FastQCFastQC Report
Fri 17 Jun 2016
SRR1526077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11972
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC590.4928165720013365No Hit
CGCTTGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC330.27564316739057804No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC220.18376211159371866No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG210.17540928833945874No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC190.15870364183093885No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG190.15870364183093885No Hit
CGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA140.11693952555963917No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTCAGAGC140.11693952555963917No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT130.1085867023053792No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG130.1085867023053792No Hit
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10023387905111927No Hit
CGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10023387905111927No Hit
CGCTTGGGGGGGCTGGTGCGATGGCTCAGTGGGTCAGAGC120.10023387905111927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGGG5300.033.9707154
TGGGGAG850.033.9707155
CTTGGGT251.9206623E-433.970713
CTTGGGG11100.033.970713
TGGGGTA251.9206623E-433.970715
TTGGGGT700.033.970714
TGGGGGT601.8189894E-1233.970715
TGGGGGC750.033.970715
TGGGGGA1400.033.970715
TGGGGAC800.033.970715
TGGGGAA1200.033.970715
GGGGGAC251.9206623E-433.970716
GGGATCA200.002691874333.970717
TAGAGCC200.002691874333.9707134
GGGAAGC407.043491E-833.970717
GGGGAGG251.9206623E-433.970716
GGGGACA200.002691874333.970716
CGCTTGG11950.033.8285751
GCTTGGG11800.033.8267672
TGGGGGG2600.033.317435
Produced by FastQC (version 0.11.5)