FastQCFastQC Report
Fri 17 Jun 2016
SRR1526086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169840
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG89745.283796514366462No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG24891.4654969382948657No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG11430.6729863400847856No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG9750.5740697126707489No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG7160.4215732454074423No Hit
GGACATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT5520.32501177578897783No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4180.2461139896373057No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4060.2390485162505888No Hit
GGACATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC3460.20372114931700422No Hit
GGACATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC3430.20195478097032502No Hit
GGACATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3100.1825247291568535No Hit
GGACATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA3080.18134715025906736No Hit
GGACATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2980.1754592557701366No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2960.17428167687235044No Hit
GGACATGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT2940.1731040979745643No Hit
GGACATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT2510.14778615167216202No Hit
GGACATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC2440.1436646255299105No Hit
GGACATGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG2310.13601036269430053No Hit
GGACATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC2230.1313000471031559No Hit
GGACATGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT2070.1218794159208667No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1960.11540273198304286No Hit
GGACATGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG1830.10774846914743288No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1730.10186057465850211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT150.00802307350.61227841
CGGATAA150.00802307350.61227841
TCGGATA150.00810890550.4762640
CGCTAAT150.00810890550.4762640
TCCGTGC251.9698353E-550.46119739
CGGCACA150.00811848350.46119338
TTATCAC150.00811848350.46119338
ATGCGCG150.00811848350.46119338
TAGACCT251.9943263E-550.35597237
AAACCGC150.0081857750.35597237
TACTCGG204.030054E-450.35597237
CTAGACC252.011974E-550.28108236
ATAGATT150.00824377550.26613247
CACGATT1400.050.2661347
AACGATT355.038055E-850.2661347
GAATGCT150.00826317850.23625645
CAACGAT402.5502231E-950.23625646
CTCAACG402.570232E-950.1915144
GTAAGAC301.0174681E-650.17661334
ATATGCA355.1095412E-850.1766134
Produced by FastQC (version 0.11.5)