FastQCFastQC Report
Fri 17 Jun 2016
SRR1526091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6031
Sequences flagged as poor quality0
Sequence length40
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1322.1886917592439064No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT1282.1223677665395457No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG941.5586138285524789No Hit
AGTTTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT230.38136295805007464No Hit
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC180.29845796716962364No Hit
AGTTTAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT160.2652959708174432No Hit
AGTTTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG160.2652959708174432No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG130.2155529762891726No Hit
AGTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.2155529762891726No Hit
AGTTTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT120.19897197811308243No Hit
AGTTTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG120.19897197811308243No Hit
AGTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.18239097993699221No Hit
AGTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.18239097993699221No Hit
AGTTTAGGGGGGGAGGTAGTGACGAAAAATAACAATACAG110.18239097993699221No Hit
AGTTTAGGGGTGCAGTGCTAGTTTAGGGTCACAGATCCTC110.18239097993699221No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA100.165809981760902No Hit
AGTTTAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT100.165809981760902No Hit
AGTTTAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT90.14922898358481182No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC90.14922898358481182No Hit
AGTTTAGGGTACATGGTTGATCCTGCCAGTAGCATATGCT90.14922898358481182No Hit
AGTTTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT80.1326479854087216No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAGATGCGTTTTTGT70.11606698723263141No Hit
AGTTTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT70.11606698723263141No Hit
AGTTTAGGGTCACAGATCCTCTTCTGAAGGACATTGGGCG70.11606698723263141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTT251.7985818E-434.06
AGGGGGG502.8558134E-1034.06
AGGGATT358.71325E-734.06
AGGGAGA251.7985818E-434.06
TAGGGTA251.7985818E-434.05
TAGGGGT358.71325E-734.05
TAGGGGG950.034.05
TAGGGGC358.71325E-734.05
TAGGGAG454.1600288E-934.05
TAGGGAA251.7985818E-434.05
CTGAGAT200.002574697833.99999624
TTCTGAG200.002574697833.99999622
GAGTTTT200.002574697833.99999631
GATGAGT200.002574697833.99999628
TCTGAGA200.002574697833.99999623
GGGATTT200.002574697833.9999967
AGATGAG200.002574697833.99999627
TTAGGGC406.0341335E-833.9999964
TAGGGTG200.002574697833.9999965
TAGGGCA200.002574697833.9999965
Produced by FastQC (version 0.11.5)