##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526085_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7620 Sequences flagged as poor quality 0 Sequence length 40 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18280839895013 33.0 30.0 36.0 16.0 38.0 2 27.956824146981628 31.0 25.0 35.0 4.0 38.0 3 23.96299212598425 26.0 18.0 32.0 4.0 35.0 4 22.896719160104986 23.0 13.0 35.0 4.0 38.0 5 23.550918635170603 26.0 16.0 33.0 4.0 36.0 6 26.906824146981627 31.0 23.0 35.0 4.0 37.0 7 25.64186351706037 30.0 19.0 34.0 4.0 37.0 8 26.693569553805773 32.0 22.0 35.0 4.0 37.0 9 25.52034120734908 30.0 18.0 35.0 4.0 37.0 10 22.446587926509185 26.0 12.0 33.0 4.0 36.0 11 23.926246719160105 28.0 14.0 34.0 4.0 37.0 12 23.06259842519685 27.0 12.0 33.0 4.0 37.0 13 24.007217847769027 29.0 14.0 34.0 4.0 37.0 14 24.208661417322833 29.0 13.0 35.0 4.0 37.0 15 22.406824146981627 27.0 8.0 33.0 4.0 37.0 16 20.05472440944882 23.0 4.0 32.0 4.0 35.0 17 20.804461942257216 25.0 4.0 33.0 4.0 36.0 18 20.277034120734907 24.0 4.0 32.0 4.0 36.0 19 17.34225721784777 17.0 4.0 29.0 4.0 34.0 20 19.136614173228345 22.0 4.0 31.0 4.0 35.0 21 16.958923884514437 15.0 4.0 30.0 4.0 34.0 22 16.356299212598426 13.0 4.0 29.0 4.0 34.0 23 16.49028871391076 15.0 4.0 29.0 4.0 34.0 24 16.013123359580053 12.0 4.0 29.0 4.0 34.0 25 15.670997375328083 9.0 4.0 29.0 4.0 34.0 26 13.610761154855643 4.0 4.0 26.0 4.0 33.0 27 13.301574803149606 4.0 4.0 25.0 4.0 33.0 28 13.234514435695537 4.0 4.0 26.0 4.0 33.0 29 12.58254593175853 4.0 4.0 24.0 4.0 33.0 30 11.843569553805775 4.0 4.0 22.0 4.0 32.0 31 11.48477690288714 4.0 4.0 21.0 4.0 32.0 32 11.033595800524935 4.0 4.0 19.0 4.0 31.0 33 10.447112860892389 4.0 4.0 16.0 4.0 31.0 34 10.141863517060367 4.0 4.0 14.0 4.0 31.0 35 9.675590551181102 4.0 4.0 11.0 4.0 30.0 36 9.788320209973753 4.0 4.0 4.0 4.0 31.0 37 9.625721784776903 4.0 4.0 4.0 4.0 31.0 38 9.409055118110237 4.0 4.0 4.0 4.0 31.0 39 9.016141732283465 4.0 4.0 4.0 4.0 30.0 40 8.929265091863517 4.0 4.0 4.0 4.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.542596771542229 1 2 2.6875085088715025 1 3 -6.223988165921007 1 4 7.117317099867371 1 5 7.2773146197942395 1 6 1.8945444451310571 1 7 3.8171586263515316 1 8 -0.5106426078255808 1 9 -2.651419250769017 1 10 2.2185400457509097 1 11 -2.2425528312620955 1 12 0.9482560419227042 1 13 -8.706592694703076 1 14 -0.1339910895454679 1 15 -1.010191970741662 1 16 1.076292628965195 1 17 2.5100474732942644 1 18 -0.7872045634291638 1 19 0.7454334365325082 1 20 4.115197774923274 1 21 4.063958086900106 1 22 5.820208064209615 1 23 6.806958336428339 1 24 7.09805321302041 1 25 6.323708194782657 1 26 6.919880087513738 1 27 7.749943158802324 1 28 6.5313574850408465 1 29 5.588325156640458 1 30 7.89804289209923 1 31 8.484654500773113 1 32 6.368731166443283 1 33 5.140736198020166 1 34 2.886396524650591 1 35 4.331500893429595 1 36 3.7273897214169427 1 37 5.67974228601202 1 38 4.187754164156933 1 39 5.679660217323059 1 40 2.566642937045719 3 1 6.483773242130461 3 2 8.275743802989151 3 3 15.070129481137815 3 4 16.058493570455607 3 5 7.042020502147182 3 6 10.482779739248706 3 7 11.346570391057416 3 8 7.783475039233242 3 9 -11.357301603710194 3 10 11.924422398692087 3 11 -18.536670478320918 3 12 -17.875273369842002 3 13 -18.824239753526605 3 14 -18.89869697189841 3 15 -17.36313314721225 3 16 -15.217825018093627 3 17 -15.254658409058678 3 18 -15.198969269311515 3 19 -12.254566563467492 3 20 -14.120096342723784 3 21 -11.7595713248646 3 22 -10.768027229908034 3 23 -11.075394604748132 3 24 -10.549005610509003 3 25 -10.499821216982049 3 26 -8.609531677192146 3 27 -8.367703900021205 3 28 -8.29217192672386 3 29 -7.823439549241893 3 30 -7.337251225547828 3 31 -6.750639616873945 3 32 -6.454798245321424 3 33 -6.329852037273952 3 34 -5.643015240055291 3 35 -5.080263812452758 3 36 -5.507904396230115 3 37 -5.32025771398798 3 38 -5.341657600548949 3 39 -5.0262221356181165 3 40 -4.78629823942487 6 1 2.198058956416176 6 2 3.275743802989151 6 3 -2.2155848045764692 6 4 6.344207856169891 6 5 -2.3865509264242455 6 6 -1.5172202607512943 6 7 -5.796286751799727 6 8 -5.930810675052474 6 9 -2.214444460853052 6 10 -2.50414902987934 6 11 0.17761523596479734 6 12 4.839012344443713 6 13 0.46147453218767964 6 14 1.10130302810159 6 15 0.3511525670734663 6 16 -3.6463964466650562 6 17 -0.39751555191582 6 18 3.3724593021170577 6 19 6.4597191508182235 6 20 4.5941893715619315 6 21 3.2404286751353997 6 22 7.374829912949108 6 23 6.781748252394726 6 24 7.450994389490997 6 25 8.785893068732236 6 26 12.104754037093569 6 27 4.775153242835938 6 28 6.422113787561855 6 29 6.319417593615249 6 30 7.377034488737888 6 31 7.96364609741177 6 32 0.25948746896428965 6 33 4.670147962726048 6 34 1.2141276170875663 6 35 2.4911647589758132 6 36 0.7778098894841712 6 37 0.25117085744059153 6 38 0.9440566851653376 6 39 1.9737778643818835 6 40 2.3565589034322727 7 1 3.8171065754637965 7 2 3.4979660252113725 7 3 2.181240592248926 7 4 6.836271348233385 7 5 -3.735757275630597 7 6 0.48277973924870565 7 7 0.3465703910574156 7 8 -2.2165249607667583 7 9 0.9760317296231378 7 10 4.36886684313653 7 11 5.352218410567971 7 12 5.680282185713555 7 13 6.509093579806727 7 14 4.212414139212701 7 15 3.636866852787751 7 16 7.33773053746193 7 17 11.078674924274655 7 18 6.023252952910706 7 19 9.07876676986584 7 20 10.324348101720659 7 21 7.018206452913178 7 22 4.787528325647523 7 23 8.369049839696311 7 24 5.117661056157665 7 25 3.500178783017951 7 26 1.1682461005856322 7 27 4.298962766645463 7 28 5.596716962165029 7 29 1.1765604507581067 7 30 3.5516376633410616 7 31 -0.19508406131838996 7 32 0.6563128657896868 7 33 1.5590368516149375 7 34 -4.309681906721958 7 35 -3.413597145786091 7 36 -2.8412377295634483 7 37 -2.6535910473213127 7 38 -2.7861020449933935 7 39 -3.803999913395894 7 40 -2.2307426838693143 9 1 3.9337732421304636 9 2 3.8257438029891517 9 3 4.870129481137816 9 4 -0.24150642954439405 9 5 3.892020502147183 9 6 8.132779739248704 9 7 5.296570391057415 9 8 5.383475039233243 9 9 5.142698396289806 9 10 9.974422398692088 9 11 5.613329521679081 9 12 6.274726630157996 9 13 8.725760246473396 9 14 6.85130302810159 9 15 6.686866852787752 9 16 4.982174981906372 9 17 3.5953415909413238 9 18 5.3510307306884854 9 19 2.545433436532509 9 20 2.2299036572762176 9 21 6.7404286751354 9 22 1.4319727700919653 9 23 6.474605395251869 9 24 8.100994389490996 9 25 8.550178783017952 9 26 -1.8095316771921457 9 27 -4.517703900021205 9 28 -4.59217192672386 9 29 -5.473439549241894 9 30 -4.487251225547828 9 31 -3.7006396168739455 9 32 -3.5047982453214237 9 33 -2.529852037273952 9 34 -3.8930152400552913 9 35 -1.8802638124527578 9 36 -0.9079043962301157 9 37 -1.020257713987979 9 38 -1.1416576005489496 9 39 -1.3262221356181163 9 40 -2.23629823942487 10 1 5.983773242130461 10 2 8.775743802989151 10 3 -1.9298705188621845 10 4 12.058493570455607 10 5 1.0420205021471816 10 6 7.482779739248706 10 7 2.3465703910574156 10 8 5.283475039233242 10 9 3.6426983962898056 10 10 8.924422398692087 10 11 8.463329521679082 10 12 14.124726630157998 10 13 7.675760246473395 10 14 12.10130302810159 10 15 13.636866852787751 10 16 17.782174981906373 10 17 11.245341590941322 10 18 15.801030730688485 10 19 21.245433436532508 10 20 18.879903657276216 10 21 21.740428675135398 10 22 19.731972770091964 10 23 19.924605395251866 10 24 20.450994389490997 10 25 21.50017878301795 10 26 8.390468322807854 10 27 7.632296099978795 10 28 8.70782807327614 10 29 8.176560450758107 10 30 5.162748774452172 10 31 9.249360383126055 10 32 7.045201754678576 10 33 7.170147962726048 10 34 1.3569847599447087 10 35 6.919736187547242 10 36 6.492095603769885 10 37 5.67974228601202 10 38 6.658342399451051 10 39 7.4737778643818835 10 40 4.21370176057513 11 1 -0.682893424536207 11 2 -0.724256197010849 11 3 -3.7632038521955167 11 4 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0.45378520802953304 12 6 4.071015033366354 12 7 -0.18284137364846842 12 8 3.489357392174419 12 9 0.9368160433486281 12 10 7.21854004575091 12 11 9.345682462855553 12 12 6.360020747805056 12 13 7.469877893532217 12 14 5.630714792807474 12 15 7.872160970434809 12 16 8.958645570141666 12 17 11.568871002706029 12 18 11.565736613041427 12 19 7.921904024767802 12 20 9.468138951393865 12 21 11.711016910429516 12 22 9.879031593621379 12 23 10.042252454075397 12 24 12.392170860079233 12 25 10.794296430076773 12 26 -0.4330610889568529 12 27 -0.3088803706094403 12 28 3.001945720334964 12 29 0.17656045075810667 12 30 -2.1607806373125342 12 31 0.014066265478996698 12 32 -3.9253864806155416 12 33 -2.3886755666857162 12 34 -2.2900740635847034 12 35 -0.5508520477468757 12 36 -2.449080866818351 12 37 -2.732022419870333 12 38 -1.8122458358430666 12 39 -3.085045665029881 12 40 -2.845121768836634 13 1 3.150439908797125 13 2 -2.390922863677517 13 3 1.7367961478044833 13 4 -4.941506429544393 13 5 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4.530398786867753 15 7 0.9179989624859886 15 8 0.45014170589990954 15 9 2.7379364915279005 15 10 4.1625176367873244 15 11 9.796662855012418 15 12 7.839012344443713 15 13 8.413855484568632 15 14 10.9108268376254 15 15 6.446390662311561 15 16 8.02027022000161 15 17 8.554865400465133 15 18 4.420078349736105 15 19 5.935909627008698 15 20 7.641808419180979 15 21 4.097571532278257 15 22 2.565306103425298 15 23 7.067462538109011 15 24 7.3557562942529024 15 25 5.357321640160809 15 26 7.390468322807854 15 27 2.060867528550224 15 28 4.041161406609472 15 29 4.509893784091439 15 30 1.4722725839759825 15 31 4.34459847836415 15 32 4.402344611821434 15 33 3.289195581773667 15 34 4.118889521849471 15 35 2.681640949452005 15 36 -0.22219011051582882 15 37 2.0606946669644017 15 38 2.4678662089748613 15 39 0.49758738819140724 15 40 2.40417795105132 16 1 6.248479124483403 16 2 6.511037920636209 16 3 7.1877765399613445 16 4 11.70555239398502 16 5 2.6890793256765946 16 6 9.541603268660474 16 7 5.346570391057416 16 8 8.0187691568803 16 9 7.995639572760393 16 10 8.336187104574439 16 11 7.8750942275614335 16 12 6.007079571334469 16 13 7.9992896582381015 16 14 9.218950086925119 16 15 7.872160970434809 16 16 6.958645570141666 16 17 7.686518061529558 16 18 7.624560142453191 16 19 8.15719814241486 16 20 9.350491892570332 16 21 7.534546322194222 16 22 5.996678652444908 16 23 9.924605395251868 16 24 3.627464977726291 16 25 4.323708194782657 16 26 -0.7271787360156754 16 27 -1.7206450764917935 16 28 -0.46864251495915354 16 29 -1.7646160198301288 16 30 0.7803958332757013 16 31 1.4846545007731127 16 32 1.6628488135021051 16 33 1.5525009039025193 16 34 0.4158082893564732 16 35 1.037383246370771 16 36 0.6097426625934137 16 37 -0.7908459492820974 16 38 0.6583423994510511 16 39 0.79730727614659 16 40 -0.2568864747189874 17 1 4.220615347393618 17 2 5.381006960883891 17 3 6.5964452706115 17 4 4.584809359929292 17 5 7.042020502147182 17 6 7.377516581353966 17 7 6.030780917373207 17 8 8.5203171444964 17 9 5.221645764710857 17 10 8.345475030271036 17 11 4.937013732205397 17 12 5.38788452489484 17 13 5.965233930683922 17 14 4.364460922838433 17 15 5.110551063314066 17 16 2.3084907713800575 17 17 8.640078433046586 17 18 8.748399151741115 17 19 10.587538699690402 17 20 7.353587867802531 17 21 5.819376043556451 17 22 10.495130664828809 17 23 5.924605395251868 17 24 5.924678600017312 17 25 6.447547204070581 17 26 1.1799420070183793 17 27 6.10598031050511 17 28 4.4446701785392975 17 29 3.1239288718107385 17 30 4.557485616557436 17 31 6.512518277862897 17 32 5.2294122809943655 17 33 0.3017269100944695 17 34 3.2517216020499724 17 35 0.5513151349156633 17 36 1.3868324458751484 17 37 1.7323738649593885 17 38 0.07939503102999801 17 39 2.815883127539779 17 40 2.8452807079435516 18 1 5.102820861178081 18 2 6.704315231560582 18 3 4.260605671614005 18 4 6.487064999027034 18 5 9.137258597385276 18 6 7.76849402496299 18 7 8.537046581533605 18 8 10.688236943995147 18 9 8.92841268200409 18 10 10.543470017739708 18 11 8.272853331202892 18 12 3.934250439681808 18 13 4.842426913140063 18 14 7.434636361434922 18 15 4.874962090882988 18 16 1.6869368866682777 18 17 8.507246352846085 18 18 3.943887873545627 18 19 7.507338198437271 18 20 3.451332228704789 18 21 9.85947629418302 18 22 8.184353722472919 18 23 8.06746253810901 18 24 9.78432772282433 18 25 5.785893068732236 18 26 6.0571349894745214 18 27 8.013248480931175 18 28 9.89830426375233 18 29 7.700369974567632 18 30 5.138939250642647 18 31 3.0112651450308174 18 32 3.87853508801191 18 33 3.622528915107001 18 34 3.595079998039946 18 35 4.634021901832956 18 36 2.301619413293695 18 37 2.298789905059639 18 38 5.420247161355814 18 39 5.25949215009617 18 40 4.40417795105132 19 1 0.3587732421304608 19 2 1.900743802989151 19 3 4.1951294811378155 19 4 -6.691506429544393 19 5 -0.9579794978528184 19 6 5.607779739248706 19 7 0.0965703910574156 19 8 5.033475039233242 19 9 2.0176983962898056 19 10 10.299422398692087 19 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8.058493570455607 115 5 3.0420205021471816 115 6 4.482779739248706 115 7 8.346570391057416 115 8 2.7834750392332417 115 9 8.642698396289806 115 10 -17.075577601307913 115 11 -18.536670478320918 115 12 -17.875273369842002 115 13 -18.824239753526605 115 14 -18.89869697189841 115 15 -17.36313314721225 115 16 -15.217825018093627 115 17 -15.254658409058678 115 18 -15.198969269311515 115 19 -12.254566563467492 115 20 -14.120096342723784 115 21 -11.7595713248646 115 22 -10.768027229908034 115 23 -11.075394604748132 115 24 -10.549005610509003 115 25 -10.499821216982049 115 26 -8.609531677192146 115 27 -8.367703900021205 115 28 -8.29217192672386 115 29 -7.823439549241893 115 30 -7.337251225547828 115 31 -6.750639616873945 115 32 -6.454798245321424 115 33 -6.329852037273952 115 34 -5.643015240055291 115 35 -5.080263812452758 115 36 -5.507904396230115 115 37 -5.32025771398798 115 38 -5.341657600548949 115 39 -5.0262221356181165 115 40 -4.78629823942487 116 1 3.483773242130461 116 2 -0.724256197010849 116 3 3.0701294811378155 116 4 -1.9415064295443933 116 5 -7.957979497852818 116 6 2.4827797392487057 116 7 8.346570391057416 116 8 7.783475039233242 116 9 8.642698396289806 116 10 -13.075577601307913 116 11 10.463329521679082 116 12 6.124726630157998 116 13 7.175760246473395 116 14 10.10130302810159 116 15 1.636866852787751 116 16 -3.2178250180936274 116 17 6.745341590941322 116 18 0.8010307306884847 116 19 8.745433436532508 116 20 -3.1200963427237838 116 21 18.240428675135398 116 22 14.231972770091966 116 23 16.924605395251866 116 24 14.450994389490997 116 25 19.50017878301795 116 26 0.3904683228078536 116 27 11.632296099978795 116 28 -0.29217192672386005 116 29 19.176560450758107 116 30 0.6627487744521723 116 31 4.249360383126055 116 32 3.545201754678576 116 33 19.67014796272605 116 34 20.35698475994471 116 35 -5.080263812452758 116 36 -5.507904396230115 116 37 -5.32025771398798 116 38 -5.341657600548949 116 39 -5.0262221356181165 116 40 -4.78629823942487 117 1 5.483773242130461 117 2 9.275743802989151 117 3 8.070129481137815 117 4 14.058493570455607 117 5 4.042020502147182 117 6 7.482779739248706 117 7 10.346570391057416 117 8 -0.2165249607667583 117 9 11.642698396289806 117 10 7.924422398692087 117 11 7.463329521679082 117 12 5.124726630157998 117 13 4.175760246473395 117 14 2.10130302810159 117 15 11.636866852787751 117 16 -3.2178250180936274 117 17 -0.25465840905867765 117 18 -2.1989692693115153 117 19 7.745433436532508 117 20 3.8799036572762162 117 21 11.2404286751354 117 22 -8.768027229908034 117 23 -3.0753946047481318 117 24 -5.549005610509003 117 25 -4.499821216982049 117 26 12.390468322807854 117 27 16.632296099978795 117 28 3.70782807327614 117 29 -0.8234395492418933 117 30 12.662748774452172 117 31 17.249360383126053 117 32 23.545201754678576 117 33 23.67014796272605 117 34 23.35698475994471 117 35 24.919736187547244 117 36 27.492095603769883 117 37 29.679742286012022 117 38 29.65834239945105 117 39 29.973777864381884 117 40 30.21370176057513 119 1 5.483773242130461 119 2 8.275743802989151 119 3 12.070129481137815 119 4 0.05849357045560666 119 5 13.042020502147182 119 6 4.482779739248706 119 7 3.3465703910574156 119 8 8.783475039233242 119 9 8.642698396289806 119 10 -5.075577601307913 119 11 12.463329521679082 119 12 1.1247266301579977 119 13 12.175760246473395 119 14 8.10130302810159 119 15 13.636866852787751 119 16 -15.217825018093627 119 17 -15.254658409058678 119 18 -15.198969269311515 119 19 -12.254566563467492 119 20 -14.120096342723784 119 21 -11.7595713248646 119 22 -10.768027229908034 119 23 -11.075394604748132 119 24 -10.549005610509003 119 25 -10.499821216982049 119 26 -8.609531677192146 119 27 -8.367703900021205 119 28 -8.29217192672386 119 29 -7.823439549241893 119 30 -7.337251225547828 119 31 -6.750639616873945 119 32 -6.454798245321424 119 33 -6.329852037273952 119 34 -5.643015240055291 119 35 -5.080263812452758 119 36 -5.507904396230115 119 37 -5.32025771398798 119 38 -5.341657600548949 119 39 -5.0262221356181165 119 40 -4.78629823942487 120 1 3.483773242130461 120 2 -22.72425619701085 120 3 -18.929870518862185 120 4 -17.941506429544393 120 5 -17.95797949785282 120 6 -21.517220260751294 120 7 -20.653429608942584 120 8 -21.21652496076676 120 9 -20.357301603710194 120 10 -17.075577601307913 120 11 -18.536670478320918 120 12 -17.875273369842002 120 13 -18.824239753526605 120 14 -18.89869697189841 120 15 -17.36313314721225 120 16 -15.217825018093627 120 17 -15.254658409058678 120 18 -15.198969269311515 120 19 -12.254566563467492 120 20 -14.120096342723784 120 21 -11.7595713248646 120 22 -10.768027229908034 120 23 -11.075394604748132 120 24 -10.549005610509003 120 25 -10.499821216982049 120 26 -8.609531677192146 120 27 -8.367703900021205 120 28 -8.29217192672386 120 29 -7.823439549241893 120 30 -7.337251225547828 120 31 -6.750639616873945 120 32 -6.454798245321424 120 33 -6.329852037273952 120 34 -5.643015240055291 120 35 -5.080263812452758 120 36 -5.507904396230115 120 37 -5.32025771398798 120 38 -5.341657600548949 120 39 -5.0262221356181165 120 40 -4.78629823942487 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 842.0 5 113.0 6 71.0 7 93.0 8 105.0 9 145.0 10 173.0 11 229.0 12 250.0 13 238.0 14 317.0 15 305.0 16 310.0 17 334.0 18 363.0 19 405.0 20 363.0 21 359.0 22 350.0 23 338.0 24 349.0 25 316.0 26 335.0 27 274.0 28 210.0 29 176.0 30 79.0 31 68.0 32 49.0 33 37.0 34 17.0 35 6.0 36 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.63177275118359 0.144660704892162 0.10520778537611783 0.11835875854813256 8 97.9256925298674 0.7745831692267298 0.4332414336352895 0.866482867270579 9 95.67617295308187 2.562754632671836 0.8148245498751477 0.9462478643711395 10 31.377952755905515 40.20997375328084 14.593175853018373 13.818897637795274 11 26.0498687664042 33.976377952755904 24.028871391076116 15.94488188976378 12 38.72703412073491 17.25721784776903 19.619422572178475 24.396325459317588 13 26.824146981627294 21.95538057742782 21.929133858267715 29.291338582677167 14 24.238845144356954 28.25459317585302 28.07086614173228 19.435695538057743 15 29.409448818897637 27.07349081364829 23.62204724409449 19.895013123359583 16 27.979002624671917 21.8503937007874 28.96325459317585 21.20734908136483 17 29.238845144356958 24.383202099737534 24.409448818897637 21.968503937007874 18 28.871391076115486 24.42257217847769 24.12073490813648 22.58530183727034 19 21.128245683405826 18.452616317385 18.00448134967708 42.4146566495321 20 23.097112860892388 30.62992125984252 20.5249343832021 25.74803149606299 21 21.968503937007874 21.968503937007874 31.338582677165356 24.7244094488189 22 20.56700354377215 21.236382727392048 22.79826748917181 35.398346239663994 23 22.772017325108283 26.06641291508072 24.491403071269197 26.6701666885418 24 18.805774278215225 27.755905511811026 26.062992125984252 27.3753280839895 25 25.581089954038084 27.340774786605383 23.007222586999344 24.07091267235719 26 20.66148372644617 24.403742258532084 27.421267624192915 27.51350639082883 27 20.448877805486283 21.131382071137946 27.48392177451109 30.93581834886468 28 16.9424743892829 28.723404255319153 28.329393223010246 26.004728132387704 29 20.33385909568875 24.855415352260778 30.060462670872766 24.750262881177708 30 16.6403785488959 22.20031545741325 36.46161934805468 24.69768664563617 31 16.2900683131897 25.801366263794012 37.073042564372045 20.835522858644246 32 22.48621685481754 20.556576529272775 34.82541349435547 22.131793121554214 33 15.2533473352586 22.564977684431607 37.10947755316356 25.072197427146232 34 12.782152230971128 23.451443569553806 40.09186351706037 23.674540682414698 35 14.846416382252558 23.56261485954319 41.34943554738776 20.24153321081649 36 14.268836965082698 26.187975846678917 37.37201365187713 22.17117353636125 37 13.18623588127134 23.023377987916994 42.855266614131864 20.9351195166798 38 14.50321564509778 21.905761911011943 40.55650347814674 23.034518965743537 39 12.363827273920462 24.8064050400315 37.64273526709542 25.187032418952622 40 10.931758530183727 26.154855643044623 41.06299212598425 21.8503937007874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 5.0 15 9.0 16 24.0 17 39.0 18 39.0 19 45.0 20 51.0 21 57.5 22 64.0 23 64.0 24 88.0 25 112.0 26 130.0 27 148.0 28 148.0 29 153.5 30 159.0 31 187.5 32 216.0 33 216.0 34 265.0 35 314.0 36 345.0 37 376.0 38 376.0 39 398.0 40 420.0 41 450.0 42 480.0 43 480.0 44 486.5 45 493.0 46 517.0 47 541.0 48 541.0 49 526.5 50 512.0 51 516.0 52 520.0 53 520.0 54 521.0 55 522.0 56 517.5 57 513.0 58 513.0 59 528.0 60 543.0 61 496.0 62 449.0 63 449.0 64 407.0 65 365.0 66 330.0 67 295.0 68 295.0 69 250.0 70 205.0 71 170.5 72 136.0 73 136.0 74 108.5 75 81.0 76 55.0 77 29.0 78 29.0 79 22.5 80 16.0 81 11.5 82 7.0 83 7.0 84 5.0 85 3.0 86 2.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.2099737532808399 8 0.03937007874015748 9 0.1443569553805774 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.4330708661417323 20 0.0 21 0.0 22 0.013123359580052493 23 0.013123359580052493 24 0.0 25 0.06561679790026247 26 0.4068241469816273 27 0.013123359580052493 28 0.07874015748031496 29 0.15748031496062992 30 0.15748031496062992 31 0.10498687664041995 32 0.026246719160104987 33 0.026246719160104987 34 0.0 35 0.026246719160104987 36 0.026246719160104987 37 0.07874015748031496 38 0.013123359580052493 39 0.013123359580052493 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 7620.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.96850393700787 #Duplication Level Percentage of deduplicated Percentage of total 1 96.27568493150685 88.54330708661418 2 2.3401826484018264 4.304461942257218 3 0.6135844748858447 1.6929133858267715 4 0.28538812785388123 1.0498687664041995 5 0.08561643835616438 0.39370078740157477 6 0.08561643835616438 0.47244094488188976 7 0.07134703196347031 0.45931758530183725 8 0.07134703196347031 0.5249343832020997 9 0.014269406392694063 0.11811023622047244 >10 0.1569634703196347 2.440944881889764 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 46 0.6036745406824147 No Hit GGACATGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT 20 0.26246719160104987 No Hit GGACATGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT 18 0.23622047244094488 No Hit GGACATGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT 16 0.2099737532808399 No Hit GGACATGGGCTCGTCTTAATCAGCAACATCTTTTGATACA 14 0.1837270341207349 No Hit GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 14 0.1837270341207349 No Hit GGACATGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC 13 0.17060367454068243 No Hit GGACATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 13 0.17060367454068243 No Hit GGACATGGGATGAGATGGACTCAAAGGAATTGACGGGTAC 12 0.15748031496062992 No Hit GGACATGGGCTCGTCTTACTCAGCAACATCTTTTGATACA 10 0.13123359580052493 No Hit GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC 10 0.13123359580052493 No Hit GGACATGGGATGAGATGGACTTAAATGAATTGACGGGTAC 9 0.11811023622047244 No Hit GGACATGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT 8 0.10498687664041995 No Hit GGACATGGGATGAGATGGACTTAAAGGAATTGACGGGTAC 8 0.10498687664041995 No Hit GGACATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 8 0.10498687664041995 No Hit GGACATGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC 8 0.10498687664041995 No Hit GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 8 0.10498687664041995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.013123359580052493 0.0 0.0 0.0 0.0 12 0.026246719160104987 0.0 0.0 0.0 0.0 13 0.026246719160104987 0.0 0.0 0.0 0.0 14 0.026246719160104987 0.0 0.0 0.0 0.0 15 0.03937007874015748 0.0 0.0 0.0 0.0 16 0.05249343832020997 0.0 0.0 0.0 0.0 17 0.05249343832020997 0.0 0.0 0.0 0.0 18 0.05249343832020997 0.0 0.0 0.0 0.0 19 0.05249343832020997 0.0 0.0 0.0 0.0 20 0.06561679790026247 0.0 0.0 0.0 0.0 21 0.06561679790026247 0.0 0.0 0.0 0.0 22 0.06561679790026247 0.0 0.0 0.0 0.0 23 0.06561679790026247 0.0 0.0 0.0 0.0 24 0.06561679790026247 0.0 0.0 0.0 0.0 25 0.06561679790026247 0.0 0.0 0.0 0.0 26 0.06561679790026247 0.0 0.0 0.0 0.0 27 0.06561679790026247 0.0 0.0 0.0 0.0 28 0.07874015748031496 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGATC 25 1.7883061E-4 34.17881 16 TGGTTGA 25 1.7883061E-4 34.17881 14 GGTTGAT 25 1.7883061E-4 34.17881 15 TGATCCT 25 1.7883061E-4 34.17881 18 CTGGTTG 25 1.7883061E-4 34.17881 13 GATCCTG 25 1.7883061E-4 34.17881 19 TTGATCC 25 1.7883061E-4 34.17881 17 GTATCAA 35 8.76782E-7 34.178806 18 ATGGGTA 45 4.5620254E-9 33.95395 5 ATCCTGC 35 9.243777E-7 33.95395 20 ATGGGGG 65 0.0 33.95395 5 ATGGGAG 50 3.1832315E-10 33.95395 5 GTACCTG 35 9.243777E-7 33.95395 9 GGGTACC 45 4.5620254E-9 33.95395 7 TGGGGGG 50 3.1832315E-10 33.95395 6 GGGGCTG 25 1.860256E-4 33.95395 10 TGGGTAC 45 4.5620254E-9 33.95395 6 CATGGGT 115 0.0 33.95395 4 TACCTGG 35 9.243777E-7 33.95395 10 TGGGACT 25 1.860256E-4 33.95395 6 >>END_MODULE