FastQCFastQC Report
Fri 17 Jun 2016
SRR1526082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71993
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC12941.7973969691497786No Hit
CGCACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT12491.7348908921700723No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG10321.4334726987345991No Hit
CGCACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC4370.6070034586695929No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3210.44587668245523865No Hit
CGCACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT3130.4347644909921798No Hit
CGCACCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC2020.28058283444223747No Hit
CGCACCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1670.23196699679135468No Hit
CGCACCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1590.22085480532829582No Hit
CGCACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC1490.2069645659994722No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1370.1902962788048838No Hit
CGCACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1360.18890725487200144No Hit
CGCACCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1330.18474018307335435No Hit
CGCACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC1300.18057311127470727No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1250.17362799161029543No Hit
CGCACCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1120.1555706804828247No Hit
CGCACCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1060.14723653688553054No Hit
CGCACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT1010.14029141722111874No Hit
CGCACCGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG920.12779020182517745No Hit
CGCACCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC890.12362313002653035No Hit
CGCACCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT880.12223410609364799No Hit
CGCACCGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG870.12084508216076563No Hit
CGCACCGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT860.11945605822788327No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT820.11389996249635381No Hit
CGCACCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG810.11251093856347145No Hit
CGCACCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC810.11251093856347145No Hit
CGCACCGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG790.10973289069770673No Hit
CGCACCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC780.10834386676482435No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG770.10695484283194198No Hit
CGCACCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG740.10278777103329491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGAA950.050.50951441
CAATTCT150.00806873550.50951441
TATTGAA203.9543514E-450.50951441
GCGAATT150.00813644350.40295438
CGCATTG950.050.40295439
GCGCATT900.050.40295438
GCATTGA950.050.40295440
TCAATCA150.00813644350.40295440
CGAATTG150.00813644350.40295439
TGCTCGT600.050.40295437
GGCGCAT900.050.40295437
TATAATT203.9958322E-450.40295438
ATGCTCG600.050.29684436
TATGCCT150.00820457350.29684435
GACGCAA204.0376582E-450.29684436
ATGCCTG150.00820457350.29684436
TCCACGA150.00827312650.19117745
ATTATAC150.00827312650.19117742
CCACGAT150.00827312650.19117746
ATTGACC301.0071435E-650.19117742
Produced by FastQC (version 0.11.5)