##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526078_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56279 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.45704436823682 39.0 38.0 40.0 36.0 40.0 2 37.612146626628046 39.0 38.0 40.0 34.0 40.0 3 37.46599975123936 39.0 38.0 40.0 33.0 40.0 4 37.64994047513282 39.0 38.0 40.0 34.0 40.0 5 37.84919774693935 39.0 38.0 40.0 35.0 40.0 6 36.37768972440875 39.0 35.0 40.0 30.0 40.0 7 37.406012900015995 39.0 38.0 40.0 33.0 40.0 8 37.1970184260559 39.0 38.0 40.0 33.0 40.0 9 37.2220366388884 39.0 38.0 40.0 33.0 40.0 10 36.33596901153183 39.0 36.0 40.0 31.0 40.0 11 34.858561808134475 38.0 34.0 40.0 26.0 40.0 12 33.93999538015956 38.0 32.0 40.0 22.0 40.0 13 33.59130403880666 38.0 32.0 39.0 19.0 40.0 14 34.189679987206596 38.0 33.0 40.0 22.0 40.0 15 33.00872439098065 38.0 31.0 39.0 17.0 40.0 16 32.54841059720322 37.0 29.0 39.0 18.0 40.0 17 31.497503509301872 36.0 27.0 39.0 15.0 40.0 18 30.92204907692034 35.0 26.0 39.0 14.0 40.0 19 32.18163080367455 36.0 29.0 39.0 17.0 40.0 20 32.76159846479148 37.0 30.0 39.0 20.0 40.0 21 32.961157803088184 38.0 30.0 39.0 18.0 40.0 22 32.18358535155209 37.0 29.0 39.0 16.0 40.0 23 32.97162351854155 38.0 30.0 39.0 19.0 40.0 24 32.24291476394392 37.0 29.0 39.0 16.0 40.0 25 32.72247197000657 37.0 30.0 39.0 18.0 40.0 26 33.15977540468025 38.0 32.0 39.0 18.0 40.0 27 33.458679081007126 38.0 33.0 39.0 20.0 40.0 28 33.385028163258056 38.0 32.0 39.0 20.0 40.0 29 32.81310968567316 37.0 31.0 39.0 17.0 40.0 30 32.60722471970006 37.0 31.0 39.0 17.0 40.0 31 33.42637573517653 38.0 33.0 39.0 19.0 40.0 32 33.258870982071464 38.0 33.0 39.0 18.0 40.0 33 33.05570461450985 38.0 32.0 39.0 18.0 40.0 34 32.92880115140638 37.0 32.0 39.0 17.0 40.0 35 32.864514294852434 37.0 32.0 39.0 17.0 40.0 36 32.041702944259846 36.0 31.0 39.0 12.0 40.0 37 31.77767906323851 36.0 30.0 38.0 10.0 40.0 38 31.106078643899146 36.0 29.0 38.0 4.0 40.0 39 30.15639936743723 35.0 27.0 38.0 4.0 40.0 40 27.851525435775333 33.0 18.0 38.0 4.0 40.0 41 25.896231276319764 31.0 14.0 37.0 4.0 39.0 42 27.075321167753515 32.0 20.0 37.0 4.0 39.0 43 26.989640896249046 32.0 19.0 38.0 4.0 39.0 44 26.106416247623446 31.0 17.0 37.0 4.0 39.0 45 27.13415305886743 32.0 21.0 38.0 4.0 39.0 46 25.119511718402958 30.0 15.0 36.0 4.0 39.0 47 24.17365269461078 28.0 10.0 35.0 4.0 39.0 48 24.742621581762293 29.0 14.0 36.0 4.0 39.0 49 23.674478935304464 28.0 8.0 35.0 4.0 39.0 50 24.431031112848487 29.0 10.0 36.0 4.0 39.0 51 24.676326871479592 30.0 4.0 36.0 4.0 39.0 52 24.52818991097923 30.0 4.0 36.0 4.0 39.0 53 24.30743261251977 30.0 4.0 36.0 4.0 39.0 54 23.94934167273761 30.0 4.0 36.0 4.0 39.0 55 23.661863217185807 30.0 4.0 36.0 4.0 39.0 56 23.18040476909682 29.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.6468656277298308 1 2 1.5637006836539058 1 3 1.587760628410848 1 4 1.4553051223480153 1 5 1.485684282916921 1 6 3.3423530132433683 1 7 2.129921428054949 1 8 2.2995985618070662 1 9 2.264042915519944 1 10 3.2717296763282135 1 11 -0.16019182729167625 1 12 -6.243699106727071 1 13 -17.70027343237301 1 14 -5.369944194868236 1 15 1.6468261484763396 1 16 0.2237271372698615 1 17 -4.733119842425303 1 18 -2.99078115934644 1 19 0.7457730430615648 1 20 0.2260168815777419 1 21 6.573483929651271 1 22 7.125232911833855 1 23 6.779630055535357 1 24 7.423681740014132 1 25 6.313857988472936 1 26 6.764644667158002 1 27 5.945253940595599 1 28 6.102199409692904 1 29 6.688022603572456 1 30 6.44721699802086 1 31 5.66159049666528 1 32 5.664498814049203 1 33 5.631886404224737 1 34 5.461201453661733 1 35 5.850270858727335 1 36 5.59293325606658 1 37 5.841472013921205 1 38 6.381314163913565 1 39 6.487866187813822 1 40 -8.077720948035243 1 41 2.3279845022154255 1 42 0.33475078621236065 1 43 -7.345922767312185 1 44 -13.873267236177423 1 45 0.2855598661881551 1 46 -3.6863934884427785 1 47 -8.087035810880812 1 48 -4.116545675480271 1 49 -12.406376890413085 1 50 -2.500356908841212 1 51 3.775271777692044 1 52 3.7705359953944075 1 53 3.6741662304144747 1 54 3.7346221371870953 1 55 3.549646521217081 1 56 5.888709254872865 2 1 0.7751123809765801 2 2 1.6692201641733888 2 3 1.5455528362030577 2 4 1.7150453820882703 2 5 1.6220479192805612 2 6 3.5160543119446643 2 7 1.6250512981848217 2 8 2.3125855747940847 2 9 2.4296273311043564 2 10 3.3399114945100337 2 11 3.9924055753057246 2 12 0.8959112828833184 2 13 1.6438824117828403 2 14 4.621938922014884 2 15 5.554293680943868 2 16 5.0467790853218055 2 17 7.158113923808465 2 18 5.871231827666545 2 19 6.645123692412216 2 20 5.66757532313618 2 21 7.4468605530279 2 22 7.993739405340349 2 23 7.222811873717177 2 24 6.900954467286859 2 25 7.028143702758651 2 26 5.904255056768392 2 27 6.112461732803389 2 28 4.933368240861736 2 29 6.387697928247775 2 30 6.429359855163714 2 31 4.723278808353594 2 32 5.278135177685563 2 33 4.39325004058837 2 34 5.206331323791606 2 35 5.363257871714353 2 36 4.571829359962685 2 37 5.646666819116007 2 38 5.65241806001746 2 39 6.411567486515125 2 40 4.415785545471252 2 41 9.266296190527111 2 42 7.786049487511065 2 43 5.949531778142362 2 44 -3.298591911502097 2 45 6.761209216837507 2 46 6.15613897908969 2 47 2.924327825482827 2 48 6.183778999844407 2 49 -1.5833249423611377 2 50 6.153863870379567 2 51 9.098323725743992 2 52 9.419886644745056 2 53 9.057283113531359 2 54 9.619362396927357 2 55 9.470101066671624 2 56 9.758839125002737 3 1 0.36374874461294837 3 2 0.8714928914461169 3 3 0.6773710180212404 3 4 1.1948433618862495 3 5 1.1988155960482345 3 6 2.3077209786113286 3 7 1.4283341264676537 3 8 1.5166259788344902 3 9 1.6200313715083965 3 10 2.157588262186806 3 11 3.815132848032995 3 12 4.207527444499483 3 13 4.691357159257585 3 14 4.201484376560337 3 15 -0.4813123796621923 3 16 5.396021509564232 3 17 6.025033115727652 3 18 7.61340354483826 3 19 5.9830024802910025 3 20 5.66454502010588 3 21 6.394840351007698 3 22 6.962173748774688 3 23 5.976599752505059 3 24 6.080247396579793 3 25 5.656426531041479 3 26 5.8219318244451586 3 27 5.238471833813492 3 28 5.208115715609214 3 29 5.492495908045754 3 30 5.604359855163711 3 31 4.243985879060663 3 32 4.4139937635441555 3 33 4.740977313315646 3 34 4.5707252631855475 3 35 5.039015447471925 3 36 6.000869764003085 3 37 5.181010253459441 3 38 6.238024120623521 3 39 7.890607890555529 3 40 8.95618958587529 3 41 11.772861847092766 3 42 10.046150497612075 3 43 9.740945919556502 3 44 10.012014149103965 3 45 8.883683964312258 3 46 10.922553120503832 3 47 11.148317724472726 3 48 9.832516373581779 3 49 11.784099300063108 3 50 10.246540638056338 3 51 10.961707564127831 3 52 10.516856341714753 3 53 11.232030588278832 3 54 11.593352295917256 3 55 11.218333389903947 3 56 9.924495690659302 4 1 0.9049252152011817 4 2 1.3950223032108227 4 3 1.3656063121388868 4 4 1.6412485906444232 4 5 1.2589463150024827 4 6 2.8528190178270165 4 7 1.8727785709120965 4 8 2.24734493308285 4 9 1.7683973845802967 4 10 2.705954275258698 4 11 4.644544612738883 4 12 5.20687384972824 4 13 5.886128401087653 4 14 5.354425553030929 4 15 5.228491541906433 4 16 6.992099940936779 4 17 8.61065403076033 4 18 8.892488512158526 4 19 7.320257382251786 4 20 6.595917569125486 4 21 6.141245579765869 4 22 7.637990742238742 4 23 6.684442889759957 4 24 7.5220774619392685 4 25 6.700870975485923 4 26 6.064415484575882 4 27 5.906445690022643 4 28 5.520534016262811 4 29 6.15066584268628 4 30 5.716124561046069 4 31 4.166861696054131 4 32 4.709418600145462 4 33 4.811565548609764 4 34 3.686411537695353 4 35 3.9135252513934944 4 36 4.043353424133805 4 37 4.8980037175117275 4 38 5.734102551996067 4 39 4.956620962450948 4 40 0.7516144224765995 4 41 9.467633088922835 4 42 8.572947883232992 4 43 7.97493284766108 4 44 8.143386698123571 4 45 8.072572853201141 4 46 11.180069460373115 4 47 10.674461515322399 4 48 10.353431406261517 4 49 11.533118907906246 4 50 11.00078900406941 4 51 10.580008217722604 4 52 10.359993596616714 4 53 9.6268018301089 4 54 9.401849027943399 4 55 9.017026200361464 4 56 8.154561050136426 5 1 0.46745244831664934 5 2 1.0554435087300647 5 3 0.7798401538237059 5 4 1.3368186705282312 5 5 0.8605439911099637 5 6 2.4311777687347913 5 7 1.643148941282469 5 8 1.543786472661644 5 9 1.929907914718278 5 10 2.141538879470751 5 11 4.268219267786087 5 12 5.22110769141306 5 13 5.446912714813138 5 14 4.979262154338116 5 15 6.46436663268349 5 16 6.707132620675342 5 17 7.706514597209136 5 18 8.614638112739495 5 19 7.750903714858904 5 20 6.8707178596120535 5 21 6.883729239896585 5 22 7.665877452478398 5 23 6.5136367895420975 5 24 6.907407890406951 5 25 6.8811178890661715 5 26 5.92193182444516 5 27 5.048348377023366 5 28 5.371078678572179 5 29 6.103607019156868 5 30 5.131520348990872 5 31 5.167442669184123 5 32 4.712759195642917 5 33 5.022458794797124 5 34 4.764552423679373 5 35 4.9081512499410636 5 36 5.607042603509257 5 37 7.105701611484136 5 38 6.3009870835864845 5 39 6.894311594259229 5 40 9.19693032661603 5 41 10.351874192771785 5 42 9.675780127241701 5 43 10.22983480844539 5 44 11.104606741696553 5 45 9.754054334682625 5 46 11.194158058775432 5 47 12.05325599607766 5 48 10.376960818026223 5 49 10.216198065495206 5 50 11.219380144229177 5 51 8.722201391288323 5 52 9.857597082455495 5 53 9.550549106797352 5 54 9.658784394682687 5 55 9.060308698545924 5 56 9.484989517819795 6 1 0.05643167144221195 6 2 0.9666148426656278 6 3 1.0627368716797747 6 4 0.6718081315339504 6 5 0.6131787396796753 6 6 2.116664068042226 6 7 1.7727785709120951 6 8 1.567574488319579 6 9 1.339272563920325 6 10 1.6597562838670186 6 11 3.807815774862263 6 12 5.150345872683765 6 13 5.365611901805011 6 14 4.969777059487164 6 15 5.598362417085777 6 16 6.3358589079382135 6 17 7.108501950415999 6 18 8.317739588198695 6 19 7.276498415250355 6 20 5.323894613601812 6 21 6.654731949923683 6 22 6.463528762324827 6 23 5.777412760635137 6 24 5.786209456200389 6 25 5.59355390231519 6 26 5.065563260759525 6 27 4.282916278257936 6 28 4.2970046044981025 6 29 4.310924092327603 6 30 4.4628964405295655 6 31 3.0941213804156753 6 32 3.6600642242487567 6 33 3.382440727949792 6 34 2.9455220111530274 6 35 3.4552756100735493 6 36 4.281086566171112 6 37 4.594560388960801 6 38 4.565666397046286 6 39 5.480580790284527 6 40 3.051040534384775 6 41 9.427604394518244 6 42 7.86864372254432 6 43 8.502192532022626 6 44 9.45916862064868 6 45 8.394524072713342 6 46 10.656970464677276 6 47 10.955092792223404 6 48 9.840375452172562 6 49 10.658083039900507 6 50 10.019440367053626 6 51 8.462791574967937 6 52 8.344498618137518 6 53 8.518480452777478 6 54 8.351346875863054 6 55 8.847872685296903 6 56 8.371921164914045 7 1 0.7149682568080706 7 2 1.3812489890070907 7 3 1.2456637009480644 7 4 1.3425398388510246 7 5 1.3448860567528413 7 6 2.860566507066615 7 7 1.7971688148145333 7 8 1.9700135127098264 7 9 2.180735978554466 7 10 3.31829286923287 7 11 4.807815774862263 7 12 4.882053189756931 7 13 5.621709462780622 7 14 5.823435596072535 7 15 6.281289246354067 7 16 7.226102810377238 7 17 8.242648291879412 7 18 8.781154222345041 7 19 7.032595976225959 7 20 7.421455589211572 7 21 6.508390486509054 7 22 7.695236079398001 7 23 7.057900565513187 7 24 7.603282626932092 7 25 6.349651463290801 7 26 6.346051065637575 7 27 5.465843107526226 7 28 5.528711921571276 7 29 5.713363116717844 7 30 6.292164733212491 7 31 5.191682356025435 7 32 5.001527638882905 7 33 5.138538288925396 7 34 5.128448840421317 7 35 5.052836585683309 7 36 5.98840363934184 7 37 6.143340876765677 7 38 7.651032250704823 7 39 8.004971034186966 7 40 8.026650290482333 7 41 12.22028732134751 7 42 11.319863234739444 7 43 10.868046190559213 7 44 11.422583254795018 7 45 11.248182609298706 7 46 13.01062900126264 7 47 12.394117182467305 7 48 11.376960818026223 7 49 13.29222938136392 7 50 13.348708659736552 7 51 12.316450111553305 7 52 12.44205959374727 7 53 12.420919477167722 7 54 13.326956631960613 7 55 11.66494585602861 7 56 9.640213847840872 8 1 0.8534923343565382 8 2 1.527903147856371 8 3 1.5158325564827777 8 4 1.7033903704332616 8 5 1.4740292712619123 8 6 3.051310722201073 8 7 1.9522657503992775 8 8 2.0328652950738046 8 9 2.318321969798994 8 10 3.391776296374836 8 11 5.027953360853509 8 12 5.713510350482387 8 13 6.062297330197758 8 14 5.855330530406491 8 15 6.644328645978835 8 16 7.35243176597449 8 17 8.73358012427466 8 18 9.204856536291256 8 19 7.580438377726903 8 20 6.6594168149776785 8 21 7.029028385195726 8 22 7.611746398347343 8 23 7.002240778146081 8 24 7.264008080340474 8 25 6.775229949844896 8 26 6.481760884274223 8 27 6.0521470474887025 8 28 5.7841840916775915 8 29 6.257453173003022 8 30 6.522308573112429 8 31 4.462789297864081 8 32 5.39177154132193 8 33 5.105079877418206 8 34 5.3348278272881124 8 35 5.400553909010391 8 36 6.468391131524456 8 37 6.051095723544911 8 38 6.504690787290187 8 39 6.8017190016666405 8 40 2.9390955687812728 8 41 10.587391761622687 8 42 9.695723147184722 8 43 9.140091218701805 8 44 10.024834661924476 8 45 9.272572853201144 8 46 11.00374970170041 8 47 12.119257895412893 8 48 11.19747363853904 8 49 11.002048018011823 8 50 10.324318415834114 8 51 11.54034004276031 8 52 10.463010187868601 8 53 10.023483579731824 8 54 10.743779646344606 8 55 10.281581253151813 8 56 10.469794835958446 9 1 0.39666013701801006 9 2 1.1259232711929528 9 3 1.3064849420718687 9 4 1.7932962451492642 9 5 1.1588718548386723 9 6 2.311940387894033 9 7 1.7537912291399422 9 8 1.5197202123084637 9 9 1.8522395290330138 9 10 3.0910622424962284 9 11 5.3758923417038815 9 12 5.0642081758637545 9 13 5.818220731690779 9 14 5.125535009471733 9 15 6.47733740936734 9 16 7.022181847116972 9 17 8.42897123105817 9 18 8.332250239634323 9 19 7.381314716577922 9 20 6.524882572848494 9 21 6.397090702625139 9 22 7.364424100392135 9 23 6.662253760943877 9 24 7.437490715848426 9 25 6.0350481906757985 9 26 4.712367830071038 9 27 4.624688430156667 9 28 5.664093212093039 9 29 5.331455120422689 9 30 5.45625858934093 9 31 4.534843825614811 9 32 3.746201921068767 9 33 4.2371798449612115 9 34 4.325577583860653 9 35 4.481209540298927 9 36 4.777381718996054 9 37 5.065539086089544 9 38 5.903424964505376 9 39 6.221550225295331 9 40 1.3858660978302844 9 41 7.574408963829757 9 42 8.110848106613481 9 43 8.791016243044545 9 44 8.938174486656703 9 45 7.449788043074559 9 46 9.967560152852638 9 47 11.2317213812941 9 48 9.376960818026223 9 49 10.969753308501925 9 50 9.052166517099938 9 51 8.805026832763552 9 52 8.125295160280153 9 53 8.642438464509492 9 54 8.993211648941166 9 55 8.716504979214779 9 56 9.909587111193758 10 1 0.7042249350891367 10 2 1.7714928914461154 10 3 1.751180541830763 10 4 1.722621139664028 10 5 1.505164802397445 10 6 3.3434352643256133 10 7 2.165635713769241 10 8 2.0539275661360747 10 9 1.9859043873814173 10 10 3.2901279447264855 10 11 4.824656657556808 10 12 5.418638555610592 10 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2.262817020356735 114 11 0.1425838284251526 114 12 -3.5617536012521533 114 13 -4.906028461657449 114 14 -6.876946995988682 114 15 -9.371508458093565 114 16 -3.972605941416159 114 17 0.8067324621328815 114 18 0.3669983160800925 114 19 -0.22876222559134973 114 20 -0.6354549798941207 114 21 3.2824220503541035 114 22 4.543873095179919 114 23 3.5550311250540787 114 24 4.576979422723582 114 25 4.242047446074157 114 26 4.111474308105294 114 27 3.9691907880618587 114 28 3.963671271164767 114 29 4.005567803470591 114 30 4.618085345359791 114 31 4.213920519583542 114 32 3.685889188380756 114 33 4.79587927409996 114 34 4.388372322009072 114 35 4.752740937668001 114 36 5.545314208447529 114 37 5.701925286139179 114 38 6.592926081407832 114 39 6.105640570294092 114 40 8.782986971496204 114 41 9.032338971275774 114 42 7.137653765585931 114 43 7.680815200602254 114 44 0.257112188319649 114 45 7.17453363751487 114 46 0.6153635780201689 114 47 -3.976518876834465 114 48 1.2593137592026942 114 49 -6.470802660721208 114 50 1.0596125334811717 114 51 1.9800082177226024 114 52 1.528621047597106 114 53 2.254252810501054 114 54 1.52733922402183 114 55 2.8170262003614646 114 56 6.134953206999171 115 1 0.1411072351789855 115 2 1.0922476084272503 115 3 0.920767244436334 115 4 0.7493506994124601 115 5 0.9350839398637518 115 6 2.52784676477485 115 7 1.4803257407234156 115 8 1.7396867754801875 115 9 1.8374317907956126 115 10 2.6542339644928816 115 11 0.6283403952028124 115 12 -2.6568331425026166 115 13 -2.6159803669478663 115 14 -5.45889298193023 115 15 -7.585714895385461 115 16 1.1117447800044822 115 17 2.3487228431909664 115 18 3.7783930626579725 115 19 2.7741974488444647 115 20 1.9934758377159447 115 21 4.2757627828735245 115 22 5.507247124036745 115 23 5.105530570115121 115 24 4.98800420999698 115 25 5.244267201901021 115 26 4.9449926210908615 115 27 4.528199297125859 115 28 4.089457434686782 115 29 3.6319089059493237 115 30 5.115680609880695 115 31 3.551323405266114 115 32 3.976677201699289 115 33 4.352298068032624 115 34 3.773869917273597 115 35 4.413858214767522 115 36 6.350345654988409 115 37 6.173253440042252 115 38 7.4763889004977315 115 39 6.308008309842741 115 40 10.27170321270967 115 41 9.731562056736376 115 42 8.283047772245197 115 43 6.261281349325895 115 44 -0.7458474861161619 115 45 6.329176626786051 115 46 2.5410017578203927 115 47 -6.411221059594361 115 48 1.1128098746299955 115 49 -3.8133849766664554 115 50 2.8054504913058125 115 51 4.092105886978981 115 52 4.613711687626701 115 53 5.713372307356401 115 54 3.354568228831301 115 55 6.149989574390322 115 56 6.741686466340642 116 1 0.43041541127961125 116 2 0.898477018430242 116 3 1.1837202243704468 116 4 0.917065584108478 116 5 1.2591330563656982 116 6 2.434705105595455 116 7 1.8124611105946329 116 8 1.8436101058186125 116 9 1.8946345461115754 116 10 1.809969214567758 116 11 3.0389423718425235 116 12 2.529749666721706 116 13 4.208817476717904 116 14 3.5665637416397047 116 15 -4.405121903471716 116 16 4.278561192103918 116 17 6.240906131600667 116 18 5.745149576584293 116 19 5.690938988227511 116 20 4.6978783534392115 116 21 5.851983208150553 116 22 6.279634066235012 116 23 5.46072673663204 116 24 5.826279142611533 116 25 5.919918594533545 116 26 4.429868332381666 116 27 4.489265484607145 116 28 4.090655398148893 116 29 4.690908606458457 116 30 4.209121759925619 116 31 3.4249382600130467 116 32 4.217168366718752 116 33 3.175897948236276 116 34 4.223106215566499 116 35 3.3120313204877974 116 36 5.529441192574517 116 37 5.334978507427699 116 38 5.7824685650679655 116 39 6.571560271507906 116 40 9.297459427145132 116 41 8.78397295820388 116 42 7.062023513485091 116 43 1.118723697334282 116 44 5.067569704659519 116 45 6.986858567486859 116 46 2.5257277236784326 116 47 -5.885015608860609 116 48 1.8055322465976502 116 49 0.27616279212659833 116 50 1.967175558691256 116 51 6.561707564127829 116 52 5.942253167111577 116 53 6.524094080342326 116 54 5.750495153060111 116 55 5.211984183554744 116 56 7.641956008119617 117 1 -0.1962512553870539 117 2 0.8114928914461146 117 3 1.057371018021236 117 4 0.9259544729973612 117 5 0.6965933738260119 117 6 2.6410543119446643 117 7 1.2127785709120928 117 8 1.5144037566122677 117 9 1.1778091492861762 117 10 2.4353660399645776 117 11 -0.6048671519669995 117 12 -8.241361444389408 117 13 -7.559753951853526 117 14 -2.6385156234396625 117 15 -9.127979046328857 117 16 -7.517311823769102 117 17 -0.6105224398279034 117 18 -2.5710408996061815 117 19 -0.8303308530423301 117 20 -1.508788313227452 117 21 3.0659514621188073 117 22 5.393284859885803 117 23 4.463266419171724 117 24 5.129136285468675 117 25 4.380870975485923 117 26 4.839709602222939 117 27 4.1829162782579346 117 28 4.177004604498105 117 29 4.823607019156867 117 30 5.484359855163714 117 31 3.6950969901717734 117 32 2.8117715413219315 117 33 4.180977313315644 117 34 3.7040585965188804 117 35 4.072348780805257 117 36 5.458647541780863 117 37 5.552121364570553 117 38 5.584690787290185 117 39 6.4083856683333025 117 40 7.873967363653069 117 41 6.7417507359816575 117 42 5.790594942056519 117 43 -3.556831858221276 117 44 -1.0213191842293696 117 45 5.112572853201144 117 46 -1.5552246572739463 117 47 -0.7361267199717183 117 48 0.9169608180262223 117 49 -2.9492340332702263 117 50 1.3443184158341133 117 51 4.930596453016719 117 52 6.123523008381419 117 53 5.740919477167722 117 54 7.4089078514728115 117 55 6.480555612126171 117 56 10.21338457954819 118 1 -0.8953421644779596 118 2 -0.13759801764479107 118 3 -0.31808352743330914 118 4 -0.3985909815480895 118 5 -0.10522480799216538 118 6 1.5728724937628442 118 7 0.34096038909391524 118 8 0.4489492111577178 118 9 0.42962733110435636 118 10 1.726275130873674 118 11 -0.6666853337851819 118 12 -5.126815989843955 118 13 -5.33339031548989 118 14 -8.86669744162148 118 15 -10.388888137237949 118 16 -11.964584551041828 118 17 -1.0805224398279023 118 18 -6.731040899606182 118 19 -1.3094217621332405 118 20 -1.6506064950456363 118 21 4.49231509848244 118 22 5.6869212235221696 118 23 4.7569027828080905 118 24 5.673681740014132 118 25 3.573598248213198 118 26 4.245164147677485 118 27 4.1238253691670295 118 28 3.274277331770829 118 29 4.149061564611415 118 30 3.008905309709167 118 31 2.382369717444501 118 32 2.1872260867764766 118 33 2.1091591314974636 118 34 3.1949676874279724 118 35 2.2723487808052596 118 36 4.355920269053591 118 37 4.66939409184328 118 38 4.4819635145629135 118 39 6.172931122878758 118 40 8.211240090925795 118 41 5.379932554163474 118 42 4.876958578420155 118 43 -0.027740949130365067 118 44 0.792317179406993 118 45 4.045300125928417 118 46 -2.2188610209103103 118 47 -1.9733994472444465 118 48 -2.23667554561014 118 49 -2.6401431241793176 118 50 0.39250023401593026 118 51 4.018778271198535 118 52 4.601704826563235 118 53 3.6481922044404484 118 54 5.221635124200084 118 55 4.5723737939443545 118 56 7.71338457954819 119 1 -0.41482268395848365 119 2 0.07506432001754604 119 3 0.5845138751640917 119 4 0.5202401872830791 119 5 0.1658790881117298 119 6 2.2660543119446643 119 7 1.1120642851978104 119 8 1.1372608994694104 119 9 1.051380577857607 119 10 1.6175088971074345 119 11 0.640132848032998 119 12 -3.938504301532266 119 13 -2.5054682375678112 119 14 -5.816372766296805 119 15 -9.780121903471716 119 16 -9.094454680911959 119 17 0.18733470302924005 119 18 -7.088183756749039 119 19 0.08181200410052725 119 20 -0.4509311703703105 119 21 4.6059514621188065 119 22 5.805427717028657 119 23 4.696837847743154 119 24 4.058421999754394 119 25 4.925870975485925 119 26 5.253281030794369 119 27 4.407916278257936 119 28 3.7970046044981025 119 29 5.407178447728299 119 30 4.929359855163714 119 31 3.2443827044574896 119 32 2.713200112750499 119 33 3.5588344561727894 119 34 3.5147728822331636 119 35 3.8080630665195443 119 36 5.21793325606658 119 37 5.227835650284838 119 38 5.861833644433041 119 39 6.825528525476159 119 40 9.162538792224499 119 41 7.8296078788388 119 42 5.919166370627945 119 43 -2.2939747153641328 119 44 -2.0177477556579433 119 45 5.147572853201144 119 46 -1.55165322870252 119 47 -2.5318410056860046 119 48 -4.19446775340235 119 49 -0.19209117612737003 119 50 1.0564612729769713 119 51 5.736310738731003 119 52 5.854951579809992 119 53 5.992348048596295 119 54 4.984622137187095 119 55 6.158412754983313 119 56 7.641956008119617 120 1 0.018650705397263323 120 2 0.751885048308857 120 3 0.8597239591977086 120 4 0.5902681984875571 120 5 0.7138482757868019 120 6 2.444975880572116 120 7 1.3512099434611144 120 8 1.1610704232789288 120 9 1.2036915022273504 120 10 2.0079150595724258 120 11 -0.9750632303983764 120 12 -7.4244986992913695 120 13 -6.670734344010388 120 14 -6.210280329322014 120 15 -0.7636653208386619 120 16 -7.952998098278904 120 17 -0.5462087143377055 120 18 -4.829080115292456 120 19 -0.42484065696389806 120 20 -1.4393765485215688 120 21 2.6745789130992 120 22 3.739951526552467 120 23 3.3589526936815304 120 24 3.9495284423314203 120 25 3.144008230387886 120 26 4.856572347320977 120 27 4.498602552767743 120 28 4.512690879007909 120 29 5.0447834897451 120 30 4.55926181594803 120 31 4.233528362720794 120 32 3.823144090341536 120 33 4.050781234884269 120 34 4.44719585142084 120 35 3.1056821141385953 120 36 4.957078914329884 120 37 5.250944893982322 120 38 6.259592748074503 120 39 7.693875864411737 120 40 8.763379128358952 120 41 -4.457857107155597 120 42 -1.6858756461787756 120 43 -6.828988720966372 120 44 -13.958574086190154 120 45 -2.8058585193478756 120 46 -7.9140481866857115 120 47 -6.074558092520739 120 48 -4.328921534914954 120 49 -14.92178305287807 120 50 -3.881563937107064 120 51 1.6270670412520118 120 52 1.8619543809304382 120 53 1.6071939869716445 120 54 1.6057705965708493 120 55 2.3856536513418583 120 56 5.468286540332503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 235.0 5 45.0 6 385.0 7 233.0 8 412.0 9 652.0 10 225.0 11 225.0 12 252.0 13 133.0 14 177.0 15 199.0 16 227.0 17 275.0 18 379.0 19 480.0 20 630.0 21 770.0 22 1021.0 23 1250.0 24 1482.0 25 1688.0 26 1892.0 27 2038.0 28 1944.0 29 1993.0 30 2330.0 31 2631.0 32 2952.0 33 3275.0 34 3416.0 35 3951.0 36 4136.0 37 4502.0 38 4825.0 39 5018.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.3905364345493 0.4868601076778194 0.07462819168784093 0.0479752660850406 8 98.68867606034223 1.0003731409584393 0.17057872385792214 0.1403720748414151 9 93.97821567547398 2.365002931821816 2.4822758044741375 1.1745055882300681 10 42.73174718811635 28.243216830434086 18.39584925105279 10.629186730396773 11 30.11602906945752 29.655821887382505 18.593080900513513 21.635068142646457 12 27.432967892108955 21.38808436539384 20.325521064695533 30.853426677801664 13 21.35077026954992 17.926757760443504 22.77048277332575 37.95198919668083 14 21.49114234439134 29.302226407718692 26.645818155972922 22.560813091917055 15 33.062398399399775 21.28298111791922 21.224030439986425 24.430590042694583 16 30.535301440512182 27.41063489240619 22.271029699448693 19.78303396763294 17 25.243926280057583 20.390282048092132 33.46899603675333 20.89679563509695 18 21.930027185984116 20.76262904458146 27.6408607118108 29.666483057623626 19 28.191224576873847 22.601692504622388 21.054970843407766 28.152112075096003 20 23.864161952538513 27.553634325968616 26.466716476322627 22.115487245170243 21 23.30354128538176 20.71465377849642 27.955365233923846 28.02643970219798 22 20.536967607811086 20.058991808667532 29.797970823930775 29.606069759590607 23 19.952380106256328 23.82949235061035 26.914124273707777 29.30400326942554 24 19.605508662816526 23.589515770768546 28.250555308751668 28.55442025766326 25 29.441137272323413 24.602398933807194 25.434029320302088 20.5224344735673 26 29.841433498062358 24.02318057382586 19.30351619440395 26.831869733707826 27 22.495465699349197 28.217575304953947 19.033393790675344 30.253565205021516 28 26.50733007552199 30.81830297645491 21.019991115059973 21.654375832963126 29 32.967287224575514 24.436692414480476 20.807674509664327 21.78834585127968 30 27.298748399943108 22.969705589532072 28.767245057602047 20.96430095292277 31 26.834079761143098 31.486813108694108 21.70149996445582 19.977607165706974 32 32.73496374235746 25.814019621783025 21.093416749608988 20.357599886250533 33 26.37253157801103 26.159046433019036 21.343177370574633 26.125244618395303 34 22.76513937840436 28.851151696393607 28.66424579016697 19.719463135035067 35 22.78381889693712 24.704123582907688 33.638256598266565 18.873800921888627 36 26.301194539249146 28.247653583617748 24.425839021615474 21.025312855517633 37 25.959142999501744 22.7685244501388 30.655206776283013 20.61712577407645 38 32.14451052312952 24.153342117445963 23.665467753997365 20.036679605427157 39 26.159376445883904 23.358365661814428 21.85642595294871 28.62583193935296 40 21.399634316853692 18.95099128813681 28.30997024343025 31.33940415157925 41 19.8544474393531 16.59299191374663 24.878706199460918 38.67385444743935 42 25.835709194583035 26.819315751960087 23.232359230220958 24.112615823235924 43 18.879024494598355 23.041680037080614 30.891004385495773 27.188291082825256 44 21.436479874281225 24.86874531233258 24.720525733061898 28.9742490803243 45 24.141308025142003 23.29018357935222 30.882640978615054 21.685867416890726 46 19.884438420658416 27.401291150979063 24.697720868851874 28.016549559510644 47 17.956827847467924 27.42039240913477 24.291483506665237 30.33129623673207 48 23.52020767767287 28.082715456695297 23.294393769669814 25.10268309596202 49 19.24749581149966 29.01293979253556 22.685630770327595 29.053933625637185 50 25.59555603033864 26.25253712210234 24.61987679378984 23.532030053769184 51 21.253342841861294 30.039222677839188 25.879835977892675 22.827598502406847 52 21.20899268409904 28.515993520710587 29.331244771177843 20.943769024012532 53 21.092956541881225 26.851736364770236 28.93287956809151 23.122427525257024 54 22.323019890211736 28.43979468168532 26.46324944749412 22.773935980608826 55 19.746573755591594 27.82163925077079 28.03906542389193 24.39272156974568 56 25.465528608849052 23.042106951299914 28.104563516812487 23.387800923038544 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 3.0 9 5.0 10 35.5 11 66.0 12 191.0 13 328.5 14 341.0 15 277.0 16 213.0 17 184.5 18 156.0 19 163.5 20 171.0 21 177.0 22 186.0 23 189.0 24 180.5 25 172.0 26 190.0 27 208.0 28 242.5 29 292.0 30 307.0 31 329.0 32 351.0 33 364.5 34 378.0 35 467.0 36 556.0 37 667.5 38 821.0 39 863.0 40 888.5 41 914.0 42 1051.0 43 1188.0 44 1309.5 45 1431.0 46 1995.0 47 3866.0 48 5173.0 49 4913.0 50 4653.0 51 4067.5 52 3482.0 53 3311.0 54 3228.0 55 3316.0 56 3430.5 57 3545.0 58 3281.5 59 3018.0 60 3074.5 61 3131.0 62 3246.5 63 3118.5 64 2875.0 65 2625.5 66 2376.0 67 2193.5 68 2011.0 69 1677.0 70 1343.0 71 1202.5 72 906.5 73 751.0 74 688.0 75 625.0 76 450.5 77 276.0 78 228.5 79 157.0 80 133.0 81 144.0 82 155.0 83 98.0 84 41.0 85 23.0 86 5.0 87 2.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5330585120560066 16 0.08706622363581443 17 0.021322340482240267 18 0.0 19 0.05508271291245402 20 0.11549601094546812 21 0.0 22 0.0 23 0.0 24 0.007107446827413422 25 0.007107446827413422 26 0.04442154267133389 27 0.07285132998098758 28 0.007107446827413422 29 0.16524813873736208 30 0.05508271291245402 31 0.019545478775386912 32 0.02665292560280033 33 0.12260345777288154 34 0.17946303239218891 35 0.15991755361680202 36 0.040867819257627176 37 0.14747952166882852 38 0.20789281970184262 39 0.1510332450825352 40 0.8759928214787043 41 1.1176460136107607 42 0.28252101138968355 43 0.3287194157678708 44 0.4992981396257929 45 0.20966968140869596 46 0.3642566499049379 47 0.5632651610725137 48 0.06752074486042751 49 0.30739707528563054 50 0.20078537287442919 51 0.3358268625952842 52 0.17768617068533557 53 0.27719042626912344 54 0.30384335187192385 55 0.29851276675136373 56 0.28429787309653687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 56279.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.80260843298566 #Duplication Level Percentage of deduplicated Percentage of total 1 89.41200090345572 63.30602889177135 2 6.26395964564459 8.87009364061195 3 1.7667578487715514 3.752731924874287 4 0.7578989635355234 2.1464489418788535 5 0.4592566567119231 1.6258284617708205 6 0.2509599217005044 1.0661170241120133 7 0.19574873892639344 0.9701664919419323 8 0.1304991592842623 0.7391744700509959 9 0.12798956006725726 0.8155795234456902 >10 0.5521118277411098 7.501910126334867 >50 0.04768238512309584 2.2121928250324276 >100 0.03262478982106558 4.607402405870751 >500 0.0 0.0 >1k 0.002509599217005044 2.3863252723040564 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1343 2.3863252723040564 No Hit CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 480 0.8528936192896106 No Hit CGCTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 353 0.6272321825192345 No Hit CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 304 0.5401659588834201 No Hit CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.3393805860089909 No Hit CGCTTGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 189 0.3358268625952842 No Hit CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 181 0.32161196894045735 No Hit CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 159 0.28252101138968355 No Hit CGCTTGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 136 0.24165319213205635 No Hit CGCTTGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 129 0.22921516018408286 No Hit CGCTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 125 0.22210771335666948 No Hit CGCTTGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 125 0.22210771335666948 No Hit CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 112 0.19900851116757584 No Hit CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA 109 0.19367792604701575 No Hit CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 90 0.15991755361680202 No Hit CGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 90 0.15991755361680202 No Hit CGCTTGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 78 0.13859521313456172 No Hit CGCTTGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 77 0.13681835142770837 No Hit CGCTTGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 73 0.12971090460029497 No Hit CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 72 0.12793404289344162 No Hit CGCTTGGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 69 0.12260345777288154 No Hit CGCTTGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 66 0.11727287265232147 No Hit CGCTTGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 63 0.11194228753176141 No Hit CGCTTGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC 62 0.11016542582490804 No Hit CGCTTGGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 62 0.11016542582490804 No Hit CGCTTGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT 61 0.1083885641180547 No Hit CGCTTGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT 61 0.1083885641180547 No Hit CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 57 0.10128111729064126 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.01243803194797349 0.0 0.0 0.0 0.0 11 0.014214893654826845 0.0 0.0 0.0 0.0 12 0.019545478775386912 0.0 0.0 0.0 0.0 13 0.023099202189093622 0.0 0.0 0.0 0.0 14 0.04442154267133389 0.0 0.0 0.0 0.0 15 0.046198404378187244 0.0 0.0 0.0 0.0 16 0.0479752660850406 0.0 0.0 0.0 0.0 17 0.04975212779189396 0.0 0.0 0.0 0.0 18 0.05152898949874731 0.0 0.0 0.0 0.0 19 0.05685957461930738 0.0 0.0 0.0 0.0 20 0.06219015973986745 0.0 0.0 0.0 0.0 21 0.06929760656728087 0.0 0.0 0.0 0.0 22 0.07462819168784093 0.0 0.0 0.0 0.0 23 0.07640505339469429 0.0 0.0 0.0 0.0 24 0.08173563851525435 0.0 0.0 0.0 0.0 25 0.08706622363581443 0.0 0.0 0.0 0.0 26 0.09239680875637449 0.0 0.0 0.0 0.0 27 0.1083885641180547 0.0 0.0 0.0 0.0 28 0.11371914923861476 0.0 0.0 0.0 0.0 29 0.11549601094546812 0.0 0.0 0.0 0.0 30 0.11904973435917482 0.0 0.0 0.0 0.0 31 0.1243803194797349 0.0 0.0 0.0 0.0 32 0.1243803194797349 0.0 0.0 0.0 0.0 33 0.1279340428934416 0.0 0.0 0.0 0.0 34 0.13504148972085503 0.0 0.0 0.0 0.0 35 0.14214893654826846 0.0 0.0 0.0 0.0 36 0.1439257982551218 0.0 0.0 0.0 0.0 37 0.1439257982551218 0.0 0.0 0.0 0.0 38 0.14570265996197515 0.0 0.0 0.0 0.0 39 0.14570265996197515 0.0 0.0 0.0 0.0 40 0.14747952166882852 0.0 0.0 0.0 0.0 41 0.15281010678938858 0.0 0.0 0.0 0.0 42 0.15991755361680202 0.0 0.0 0.0 0.0 43 0.16169441532365536 0.0 0.0 0.0 0.0 44 0.17235558556477548 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAACAA 15 0.008112616 50.425743 39 CAACAAA 15 0.008112616 50.425743 40 TCCAATC 15 0.008112616 50.425743 40 TGATGTT 15 0.008112616 50.425743 40 TACTATT 15 0.008287406 50.154877 46 TATACTA 15 0.008287406 50.154877 44 CAGAACC 15 0.008346287 50.065235 13 AGAACCT 15 0.008346287 50.065235 14 TCGGACG 20 4.1229825E-4 50.065235 14 TCGTGGC 15 0.008346287 50.065235 15 CTCGGAC 20 4.1229825E-4 50.065235 13 GAGCATA 25 2.0568961E-5 50.02054 9 CTGCTCG 20 4.1412254E-4 50.020535 10 CGATTAA 15 0.008375843 50.020535 49 ACGATTA 15 0.008375843 50.020535 48 GATGGTT 15 0.008375843 50.020535 9 ACTAATC 20 4.1412254E-4 50.020535 10 GACGCAC 15 0.008375843 50.020535 9 GATTCCC 15 0.008375843 50.020535 9 GGTTAGT 15 0.008375843 50.020535 49 >>END_MODULE