FastQCFastQC Report
Fri 17 Jun 2016
SRR1526081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGATGAGATGGACTTAAATGAATTGACGGGTA150.33400133600534404No Hit
CGCACCGGGGATGAGATGGACTCAAAGGAATTGACGGGTA120.26720106880427524No Hit
CGCACCGGGGATGAGATGGACTTAAAGGAATTGACGGGTA100.222667557336896No Hit
CGCACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15586729013582723No Hit
CGCACCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.15586729013582723No Hit
CGCACCGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA60.13360053440213762No Hit
CGCACCGGGGATGAGATGGACTCAAATGAATTGACGGGTA60.13360053440213762No Hit
CGCACCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT60.13360053440213762No Hit
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.13360053440213762No Hit
CGCACCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT50.111333778668448No Hit
CGCACCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA50.111333778668448No Hit
CGCACCGGGGGAAAAAAACAAAAAAAAAAAAAAAAAAAAA50.111333778668448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC601.8189894E-1234.0000044
CGGGGAT301.1799473E-534.0000046
CGGGAAG601.8189894E-1234.0000046
CCGGGAG301.1799473E-534.0000045
ACCGGGT502.419256E-1034.04
ACCGGGG1000.034.04
ACCGGGA1950.034.04
CACCGGG4050.034.03
CGGGATG358.033021E-734.06
CGGGATC251.7228849E-434.06
GGAAGCA251.7228849E-434.08
CCGGGGG251.7228849E-434.05
CCGGGGA502.419256E-1034.05
CCGGGCA358.033021E-734.05
GCACCGG4150.034.02
CCGGGAT700.034.05
CCGGGAA750.034.05
GGGAAGC358.033021E-734.07
CGCACCG4300.034.01
CGGGCAG200.002502351533.9999966
Produced by FastQC (version 0.11.5)