FastQCFastQC Report
Fri 17 Jun 2016
SRR1526092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062
Sequences flagged as poor quality0
Sequence length40
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.6591337099811676No Hit
AGTTTAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT50.4708097928436911No Hit
AGTTTAGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3766478342749529No Hit
AGTTTAGGGCCGAGTCGCCTTTTTTTTTTTTTTTTTTTTT30.2824858757062147No Hit
AGTTTAGGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2824858757062147No Hit
AGTTTAGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT30.2824858757062147No Hit
AGTTTAGGGTCAACGCAGAGTCGATTTTTTTTTTTTTTTT20.18832391713747645No Hit
AGTTTAGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT20.18832391713747645No Hit
AGTTTAGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18832391713747645No Hit
AGTTTAGGGTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18832391713747645No Hit
AGTTTAGGGAGCAGTCTTTTTTTTTTTTTTTTTTTTTTTT20.18832391713747645No Hit
AGTTTAGGGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18832391713747645No Hit
AGTTTAGGGGTCTCTCTTGCTTTTTCGCTCTCTTGCTTTT20.18832391713747645No Hit
AGTTTAGGGACCTTTTTTTTTTTTTTTTTTTTTTCCTTTT20.18832391713747645No Hit
AGTTTAGGGGCCGGTAGTGGTGGCCCACCCCCGTAGGATT20.18832391713747645No Hit
AGTTTAGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAA20.18832391713747645No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG20.18832391713747645No Hit
AGTTTAGGGGTTTGAAGGTAACTGGCAACTGAAATGTAGA20.18832391713747645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGT401.29530235E-833.6666684
TAGGGTG200.001792092933.6666685
TAGGGTC200.001792092933.6666685
TTAGGGA305.4420852E-633.6666644
GTTTAGG1100.030.6060582
TTTAGGG1100.030.6060583
AGTTTAG1100.030.6060581
Produced by FastQC (version 0.11.5)