809542631281762 2 14 -13.145584391453959 2 15 -13.122088093066353 2 16 -11.5262982910809 2 17 -11.29484192136366 2 18 -10.86117985878855 2 19 -10.384177633960242 2 20 -10.527208793621838 2 21 -9.312369152586545 2 22 -8.680615037136777 2 23 -8.9193823326432 2 24 -8.710772319467978 2 25 -8.060078749752662 2 26 -6.790666205774903 2 27 -6.242774182339399 2 28 -6.0096260382129945 2 29 -5.976210070123113 2 30 -5.837988014401057 2 31 -5.016176842481192 2 32 -4.691143819948168 2 33 -4.365326400652489 2 34 -3.777968118837684 2 35 -3.2363886355190727 2 36 -3.4512652564826496 2 37 -3.5869172856129383 2 38 -3.0257131395174888 2 39 -2.9353686772165037 2 40 -2.99599749284532 3 1 5.799080014188707 3 2 -15.973401538075446 3 3 -12.119253295014165 3 4 -15.02133160317942 3 5 -15.623883881818674 3 6 -14.29317384306514 3 7 -13.123577412925243 3 8 -14.851154413219628 3 9 -12.29774411578759 3 10 -12.425217162064985 3 11 -14.320602561689519 3 12 -13.144432994541685 3 13 -13.809542631281762 3 14 -13.145584391453959 3 15 -13.122088093066353 3 16 -11.5262982910809 3 17 -11.29484192136366 3 18 -10.86117985878855 3 19 -10.384177633960242 3 20 -10.527208793621838 3 21 -9.312369152586545 3 22 -8.680615037136777 3 23 -8.9193823326432 3 24 -8.710772319467978 3 25 -8.060078749752662 3 26 -6.790666205774903 3 27 -6.242774182339399 3 28 -6.0096260382129945 3 29 -5.976210070123113 3 30 -5.837988014401057 3 31 -5.016176842481192 3 32 -4.691143819948168 3 33 -4.365326400652489 3 34 -3.777968118837684 3 35 -3.2363886355190727 3 36 -3.4512652564826496 3 37 -3.5869172856129383 3 38 -3.0257131395174888 3 39 -2.9353686772165037 3 40 -2.99599749284532 5 1 7.0718072869159805 5 2 2.299325734651827 5 3 15.244383068622199 5 4 9.433213851366034 5 5 -1.8057020636368577 5 6 -7.293173843065141 5 7 -6.487213776561607 5 8 -7.94206350412872 5 9 -6.297744115787591 5 10 -4.788853525701349 5 11 -7.502420743507701 5 12 -8.68988753999623 5 13 -9.445906267645398 5 14 -6.6001298459994135 5 15 -6.758451729429989 5 16 -3.708116472899082 5 17 -4.3857510122727525 5 18 -5.133907131515823 5 19 -6.20235945214206 5 20 -3.0726633390763833 5 21 0.051267211049818684 5 22 -1.7715241280458685 5 23 -2.5557459690068374 5 24 -1.8016814103770695 5 25 -2.696442386116299 5 26 -0.5179389330476294 5 27 0.9390439994787823 5 28 0.8085557799688239 5 29 2.0237899298768873 5 30 -0.6561698325828758 5 31 2.0747322484278996 5 32 0.21794708914273997 5 33 0.1801281448020564 5 34 1.7674864266168608 5 35 2.945429546299109 5 36 0.6396438344264421 5 37 1.776719078023425 5 38 -2.1166222304265796 5 39 -0.29900504085286705 5 40 -2.99599749284532 6 1 3.37050858561728 6 2 6.026598461924554 6 3 1.8807467049858353 6 4 6.550096968249154 6 5 5.518973261038468 6 6 11.135397585506286 6 7 8.162136872789041 6 8 3.1488455867803715 6 9 11.416541598498124 6 10 7.431925695077872 6 11 6.250826009739054 6 12 8.284138434029742 6 13 9.476171654432523 6 14 11.997272751403184 6 15 8.306483335505074 6 16 5.330844566061957 6 17 8.990872364350624 6 18 14.853105855497166 6 19 9.615822366039758 6 20 10.32993406352102 6 21 9.544773704556313 6 22 10.176527820006081 6 23 13.366331953071084 6 24 7.717799109103449 6 25 7.08277839310448 6 26 13.637905222796524 6 27 12.328654389089174 6 28 9.561802533215578 6 29 10.738075644162603 6 30 13.876297699884658 6 31 11.698108871804523 6 32 13.023141894337547 6 33 11.206102170776084 6 34 10.222031881162316 6 35 10.3350399359095 6 36 6.120163314945922 6 37 7.984511285815633 6 38 12.831429717625369 6 39 11.064631322783496 6 40 12.861145364297538 7 1 4.0213022364109285 7 2 12.248820684146775 7 3 7.880746704985835 7 4 12.089779507931691 7 5 12.265005007070215 7 6 13.70682615693486 7 7 11.876422587074757 7 8 7.371067809002593 7 9 12.368922550879077 7 10 18.019227282379457 7 11 10.56828632719937 7 12 14.300011449902758 7 13 14.190457368718238 7 14 14.63219338632382 7 15 16.766800795822533 7 16 7.918146153363542 7 17 12.482935856414118 7 18 9.472153474544783 7 19 8.504711254928647 7 20 8.917235650822604 7 21 6.576519736302345 7 22 8.430496073974334 7 23 7.4139510006901315 7 24 6.067005458309801 7 25 7.828810139136227 7 26 3.542667127558431 7 27 0.979448039882822 7 28 4.768151739564784 7 29 2.9126788187657766 7 30 -0.9490991255121681 7 31 2.7616009352965865 7 32 2.197745068940721 7 33 1.079118043791956 7 34 -0.22241256328212877 7 35 -0.5697219688524058 7 36 0.993179187961795 7 37 2.635304936609283 7 38 1.6409535271491773 7 39 1.175742433894607 7 40 1.5595580627102352 8 1 -4.83728362217493 8 2 -7.064310628984538 8 3 -2.6647078404687097 8 4 1.1604865786387641 8 5 -3.8966111545459476 8 6 -4.020446570337867 8 7 -6.214486503834335 8 8 -7.214790776855992 8 9 -6.297744115787591 8 10 -2.516126252974077 8 11 -1.0478752889622456 8 12 -0.7807966309053214 8 13 -0.08226990400903489 8 14 2.672597426727858 8 15 -0.5766335476118059 8 16 1.8373380725554629 8 17 4.886976260454523 8 18 5.22972923212054 8 19 6.161276911494305 8 20 2.654609388196345 8 21 5.778539938322545 8 22 4.13756678104504 8 23 -0.283018696279564 8 24 1.2892276805320222 8 25 1.0308303411564292 8 26 1.7547883396796422 8 27 3.4844985449333272 8 28 3.717646689059732 8 29 0.9328808389677956 8 30 1.434739258326216 8 31 0.3474595211551712 8 32 1.12703799823365 8 33 1.2710372357111481 8 34 1.3129409720714076 8 35 1.7636113644809273 8 36 -0.26944707466446793 8 37 0.5039918052961534 8 38 1.8833777695734195 8 39 3.8828131409653146 8 40 2.367638870791043 9 1 1.799080014188707 9 2 6.33429076961686 9 3 -0.4269456027064731 9 4 -0.09825468010249594 9 5 5.453039195104402 9 6 9.70682615693486 9 7 4.953345663997833 9 8 3.841153279088065 9 9 4.933025114981639 9 10 9.882475145627321 9 11 8.833243592156634 9 12 6.778643928535239 9 13 6.498149676410545 9 14 8.700569454699888 9 15 7.262527291549031 9 16 3.781394016611406 9 17 5.782081155559416 9 18 10.67728167967299 9 19 6.077360827578218 9 20 8.088175821762778 9 21 10.456861616644225 9 22 9.62707727055553 9 23 13.003694590433723 9 24 11.135381526685869 9 25 9.786075096401184 9 26 6.132410717302021 9 27 7.372610433045217 9 28 5.913450884863929 9 29 7.177636083723041 9 30 5.3927812163681725 9 31 4.829977003672655 9 32 4.924240795436447 9 33 1.9423659070398198 9 34 5.222031881162316 9 35 6.225149826019389 9 36 3.7795039742865804 9 37 4.72077502207937 9 38 1.9742868604825112 9 39 1.4492467073988813 9 40 3.3116948148469882 10 1 8.986580014188707 10 2 14.901598461924554 10 3 18.443246704985835 10 4 9.16616839682058 10 5 15.563616118181326 10 6 13.89432615693486 10 7 12.688922587074757 10 8 14.148845586780372 10 9 12.76475588421241 10 10 15.449782837935015 10 11 12.116897438310481 10 12 15.730567005458315 10 13 12.877957368718238 10 14 11.416915608546041 10 15 14.627911906933647 10 16 11.0987017089191 10 17 17.017658078636337 10 18 14.45132014121145 10 19 18.615822366039758 10 20 15.722791206378162 10 21 13.937630847413455 10 22 18.381884962863225 10 23 17.643117667356798 10 24 17.476727680532022 10 25 18.627421250247338 10 26 14.959333794225097 10 27 14.5072258176606 10 28 16.302873961787007 10 29 16.461289929876887 10 30 11.349511985598943 10 31 13.733823157518808 10 32 11.371356180051832 10 33 11.384673599347511 10 34 9.472031881162316 10 35 8.326111364480926 10 36 9.48623474351735 10 37 10.413082714387063 10 38 8.099286860482511 10 39 8.252131322783496 10 40 9.62900250715468 11 1 -4.000919985811294 11 2 -0.17340153807544567 11 3 -4.419253295014165 11 4 3.7786683968205814 11 5 -2.523883881818673 11 6 1.50682615693486 11 7 -1.6235774129252434 11 8 0.44884558678037223 11 9 -2.797744115787591 11 10 1.674782837935016 11 11 7.179397438310481 11 12 2.8555670054583153 11 13 3.490457368718239 11 14 3.454415608546043 11 15 2.0779119069336467 11 16 1.2737017089191003 11 17 3.9051580786363385 11 18 2.738820141211452 11 19 4.815822366039757 11 20 4.37279120637816 11 21 3.487630847413456 11 22 4.5193849628632226 11 23 5.180617667356801 11 24 7.089227680532023 11 25 1.7399212502473382 11 26 0.8093337942250969 11 27 -0.7427741823393994 11 28 1.4903739617870055 11 29 1.2237899298768866 11 30 2.062011985598943 11 31 1.2838231575188086 11 32 2.1088561800518324 11 33 -2.465326400652488 11 34 0.022031881162315692 11 35 0.8636113644809269 11 36 1.7487347435173497 11 37 1.0130827143870613 11 38 -0.8257131395174886 11 39 -0.7353686772165036 11 40 -1.0959974928453198 12 1 6.0213022364109285 12 2 5.582154017480111 12 3 6.102968927208057 12 4 10.089779507931691 12 5 10.265005007070215 12 6 10.262381712490416 12 7 5.098644809296978 12 8 10.259956697891482 12 9 5.257811439767966 12 10 10.574782837935015 12 11 15.45717521608826 12 12 10.744455894347205 12 13 8.30156847982935 12 14 13.187748941879374 12 15 17.10013412915587 12 16 9.251479486696878 12 17 12.482935856414118 12 18 11.472153474544783 12 19 14.393600143817537 12 20 14.25056898415594 12 21 12.465408625191234 12 22 15.097162740641002 12 23 17.74728433402347 12 24 16.84478323608758 12 25 19.606587916914005 12 26 9.987111572002876 12 27 11.423892484327268 12 28 7.323707295120338 12 29 7.801567707654666 12 30 6.6064564300433855 12 31 3.317156490852142 12 32 2.0866339578296103 12 33 4.968006932680845 12 34 3.55536521449565 12 35 1.4302780311475933 12 36 4.104290299072906 12 37 3.7464160477203956 12 38 3.6409535271491773 12 39 5.509075767227941 12 40 4.670669173821346 13 1 -1.075919985811293 13 2 -0.2859015380754464 13 3 -4.619253295014165 13 4 -2.1463316031794193 13 5 -1.9988838818186743 13 6 1.7068261569348593 13 7 -3.8110774129252434 13 8 -2.4136544132196285 13 9 -3.485244115787591 13 10 0.012282837935014612 13 11 6.366897438310481 13 12 4.668067005458315 13 13 2.3779573687182385 13 14 3.6669156085460415 13 15 2.8154119069336474 13 16 2.7237017089190996 13 17 6.642658078636339 13 18 4.2013201412114505 13 19 6.990822366039758 13 20 9.160291206378162 13 21 5.937630847413455 13 22 8.256884962863223 13 23 7.643117667356799 13 24 8.476727680532022 13 25 9.814921250247338 13 26 4.271833794225097 13 27 6.132225817660601 13 28 1.4903739617870055 13 29 6.711289929876887 13 30 3.6620119855989426 13 31 0.9838231575188079 13 32 -0.9411438199481683 13 33 -0.8028264006524886 13 34 -1.2779681188376841 13 35 -0.5488886355190727 13 36 -0.5137652564826496 13 37 -0.4619172856129383 13 38 -0.08821313951748877 13 39 -0.8103686772165037 13 40 0.31650250715467987 15 1 5.260618475727167 15 2 7.949675385001477 15 3 -0.5807917565526264 15 4 8.97866839682058 15 5 6.683808425873632 15 6 7.245287695396399 15 7 1.7225764332286033 15 8 1.6103840483188314 15 9 1.3945635765201025 15 10 4.344013607165785 15 11 12.525551284464328 15 12 7.932490082381392 15 13 12.651995830256698 15 14 11.315954070084501 15 15 11.493296522318264 15 16 12.089086324303716 15 17 13.24361961709788 15 18 13.061897064288374 15 19 6.5388992891166815 15 20 15.165098898685855 15 21 11.533784693567302 15 22 8.780923424401683 15 23 12.157540744279876 15 24 13.827689218993562 15 25 15.555305865631954 15 26 13.13241071730202 15 27 11.449533509968294 15 28 16.759604731017774 15 29 17.485328391415347 15 30 11.700473524060483 15 31 12.214592388288038 15 32 15.770394641590292 15 33 11.634673599347511 15 34 14.222031881162316 15 35 10.840534441404003 15 36 11.54873474351735 15 37 12.413082714387063 15 38 10.743517629713281 15 39 7.833862092014266 15 40 8.542464045616217 16 1 -2.3120310969224036 16 2 -5.6400682047421125 16 3 -4.008142183903054 16 4 -7.465776047623864 16 5 -1.4016616595964528 16 6 2.7068261569348593 16 7 -2.4569107462585773 16 8 -2.628932190997407 16 9 -2.5199663380098123 16 10 3.0192272823794575 16 11 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-3.777968118837684 117 35 -3.2363886355190727 117 36 -3.4512652564826496 117 37 -3.5869172856129383 117 38 -3.0257131395174888 117 39 -2.9353686772165037 117 40 -2.99599749284532 118 1 8.132413347522043 118 2 15.693265128591218 118 3 16.21408003831917 118 4 16.97866839682058 118 5 13.709449451514661 118 6 9.70682615693486 118 7 1.5430892537414245 118 8 11.148845586780372 118 9 10.035589217545741 118 10 -3.7585504953983193 118 11 -6.987269228356185 118 12 -4.144432994541685 118 13 -4.476209297948428 118 14 -11.145584391453959 118 15 -11.455421426399685 118 16 -2.8596316244142344 118 17 -5.628175254696995 118 18 -0.5278465254552156 118 19 -0.05084430062690792 118 20 -0.19387546028850444 118 21 -0.9790358192532107 118 22 0.9860516295298893 118 23 -0.5860489993098668 118 24 1.9558943471986883 118 25 1.2732545835806715 118 26 2.2093337942250972 118 27 -6.242774182339399 118 28 -6.0096260382129945 118 29 -5.976210070123113 118 30 -5.837988014401057 118 31 -5.016176842481192 118 32 -4.691143819948168 118 33 -4.365326400652489 118 34 -3.777968118837684 118 35 -3.2363886355190727 118 36 -3.4512652564826496 118 37 -3.5869172856129383 118 38 -3.0257131395174888 118 39 -2.9353686772165037 118 40 -2.99599749284532 119 1 1.799080014188707 119 2 10.026598461924554 119 3 3.8807467049858353 119 4 10.97866839682058 119 5 -15.623883881818674 119 6 -14.29317384306514 119 7 -13.123577412925243 119 8 -14.851154413219628 119 9 -12.29774411578759 119 10 -12.425217162064985 119 11 -14.320602561689519 119 12 -13.144432994541685 119 13 -13.809542631281762 119 14 -13.145584391453959 119 15 -13.122088093066353 119 16 -11.5262982910809 119 17 -11.29484192136366 119 18 -10.86117985878855 119 19 -10.384177633960242 119 20 -10.527208793621838 119 21 -9.312369152586545 119 22 -8.680615037136777 119 23 -8.9193823326432 119 24 -8.710772319467978 119 25 -8.060078749752662 119 26 -6.790666205774903 119 27 -6.242774182339399 119 28 -6.0096260382129945 119 29 -5.976210070123113 119 30 -5.837988014401057 119 31 -5.016176842481192 119 32 -4.691143819948168 119 33 -4.365326400652489 119 34 -3.777968118837684 119 35 -3.2363886355190727 119 36 -3.4512652564826496 119 37 -3.5869172856129383 119 38 -3.0257131395174888 119 39 -2.9353686772165037 119 40 -2.99599749284532 120 1 10.799080014188707 120 2 15.026598461924554 120 3 20.5474133716525 120 4 17.645335063487245 120 5 15.709449451514661 120 6 4.040159490268191 120 7 -0.7902440795919112 120 8 3.4821789201137037 120 9 7.368922550879077 120 10 6.908116171268347 120 11 6.012730771643813 120 12 9.855567005458315 120 13 -4.142875964615095 120 14 -4.478917724787292 120 15 -3.4554214263996865 120 16 -11.5262982910809 120 17 -11.29484192136366 120 18 -10.86117985878855 120 19 -10.384177633960242 120 20 -10.527208793621838 120 21 -9.312369152586545 120 22 -8.680615037136777 120 23 -8.9193823326432 120 24 -8.710772319467978 120 25 -8.060078749752662 120 26 -6.790666205774903 120 27 -6.242774182339399 120 28 -6.0096260382129945 120 29 -5.976210070123113 120 30 -5.837988014401057 120 31 -5.016176842481192 120 32 -4.691143819948168 120 33 -4.365326400652489 120 34 -3.777968118837684 120 35 -3.2363886355190727 120 36 -3.4512652564826496 120 37 -3.5869172856129383 120 38 -3.0257131395174888 120 39 -2.9353686772165037 120 40 -2.99599749284532 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1326.0 5 112.0 6 114.0 7 108.0 8 124.0 9 178.0 10 197.0 11 239.0 12 237.0 13 251.0 14 285.0 15 269.0 16 297.0 17 281.0 18 325.0 19 325.0 20 327.0 21 328.0 22 293.0 23 279.0 24 250.0 25 206.0 26 182.0 27 142.0 28 85.0 29 70.0 30 45.0 31 22.0 32 18.0 33 6.0 34 3.0 35 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 98.75686614628505 0.5059265683723619 0.5492917028042786 0.18791558253830587 8 98.1521582214523 0.563014291901256 0.57745055579616 0.7073769308502958 9 97.76011560693641 1.1705202312138727 0.476878612716763 0.5924855491329479 10 36.269130811435176 36.24025411492926 13.817499278082588 13.67311579555299 11 26.08632885809153 36.119532265049806 20.672729897502524 17.121408979356143 12 35.81637072325682 16.03868918723834 20.87483759203118 27.27010249747365 13 26.230691497040564 18.940378230114046 24.541648621336797 30.28728165150859 14 22.08748375920312 24.5560848852317 31.34112891583658 22.015302439728597 15 29.608777248448103 22.85260574563303 26.14407391367114 21.394543092247726 16 29.9696838458207 19.57557384148982 27.400028872527788 23.054713440161688 17 26.172946441460947 22.101920023098025 27.616572830951352 24.10856070448968 18 26.73596073336221 22.679370578894183 26.14407391367114 24.44059477407247 19 22.738457084961645 17.1225937183384 18.04892169633811 42.09002750036185 20 21.683268370145807 28.713728886964052 22.982532120687164 26.620470622202973 21 21.149126606034358 21.495596939512055 29.9696838458207 27.385592608632887 22 21.322361772773206 18.680525480005773 23.401183773639385 36.595928973581636 23 25.220153024397284 21.235744189403782 26.043020066406815 27.50108271979212 24 20.6468380017326 22.581576667629225 29.58417557031476 27.187409760323415 25 26.870206916509915 23.585588192736218 24.395890609173783 25.14831428158009 26 19.790910410919125 22.172208508784667 27.41396834615943 30.622912734136783 27 18.879098656651742 19.890221002455583 28.325870287447643 32.90481005344504 28 19.36741767764298 25.10109763142692 28.74061236279607 26.790872328134025 29 22.20133063349725 19.771478160254556 31.313277408157365 26.713913798090832 30 17.160618765360706 18.76536070550817 37.38615006505711 26.68787046407402 31 17.347826086956523 24.463768115942027 36.347826086956516 21.84057971014493 32 24.065251912804968 17.381261729464413 35.484336653674035 23.069149704056592 33 16.601703479139598 19.05586834127328 37.27443337664212 27.067994802945 34 14.161974880900823 22.56388046773495 40.638082864154754 22.636061787209467 35 15.896621426508808 20.603522956973723 42.650880739243426 20.84897487727404 36 14.929252093560496 24.790643950332083 38.13167773606699 22.148426220040427 37 14.64084405260876 20.59546177193236 43.64792600086717 21.115768174591704 38 18.507290313266928 22.08748375920312 38.48707954381406 20.918146383715893 39 16.272018481085766 21.065550101068435 38.53595148714987 24.12647993069593 40 13.988739714161975 23.38674750974448 41.99509167027573 20.629421105817812 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 0.5 10 0.0 11 4.0 12 8.0 13 8.0 14 15.0 15 22.0 16 28.0 17 34.0 18 34.0 19 54.0 20 74.0 21 79.0 22 84.0 23 84.0 24 99.5 25 115.0 26 145.0 27 175.0 28 175.0 29 212.0 30 249.0 31 292.0 32 335.0 33 335.0 34 364.0 35 393.0 36 416.5 37 440.0 38 440.0 39 457.0 40 474.0 41 473.0 42 472.0 43 472.0 44 437.0 45 402.0 46 457.0 47 512.0 48 512.0 49 516.0 50 520.0 51 501.5 52 483.0 53 483.0 54 490.5 55 498.0 56 472.5 57 447.0 58 447.0 59 420.0 60 393.0 61 363.5 62 334.0 63 334.0 64 267.5 65 201.0 66 159.0 67 117.0 68 117.0 69 97.5 70 78.0 71 60.5 72 43.0 73 43.0 74 27.5 75 12.0 76 9.5 77 7.0 78 7.0 79 5.5 80 4.0 81 2.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.12992637505413598 8 0.0 9 0.10105384726432798 10 0.014436263894903998 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.25985275010827197 20 0.0 21 0.0 22 0.0 23 0.0 24 0.014436263894903998 25 0.23098022231846396 26 0.57745055579616 27 0.05774505557961599 28 0.043308791684711995 29 0.187671430633752 30 0.14436263894904 31 0.3897791251624079 32 0.0 33 0.0 34 0.0 35 0.014436263894903998 36 0.014436263894903998 37 0.11549011115923198 38 0.0 39 0.014436263894903998 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 6927.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.65973725999712 #Duplication Level Percentage of deduplicated Percentage of total 1 95.46178343949045 86.54540204994947 2 3.0254777070063694 5.485780280063519 3 0.7802547770700637 2.122130792550888 4 0.17515923566878983 0.635195611375776 5 0.14331210191082802 0.64963187527068 6 0.14331210191082802 0.7795582503248158 7 0.06369426751592357 0.4042153890573119 8 0.01592356687898089 0.11549011115923198 9 0.01592356687898089 0.12992637505413598 >10 0.15923566878980894 2.09325826476108 >50 0.01592356687898089 1.0394110004330879 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 72 1.0394110004330879 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT 26 0.375342861267504 No Hit AGTTTAGGGCTCGTCTTAATCAGCAACATCTTTTGATACA 16 0.23098022231846396 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT 16 0.23098022231846396 No Hit AGTTTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 15 0.21654395842356 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT 13 0.187671430633752 No Hit AGTTTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 13 0.187671430633752 No Hit AGTTTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 13 0.187671430633752 No Hit AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.17323516673884798 No Hit AGTTTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA 11 0.158798902843944 No Hit AGTTTAGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT 10 0.14436263894904 No Hit AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 9 0.12992637505413598 No Hit AGTTTAGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT 8 0.11549011115923198 No Hit AGTTTAGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT 7 0.10105384726432798 No Hit AGTTTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10105384726432798 No Hit AGTTTAGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA 7 0.10105384726432798 No Hit AGTTTAGGGGATCCAGTGACAGATACCATTAGATTCAAGA 7 0.10105384726432798 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGG 50 3.0377123E-10 34.000004 6 AGGGGAT 25 1.8275414E-4 34.000004 6 AGGGCTC 25 1.8275414E-4 34.000004 6 AGGGCAG 25 1.8275414E-4 34.000004 6 AGGGATG 25 1.8275414E-4 34.000004 6 AGGGAAT 25 1.8275414E-4 34.000004 6 TAGGGTG 25 1.8275414E-4 34.000004 5 TAGGGTA 50 3.0377123E-10 34.000004 5 TAGGGAC 25 1.8275414E-4 34.000004 5 AGGGGTG 20 0.0026021444 34.0 6 GTTGATC 20 0.0026021444 34.0 16 AGGGGGC 35 8.979532E-7 34.0 6 AGGGGCC 30 1.2821087E-5 34.0 6 TGGTTGA 20 0.0026021444 34.0 14 GGTTGAT 20 0.0026021444 34.0 15 GTACCTG 30 1.2821087E-5 34.0 9 GGGTACC 30 1.2821087E-5 34.0 7 TGATCCT 20 0.0026021444 34.0 18 AGGGTGG 20 0.0026021444 34.0 6 AGGGTAC 30 1.2821087E-5 34.0 6 >>END_MODULE