FastQCFastQC Report
Fri 17 Jun 2016
SRR1526076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT40.24554941682013504No Hit
CGCTTGGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT40.24554941682013504No Hit
CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.1841620626151013No Hit
CGCTTGGGGATGGTCACCAGTCGATTTTTTTTTTTTTTTT30.1841620626151013No Hit
CGCTTGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1841620626151013No Hit
CGCTTGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.1841620626151013No Hit
CGCTTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1841620626151013No Hit
CGCTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.1841620626151013No Hit
CGCTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT30.1841620626151013No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG20.12277470841006752No Hit
CGCTTGGGGGGGCTGGTGCGATGGCCCCGTGGGTAAGAGC20.12277470841006752No Hit
CGCTTGGGGACCAACGATTTTTTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG20.12277470841006752No Hit
CGCTTGGGGTTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGGGTATGAAGGTGGAGTATTCTTCCTTGACATT20.12277470841006752No Hit
CGCTTGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC20.12277470841006752No Hit
CGCTTGGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTC20.12277470841006752No Hit
CGCTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.12277470841006752No Hit
CGCTTGGGGATGTGGTGTCTCTGTGCTAGAGCGTTTCTGT20.12277470841006752No Hit
CGCTTGGGGGTTTGTGCTTTGGATGTTTTTATAAAGTATA20.12277470841006752No Hit
CGCTTGGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC20.12277470841006752No Hit
CGCTTGGGTTCCGAGCTCGTTTTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGCTCGAAGTCGCTTTTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGGTCCTTTTTTTCTTTTGTTTTTTTGTTTTTTT20.12277470841006752No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT20.12277470841006752No Hit
CGCTTGGGGGGGGTTGGGCATTTAGCTCAGTGGTCGAGCC20.12277470841006752No Hit
CGCTTGGGGTGGAAAGTCGACTTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGGTCCGCAGAGTCCCTTTTTTTTTTTTTTTTTT20.12277470841006752No Hit
CGCTTGGGGTCCCTGGTTGATCCTGCCCGTACCCTCTGCT20.12277470841006752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGGT200.00211545433.754
TTGGGGG600.033.754
TGGGGGG200.00211545433.755
TGGGGGC251.3184611E-433.755
TGGGGCC200.00211545433.755
GGGGGCT200.00211545433.756
GGGGCCG200.00211545433.756
CGCTTGG1650.032.7272721
GCTTGGG1650.031.7045462
CTTGGGG1550.031.572583
TTGGGGA401.3332319E-629.531254
TTGGGGC352.2990718E-528.9285724
Produced by FastQC (version 0.11.5)