FastQCFastQC Report
Fri 17 Jun 2016
SRR1526087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC331.8131868131868134No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC100.5494505494505495No Hit
ATTTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT90.49450549450549447No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.49450549450549447No Hit
ATTTGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT70.38461538461538464No Hit
ATTTGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG60.32967032967032966No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC60.32967032967032966No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG50.27472527472527475No Hit
ATTTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.27472527472527475No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAA50.27472527472527475No Hit
ATTTGCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.27472527472527475No Hit
ATTTGCGGGGGTCTTGCTGGTCTGACAGTGACGTCACTCA40.21978021978021978No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGCGTGTAAGAGC40.21978021978021978No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.21978021978021978No Hit
ATTTGCGGGGGTTTTGTTTTCTTTTTTTTAAATTGGCCAT30.16483516483516483No Hit
ATTTGCGGGACCGCAGATGATCTTAAGAATTCTAAACACC30.16483516483516483No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGC30.16483516483516483No Hit
ATTTGCGGGATGATTGACCTGCCTCCTCCTACAGCGGGAG30.16483516483516483No Hit
ATTTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.16483516483516483No Hit
ATTTGCGGGCAGATTTTAGTTTTAGTTTTGATTTCTCTAC30.16483516483516483No Hit
ATTTGCGGGGTTGCTCGACAACCCACACAAAGAACCTATC30.16483516483516483No Hit
ATTTGCGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGA30.16483516483516483No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA30.16483516483516483No Hit
ATTTGCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG30.16483516483516483No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGGGGGTAAGAGC30.16483516483516483No Hit
ATTTGCGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT30.16483516483516483No Hit
ATTTGCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA20.10989010989010989No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCACTGGTTAAAAGC20.10989010989010989No Hit
ATTTGCGGGATAGCAGGATTTGTTCACTGATTCCCATTAT20.10989010989010989No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAG20.10989010989010989No Hit
ATTTGCGGGGTGGGAACGGGGTCACCGGGGTTTGTAGTCC20.10989010989010989No Hit
ATTTGCGGGGGGCTGGTGAGCTGGCTCAGGGGGTAAGAGC20.10989010989010989No Hit
ATTTGCGGGGGACTATCGGGGCGGAGTTAAGGGTTCTTGA20.10989010989010989No Hit
ATTTGCGGGAGGAATGGGCTGGGCTGAAAGTGAAATATTT20.10989010989010989No Hit
ATTTGCGGGGGCTCACAGTTCAGATCTGCATGCATTGCTT20.10989010989010989No Hit
ATTTGCGGGATTGGAGGGCAAGTCTGGTGCCAGCAGCCGC20.10989010989010989No Hit
ATTTGCGGGGCTTGATGTGTCTGTCTCTGTCTTGGTAGTT20.10989010989010989No Hit
ATTTGCGGGGAGACTTTGATTTTCTTCTTTTAGCTCCATA20.10989010989010989No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG20.10989010989010989No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGC20.10989010989010989No Hit
ATTTGCGGGTATGTATTGTGGTTTTTTTGTTTGTTTTATT20.10989010989010989No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAAAG20.10989010989010989No Hit
ATTTGCGGGATTGTGAATGTATTCTGGTTTTGAATAGTTT20.10989010989010989No Hit
ATTTGCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTC20.10989010989010989No Hit
ATTTGCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT20.10989010989010989No Hit
ATTTGCGGGGAGAGGCACTGTGACTGACTTCCCTGGATTT20.10989010989010989No Hit
ATTTGCGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGC20.10989010989010989No Hit
ATTTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.10989010989010989No Hit
ATTTGCGGGGGCTACTTATGTTGTCGTTCTTACTGTGTGT20.10989010989010989No Hit
ATTTGCGGGCAAGGTGCTGCTTCCACAGGGATGTTTATTG20.10989010989010989No Hit
ATTTGCGGGGGGCTGGAGATATGGCTCAGTGGTTAAGAGC20.10989010989010989No Hit
ATTTGCGGGGTGGGGCTGGTGAGATGGCTCAGTGGGTAAG20.10989010989010989No Hit
ATTTGCGGGATTTGAATTGGGTTGGTAACACATTTGCCAA20.10989010989010989No Hit
ATTTGCGGGACTAATCCTAGACCTAGCCCTACACAAATAT20.10989010989010989No Hit
ATTTGCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGT20.10989010989010989No Hit
ATTTGCGGGGGCCGGGGGCATTCGTATTGCGCCGCTAGAG20.10989010989010989No Hit
ATTTGCGGGATTCAATAAGGGCTTGTCTGTCGGTAGGAAA20.10989010989010989No Hit
ATTTGCGGGGGTTAATTGGTTTTAAATCTCTTAGATGCTC20.10989010989010989No Hit
ATTTGCGGGAACCCAGAACTGGGCACTGACGCAGACAAGG20.10989010989010989No Hit
ATTTGCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTAT20.10989010989010989No Hit
ATTTGCGGGGATGTGATTTCCTTGAATGTGGCTTAGGAAA20.10989010989010989No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAG20.10989010989010989No Hit
ATTTGCGGGATGGCGGTTGGAGGAGCAGCAGTGATCACAC20.10989010989010989No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGCGTAAGAGC20.10989010989010989No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC20.10989010989010989No Hit
ATTTGCGGGGGGATGGTGAGATGGCTCAGCGGGTAAGAGC20.10989010989010989No Hit
ATTTGCGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGC20.10989010989010989No Hit
ATTTGCGGGCACATTTCCTGGGCCTTTCAGGAATACCACG20.10989010989010989No Hit
ATTTGCGGGATAGACAGCTAGGTAGAGCTGGAGGCTAGGG20.10989010989010989No Hit
ATTTGCGGGCTTGTCCCTTGCGAGGCATGGAGAGTGACTT20.10989010989010989No Hit
ATTTGCGGGGACTTTGTGTTTTGAAAAGAGCTGATATGAA20.10989010989010989No Hit
ATTTGCGGGGTGTGTGTGTGTGGTGTTTCACCAGGTCTCA20.10989010989010989No Hit
ATTTGCGGGGGGATCCCAACTCTGAATTTCCTGCCACTAA20.10989010989010989No Hit
ATTTGCGGGGAGGTATCCTAATCATATTCTTAATTCTCAT20.10989010989010989No Hit
ATTTGCGGGGCAGCCGGGTTGGATGCCGTGTCTCTGCAAC20.10989010989010989No Hit
ATTTGCGGGGGTAGCTGAGTGAGCTTAAGCCTTTAGTTTG20.10989010989010989No Hit
ATTTGCGGGGAAACAGCTCGCAGGAGAGCTATGCTGGATT20.10989010989010989No Hit
ATTTGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCC20.10989010989010989No Hit
ATTTGCGGGATGGAGAGGCTGACACTTGCTTGTGGTGGGA20.10989010989010989No Hit
ATTTGCGGGGCCTTTGATGCTATACATTTAAAAACCCTTT20.10989010989010989No Hit
ATTTGCGGGGGTGGGCAATGCCAAATGCAGCCATGAAGAA20.10989010989010989No Hit
ATTTGCGGGGTTCATGAATTATGTTTCTTTGTGATTAGTA20.10989010989010989No Hit
ATTTGCGGGGGGGAATTTGGTTTTCAGTCTCTTCCTGTCC20.10989010989010989No Hit
ATTTGCGGGGGACGTGTTCTTCCGGTGGCGGATCGGTGGT20.10989010989010989No Hit
ATTTGCGGGAGCCAGGTCTCTGCAGAACCATATAACCTTG20.10989010989010989No Hit
ATTTGCGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT20.10989010989010989No Hit
ATTTGCGGGCAGCAAGCAGTTCCTGAAATACTACCATTAT20.10989010989010989No Hit
ATTTGCGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGT20.10989010989010989No Hit
ATTTGCGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAG20.10989010989010989No Hit
ATTTGCGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG20.10989010989010989No Hit
ATTTGCGGGACTACTCCTAGCCCTAGCCCTACACAAATAT20.10989010989010989No Hit
ATTTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGC20.10989010989010989No Hit
ATTTGCGGGGAGGCGCGTTGGAGTGTTCGGGTAAAAATGG20.10989010989010989No Hit
ATTTGCGGGGGAGGTAATATTAAATGATCTCCAGCTATAC20.10989010989010989No Hit
ATTTGCGGGATGCCACAAATAGATACATCAACATGATTTA20.10989010989010989No Hit
ATTTGCGGGGGTAAAGACTAATTGACGAGGAAGGCGTGGA20.10989010989010989No Hit
ATTTGCGGGCACCTATAACTTCTCTGTTAACCCAACACCG20.10989010989010989No Hit
ATTTGCGGGGTTCTGAGCCCTCTACCCAGGTTCTCACTGG20.10989010989010989No Hit
ATTTGCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCAT20.10989010989010989No Hit
ATTTGCGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG20.10989010989010989No Hit
ATTTGCGGGGTGTAGAACTGCTCCAGGCTTTCTTCCTTGT20.10989010989010989No Hit
ATTTGCGGGCAACAGAGCTGACAAACTGTACTTCTGCAGC20.10989010989010989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT306.2240833E-635.2058835
GCGGGGG401.9665094E-835.2058835
GCGGGGC306.2240833E-635.2058835
TGCGGGT200.002038685834.24
TGCGGGA200.002038685834.24
TGCGGGG1300.032.8846174
ATTTGCG1850.032.3513531
TTGCGGG1850.032.3513533
TTTGCGG1850.032.3513532
GCGGGGA250.005240689528.1647075
Produced by FastQC (version 0.11.5)