FastQCFastQC Report
Fri 17 Jun 2016
SRR1526080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492
Sequences flagged as poor quality0
Sequence length40
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.2407704654895666No Hit
CGCACCGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT30.1203852327447833No Hit
CGCACCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1203852327447833No Hit
CGCACCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1203852327447833No Hit
CGCACCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.1203852327447833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGT200.0022826834.0000044
CCGGGGA200.0022826834.0000045
GCACCGG2400.034.0000042
CCGGGAT309.688099E-634.0000045
CGCACCG2400.034.0000041
ACCGGGG1050.034.04
ACCGGGC501.4188117E-1034.04
ACCGGGA559.094947E-1234.04
CGGGGGG251.4988864E-434.06
CACCGGG2300.034.03
CCGGGGT251.4988864E-434.05
CCGGGGC251.4988864E-434.05
CCGGGGG356.15737E-733.9999965
Produced by FastQC (version 0.11.5)