##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526086_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 169840 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.232283325482804 38.0 35.0 39.0 30.0 40.0 2 36.33606924163919 39.0 36.0 39.0 30.0 40.0 3 34.766586198775315 38.0 35.0 39.0 22.0 40.0 4 36.212800282618936 39.0 36.0 39.0 29.0 40.0 5 33.47519430051813 38.0 33.0 39.0 14.0 40.0 6 36.01184055581724 39.0 36.0 39.0 29.0 40.0 7 36.350841968911915 39.0 36.0 39.0 30.0 40.0 8 36.02714319359397 38.0 35.0 39.0 30.0 40.0 9 35.93651083372586 38.0 35.0 39.0 29.0 40.0 10 35.32432289213377 38.0 35.0 39.0 26.0 40.0 11 35.11045690061234 38.0 35.0 39.0 27.0 40.0 12 34.26189354686764 38.0 33.0 39.0 23.0 40.0 13 33.65574069712671 38.0 32.0 39.0 20.0 40.0 14 34.174911681582664 38.0 33.0 39.0 23.0 40.0 15 33.13788271314178 38.0 32.0 39.0 18.0 40.0 16 32.684567828544516 37.0 30.0 39.0 19.0 40.0 17 31.90831370701837 36.0 28.0 39.0 16.0 40.0 18 31.211504945831372 35.0 27.0 38.0 14.0 40.0 19 32.08073480923222 36.0 29.0 38.0 18.0 40.0 20 32.878897786151676 37.0 30.0 39.0 20.0 40.0 21 33.25660621761658 38.0 31.0 39.0 20.0 40.0 22 32.65202543570419 38.0 30.0 39.0 17.0 40.0 23 33.278344324069714 38.0 31.0 39.0 20.0 40.0 24 32.680357983984926 37.0 30.0 39.0 18.0 40.0 25 33.08956076307113 38.0 31.0 39.0 20.0 40.0 26 33.67881535562883 38.0 33.0 39.0 20.0 40.0 27 33.92956311822892 38.0 33.0 39.0 23.0 40.0 28 33.65462199717381 38.0 33.0 39.0 22.0 40.0 29 33.35317357512953 38.0 33.0 39.0 19.0 40.0 30 32.96060409797457 37.0 31.0 39.0 18.0 40.0 31 33.67864460668865 38.0 33.0 39.0 21.0 40.0 32 33.61827013659915 38.0 33.0 39.0 21.0 40.0 33 33.406682760244934 38.0 33.0 39.0 20.0 40.0 34 33.28772373999058 38.0 33.0 39.0 19.0 40.0 35 33.161245878473856 38.0 33.0 39.0 19.0 40.0 36 32.559408855393315 37.0 32.0 38.0 17.0 40.0 37 32.31416038624588 36.0 31.0 38.0 15.0 40.0 38 31.654203956665096 36.0 30.0 38.0 10.0 40.0 39 30.49395902025436 35.0 28.0 38.0 4.0 40.0 40 28.671873528026378 35.0 23.0 38.0 4.0 39.0 41 26.760821950070653 32.0 17.0 38.0 4.0 39.0 42 27.672562411681582 33.0 21.0 38.0 4.0 39.0 43 27.783949599623174 33.0 22.0 38.0 4.0 39.0 44 26.678049929345267 32.0 18.0 38.0 4.0 39.0 45 27.656535562882713 33.0 22.0 38.0 4.0 39.0 46 25.687264484220442 30.0 17.0 36.0 4.0 39.0 47 24.85016486104569 30.0 12.0 36.0 4.0 39.0 48 25.573828308996703 30.0 17.0 36.0 4.0 39.0 49 24.502001884126237 29.0 10.0 36.0 4.0 39.0 50 25.07174399434762 30.0 14.0 36.0 4.0 39.0 51 25.312205605275555 31.0 10.0 36.0 4.0 39.0 52 25.173427932171457 31.0 4.0 36.0 4.0 39.0 53 24.90400965614696 30.0 4.0 36.0 4.0 39.0 54 24.67274493641074 30.0 4.0 36.0 4.0 39.0 55 24.3139366462553 30.0 4.0 36.0 4.0 39.0 56 23.106906500235517 29.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.139662378644701 1 2 3.5674801163492305 1 3 5.530338533004851 1 4 3.920537985344424 1 5 7.594785775343929 1 6 3.8170422973235176 1 7 3.9450560705748927 1 8 4.283399882633674 1 9 4.319637650860898 1 10 4.166968582943198 1 11 0.3426450202411431 1 12 -8.056285421459005 1 13 -18.751758629135644 1 14 -7.9315273576793 1 15 0.20892593399614867 1 16 -1.3624376178797917 1 17 -5.4274716388512125 1 18 -5.002443686280664 1 19 -0.10966813477240578 1 20 -0.6148885437671332 1 21 5.6700826490033265 1 22 6.388498451585317 1 23 5.546811910763235 1 24 6.169083503069523 1 25 5.421782759233661 1 26 5.2690391211478875 1 27 4.69799533493233 1 28 5.2007198944367055 1 29 5.283422771465869 1 30 5.129708578397722 1 31 4.182003223163505 1 32 4.025526108911134 1 33 3.9491003377353273 1 34 3.7842111324692738 1 35 3.7154466043800056 1 36 2.937679191807007 1 37 2.7869973147394695 1 38 3.8905314997761877 1 39 4.403267738665338 1 40 -10.818545918489747 1 41 -1.3244989292759755 1 42 -2.6017118385851106 1 43 -11.08692969784395 1 44 -15.394960877911245 1 45 -3.814497188875169 1 46 -5.770116050823983 1 47 -11.333898940061045 1 48 -5.728856554275936 1 49 -14.253405802996884 1 50 -4.161447347958767 1 51 1.4487118492653508 1 52 1.7274268405892172 1 53 1.755557741513428 1 54 1.462426282150151 1 55 1.3981169801941569 1 56 4.903258559472203 2 1 4.579028383589744 2 2 4.010604928766156 2 3 5.928957921591227 2 4 4.326960829746952 2 5 8.170383540068308 2 6 4.5774361860456665 2 7 4.3978423044784805 2 8 4.610816309908607 2 9 4.817358069903612 2 10 4.800291911845996 2 11 3.7246284700546326 2 12 -0.469882871758422 2 13 1.5518615455131304 2 14 4.301290032879372 2 15 4.938491884631432 2 16 4.455052985101528 2 17 6.8266694807874835 2 18 4.917978214846979 2 19 6.092850123313319 2 20 5.234015971089445 2 21 6.595163756350864 2 22 6.972657150437666 2 23 6.541695359147521 2 24 6.74571029493449 2 25 6.3117197313213005 2 26 5.756212011999548 2 27 5.583173056494736 2 28 5.186828028038729 2 29 5.910486185263412 2 30 5.843781953749666 2 31 4.878840393910551 2 32 4.731810320490549 2 33 5.030507818190959 2 34 4.907394256424169 2 35 4.988338885247252 2 36 5.580549605847516 2 37 5.799545729037231 2 38 6.054847125267479 2 39 6.8099907159838615 2 40 5.318386151426932 2 41 9.292874478947667 2 42 7.868153387443563 2 43 6.695831239142429 2 44 -2.561320265640095 2 45 7.651694981943333 2 46 6.433502694620426 2 47 2.3666112858961625 2 48 6.516552126711357 2 49 -0.5096907291784802 2 50 7.012586867193658 2 51 9.586187016839563 2 52 9.398509748734256 2 53 9.416564758906844 2 54 9.520766404204203 2 55 9.268330932087139 2 56 10.831398164154276 3 1 4.306773680996969 3 2 3.8309913039568073 3 3 5.651250753314663 3 4 3.9260457306112073 3 5 8.004610789687021 3 6 4.3692511326648855 3 7 4.44340661561219 3 8 4.445132527498849 3 9 4.476852223436914 3 10 4.497089064870906 3 11 4.1603300460586965 3 12 4.041276253813514 3 13 4.610834600927465 3 14 4.5932574960212165 3 15 -1.114037347702073 3 16 5.661687453835643 3 17 6.420276496547526 3 18 7.09876113299644 3 19 6.2561953190428525 3 20 5.701161370174351 3 21 6.835415408613194 3 22 7.054749168739651 3 23 6.196525049030594 3 24 6.67723037627664 3 25 6.547256081092524 3 26 5.378276068939048 3 27 5.145069345259358 3 28 4.98230337120091 3 29 5.121073373875507 3 30 5.692129691421247 3 31 4.209140343071709 3 32 4.340084341842857 3 33 4.678245489772042 3 34 4.104305796841047 3 35 4.199281945745469 3 36 5.555206443671608 3 37 5.588259506363105 3 38 6.380749514693001 3 39 6.780161026100789 3 40 8.34192453475179 3 41 10.11198988311645 3 42 9.53857789176486 3 43 9.526868351496269 3 44 9.608481462880494 3 45 9.239074239696262 3 46 10.981303914752608 3 47 11.304956481625698 3 48 10.67881699707231 3 49 11.01752076039956 3 50 10.246254889562746 3 51 9.269308521669249 3 52 10.230195056309242 3 53 10.33323989871366 3 54 10.358654050365868 3 55 9.464683245254399 3 56 9.404377320229518 4 1 4.61398147320476 4 2 4.124238057203556 4 3 5.9233286753925825 4 4 4.3031885877540645 4 5 8.034026374102602 4 6 4.476523859937615 4 7 4.512562459768034 4 8 4.573184475550796 4 9 4.678995080579774 4 10 4.773452701234547 4 11 4.830005370734021 4 12 5.3146528771901345 4 13 5.501224211317076 4 14 5.441309444073163 4 15 5.668495119830396 4 16 7.335583557731741 4 17 7.900341431612457 4 18 8.20785204208735 4 19 7.5161953190428505 4 20 6.767849681862661 4 21 6.830155668353456 4 22 7.663255662246147 4 23 6.658473100978643 4 24 7.109178428224688 4 25 6.8233599771964215 4 26 5.541912432575412 4 27 4.93948492967494 4 28 4.406459215356751 4 29 5.5060733738755125 4 30 5.702779042070599 4 31 4.914854628785996 4 32 4.549629796388313 4 33 5.0094143209408735 4 34 4.546708394243645 4 35 4.960645582109109 4 36 5.0402713787365485 4 37 5.119688077791675 4 38 5.849450813394299 4 39 6.322303883243645 4 40 1.1712751841024414 4 41 10.1187431298697 4 42 8.698383086570054 4 43 8.496348870976789 4 44 9.774195748594781 4 45 8.389139174761194 4 46 11.212797421246115 4 47 10.946320117989334 4 48 10.798752062007381 4 49 10.107455825334622 4 50 11.276449694757549 4 51 10.253983846344578 4 52 10.07571453682872 4 53 10.772980158453915 4 54 10.057680024391846 4 55 10.158774154345306 4 56 9.499052644904843 5 1 4.526172447697888 5 2 3.759254407759478 5 3 5.597036981474993 5 4 4.050814487034643 5 5 8.007616956182396 5 6 4.300905808923879 5 7 4.366453892076734 5 8 4.351196247951059 5 9 4.579858389932291 5 10 4.585578273504005 5 11 4.724590066613679 5 12 4.995490025653183 5 13 5.485449194966524 5 14 5.460472295610117 5 15 6.32653305312013 5 16 6.7844520992621575 5 17 7.803755427688326 5 18 8.640487751701482 5 19 7.478497477316232 5 20 6.555606385590583 5 21 6.9182263027550235 5 22 7.400561090630706 5 23 6.480749098362555 5 24 7.060375288917953 5 25 6.091757622716369 5 26 5.330074630090127 5 27 5.385305727582072 5 28 5.381789496586311 5 29 5.36693668322803 5 30 5.734472959663794 5 31 4.222192758282397 5 32 4.648897291483149 5 33 4.840218768292086 5 34 4.736191716676608 5 35 4.665289139990072 5 36 5.669440770495868 5 37 5.603572969878012 5 38 6.014408301948912 5 39 7.421296688999046 5 40 8.461451770106365 5 41 10.566460592263418 5 42 10.29317706956548 5 43 10.174504528269132 5 44 10.361821647875356 5 45 9.980466839901812 5 46 11.70383731660256 5 47 11.26305000680556 5 48 10.999654612694101 5 49 11.644473483935013 5 50 11.010001035503134 5 51 10.010007391145098 5 52 9.767630821982415 5 53 10.053281008682827 5 54 10.120858572462478 5 55 10.034287561801161 5 56 9.361263240065078 6 1 4.564991949717097 6 2 3.912085302894667 6 3 5.943470615237125 6 4 4.256051041285666 6 5 8.140656992554781 6 6 4.289325482107266 6 7 4.308980168453402 6 8 4.504134120701188 6 9 4.519589876118808 6 10 4.455453377138568 6 11 5.029302430551525 6 12 5.0561195889170705 6 13 5.730855843625289 6 14 5.041106672866675 6 15 6.393888833922993 6 16 7.0658032265387725 6 17 7.8323184508757215 6 18 8.02738566831243 6 19 7.430544761422034 6 20 6.582003112075572 6 21 6.521727145203741 6 22 7.035219163428973 6 23 6.479345017166548 6 24 6.815998299802928 6 25 6.127341051883814 6 26 5.13589688678292 6 27 4.947379488647563 6 28 5.078267258614606 6 29 5.299233225176625 6 30 5.21347860273298 6 31 4.640207788637291 6 32 4.6641549738131225 6 33 4.626785054296736 6 34 4.344653646017896 6 35 4.552534733849562 6 36 5.46845657643847 6 37 5.866751274817698 6 38 6.1702078258985225 6 39 6.864906113726921 6 40 3.4934380769716533 6 41 10.643163542171152 6 42 9.610258720230078 6 43 9.068636806089998 6 44 9.838433666810392 6 45 9.293309502574647 6 46 11.175555109654361 6 47 11.503562429581091 6 48 10.87420998698203 6 49 11.506779921312994 6 50 11.169183726525038 6 51 10.04079019984238 6 52 10.608408014354914 6 53 10.870202192925024 6 54 10.44754146406741 6 55 10.434664657893801 6 56 10.471583905465845 7 1 4.4846746114841025 7 2 3.900121024810666 7 3 6.008804119488715 7 4 4.34180927740924 7 5 7.908592727289644 7 6 4.53591779933155 7 7 4.432938635316624 7 8 4.596145116442472 7 9 4.756696230005062 7 10 4.670269141499261 7 11 4.574930483934999 7 12 4.987937447026432 7 13 5.617950090801575 7 14 5.148101502589363 7 15 6.481925980157804 7 16 7.057171854492456 7 17 7.651187826596786 7 18 8.58611745483552 7 19 7.187612943563927 7 20 7.019511123868927 7 21 6.991367789565572 7 22 7.48880773469477 7 23 7.005474145910721 7 24 7.203612423348339 7 25 6.020545626795862 7 26 5.693218597674683 7 27 5.1640895970382275 7 28 5.30813807289768 7 29 5.846456515637964 7 30 5.398369428695464 7 31 4.310563441046526 7 32 4.59132955257083 7 33 4.2080210781683185 7 34 4.19745029602003 7 35 4.426197654557733 7 36 5.109585206671063 7 37 5.6685003383280765 7 38 5.733212568880191 7 39 7.24276365335859 7 40 7.271700123148065 7 41 10.649373572224277 7 42 9.601399457172633 7 43 9.457547059761183 7 44 10.323314522541136 7 45 10.005426182772343 7 46 11.742647647648063 7 47 11.622293646376658 7 48 11.059399919896613 7 49 11.503276097016961 7 50 11.639180450591756 7 51 10.124426200924862 7 52 10.599420562603711 7 53 10.142161628325045 7 54 9.494600957864503 7 55 9.711139183951715 7 56 8.975778524389344 8 1 4.181886611544677 8 2 3.713487069061266 8 3 5.714633023218674 8 4 3.863820998821261 8 5 7.725093567778494 8 6 4.173598958751839 8 7 4.216910285854986 8 8 4.322433487408503 8 9 4.297335001528388 8 10 4.570606851432174 8 11 4.28945201105023 8 12 4.903585683514251 8 13 5.331896148075973 8 14 5.060281776088971 8 15 5.769206582280987 8 16 6.660642846269294 8 17 7.529748546236963 8 18 8.227614887937154 8 19 6.8700293111377135 8 20 6.1921975079496185 8 21 6.33260626123883 8 22 7.224046176080133 8 23 6.517445432994453 8 24 7.0109966100428736 8 25 6.1836761827300215 8 26 5.420173302140629 8 27 4.979484929674939 8 28 4.900372258835009 8 29 5.614057563598831 8 30 5.235269160647672 8 31 4.817779529971766 8 32 4.446704895202547 8 33 4.189651475091072 8 34 4.372953453532183 8 35 4.612028981318595 8 36 5.158848453835361 8 37 5.398423255657288 8 38 5.648027888493111 8 39 6.9132920255361405 8 40 2.146611152481885 8 41 10.05123324844677 8 42 9.299489805937647 8 43 9.09239630180683 8 44 9.949531716974224 8 45 8.51751862140151 8 46 11.215168962748095 8 47 11.7801541100842 8 48 10.7068943544975 8 49 11.021289817429487 8 50 10.293050485271383 8 51 10.0117704076094 8 52 9.973263943943344 8 53 10.320648142643638 8 54 9.876652356407654 8 55 9.878616051578508 8 56 9.290515095497728 9 1 4.502968060388071 9 2 3.855698563909968 9 3 5.7320470062420625 9 4 4.152860718901607 9 5 7.955606105846272 9 6 4.234005231025542 9 7 4.172622072286664 9 8 4.112931122346623 9 9 4.3926016379568225 9 10 4.590531687821731 9 11 4.265072434817476 9 12 4.913758689410706 9 13 5.5271109475316855 9 14 5.197941336770633 9 15 5.630283495389264 9 16 6.679368952664678 9 17 7.407688674533471 9 18 7.9872857231603795 9 19 7.347998597731376 9 20 6.675107506005727 9 21 6.308307680275959 9 22 7.38033464883333 9 23 6.570529732871339 9 24 6.7479563715927995 9 25 5.8533450740667625 9 26 5.070079347627573 9 27 4.944835153221888 9 28 4.810289319678656 9 29 5.450171734531249 9 30 5.035923602428273 9 31 4.349772661572878 9 32 4.15278925987565 9 33 4.012320431224037 9 34 4.104130152812942 9 35 4.793134404761865 9 36 4.9389300970673915 9 37 5.29165529090643 9 38 5.699719069728133 9 39 6.768861260292827 9 40 2.891573246695586 9 41 8.842200655950172 9 42 9.329620046331605 9 43 8.993144698100487 9 44 9.660589191217728 9 45 8.710092975059258 9 46 11.032767614986803 9 47 11.125858120969962 9 48 10.311896622365055 9 49 11.481747926675904 9 50 11.056852079258295 9 51 9.918723041575571 9 52 10.481541660524698 9 53 10.136869875294455 9 54 10.371241872379922 9 55 10.461814392795379 9 56 10.745595118824365 10 1 4.635548361600918 10 2 3.932501176198386 10 3 6.081273982118375 10 4 4.327091026778454 10 5 8.050168280976216 10 6 4.470877148925048 10 7 4.534144130130187 10 8 4.425039612283982 10 9 4.49639345456351 10 10 4.45318662584652 10 11 5.102170928753239 10 12 5.121009122570776 10 13 5.584121476653369 10 14 5.176416613326673 10 15 6.077571246955301 10 16 7.658377349306026 10 17 8.267111572041134 10 18 8.497948124866358 10 19 7.982211579205455 10 20 7.0593204874798055 10 21 7.125514131028993 10 22 7.568192838890635 10 23 6.7137724357901725 10 24 7.281876137019957 10 25 6.707070250662792 10 26 6.257137857556938 10 27 5.83351301541034 10 28 5.478122186531916 10 29 6.051764430786083 10 30 6.318610527658183 10 31 4.935505035290056 10 32 4.82565344753392 10 33 5.046073596624542 10 34 5.23557757384453 10 35 5.046233165257668 10 36 5.810781356563595 10 37 6.382452305433954 10 38 6.806243126771982 10 39 7.816775427959097 10 40 9.134142885506726 10 41 10.485276758842353 10 42 10.124901933576709 10 43 10.73308205649046 10 44 10.882000626643556 10 45 10.128592242019142 10 46 11.728022846227638 10 47 11.914509327154153 10 48 11.26125021426163 10 49 12.34670194506855 10 50 11.345703205474475 10 51 11.409726640136146 10 52 11.172965091152449 10 53 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3.661885253990178 115 10 3.6566266371252425 115 11 1.456510888337803 115 12 -1.605998725542392 115 13 -1.6542355889982154 115 14 -5.258848201749224 115 15 -6.359103408808597 115 16 1.9268815083360558 115 17 3.0617917731784097 115 18 3.564657086753666 115 19 2.8417444519908344 115 20 3.0113389093981304 115 21 5.041464128679252 115 22 5.81348162125823 115 23 5.484179879749004 115 24 4.960549946879446 115 25 5.38466318266148 115 26 4.445328092060439 115 27 4.512327809338629 115 28 4.118471827022542 115 29 4.963414414337937 115 30 4.757282457730081 115 31 3.741675438034548 115 32 4.097165266698351 115 33 3.6523990818447096 115 34 3.85886814201033 115 35 4.1323744312946005 115 36 5.2315483098978675 115 37 5.318069580681854 115 38 5.779056698838332 115 39 6.636292322550009 115 40 8.120702404281104 115 41 9.449231831919093 115 42 8.282240154357758 115 43 5.959197005501224 115 44 -0.3485788178792113 115 45 7.798219574130609 115 46 3.632976086511487 115 47 -7.092586870975454 115 48 2.9610011424067473 115 49 -0.967058100046355 115 50 3.5566388697444147 115 51 4.57806161828362 115 52 4.636450217333188 115 53 5.267756826872205 115 54 4.29372836911071 115 55 4.405290181670583 115 56 7.050413653838106 116 1 4.531498955722242 116 2 4.095689106154609 116 3 5.9321573467212545 116 4 4.362034741600219 116 5 7.80309980067603 116 6 4.340404978818732 116 7 4.423489033194606 116 8 4.598429230795553 116 9 4.572456619041311 116 10 4.884589064870909 116 11 4.324341035069686 116 12 3.8719081219453813 116 13 4.7653675679604355 116 14 4.987900353164072 116 15 -2.579284600449327 116 16 3.9579786626268465 116 17 5.746512760283789 116 18 5.7795303637656765 116 19 5.9138876267351606 116 20 4.986188842701818 116 21 6.002585738283521 116 22 6.587853564344048 116 23 5.708612961118501 116 24 6.203741365287627 116 25 6.053849487685934 116 26 5.241737607400591 116 27 4.718558356248366 116 28 4.472962711860244 116 29 4.580688758490897 116 30 5.296800021091574 116 31 4.423700782632146 116 32 4.285276649535167 116 33 4.82742131394787 116 34 4.051009093544344 116 35 4.621397330360857 116 36 5.671827322792488 116 37 6.079880385483985 116 38 6.614678086121572 116 39 7.609419267859035 116 40 9.64590805123531 116 41 9.450176696303267 116 42 6.90890756209453 116 43 2.2939013185292367 116 44 4.774003440902472 116 45 7.834541272663291 116 46 2.914545672994368 116 47 -4.8027358260666055 116 48 3.0823884256437424 116 49 -0.11929242641362947 116 50 2.8211175269253843 116 51 6.504473356834087 116 52 6.536239012353196 116 53 7.185025612999372 116 54 6.013187017398838 116 55 6.6742986298697815 116 56 8.950119078471278 117 1 3.7972732141435586 117 2 3.3220277185226337 117 3 5.405919287394958 117 4 3.609332378603739 117 5 7.201132731797195 117 6 3.4901661653446254 117 7 3.5744757099165767 117 8 3.858484534968504 117 9 4.054419917181079 117 10 3.7363047511454184 117 11 0.6366839209419837 117 12 -8.032346528632797 117 13 -9.287391356122018 117 14 -1.4103839605614183 117 15 -8.013453780942037 117 16 -7.115016561103669 117 17 0.8585351333355717 117 18 -2.655487233016629 117 19 0.11436525368337769 117 20 0.1587944233956371 117 21 4.494635763421783 117 22 5.254865882091661 117 23 4.365852780123031 117 24 4.645577715212212 117 25 4.492260749626606 117 26 4.297230317305061 117 27 4.284891940964307 117 28 4.492947628903988 117 29 4.436269452306885 117 30 4.973082072373625 117 31 3.734723909831743 117 32 3.8072412045879567 117 33 3.9646133702575703 117 34 3.857130259959632 117 35 3.8589224686212873 117 36 5.395589263466192 117 37 4.809361280406055 117 38 5.501524491350331 117 39 6.42374179174038 117 40 7.930253199551046 117 41 6.31964627900814 117 42 4.711383680747122 117 43 -5.981488324507467 117 44 -1.8639741860457448 117 45 4.322757713085615 117 46 -3.2057409031745614 117 47 -2.272004302688025 117 48 -1.4862390253366513 117 49 -2.782502582270844 117 50 -0.813152206609054 117 51 4.207590501127701 117 52 4.304401405515591 117 53 4.60373476332617 117 54 4.729218942989586 117 55 4.609944683162894 117 56 7.534489365047445 118 1 3.2683603767439706 118 2 2.4092901043930084 118 3 4.725132977960243 118 4 2.873264923631929 118 5 6.4798834178222435 118 6 3.357800750985497 118 7 3.120751217575112 118 8 3.2541837815882744 118 9 3.4109034775263396 118 10 3.170371507619002 118 11 -0.050438765282635245 118 12 -9.395964749458024 118 13 -7.723952749127058 118 14 -9.71677521935501 118 15 -11.429658939850384 118 16 -10.76901047419053 118 17 -1.803142260268178 118 18 -8.34246024104936 118 19 -1.9109802534762288 118 20 -1.704100352835468 118 21 3.6911133366393685 118 22 4.6433805755008315 118 23 3.739000512498418 118 24 4.255965381729197 118 25 3.949328054920226 118 26 4.4103371376413385 118 27 3.893433576448011 118 28 3.9575487365226074 118 29 4.243783297539633 118 30 4.474354337004673 118 31 3.684930964663856 118 32 3.453252280774155 118 33 4.213578096097013 118 34 3.4973537793928458 118 35 3.733442251088981 118 36 4.890375473115448 118 37 5.028390367868017 118 38 5.809811673907831 118 39 6.948563425228382 118 40 8.69710446932104 118 41 7.490179632298567 118 42 6.7676127881661685 118 43 0.16574512357914628 118 44 0.6957224661520307 118 45 6.03283105539964 118 46 -1.1569458126192558 118 47 -0.8127916099773529 118 48 1.1663578912925985 118 49 -0.9838696430900811 118 50 1.661876481711058 118 51 6.029722916434789 118 52 6.427386986516439 118 53 5.144472177884868 118 54 6.125965936952564 118 55 6.106477138384168 118 56 8.336165760796586 119 1 3.695695428039734 119 2 3.0349039518184995 119 3 5.457665675971626 119 4 3.3857363584547002 119 5 7.121785493963635 119 6 3.2577861645120194 119 7 3.321363849461143 119 8 3.5946320725397953 119 9 3.8596320232549317 119 10 4.021929829202122 119 11 1.4351935583425899 119 12 -4.779151407696951 119 13 -2.259957027825944 119 14 -5.978262067218093 119 15 -9.143131979185238 119 16 -10.988585521596569 119 17 -0.15399556896657884 119 18 -6.440092370188271 119 19 -0.0769257000654271 119 20 -0.5157403586700546 119 21 4.579478192962604 119 22 5.177974828430276 119 23 4.526961809722128 119 24 3.8882403853758163 119 25 4.845618228499259 119 26 5.361715559384908 119 27 4.589791820236606 119 28 4.96328608275687 119 29 5.767729424830922 119 30 5.287307125452184 119 31 4.4139629090407695 119 32 4.497886889613557 119 33 4.186252769116901 119 34 4.682990965175897 119 35 3.9624985062550238 119 36 5.727590429112922 119 37 5.806821835753464 119 38 5.967783181662973 119 39 7.022176494708614 119 40 8.319995508364165 119 41 6.552026279840746 119 42 6.327636126523732 119 43 -4.230229009741219 119 44 -0.6280972450357929 119 45 5.580407269723558 119 46 -2.0591191971673197 119 47 -2.7522091234698394 119 48 -5.312516032954985 119 49 -2.4728568556149995 119 50 -0.04503264455918199 119 51 5.3057962377748 119 52 6.131719169139085 119 53 6.078735804082175 119 54 5.249805096771691 119 55 6.347008086018729 119 56 8.90678860321405 120 1 3.810345109568395 120 2 3.1625597355252353 120 3 5.001650353714261 120 4 3.555216559782039 120 5 7.167173227249457 120 6 3.4077126711264256 120 7 3.5419330891386593 120 8 3.7155620979284194 120 9 3.7743796959643845 120 10 4.018767386549229 120 11 0.46245292318157283 120 12 -6.258773696236435 120 13 -6.149405159312295 120 14 -6.169879367115648 120 15 -0.821854530519257 120 16 -8.675325533177347 120 17 0.14817359944462538 120 18 -6.057882223646914 120 19 -0.3780704152228793 120 20 -0.14012234610936858 120 21 3.8421836403814282 120 22 5.028850067840548 120 23 4.098473100978641 120 24 4.80120640025266 120 25 4.046856480692927 120 26 4.753101243764224 120 27 4.741722691912699 120 28 4.034151523049058 120 29 4.498520926323064 120 30 5.089632188923741 120 31 4.4368126707440325 120 32 3.7227766495351666 120 33 3.6010227125492698 120 34 4.289645457180711 120 35 4.24596026742379 120 36 4.886425224890388 120 37 4.651995770099372 120 38 4.900080184023672 120 39 6.567898288838055 120 40 6.035331128158383 120 41 -4.541956170829604 120 42 -2.1723861441991765 120 43 -6.98057420594629 120 44 -14.616293761894731 120 45 -3.185126559504539 120 46 -7.838950830502137 120 47 -5.6777358260666055 120 48 -4.316212972957658 120 49 -15.177858859980063 120 50 -3.5613125430046857 120 51 2.1862915386522666 120 52 1.6175327186469026 120 53 2.4048682703420283 120 54 1.9471905139023313 120 55 2.3386342942054483 120 56 5.390241456093655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3749.0 5 197.0 6 371.0 7 437.0 8 559.0 9 887.0 10 623.0 11 406.0 12 403.0 13 349.0 14 437.0 15 610.0 16 661.0 17 867.0 18 1096.0 19 1340.0 20 1917.0 21 2535.0 22 3096.0 23 3668.0 24 4303.0 25 4830.0 26 5223.0 27 5575.0 28 6035.0 29 6399.0 30 7050.0 31 7794.0 32 8807.0 33 10018.0 34 10806.0 35 11943.0 36 12757.0 37 14762.0 38 14913.0 39 14412.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.65673575129533 0.23787093735280262 0.07006594441827602 0.03532736693358455 8 99.43652849740933 0.30852567121997176 0.11422515308525671 0.14072067828544513 9 98.51269158075095 0.5964542473077127 0.3880191006671102 0.5028350712742218 10 49.91344795101272 23.335492227979273 19.196891191709845 7.554168629298164 11 34.740343853038155 32.58831841733396 17.36752237399906 15.303815355628828 12 28.651672162034856 15.213141780499292 18.78827131417805 37.346914743287805 13 23.562764955252003 13.050518134715025 21.68805934997645 41.698657560056525 14 22.133184173339615 18.79769194536034 36.04156853509185 23.027555346208196 15 36.99670533121181 15.986537403659035 22.6728813860086 24.343875879120557 16 40.634978614099374 18.55094321837184 21.001190069401076 19.812888098127704 17 26.79473740003062 17.787187430065607 36.20510948045371 19.212965689450066 18 24.064413565708904 18.567475270843147 34.35292039566651 23.01519076778144 19 37.27071380606246 21.486045497920667 19.30421874815923 21.93902194785764 20 25.20456046029051 34.14921712884361 21.01490284851915 19.63131956234673 21 23.808262088132082 20.788878682964743 34.08464636472833 21.31821286417485 22 21.771667451719264 18.725859632595384 24.53838907206783 34.96408384361752 23 22.560645313235987 19.952308054639662 22.300400376825248 35.1866462552991 24 23.29107514028817 19.075069628861975 33.665628367357755 23.968226863492102 25 36.259679081407334 18.719858678050937 21.451494185190636 23.568968055351096 26 24.85050929935255 19.100640379868388 20.259451052473445 35.789399268305615 27 20.550818744807348 19.682283411210825 20.23027381843251 39.536624025549315 28 21.688619863275846 33.575731168043525 20.485070452390904 24.25057851628972 29 38.87316302219706 20.919137593470623 18.94003727030406 21.26766211402826 30 26.064747989513066 19.149851238695614 34.635755736883965 20.14964503490736 31 27.03138802190684 32.605264707614396 21.071197220422825 19.292150050055945 32 41.613074204947 19.200235571260308 20.213780918727913 18.972909305064782 33 27.398770329935925 19.873967660738394 20.444591161230612 32.28267084809507 34 23.299710965610807 32.95110010027724 24.905916356986964 18.843272577124992 35 21.824914792494663 22.9405729246518 37.0608422865095 18.17366999634404 36 23.700286894179055 35.96368756222422 21.242540456792085 19.093485086804634 37 24.753380151303414 22.476753167878393 33.431214731740106 19.338651949078088 38 37.346148853565566 24.402001392478255 19.003788012886325 19.248061741069847 39 25.47472925251882 22.022956792416124 18.883052416240723 33.61926153882433 40 19.9292228766863 17.45083602508075 29.713328899866998 32.906612198365956 41 17.800925650513392 15.511189901129102 26.4964485002796 40.19143594807791 42 31.77408402468188 22.32293112101798 21.322074931357207 24.580909922942933 43 18.600861916287855 17.734222799456877 34.70629907314481 28.958616211110456 44 20.88549582207294 19.40296742222196 21.918002637445817 37.79353411825929 45 22.280177958201065 20.690591109170512 33.89702498259361 23.132205950034813 46 20.260102292727293 23.099729503065237 22.47250735302803 34.167660851179434 47 17.464365381315567 28.974539516114717 23.417964936363582 30.143130166206134 48 22.017640508357736 31.113048909104833 24.001461204433106 22.867849378104324 49 20.346808523200274 29.59621663429236 22.198343301470718 27.85863154103665 50 33.038763988602646 19.756596839180478 24.99395326612118 22.210685906095698 51 20.80153573538098 30.738334317779092 26.584760779681037 21.87536916715889 52 20.46463895818957 20.75658651379231 36.767698214696466 22.011076313321656 53 20.982224557384985 19.208812746840152 37.01200181827842 22.796960877496446 54 21.01076385610424 30.928382588990655 25.453214993862716 22.607638561042396 55 19.52973687939912 31.49239453918466 25.138173744626574 23.839694836789647 56 31.227314309322157 20.360020066692243 25.781568152979016 22.63109747100658 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 4.5 7 8.0 8 8.5 9 9.0 10 38.0 11 67.0 12 106.5 13 183.0 14 220.0 15 215.0 16 210.0 17 218.0 18 226.0 19 256.5 20 287.0 21 269.5 22 292.5 23 333.0 24 359.0 25 385.0 26 449.5 27 514.0 28 575.5 29 691.5 30 746.0 31 864.0 32 982.0 33 1098.0 34 1214.0 35 1459.5 36 1705.0 37 1857.0 38 2183.0 39 2357.0 40 2615.0 41 2873.0 42 3437.5 43 4002.0 44 5263.5 45 6525.0 46 12228.0 47 16017.0 48 14103.0 49 11741.0 50 9379.0 51 9022.0 52 8665.0 53 9087.5 54 10094.0 55 10678.0 56 10520.0 57 10362.0 58 10830.0 59 11298.0 60 11784.0 61 12270.0 62 11313.0 63 9177.0 64 7998.0 65 7259.5 66 6521.0 67 5400.0 68 4279.0 69 3839.0 70 3399.0 71 2954.0 72 2270.5 73 2032.0 74 1500.0 75 968.0 76 758.0 77 548.0 78 515.5 79 542.0 80 601.0 81 396.5 82 192.0 83 118.0 84 44.0 85 24.0 86 4.0 87 2.0 88 1.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0017663683466792276 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.45866698068770606 16 0.06005652378709373 17 0.02237399905793688 18 0.0 19 0.04357041921808761 20 0.12246820536975978 21 0.0023551577955723034 22 0.0 23 0.0 24 0.006476683937823834 25 0.008831841733396137 26 0.05711257654262836 27 0.07595383890720678 28 0.0052991050400376825 29 0.15779557230334432 30 0.06182289213377296 31 0.017663683466792274 32 0.023551577955723033 33 0.11834667922750823 34 0.1825247291568535 35 0.14955252001884126 36 0.0535798398492699 37 0.14543099387658973 38 0.21078662270372114 39 0.12776731040979747 40 0.83843617522374 41 1.0256712199717382 42 0.2855628827131418 43 0.26495525200188413 44 0.4339378238341969 45 0.21314178049929344 46 0.3073480923221856 47 0.4904616109279321 48 0.06888836552048988 49 0.2749646726330664 50 0.1925341497880358 51 0.31794630240226096 52 0.17016015073009894 53 0.26436646255299107 54 0.22609514837494113 55 0.287329251059821 56 0.23845972680169572 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 169840.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.06265512334693 #Duplication Level Percentage of deduplicated Percentage of total 1 87.50986277549852 53.435845705516805 2 7.284988576385631 8.89681490034716 3 2.0901507172024987 3.8289045720105728 4 0.8890013510433731 2.1713913161180387 5 0.5309992360476077 1.6212111610767879 6 0.31399950931873405 1.150418624785801 7 0.21699965977141292 0.9275402770553786 8 0.16099974753730212 0.7864857647052963 9 0.11399982123655358 0.6265018591461786 >10 0.762998803539087 9.12152511883226 >50 0.0749998823924396 3.2784488126262112 >100 0.044999929435463756 5.087732446787408 >500 0.0029999952956975833 1.3696332066903691 >1k 0.001999996863798389 2.21779215635284 >5k 9.999984318991946E-4 5.479754077948896 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 8974 5.283796514366462 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 2489 1.4654969382948657 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 1143 0.6729863400847856 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 975 0.5740697126707489 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 716 0.4215732454074423 No Hit GGACATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 552 0.32501177578897783 No Hit GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 418 0.2461139896373057 No Hit GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 406 0.2390485162505888 No Hit GGACATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 346 0.20372114931700422 No Hit GGACATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 343 0.20195478097032502 No Hit GGACATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 310 0.1825247291568535 No Hit GGACATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 308 0.18134715025906736 No Hit GGACATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 298 0.1754592557701366 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 296 0.17428167687235044 No Hit GGACATGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 294 0.1731040979745643 No Hit GGACATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 251 0.14778615167216202 No Hit GGACATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 244 0.1436646255299105 No Hit GGACATGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 231 0.13601036269430053 No Hit GGACATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 223 0.1313000471031559 No Hit GGACATGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 207 0.1218794159208667 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 196 0.11540273198304286 No Hit GGACATGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 183 0.10774846914743288 No Hit GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 173 0.10186057465850211 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 5.887894488930759E-4 0.0 0.0 0.0 0.0 10 0.002943947244465379 0.0 0.0 0.0 0.0 11 0.010009420631182289 0.0 0.0 0.0 0.0 12 0.011186999528968442 0.0 0.0 0.0 0.0 13 0.012364578426754593 0.0 0.0 0.0 0.0 14 0.022962788506829958 0.0 0.0 0.0 0.0 15 0.023551577955723033 0.0 0.0 0.0 0.0 16 0.02531794630240226 0.0 0.0 0.0 0.0 17 0.027673104097974563 0.0 0.0 0.0 0.0 18 0.028850682995760716 0.0 0.0 0.0 0.0 19 0.03238341968911917 0.0 0.0 0.0 0.0 20 0.034738577484691474 0.0 0.0 0.0 0.0 21 0.0365049458313707 0.0 0.0 0.0 0.0 22 0.03944889307583608 0.0 0.0 0.0 0.0 23 0.040626471973622236 0.0 0.0 0.0 0.0 24 0.04357041921808761 0.0 0.0 0.0 0.0 25 0.044747998115873766 0.0 0.0 0.0 0.0 26 0.04651436646255299 0.0 0.0 0.0 0.0 27 0.04828073480923222 0.0 0.0 0.0 0.0 28 0.0535798398492699 0.0 0.0 0.0 0.0 29 0.05887894488930758 0.0 0.0 0.0 0.0 30 0.059467734338200656 0.0 0.0 0.0 0.0 31 0.06300047103155912 0.0 0.0 0.0 0.0 32 0.06358926048045219 0.0 0.0 0.0 0.0 33 0.0659444182760245 0.0 0.0 0.0 0.0 34 0.06771078662270372 0.0 0.0 0.0 0.0 35 0.07006594441827603 0.0 0.0 0.0 0.0 36 0.07242110221384833 0.0 0.0 0.0 0.0 37 0.07595383890720678 0.0 0.0 0.0 0.0 38 0.07654262835609986 0.0 0.0 5.887894488930759E-4 0.0 39 0.07654262835609986 0.0 0.0 5.887894488930759E-4 0.0 40 0.07889778615167216 0.0 0.0 5.887894488930759E-4 0.0 41 0.08184173339613754 0.0 0.0 5.887894488930759E-4 0.0 42 0.08890720678285445 0.0 0.0 5.887894488930759E-4 0.0 43 0.09126236457842675 0.0 0.0 5.887894488930759E-4 0.0 44 0.09597268016957136 0.0 0.0 5.887894488930759E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGCAT 15 0.008023073 50.612278 41 CGGATAA 15 0.008023073 50.612278 41 TCGGATA 15 0.008108905 50.47626 40 CGCTAAT 15 0.008108905 50.47626 40 TCCGTGC 25 1.9698353E-5 50.461197 39 CGGCACA 15 0.008118483 50.461193 38 TTATCAC 15 0.008118483 50.461193 38 ATGCGCG 15 0.008118483 50.461193 38 TAGACCT 25 1.9943263E-5 50.355972 37 AAACCGC 15 0.00818577 50.355972 37 TACTCGG 20 4.030054E-4 50.355972 37 CTAGACC 25 2.011974E-5 50.281082 36 ATAGATT 15 0.008243775 50.266132 47 CACGATT 140 0.0 50.26613 47 AACGATT 35 5.038055E-8 50.26613 47 GAATGCT 15 0.008263178 50.236256 45 CAACGAT 40 2.5502231E-9 50.236256 46 CTCAACG 40 2.570232E-9 50.19151 44 GTAAGAC 30 1.0174681E-6 50.176613 34 ATATGCA 35 5.1095412E-8 50.17661 34 >>END_MODULE