FastQCFastQC Report
Fri 17 Jun 2016
SRR1526079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3222
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT762.3587833643699563No Hit
CGCACCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG742.2967101179391682No Hit
CGCACCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT411.2725015518311609No Hit
CGCACCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT140.4345127250155183No Hit
CGCACCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT130.40347610180012417No Hit
CGCACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.3414028553693358No Hit
CGCACCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT110.3414028553693358No Hit
CGCACCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG100.31036623215394166No Hit
CGCACCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG80.2482929857231533No Hit
CGCACCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG70.21725636250775915No Hit
CGCACCGGGTCACAAATCCTCTTCTGAGATGAGTTTTTGT60.186219739292365No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT60.186219739292365No Hit
CGCACCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT60.186219739292365No Hit
CGCACCGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT50.15518311607697083No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC50.15518311607697083No Hit
CGCACCGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG50.15518311607697083No Hit
CGCACCGGGTCAACGATCCTCTTCTGAAGGAGATTGGGCG40.12414649286157665No Hit
CGCACCGGGGAACTTTCTGTTTTGTCTCTTGCCCGTCCGG40.12414649286157665No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA40.12414649286157665No Hit
CGCACCGGGCGCTGAGCTTAGAATAGCCCATGTGTTACCT40.12414649286157665No Hit
CGCACCGGGGTCGATTCCATTGTGATAAGCGCACAAACAG40.12414649286157665No Hit
CGCACCGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT40.12414649286157665No Hit
CGCACCGGGATTTCCTGGGCCTTTCAGGAATACCACGACG40.12414649286157665No Hit
CGCACCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTAC40.12414649286157665No Hit
CGCACCGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAA40.12414649286157665No Hit
CGCACCGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT40.12414649286157665No Hit
CGCACCGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA40.12414649286157665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGAA200.002247059534.4285722
CTGAAGG251.492586E-434.4285724
TGAAGGA251.492586E-434.4285725
TCTGAAG200.002247059534.4285723
AAGGAGA251.492586E-434.4285727
CTTCTGA356.376904E-734.4285721
GAAGGAG251.492586E-434.4285726
GGAGATT251.6437449E-433.89062529
ACCGGGT501.9463187E-1033.8906254
ACCGGGG800.033.8906254
ACCGGGC501.9463187E-1033.8906254
ACCGGGA1050.033.8906254
CACCGGG2850.033.8906253
ATTGGGC251.6437449E-433.89062533
GATTGGG251.6437449E-433.89062532
CGGGTCA301.0989528E-533.8906256
TTGGGCG200.00243375233.89062534
CGGGATT200.00243375233.8906256
CGGGAAG200.00243375233.8906256
CCGGGTC357.2670264E-733.8906255
Produced by FastQC (version 0.11.5)