##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526090_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23277 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.06250805516175 39.0 38.0 39.0 33.0 40.0 2 36.37414615285475 39.0 38.0 39.0 31.0 40.0 3 36.41710701550887 39.0 38.0 39.0 32.0 40.0 4 36.35601666881471 39.0 38.0 39.0 32.0 40.0 5 35.18735232203463 38.0 35.0 39.0 26.0 40.0 6 35.98784207586888 39.0 36.0 39.0 30.0 40.0 7 36.283326889203934 39.0 37.0 39.0 32.0 40.0 8 36.241096361214936 39.0 37.0 39.0 31.0 40.0 9 36.35240795635176 39.0 38.0 39.0 32.0 40.0 10 35.17936160158096 39.0 35.0 39.0 28.0 40.0 11 34.24530652575504 38.0 33.0 39.0 26.0 40.0 12 33.485758474030156 38.0 32.0 39.0 21.0 40.0 13 33.1115263994501 38.0 32.0 39.0 18.0 40.0 14 33.853804184388025 38.0 33.0 39.0 22.0 40.0 15 32.733943377583024 38.0 31.0 39.0 15.0 40.0 16 32.10624221334364 36.0 30.0 39.0 17.0 40.0 17 31.15934183958414 36.0 28.0 38.0 13.0 40.0 18 30.41500193323882 35.0 26.0 38.0 12.0 40.0 19 31.765132963869913 36.0 29.0 38.0 16.0 40.0 20 32.13584224771233 36.0 30.0 39.0 18.0 40.0 21 32.23130128452979 37.0 30.0 39.0 17.0 40.0 22 31.37771190445504 36.0 28.0 39.0 13.0 40.0 23 32.12286806719079 36.0 30.0 39.0 16.0 40.0 24 31.53645229196202 36.0 29.0 39.0 14.0 40.0 25 31.947974395325858 36.0 29.0 39.0 16.0 40.0 26 32.40619495639472 37.0 31.0 39.0 16.0 40.0 27 32.503415388581004 37.0 31.0 39.0 17.0 40.0 28 32.467800833440734 37.0 31.0 39.0 17.0 40.0 29 32.06641749366327 36.0 30.0 39.0 15.0 40.0 30 31.842591399235296 36.0 30.0 39.0 13.0 40.0 31 32.52493878077072 37.0 32.0 39.0 14.0 40.0 32 32.385874468359326 37.0 32.0 39.0 14.0 40.0 33 32.13579928684968 36.0 31.0 38.0 11.0 40.0 34 31.91485157021953 36.0 31.0 38.0 10.0 40.0 35 31.830734201142757 36.0 31.0 38.0 4.0 40.0 36 31.015251106242214 35.0 30.0 38.0 4.0 40.0 37 30.690595867165012 35.0 29.0 38.0 4.0 39.0 38 30.111998968939297 35.0 28.0 38.0 4.0 39.0 39 29.172144176655067 35.0 26.0 38.0 4.0 39.0 40 27.074279331528977 33.0 17.0 38.0 4.0 39.0 41 25.481977918116595 31.0 12.0 37.0 4.0 39.0 42 25.98479185462044 31.0 16.0 37.0 4.0 39.0 43 25.889504661253596 31.0 15.0 37.0 4.0 39.0 44 24.99415732267904 30.0 10.0 36.0 4.0 39.0 45 25.850796924002235 31.0 16.0 36.0 4.0 39.0 46 23.843923185977573 28.0 4.0 35.0 4.0 39.0 47 22.914679726768913 27.0 4.0 35.0 4.0 38.0 48 23.593590239292006 28.0 4.0 35.0 4.0 38.0 49 22.52549727198522 27.0 4.0 35.0 4.0 38.0 50 23.187524165485243 28.0 4.0 35.0 4.0 38.0 51 23.423894831808223 29.0 4.0 36.0 4.0 39.0 52 23.177428362761525 29.0 4.0 35.0 4.0 38.0 53 23.05816900803368 29.0 4.0 35.0 4.0 38.0 54 22.552519654594665 28.0 4.0 35.0 4.0 38.0 55 22.12944107917687 28.0 4.0 35.0 4.0 38.0 56 21.847445976715214 27.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.541449982858957 1 2 3.8730544087833536 1 3 3.9480599472100053 1 4 3.4190083286369983 1 5 5.157484010971217 1 6 4.311014287319026 1 7 3.744640868780394 1 8 3.350554842894958 1 9 3.744407757803266 1 10 4.01598337316306 1 11 -1.3363111310482267 1 12 -7.23291288094833 1 13 -17.73308820038574 1 14 -4.591762381145777 1 15 0.22352156608774365 1 16 -1.6002951606453095 1 17 -5.142012509523827 1 18 -5.594025920300101 1 19 0.010532787975716218 1 20 -0.5087223071288847 1 21 5.657516227997117 1 22 7.810655192026964 1 23 6.807190334826789 1 24 7.820441486141554 1 25 7.0642214760923245 1 26 6.3772329519601385 1 27 6.5732945801416385 1 28 4.536310609283941 1 29 6.420927231512131 1 30 6.901487940964785 1 31 3.527614098142312 1 32 4.99759765714283 1 33 4.428569344756681 1 34 3.3819516251497035 1 35 6.062824158254635 1 36 5.057504373614904 1 37 4.19497141725159 1 38 6.327290074289646 1 39 5.602152958474111 1 40 -10.111462263363219 1 41 -1.874438972372765 1 42 -0.9904510356824581 1 43 -9.028289417652559 1 44 -13.820856065177924 1 45 -3.456934255205315 1 46 -5.001274269129624 1 47 -7.73183838983223 1 48 -3.9070184827067003 1 49 -11.807643583125188 1 50 -3.358592317373944 1 51 0.6543919102555371 1 52 0.14633294185366097 1 53 0.20608458934339424 1 54 0.6351184868854958 1 55 0.8731914228357773 1 56 3.9989596123433415 2 1 3.39769998285896 2 2 4.660554408783355 2 3 4.8418099472100025 2 4 4.856508328636998 2 5 5.976234010971218 2 6 5.086014287319024 2 7 4.488390868780392 2 8 4.719304842894957 2 9 4.538157757803269 2 10 5.92223337316306 2 11 4.80743886895177 2 12 2.2920871190516685 2 13 3.9544117996142596 2 14 6.189487618854223 2 15 7.023521566087741 2 16 7.387204839354688 2 17 9.207987490476174 2 18 8.0684740796999 2 19 8.073032787975716 2 20 7.503777692871118 2 21 8.92001622799712 2 22 9.773155192026962 2 23 8.825940334826786 2 24 9.907941486141553 2 25 8.801721476092322 2 26 8.639732951960141 2 27 7.973294580141637 2 28 8.180060609283938 2 29 8.902177231512134 2 30 9.145237940964783 2 31 7.477614098142315 2 32 7.928847657142828 2 33 8.222319344756684 2 34 7.769451625149706 2 35 8.481574158254638 2 36 9.882504373614907 2 37 9.901221417251588 2 38 10.927290074289647 2 39 11.25215295847411 2 40 9.232287736636781 2 41 13.113061027627232 2 42 13.165798964317542 2 43 11.709210582347438 2 44 2.1478939348220756 2 45 12.036815744794684 2 46 12.054975730870375 2 47 9.16191161016777 2 48 11.1742315172933 2 49 4.842356416874811 2 50 12.566407682626053 2 51 14.741891910255536 2 52 14.102582941853658 2 53 13.737334589343394 2 54 14.578868486885497 2 55 14.466941422835777 2 56 14.673959612343339 3 1 2.761724373102858 3 2 4.042261725856527 3 3 4.054005069161228 3 4 4.14004491400285 3 5 5.315258401215118 3 6 4.347599653172686 3 7 4.094488429756005 3 8 3.7613780136266683 3 9 3.850352879754489 3 10 5.065526056089887 3 11 4.583658381146897 3 12 4.70916028978338 3 13 5.780631311809383 3 14 5.625463228610322 3 15 0.9094971758438426 3 16 7.407326790574203 3 17 8.217743588037148 3 18 9.148352128480386 3 19 7.652301080658642 3 20 6.690972814822338 3 21 8.591967447509315 3 22 9.32742348470989 3 23 8.525940334826789 3 24 7.965258559312289 3 25 7.971233671214275 3 26 8.110464659277213 3 27 7.140367750873349 3 28 7.221524023918089 3 29 7.699128451024329 3 30 8.108042819013562 3 31 5.86907751277646 3 32 6.09592082787454 3 33 6.388173003293268 3 34 6.7584760153936045 3 35 7.092549768010734 3 36 5.950797056541731 3 37 7.192684831885739 3 38 8.113875440143307 3 39 8.602152958474111 3 40 9.759726761027029 3 41 12.800865905676012 3 42 11.50726237895169 3 43 11.647015460396219 3 44 12.263747593358662 3 45 11.255718183819074 3 46 13.862292804041111 3 47 12.816179902850696 3 48 12.907768102659151 3 49 14.049063733947985 3 50 13.132261341162643 3 51 11.56445288586529 3 52 12.36050977112195 3 53 11.78611507714827 3 54 12.39655141371476 3 55 12.107185325274802 3 56 10.532496197709197 4 1 2.9713486315076025 4 2 4.283527381756329 4 3 4.163431568831626 4 4 4.5355623826910545 4 5 4.768125902863112 4 6 4.216419692724429 4 7 4.176228706618232 4 8 4.119980518570635 4 9 3.8786982983438065 4 10 5.283719859649544 4 11 6.091898328411233 4 12 6.013708740673291 4 13 6.667249637452098 4 14 5.902325456692061 4 15 6.348521566087744 4 16 7.860853488003336 4 17 9.82420370669239 4 18 9.856987593213415 4 19 8.714924679867611 4 20 7.151750665844091 4 21 8.659205417186303 4 22 8.363020056891827 4 23 7.336751145637603 4 24 8.636319864519933 4 25 7.319289043659889 4 26 6.507300519527703 4 27 6.688159445006502 4 28 6.961141690365022 4 29 7.7393393936742925 4 30 6.701319022045865 4 31 5.960046530574747 4 32 7.562631440926612 4 33 7.076373398810738 4 34 6.616748922447002 4 35 7.261303887984361 4 36 7.114936806047339 4 37 7.364734930765106 4 38 7.604317101316671 4 39 8.142693499014651 4 40 1.6140444933935392 4 41 11.34008805465426 4 42 9.895528694047268 4 43 9.99704842018528 4 44 11.627623664551802 4 45 9.795599528578467 4 46 13.1820027578974 4 47 13.689614312870471 4 48 12.978961247023026 4 49 14.371410470928868 4 50 12.361002277220653 4 51 11.520945964309593 4 52 12.028934293205015 4 53 11.629226481235285 4 54 12.076841459858468 4 55 10.014238720133076 4 56 10.306392044775773 5 1 3.0595420881221145 5 2 4.047396514046511 5 3 4.278652052473163 5 4 4.139403065479101 5 5 5.1130761162343745 5 6 4.3899616557400805 5 7 4.101548763517236 5 8 3.417989053421273 5 9 4.1802630209611635 5 10 4.323549162636745 5 11 5.211386237372828 5 12 4.549981855893773 5 13 5.960990746982677 5 14 5.485540250433168 5 15 7.216942618719326 5 16 7.291152207775745 5 17 8.029040122055118 5 18 9.159263553384111 5 19 7.928295945870456 5 20 7.520882956029013 5 21 7.3384372806287 5 22 8.498155192026964 5 23 8.368045597984686 5 24 7.073730959825767 5 25 7.909616212934427 5 26 6.781838215118036 5 27 6.191715632773221 5 28 5.406376398757626 5 29 6.603493020985816 5 30 5.304448467280572 5 31 6.053929887615997 5 32 4.963058183458617 5 33 5.153898292125099 5 34 5.7931358356760185 5 35 5.189468895096738 5 36 6.787767531509644 5 37 8.430168785672645 5 38 7.761500600605434 5 39 7.944258221632008 5 40 10.620445631373624 5 41 13.655166290785132 5 42 11.50790422747544 5 43 12.896052687610599 5 44 11.661051829558918 5 45 11.511815744794685 5 46 12.707607309817746 5 47 14.53954318911514 5 48 13.407126254135402 5 49 13.422619574769552 5 50 13.187460314205001 5 51 11.572155068150277 5 52 11.23021452080103 5 53 10.974176694606552 5 54 12.814394802674968 5 55 11.953783528098935 5 56 11.821328033395972 6 1 3.0254026855616587 6 2 4.256500354729297 6 3 4.379647785047844 6 4 4.508535355664023 6 5 5.254612389349596 6 6 4.540744017048752 6 7 3.4194719498614745 6 8 3.633494032084144 6 9 3.8786982983438065 6 10 5.553990129919818 6 11 5.848655085167991 6 12 6.094789821754372 6 13 6.045628015830477 6 14 6.253676808043409 6 15 7.132305349871526 6 16 7.590583217733069 6 17 9.3377172202059 6 18 9.0461767824026 6 19 8.498708463651393 6 20 8.15175066584409 6 21 8.199745957726844 6 22 9.795452489324262 6 23 8.14756195644841 6 24 8.284968513168579 6 25 9.021991746362598 6 26 8.047841060068244 6 27 6.931402688249744 6 28 7.069249798473127 6 29 7.766366420701317 6 30 8.349967670694511 6 31 6.311397881926094 6 32 5.697766576061749 6 33 5.8871842096215445 6 34 6.535667841365921 6 35 5.88292550960599 6 36 7.73655842766896 6 37 6.31068087671105 6 38 7.117830614830186 6 39 5.926477282798434 6 40 5.830260709609753 6 41 10.502250216816421 6 42 12.00363680215538 6 43 10.699751122887982 6 44 12.060056096984237 6 45 9.687491420470362 6 46 13.04686762276227 6 47 14.716641339897496 6 48 11.114096382158163 6 49 13.371410470928868 6 50 12.550191466409839 6 51 11.493918937282562 6 52 12.13704240131312 6 53 11.061658913667717 6 54 10.644409027426036 6 55 10.879103584997939 6 56 10.6307163691001 7 1 3.46975880638837 7 2 4.251730879371586 7 3 4.835927594268831 7 4 4.716802446284056 7 5 5.729175187441804 7 6 4.369837816730794 7 7 4.30897910407451 7 8 3.973716607600842 7 9 4.191098934273853 7 10 5.385468667280705 7 11 6.073615339540005 7 12 6.362675354345786 7 13 7.005882387849557 7 14 5.799781736501281 7 15 7.730874507264215 7 16 8.565146015825277 7 17 10.137399255182057 7 18 10.314062314994015 7 19 8.705385729152187 7 20 7.825836516400528 7 21 8.822957404467704 7 22 9.46874342732108 7 23 8.672999158356202 7 24 8.787353250847438 7 25 9.04583912315115 7 26 6.7044388343130805 7 27 7.3821181095534065 7 28 6.262413550460412 7 29 7.783059584453305 7 30 8.267296764494198 7 31 6.26290821578937 7 32 6.602377068907533 7 33 6.13996640358021 7 34 6.5841575075026455 7 35 6.708044746489932 7 36 7.65309260890902 7 37 8.998280240781003 7 38 8.812584191936704 7 39 10.396270605532937 7 40 9.357287736636781 7 41 12.489531615862532 7 42 12.754034258435187 7 43 13.865092935288615 7 44 14.368482170116192 7 45 12.747109862441743 7 46 14.831446319105673 7 47 15.192793963108944 7 48 13.837466811410945 7 49 14.6702975933454 7 50 13.79581944733194 7 51 12.525715439667305 7 52 13.177582941853661 7 53 12.354981648166923 7 54 12.839162604532554 7 55 11.099294364012248 7 56 10.873959612343342 8 1 3.1384894565431694 8 2 3.994764935099141 8 3 4.094441526157375 8 4 4.086771486531738 8 5 4.060444537287005 8 6 4.3899616557400805 8 7 3.838390868780394 8 8 4.181146948158116 8 9 3.864473547276951 8 10 5.270917583689375 8 11 6.053491500530718 8 12 6.3394555401043 8 13 5.27678022066689 8 14 6.35396130306475 8 15 7.795889987140374 8 16 7.81746799724943 8 17 8.79219801679196 8 18 10.132947763910426 8 19 7.849348577449401 8 20 7.731409271818485 8 21 7.864753070102378 8 22 8.129734139395381 8 23 8.262782440089946 8 24 8.652678328246814 8 25 6.541195160302852 8 26 6.281838215118036 8 27 6.586452474878481 8 28 7.248481661915523 8 29 7.971914073617391 8 30 7.14655373043847 8 31 5.738140413931784 8 32 6.805163446616515 8 33 5.627582502651421 8 34 5.714188467254964 8 35 6.636837316149375 8 36 7.445662268351747 8 37 6.7985898383042205 8 38 7.472026916394906 8 39 8.075837169000426 8 40 5.0151824734788875 8 41 11.944639974995653 8 42 11.165798964317542 8 43 11.159210582347441 8 44 10.608420250611548 8 45 10.722342060584158 8 46 13.760238888765116 8 47 13.09217476806251 8 48 12.722915727819615 8 49 13.975251153716915 8 50 13.397986629994474 8 51 12.151102436571325 8 52 12.993372415537873 8 53 12.447860905132867 8 54 11.367026381622338 8 55 11.585362475467356 8 56 11.005538559711763 9 1 3.122699982858954 9 2 3.767697265926209 9 3 4.3703813757814345 9 4 4.195794042922714 9 5 5.504805439542643 9 6 3.9538714301761715 9 7 3.7669622973518244 9 8 4.1014477000378164 9 9 3.9667291863746925 9 10 4.1400905160202015 9 11 4.461010297523202 9 12 4.934944261908814 9 13 6.086554656757119 9 14 5.407344761711364 9 15 6.4913787089448896 9 16 6.983633410783263 9 17 8.265130347619031 9 18 9.314902651128467 9 19 7.251604216547147 9 20 7.350206264299686 9 21 7.955730513711405 9 22 7.269583763455536 9 23 6.468797477683932 9 24 7.857941486141556 9 25 7.637435761806611 9 26 5.882590094817282 9 27 5.280437437284494 9 28 4.651489180712513 9 29 5.0914629457978435 9 30 5.734523655250499 9 31 4.556185526713744 9 32 3.7788476571428298 9 33 5.650890773328108 9 34 5.20516591086399 9 35 6.331574158254632 9 36 7.368218659329187 9 37 7.308364274394446 9 38 8.320147217146786 9 39 7.916438672759828 9 40 7.485859165208211 9 41 10.913061027627236 9 42 11.937227535746114 9 43 12.016353439490295 9 44 13.44075107767922 9 45 11.840387173366114 9 46 14.212118588013233 9 47 14.4154830387392 9 48 14.106374374436154 9 49 15.745927845446243 9 50 14.080693396911773 9 51 12.638320481684108 9 52 12.563297227567947 9 53 12.965906017914822 9 54 13.418154201171212 9 55 13.23122713712149 9 56 11.445388183771914 10 1 2.6084142685732417 10 2 3.6248401230690703 10 3 4.2846670900671455 10 4 3.738651185779858 10 5 4.533376868114075 10 6 2.7253000016047366 10 7 3.6526765830661105 10 8 3.558590557180672 10 9 3.7381577578032648 10 10 3.711519087448771 10 11 4.861010297523201 10 12 5.420658547623102 10 13 5.715126085328546 10 14 5.86448761885422 10 15 6.834235851802031 10 16 7.04077626792612 10 17 8.293701776190456 10 18 8.429188365414188 10 19 7.880175645118573 10 20 7.0644919785854015 10 21 7.927159085139973 10 22 7.898155192026962 10 23 6.783083191969649 10 24 7.372227200427268 10 25 6.7517214760923245 10 26 6.254018666245855 10 27 5.8518660087130705 10 28 6.251489180712515 10 29 6.234320088654989 10 30 6.248809369536211 10 31 7.099042669570881 10 32 5.578847657142827 10 33 6.42231934475668 10 34 6.119451625149701 10 35 7.560145586826067 10 36 8.053932945043478 10 37 6.879792845823022 10 38 7.0915757885753585 10 39 9.345010101331251 10 40 10.68585916520821 10 41 10.741632456198666 10 42 11.508656107174684 10 43 11.616353439490297 10 44 12.383608220536363 10 45 11.268958601937545 10 46 12.469261445156093 10 47 12.24405446731063 10 48 13.106374374436154 10 49 13.460213559731958 10 50 11.452121968340343 10 51 11.752606195969822 10 52 9.991868656139374 10 53 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-0.4412462142816622 115 13 -1.5872548670524083 115 14 -5.227179047812445 115 15 -3.5264784339122563 115 16 1.5455381726880226 115 17 1.9413208238095052 115 18 2.7184740796998987 115 19 2.239699454642384 115 20 1.2621110262044475 115 21 3.928349561330453 115 22 4.664821858693628 115 23 4.317607001493453 115 24 5.382941486141554 115 25 3.543388142758989 115 26 4.023066285293474 115 27 3.1149612468083028 115 28 3.505060609283941 115 29 4.202177231512131 115 30 3.2452379409647847 115 31 2.944280764808976 115 32 3.4038476571428298 115 33 4.022319344756681 115 34 2.6944516251497035 115 35 3.531574158254635 115 36 5.474171040281568 115 37 5.559554750584926 115 38 6.99395674095631 115 39 7.435486291807447 115 40 9.232287736636781 115 41 8.229727694293903 115 42 9.249132297650878 115 43 8.325877249014109 115 44 -1.3521060651779244 115 45 7.886815744794685 115 46 2.529975730870376 115 47 -5.76308838983223 115 48 2.082564850626632 115 49 -2.057643583125188 115 50 1.5997410159593883 115 51 4.300225243588869 115 52 4.427582941853661 115 53 4.154001256010062 115 54 4.687201820218828 115 55 5.4252747561691095 115 56 6.3739596123433415 116 1 2.2512714114303876 116 2 3.5605544087833536 116 3 4.113238518638575 116 4 3.5886511857798524 116 5 4.340519725256932 116 6 4.025300001604741 116 7 3.838390868780394 116 8 3.8728762714663887 116 9 3.9095863292318356 116 10 4.082947658877345 116 11 3.525296011808919 116 12 3.1778014047659546 116 13 3.3651260853285443 116 14 3.528773333139938 116 15 -10.758621291055114 116 16 3.8193476964975446 116 17 6.965130347619027 116 18 4.772045508271329 116 19 4.858747073690001 116 20 4.678777692871115 116 21 5.5771590851399715 116 22 5.819583763455533 116 23 5.240226049112504 116 24 7.043655771855839 116 25 5.930292904663755 116 26 5.975447237674423 116 27 5.3232945801416385 116 28 4.665774894998226 116 29 4.827177231512131 116 30 5.513095083821931 116 31 3.241899812428027 116 32 2.0645619428571145 116 33 3.558033630470966 116 34 2.7123087680068494 116 35 3.6387170153974893 116 36 4.896790087900619 116 37 5.85122141725159 116 38 4.9344329314325 116 39 4.102152958474111 116 40 7.285859165208208 116 41 7.063061027627235 116 42 7.415798964317542 116 43 5.230639153776014 116 44 -0.6021060651779244 116 45 5.7261014590804 116 46 -0.773595697701051 116 47 -7.155945532689373 116 48 -0.3221970541352732 116 49 -4.2897864402680455 116 50 1.4092648254831985 116 51 2.3597490531126795 116 52 3.499011513282234 116 53 2.273048875057679 116 54 2.9610113440283534 116 55 2.1097985656929197 116 56 7.016816755200484 117 1 2.322699982858957 117 2 3.3105544087833536 117 3 3.732286137686195 117 4 3.5767464238750932 117 5 4.638138772875976 117 6 3.525300001604741 117 7 3.195533725923248 117 8 3.587161985752104 117 9 3.623872043517551 117 10 3.60675718268687 117 11 -1.177084940572037 117 12 -7.41743669047214 117 13 -9.78963581943336 117 14 -1.3640838097172043 117 15 -9.651478433912256 117 16 -8.121128493978642 117 17 1.179416061904746 117 18 -5.275573539347722 117 19 -0.17696721202428378 117 20 -0.7259842118907898 117 21 5.136682894663782 117 22 5.974345668217438 117 23 5.049749858636314 117 24 4.734131962332029 117 25 4.370769095139945 117 26 5.749256761483949 117 27 4.8709136277606895 117 28 5.022917752141087 117 29 5.672415326750226 117 30 6.8583331790600255 117 31 4.9561855267137425 117 32 5.58837146666664 117 33 6.403271725709061 117 34 6.2718325775306525 117 35 4.81728844396892 117 36 7.539647230757758 117 37 7.470269036299211 117 38 7.815385312384887 117 39 9.506914863236016 117 40 10.285859165208212 117 41 5.884489599055808 117 42 4.5467513452699215 117 43 -3.555075131938274 117 44 -1.8640108270826872 117 45 4.821339554318495 117 46 -0.5593099834153392 117 47 -4.4892788660227065 117 48 0.34446961253139463 117 49 -1.492167392648998 117 50 0.33783625405462914 117 51 5.752606195969822 117 52 5.1537734180441355 117 53 4.784953636962442 117 54 5.151487534504543 117 55 4.574084279978635 117 56 7.254911993295721 118 1 1.822699982858957 118 2 2.8105544087833536 118 3 3.0418099472100053 118 4 2.9815083286369983 118 5 4.001234010971217 118 6 3.0610142873190256 118 7 2.400890868780394 118 8 2.256804842894958 118 9 2.713157757803266 118 10 2.92223337316306 118 11 0.44493886895177326 118 12 -8.17041288094833 118 13 -3.5455882003857404 118 14 -7.498012381145777 118 15 -8.026478433912256 118 16 -10.97529516064531 118 17 -0.20451250952382694 118 18 -7.094025920300101 118 19 -1.8644672120242838 118 20 -0.6337223071288847 118 21 4.657516227997117 118 22 7.685655192026964 118 23 4.650940334826789 118 24 6.757941486141554 118 25 5.0642214760923245 118 26 5.0022329519601385 118 27 5.6357945801416385 118 28 4.317560609283941 118 29 5.764677231512131 118 30 6.182737940964785 118 31 4.215114098142312 118 32 4.09134765714283 118 33 4.709819344756681 118 34 3.8194516251497035 118 35 5.781574158254635 118 36 6.370004373614904 118 37 6.60122141725159 118 38 8.014790074289646 118 39 5.852152958474111 118 40 9.857287736636781 118 41 7.750561027627235 118 42 8.228298964317542 118 43 -0.21578941765255877 118 44 3.7103939348220756 118 45 3.074315744794685 118 46 -0.9700242691296239 118 47 -0.6380883898322303 118 48 -1.8132684827067003 118 49 -1.6826435831251878 118 50 -1.4835923173739438 118 51 1.5918919102555371 118 52 0.677582941853661 118 53 1.2373345893433942 118 54 2.6663684868854958 118 55 2.9669414228357773 118 56 0.7489596123433415 119 1 2.2226999828589555 119 2 3.1105544087833508 119 3 3.441809947210004 119 4 3.131508328636997 119 5 4.426234010971214 119 6 3.711014287319024 119 7 3.2383908687803924 119 8 1.944304842894958 119 9 3.2381577578032648 119 10 3.7472333731630627 119 11 0.6324388689517733 119 12 -3.657912880948331 119 13 -3.4205882003857404 119 14 -6.885512381145777 119 15 -7.451478433912257 119 16 -10.437795160645308 119 17 0.6079874904761731 119 18 -8.1065259203001 119 19 -0.6269672120242831 119 20 -0.8212223071288847 119 21 4.570016227997119 119 22 5.948155192026967 119 23 4.475940334826792 119 24 3.157941486141553 119 25 4.801721476092322 119 26 5.839732951960137 119 27 4.623294580141636 119 28 4.730060609283942 119 29 6.077177231512131 119 30 6.670237940964782 119 31 5.277614098142312 119 32 6.328847657142827 119 33 6.022319344756681 119 34 5.869451625149701 119 35 6.881574158254637 119 36 5.982504373614901 119 37 5.6512214172515876 119 38 6.127290074289643 119 39 6.152152958474108 119 40 10.807287736636784 119 41 4.713061027627234 119 42 5.715798964317543 119 43 -5.090789417652559 119 44 -2.3521060651779244 119 45 5.361815744794686 119 46 -1.7950242691296232 119 47 -2.0130883898322303 119 48 -6.5007684827067 119 49 -3.7826435831251874 119 50 -2.233592317373944 119 51 5.516891910255538 119 52 6.277582941853662 119 53 6.137334589343393 119 54 5.003868486885494 119 55 7.766941422835778 119 56 11.623959612343342 120 1 1.640881801040777 120 2 2.6741907724197205 120 3 2.905446310846372 120 4 2.7542356013642717 120 5 3.739870374607584 120 6 1.8564688327735723 120 7 2.5202090505985737 120 8 2.6715775701676847 120 9 2.338157757803266 120 10 2.4790515549812397 120 11 -1.1857429492300433 120 12 -7.335185608221057 120 13 -6.0796791094766505 120 14 -6.617330562963961 120 15 0.712157929724107 120 16 -5.69688606973622 120 17 2.7898056722943565 120 18 -4.1110713748455545 120 19 1.7321236970666263 120 20 0.4969595110529319 120 21 4.697288955269844 120 22 6.634518828390597 120 23 6.025940334826789 120 24 5.939759667959734 120 25 5.524448748819598 120 26 5.485187497414685 120 27 5.868749125596185 120 28 4.061878791102121 120 29 4.668086322421225 120 30 5.392965213692058 120 31 3.9367050072332184 120 32 2.7788476571428298 120 33 4.067773890211228 120 34 3.04672435242243 120 35 3.4406650673455417 120 36 6.364322555433084 120 37 5.305766871797044 120 38 7.16819916519874 120 39 6.829425685746838 120 40 7.039105918454965 120 41 -0.050575336009128335 120 42 -1.334201035682458 120 43 -1.6135166903798321 120 44 -14.374833337905198 120 45 0.19363392661286838 120 46 -5.754115178220532 120 47 -0.9221792989231403 120 48 -1.4325866645248837 120 49 -12.319007219488824 120 50 -0.09722868101030713 120 51 4.7396191829828105 120 52 3.4957647600354775 120 53 5.873698225707031 120 54 4.831141214158222 120 55 5.171486877381231 120 56 10.010323248706978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 778.0 5 55.0 6 44.0 7 80.0 8 182.0 9 142.0 10 116.0 11 108.0 12 86.0 13 70.0 14 78.0 15 88.0 16 113.0 17 155.0 18 201.0 19 236.0 20 297.0 21 376.0 22 412.0 23 560.0 24 618.0 25 630.0 26 720.0 27 717.0 28 749.0 29 905.0 30 989.0 31 1181.0 32 1221.0 33 1338.0 34 1442.0 35 1604.0 36 1840.0 37 1883.0 38 1853.0 39 1410.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.21381621342957 0.5971559908922971 0.06014520771577093 0.1288825879623663 8 99.59616789105125 0.30072603857885466 0.05155303518494652 0.05155303518494652 9 98.75413498303047 0.49834600678781626 0.4811616617261674 0.266357348455557 10 65.54968423765949 18.017785797138806 8.794088585298793 7.638441379902909 11 42.18756712634789 22.369721184001374 15.547536194526787 19.895175495123944 12 34.819779181165956 16.604373415818188 20.466554968423768 28.10929243459209 13 25.50586415775229 14.924603686042015 23.224642350818407 36.34488980538729 14 23.19027366069511 21.716716071658716 29.698844352794605 25.39416591485157 15 31.246224216794683 16.9931820143264 26.974195218779666 24.786398550099246 16 33.95942577151207 21.52927017966131 22.788618585059744 21.72268546376687 17 29.87967339922647 20.640309411259132 28.47872797593468 21.001289213579717 18 28.470163680886714 20.359152811788462 23.684323581217512 27.48635992610732 19 30.306416261979457 22.87163178477803 20.134083974386524 26.68786797885599 20 30.482295744955472 23.65013122230349 25.59910510691391 20.26846792582713 21 27.39614211453366 22.133436439403702 24.277183485844393 26.19323796021824 22 24.1740774154745 20.986381406538644 30.411994672853034 24.427546505133822 23 27.53791296129226 20.363448898053875 28.079219830734203 24.01941830991966 24 28.73652102934227 20.079907204536667 24.281479572109806 26.902092194011257 25 26.306066334421725 22.280460560233717 24.61333562467778 26.80013748066678 26 27.601323763269868 21.897967077835563 23.333476597756476 27.167232561138093 27 23.21382512251741 22.719456624537873 23.66520505545525 30.401513197489464 28 26.09984533424987 25.446812167039013 20.957209142464343 27.496133356246776 29 31.95352839931153 24.08777969018933 20.42168674698795 23.53700516351119 30 29.545259176480705 20.587982463680905 27.164102123270006 22.702656236568384 31 33.46368715083799 22.3463687150838 21.908036097980233 22.28190803609798 32 36.345666279919215 22.134845945597526 21.361351037772337 20.158136736710926 33 33.20282247655107 20.74692367266156 23.13053954048705 22.919714310300318 34 25.823395186636244 22.72785981831489 30.753002970680672 20.69574202436819 35 23.99311531841652 26.34251290877797 29.780550774526677 19.88382099827883 36 26.491318549080283 28.700361010830328 24.286573835310296 20.521746604779096 37 30.830464716006883 25.40447504302926 22.956110154905335 20.80895008605852 38 30.27262155992937 27.54209914294328 21.762349799732977 20.422929497394378 39 30.3755000215063 25.312916684588583 20.061077895823477 24.250505398081636 40 23.664353859496963 18.555941023417173 21.569817866435386 36.209887250650475 41 18.72366306094965 18.17628915243929 20.357096311742474 42.74295147486858 42 22.55020253382746 24.15323623200896 23.588727053348272 29.707834180815308 43 20.575297567707434 20.864240124202173 25.94014145247542 32.62032085561498 44 23.49335175271974 22.297530651010188 24.261785529269556 29.94733206700052 45 26.973259268828315 22.22796365671963 26.0862076389786 24.71256943547345 46 23.065315800819143 25.514119422289287 23.388661349428755 28.03190342746282 47 19.327803697943665 21.712458959737344 24.637117677553135 34.32261966476585 48 25.922421948912017 21.346865055474325 28.403715489808206 24.326997505805455 49 24.197232877893192 20.81375802767122 25.029093573552863 29.959915520882717 50 22.893173797021607 21.821468537488165 27.812688301626924 27.472669363863307 51 20.74747823088197 23.73480472454522 28.730925079748253 26.786791964824552 52 22.220309069777453 23.683870690026257 29.146399207954886 24.9494210322414 53 21.81332414030854 23.01559941394467 31.272084805653712 23.89899164009308 54 22.435262182773926 22.043172907061916 30.337369124046702 25.184195786117453 55 21.092100159462138 22.117829591001165 27.87139594018015 28.91867430935655 56 23.502562115144467 22.938466175774018 27.911983809154716 25.6469878999268 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.0 7 1.0 8 4.0 9 7.0 10 11.5 11 16.0 12 22.5 13 30.0 14 31.0 15 29.0 16 27.0 17 26.0 18 25.0 19 28.5 20 32.0 21 34.5 22 44.0 23 51.0 24 48.0 25 45.0 26 56.0 27 67.0 28 79.0 29 103.5 30 116.0 31 140.0 32 164.0 33 209.0 34 254.0 35 274.5 36 295.0 37 310.5 38 356.0 39 386.0 40 459.0 41 532.0 42 658.5 43 785.0 44 1020.5 45 1256.0 46 1243.5 47 1195.0 48 1159.0 49 1205.0 50 1251.0 51 1344.0 52 1437.0 53 1480.0 54 1574.5 55 1626.0 56 1817.0 57 2008.0 58 2049.0 59 2090.0 60 1986.5 61 1883.0 62 1617.0 63 1173.5 64 996.0 65 848.0 66 700.0 67 588.0 68 476.0 69 429.0 70 382.0 71 329.0 72 201.0 73 126.0 74 107.0 75 88.0 76 68.0 77 48.0 78 45.5 79 25.0 80 7.0 81 4.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.44249688533745757 16 0.0472569489195343 17 0.030072603857885464 18 0.0 19 0.03436869012329768 20 0.1460669330240151 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00429608626541221 26 0.0429608626541221 27 0.06444129398118315 28 0.00429608626541221 29 0.15895519182025175 30 0.0472569489195343 31 0.030072603857885464 32 0.02577651759247326 33 0.15036301928942733 34 0.21480431327061048 35 0.15895519182025175 36 0.03866477638870989 37 0.15895519182025175 38 0.24917300339390813 39 0.12029041543154186 40 0.9322507195944494 41 1.1083902564763501 42 0.3050221248442669 43 0.38235167762168665 44 0.4854577479915797 45 0.23198865833225932 46 0.3522790737638012 47 0.5541951282381751 48 0.09880998410448082 49 0.3265025561713279 50 0.18473170941272501 51 0.3393908149675645 52 0.19761996820896163 53 0.3050221248442669 54 0.2921338660480302 55 0.3179103836405035 56 0.23198865833225932 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 23277.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.24032306568715 #Duplication Level Percentage of deduplicated Percentage of total 1 88.9084120360202 69.5622288095545 2 6.9075334943992965 10.808953043777118 3 1.8778827146936086 4.407784508312927 4 0.75225126290358 2.354255273445891 5 0.4282890401932792 1.6754736435107616 6 0.23610806061937184 1.1083902564763501 7 0.1757083241818581 0.9623233234523348 8 0.15923566878980894 0.9966920135756325 9 0.1153085877443444 0.8119603041629077 >10 0.41181638480122995 5.168191777290888 >50 0.010981770261366132 0.678781629935129 >100 0.016472655392049198 1.4649654165055634 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 132 0.5670833870344116 No Hit ATTTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 106 0.4553851441336942 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 103 0.44249688533745757 No Hit ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 87 0.3737595050908622 No Hit ATTTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 71 0.3050221248442669 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 45 0.1933238819435494 No Hit ATTTGCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 42 0.1804356231473128 No Hit ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC 39 0.16754736435107617 No Hit ATTTGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 29 0.12458650169695407 No Hit ATTTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 28 0.12029041543154186 No Hit ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 26 0.11169824290071745 No Hit ATTTGCGGGGATCCCCTCGACCACTCTTTTGGCGCTTCGCTGTCGACAGTGCGCTT 26 0.11169824290071745 No Hit ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 24 0.10310607036989304 No Hit ATTTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 24 0.10310607036989304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.00429608626541221 0.0 0.0 0.0 0.0 11 0.00429608626541221 0.0 0.0 0.0 0.0 12 0.00859217253082442 0.0 0.0 0.0 0.0 13 0.012888258796236628 0.0 0.0 0.0 0.0 14 0.025776517592473257 0.0 0.0 0.0 0.0 15 0.03436869012329768 0.0 0.0 0.0 0.0 16 0.03866477638870989 0.0 0.0 0.0 0.0 17 0.03866477638870989 0.0 0.0 0.0 0.0 18 0.04296086265412209 0.0 0.0 0.0 0.0 19 0.04296086265412209 0.0 0.0 0.0 0.0 20 0.0472569489195343 0.0 0.0 0.0 0.0 21 0.051553035184946513 0.0 0.0 0.0 0.0 22 0.060145207715770935 0.0 0.0 0.0 0.0 23 0.060145207715770935 0.0 0.0 0.0 0.0 24 0.06444129398118315 0.0 0.0 0.0 0.0 25 0.07303346651200757 0.0 0.0 0.0 0.0 26 0.08162563904283197 0.0 0.0 0.0 0.0 27 0.08592172530824418 0.0 0.0 0.0 0.0 28 0.0902178115736564 0.0 0.0 0.0 0.0 29 0.0945138978390686 0.0 0.0 0.0 0.0 30 0.0945138978390686 0.0 0.0 0.0 0.0 31 0.09880998410448082 0.0 0.0 0.0 0.0 32 0.09880998410448082 0.0 0.0 0.0 0.0 33 0.09880998410448082 0.0 0.0 0.0 0.0 34 0.09880998410448082 0.0 0.0 0.0 0.0 35 0.10310607036989303 0.0 0.0 0.0 0.0 36 0.10310607036989303 0.0 0.0 0.0 0.0 37 0.10740215663530524 0.0 0.0 0.0 0.0 38 0.10740215663530524 0.0 0.0 0.0 0.0 39 0.11169824290071745 0.0 0.0 0.0 0.0 40 0.11599432916612966 0.0 0.0 0.0 0.0 41 0.12029041543154187 0.0 0.0 0.0 0.0 42 0.12029041543154187 0.0 0.0 0.0 0.0 43 0.12458650169695408 0.0 0.0 0.0 0.0 44 0.1331786742277785 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAATT 15 0.0077820863 50.860928 38 GTTCAAT 35 4.3597538E-8 50.860928 39 ATAATTA 15 0.0077820863 50.860928 39 TTGTTCA 40 2.224624E-9 50.63736 37 TTTGTTC 40 2.2682798E-9 50.526318 36 TTTTGTC 15 0.0079884995 50.526314 35 TGAGAAC 15 0.0079884995 50.526314 35 AAATATA 15 0.0079884995 50.526314 35 AAGAGAC 15 0.0079884995 50.526314 35 TTTTGTT 45 1.1277734E-10 50.526314 35 CGCCCAA 40 2.3137545E-9 50.415752 47 CCGCCCA 25 1.9266045E-5 50.415752 46 TCCGCCC 30 9.47397E-7 50.415752 45 GCCCAAT 45 1.1641532E-10 50.30568 48 CCCAATC 45 1.1641532E-10 50.30568 49 ACCGACT 15 0.008128352 50.305676 42 CGACTCC 15 0.008198958 50.196075 44 CCAATCT 45 1.2187229E-10 50.086956 50 TGGGGAT 15 0.008270019 50.086956 15 CCGACTC 15 0.008270019 50.086956 43 >>END_MODULE