FastQCFastQC Report
Fri 17 Jun 2016
SRR1526090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23277
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1320.5670833870344116No Hit
ATTTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1060.4553851441336942No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1030.44249688533745757No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC870.3737595050908622No Hit
ATTTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC710.3050221248442669No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC450.1933238819435494No Hit
ATTTGCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT420.1804356231473128No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC390.16754736435107617No Hit
ATTTGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT290.12458650169695407No Hit
ATTTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC280.12029041543154186No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC260.11169824290071745No Hit
ATTTGCGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT260.11169824290071745No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG240.10310607036989304No Hit
ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG240.10310607036989304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATT150.007782086350.86092838
GTTCAAT354.3597538E-850.86092839
ATAATTA150.007782086350.86092839
TTGTTCA402.224624E-950.6373637
TTTGTTC402.2682798E-950.52631836
TTTTGTC150.007988499550.52631435
TGAGAAC150.007988499550.52631435
AAATATA150.007988499550.52631435
AAGAGAC150.007988499550.52631435
TTTTGTT451.1277734E-1050.52631435
CGCCCAA402.3137545E-950.41575247
CCGCCCA251.9266045E-550.41575246
TCCGCCC309.47397E-750.41575245
GCCCAAT451.1641532E-1050.3056848
CCCAATC451.1641532E-1050.3056849
ACCGACT150.00812835250.30567642
CGACTCC150.00819895850.19607544
CCAATCT451.2187229E-1050.08695650
TGGGGAT150.00827001950.08695615
CCGACTC150.00827001950.08695643