FastQCFastQC Report
Fri 17 Jun 2016
SRR1526085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7620
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT460.6036745406824147No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT200.26246719160104987No Hit
GGACATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT180.23622047244094488No Hit
GGACATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT160.2099737532808399No Hit
GGACATGGGCTCGTCTTAATCAGCAACATCTTTTGATACA140.1837270341207349No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG140.1837270341207349No Hit
GGACATGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC130.17060367454068243No Hit
GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT130.17060367454068243No Hit
GGACATGGGATGAGATGGACTCAAAGGAATTGACGGGTAC120.15748031496062992No Hit
GGACATGGGCTCGTCTTACTCAGCAACATCTTTTGATACA100.13123359580052493No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC100.13123359580052493No Hit
GGACATGGGATGAGATGGACTTAAATGAATTGACGGGTAC90.11811023622047244No Hit
GGACATGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT80.10498687664041995No Hit
GGACATGGGATGAGATGGACTTAAAGGAATTGACGGGTAC80.10498687664041995No Hit
GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT80.10498687664041995No Hit
GGACATGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC80.10498687664041995No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC80.10498687664041995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC251.7883061E-434.1788116
TGGTTGA251.7883061E-434.1788114
GGTTGAT251.7883061E-434.1788115
TGATCCT251.7883061E-434.1788118
CTGGTTG251.7883061E-434.1788113
GATCCTG251.7883061E-434.1788119
TTGATCC251.7883061E-434.1788117
GTATCAA358.76782E-734.17880618
ATGGGTA454.5620254E-933.953955
ATCCTGC359.243777E-733.9539520
ATGGGGG650.033.953955
ATGGGAG503.1832315E-1033.953955
GTACCTG359.243777E-733.953959
GGGTACC454.5620254E-933.953957
TGGGGGG503.1832315E-1033.953956
GGGGCTG251.860256E-433.9539510
TGGGTAC454.5620254E-933.953956
CATGGGT1150.033.953954
TACCTGG359.243777E-733.9539510
TGGGACT251.860256E-433.953956
Produced by FastQC (version 0.11.5)