FastQCFastQC Report
Fri 17 Jun 2016
SRR1526083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7104
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT4225.940315315315315No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC500.7038288288288288No Hit
GGACATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT400.5630630630630631No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG360.5067567567567568No Hit
GGACATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT300.4222972972972973No Hit
GGACATGGGTACCTGGTTGATACTGCCAGTAGCATATGCT210.2956081081081081No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC200.28153153153153154No Hit
GGACATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG170.23930180180180177No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA160.22522522522522523No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGAAGCATATGCT150.21114864864864866No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA140.19707207207207206No Hit
GGACATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA130.18299549549549549No Hit
GGACATGGGTACATGGTTGATCCTGCCAGTAGCATATGCT120.16891891891891891No Hit
GGACATGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT110.15484234234234234No Hit
GGACATGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC110.15484234234234234No Hit
GGACATGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAG110.15484234234234234No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.15484234234234234No Hit
GGACATGGGTACCTGGTTAATCCTGCCAGTAGCATATGCT100.14076576576576577No Hit
GGACATGGGTACCTGGTTGACCCTGCCAGTAGCATATGCT100.14076576576576577No Hit
GGACATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG100.14076576576576577No Hit
GGACATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC90.1266891891891892No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGAT80.11261261261261261No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC80.11261261261261261No Hit
GGACATGGGATTCGTAGGAGTAAACATAACATTCTTCCCT80.11261261261261261No Hit
GGACATGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT80.11261261261261261No Hit
GGACATGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCA80.11261261261261261No Hit
GGACATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT80.11261261261261261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG551.4551915E-1134.9051126
CAGTAGC551.4551915E-1134.9051127
AGTAGCA601.8189894E-1234.65217628
GCCAGTA551.6370905E-1134.65217625
TGCCAGT601.8189894E-1234.65217624
GTAGCAT601.8189894E-1234.65217629
CTGCCAG700.034.65217223
CCTGCCA750.034.40287822
GTTGATC650.033.91489416
TGGTTGA700.033.91489414
GGTTGAT650.033.91489415
TGATCCT650.033.91489418
TAGCATA552.1827873E-1133.91489430
CTGGTTG601.8189894E-1233.91489413
GATCCTG650.033.91489419
TTGATCC650.033.91489417
AGCATAT650.033.91489431
GCATATG650.033.91489432
ATGGGTC301.3748107E-533.6760565
ATGGGGT552.3646862E-1133.6760565
Produced by FastQC (version 0.11.5)