FastQCFastQC Report
Fri 17 Jun 2016
SRR1526089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGCTCTCTGGAGTGAGTTTTCTTTTTTTCTTTC80.7213706041478809No Hit
ATTTGCGGGGTGAGAAAAATGGTTGTTAAGTTGTAGTTTA30.2705139765554554No Hit
ATTTGCGGGTGGTACCGACTTTTTTTTTTTTTTTTTTTTT30.2705139765554554No Hit
ATTTGCGGGGTTCTTTGTTCCCTCTGCTCAGTGTTCCTAT30.2705139765554554No Hit
ATTTGCGGGATCTTTCTTCCAGTCTAAGTACCAATGCTAT30.2705139765554554No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTA20.18034265103697023No Hit
ATTTGCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC20.18034265103697023No Hit
ATTTGCGGGAGCCTTAGGACCTGAGTTTAAACTTGGAGGG20.18034265103697023No Hit
ATTTGCGGGAATAAAAGTGCACACCTTACCTTACACAAAA20.18034265103697023No Hit
ATTTGCGGGATTTATGACTCTAAGCTTTCCTGATTATTGT20.18034265103697023No Hit
ATTTGCGGGCTGTACCGCAAGTCGATTTTTTTTTTTTTTT20.18034265103697023No Hit
ATTTGCGGGATTTGCAAATAGAGTTGTAGAGCCTTAGAGC20.18034265103697023No Hit
ATTTGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT20.18034265103697023No Hit
ATTTGCGGGGTTACGCCCTTTTGTCGTTTTACTCTTAGAC20.18034265103697023No Hit
ATTTGCGGGATTCCAAGTTGTATATTTGTTTTCCAATAAA20.18034265103697023No Hit
ATTTGCGGGCCTAGCATTGGTGGTTCAGTGGTAGAATTCT20.18034265103697023No Hit
ATTTGCGGGAAGCATGGTACCAGAGTCGATTTTTTTTTTT20.18034265103697023No Hit
ATTTGCGGGAACCTGAGTTTGGAAACTTCAGATAAGATTG20.18034265103697023No Hit
ATTTGCGGGACATGATTAATAATAATGTTCTTTCAGATGT20.18034265103697023No Hit
ATTTGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTA20.18034265103697023No Hit
ATTTGCGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT20.18034265103697023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAA353.1483978E-733.4090925
TTGCGGG1000.033.4090923
TGCGGGG306.062375E-633.4090924
TGCGGGA600.033.4090924
ATTTGCG1050.033.409091
TTTGCGG1100.031.8904952
Produced by FastQC (version 0.11.5)