FastQCFastQC Report
Fri 17 Jun 2016
SRR1526078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56279
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG13432.3863252723040564No Hit
CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT4800.8528936192896106No Hit
CGCTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3530.6272321825192345No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3040.5401659588834201No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.3393805860089909No Hit
CGCTTGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1890.3358268625952842No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1810.32161196894045735No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1590.28252101138968355No Hit
CGCTTGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1360.24165319213205635No Hit
CGCTTGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1290.22921516018408286No Hit
CGCTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1250.22210771335666948No Hit
CGCTTGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1250.22210771335666948No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1120.19900851116757584No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA1090.19367792604701575No Hit
CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT900.15991755361680202No Hit
CGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC900.15991755361680202No Hit
CGCTTGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT780.13859521313456172No Hit
CGCTTGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA770.13681835142770837No Hit
CGCTTGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC730.12971090460029497No Hit
CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA720.12793404289344162No Hit
CGCTTGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG690.12260345777288154No Hit
CGCTTGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT660.11727287265232147No Hit
CGCTTGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC630.11194228753176141No Hit
CGCTTGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC620.11016542582490804No Hit
CGCTTGGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG620.11016542582490804No Hit
CGCTTGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT610.1083885641180547No Hit
CGCTTGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT610.1083885641180547No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC570.10128111729064126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACAA150.00811261650.42574339
CAACAAA150.00811261650.42574340
TCCAATC150.00811261650.42574340
TGATGTT150.00811261650.42574340
TACTATT150.00828740650.15487746
TATACTA150.00828740650.15487744
CAGAACC150.00834628750.06523513
AGAACCT150.00834628750.06523514
TCGGACG204.1229825E-450.06523514
TCGTGGC150.00834628750.06523515
CTCGGAC204.1229825E-450.06523513
GAGCATA252.0568961E-550.020549
CTGCTCG204.1412254E-450.02053510
CGATTAA150.00837584350.02053549
ACGATTA150.00837584350.02053548
GATGGTT150.00837584350.0205359
ACTAATC204.1412254E-450.02053510
GACGCAC150.00837584350.0205359
GATTCCC150.00837584350.0205359
GGTTAGT150.00837584350.02053549
Produced by FastQC (version 0.11.5)