##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526076_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1629 Sequences flagged as poor quality 0 Sequence length 40 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 10.733578882750153 4.0 4.0 16.0 4.0 30.0 2 5.6697360343769185 4.0 4.0 4.0 4.0 9.0 3 6.411295273173726 4.0 4.0 4.0 4.0 15.0 4 5.473910374462861 4.0 4.0 4.0 4.0 4.0 5 5.761817065684469 4.0 4.0 4.0 4.0 4.0 6 4.7888275015346835 4.0 4.0 4.0 4.0 4.0 7 4.964395334561081 4.0 4.0 4.0 4.0 4.0 8 4.930632289748312 4.0 4.0 4.0 4.0 4.0 9 4.821976672805402 4.0 4.0 4.0 4.0 4.0 10 4.704726826273788 4.0 4.0 4.0 4.0 4.0 11 5.025782688766114 4.0 4.0 4.0 4.0 4.0 12 5.100675260896256 4.0 4.0 4.0 4.0 4.0 13 5.224677716390423 4.0 4.0 4.0 4.0 4.0 14 4.987108655616943 4.0 4.0 4.0 4.0 4.0 15 5.083486801718846 4.0 4.0 4.0 4.0 4.0 16 4.832412523020258 4.0 4.0 4.0 4.0 4.0 17 4.805402087170043 4.0 4.0 4.0 4.0 4.0 18 4.841006752608963 4.0 4.0 4.0 4.0 4.0 19 4.742786985880908 4.0 4.0 4.0 4.0 4.0 20 4.7612031921424185 4.0 4.0 4.0 4.0 4.0 21 4.961325966850829 4.0 4.0 4.0 4.0 4.0 22 4.969306322897483 4.0 4.0 4.0 4.0 4.0 23 4.896255371393493 4.0 4.0 4.0 4.0 4.0 24 4.862492326580725 4.0 4.0 4.0 4.0 4.0 25 4.868017188459177 4.0 4.0 4.0 4.0 4.0 26 5.0798035604665435 4.0 4.0 4.0 4.0 4.0 27 5.069981583793738 4.0 4.0 4.0 4.0 4.0 28 5.029465930018416 4.0 4.0 4.0 4.0 4.0 29 5.03621853898097 4.0 4.0 4.0 4.0 4.0 30 5.083486801718846 4.0 4.0 4.0 4.0 4.0 31 5.097605893186004 4.0 4.0 4.0 4.0 4.0 32 5.088397790055248 4.0 4.0 4.0 4.0 4.0 33 5.095150399017802 4.0 4.0 4.0 4.0 4.0 34 5.120319214241866 4.0 4.0 4.0 4.0 4.0 35 5.141804788213628 4.0 4.0 4.0 4.0 4.0 36 4.925721301411909 4.0 4.0 4.0 4.0 4.0 37 4.857581338244322 4.0 4.0 4.0 4.0 4.0 38 4.878453038674033 4.0 4.0 4.0 4.0 4.0 39 4.858195211786372 4.0 4.0 4.0 4.0 4.0 40 4.849600982197667 4.0 4.0 4.0 4.0 4.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 25.552721088435376 1 2 21.714285714285715 1 3 17.7015306122449 1 4 19.11904761904762 1 5 21.882653061224488 1 6 -1.0850340136054424 1 7 -1.4404761904761898 1 8 -0.9438775510204085 1 9 -0.9166666666666661 1 10 -0.8877551020408161 1 11 -1.1921768707482991 1 12 -1.0476190476190474 1 13 -1.1700680272108839 1 14 -0.8316326530612246 1 15 -1.1190476190476186 1 16 -0.77891156462585 1 17 -0.732993197278911 1 18 -1.1003401360544212 1 19 -0.4574829931972788 1 20 -0.6819727891156457 1 21 -0.5255102040816331 1 22 -0.4931972789115644 1 23 -0.44217687074829914 1 24 -0.5187074829931975 1 25 -0.49829931972789065 1 26 -0.7193877551020407 1 27 -0.5901360544217686 1 28 -0.6360544217687076 1 29 -0.6309523809523805 1 30 -0.6156462585034008 1 31 -0.5952380952380949 1 32 -0.6462585034013602 1 33 -0.6054421768707483 1 34 -0.5850340136054424 1 35 -0.6683673469387754 1 36 -0.4370748299319729 1 37 -0.3979591836734695 1 38 -0.44217687074829914 1 39 -0.4166666666666661 1 40 -0.44217687074829914 2 1 7.552721088435375 2 2 -1.2857142857142856 2 3 -2.048469387755101 2 4 -1.3809523809523805 2 5 -1.6173469387755102 2 6 -1.0850340136054424 2 7 -1.4404761904761898 2 8 -0.9438775510204085 2 9 -0.9166666666666661 2 10 -0.8877551020408161 2 11 -1.1921768707482991 2 12 -1.0476190476190474 2 13 -1.1700680272108839 2 14 -0.8316326530612246 2 15 -1.1190476190476186 2 16 -0.77891156462585 2 17 -0.732993197278911 2 18 -1.1003401360544212 2 19 -0.4574829931972788 2 20 -0.6819727891156457 2 21 -0.5255102040816331 2 22 -0.4931972789115644 2 23 -0.44217687074829914 2 24 -0.5187074829931975 2 25 -0.49829931972789065 2 26 -0.7193877551020407 2 27 -0.5901360544217686 2 28 -0.6360544217687076 2 29 -0.6309523809523805 2 30 -0.6156462585034008 2 31 -0.5952380952380949 2 32 -0.6462585034013602 2 33 -0.6054421768707483 2 34 -0.5850340136054424 2 35 -0.6683673469387754 2 36 -0.4370748299319729 2 37 -0.3979591836734695 2 38 -0.44217687074829914 2 39 -0.4166666666666661 2 40 -0.44217687074829914 3 1 -5.447278911564625 3 2 -1.2857142857142856 3 3 -2.048469387755101 3 4 -1.3809523809523805 3 5 -1.6173469387755102 3 6 -1.0850340136054424 3 7 -1.4404761904761898 3 8 -0.9438775510204085 3 9 -0.9166666666666661 3 10 -0.8877551020408161 3 11 -1.1921768707482991 3 12 -1.0476190476190474 3 13 -1.1700680272108839 3 14 -0.8316326530612246 3 15 -1.1190476190476186 3 16 -0.77891156462585 3 17 -0.732993197278911 3 18 -1.1003401360544212 3 19 -0.4574829931972788 3 20 -0.6819727891156457 3 21 -0.5255102040816331 3 22 -0.4931972789115644 3 23 -0.44217687074829914 3 24 -0.5187074829931975 3 25 -0.49829931972789065 3 26 -0.7193877551020407 3 27 -0.5901360544217686 3 28 -0.6360544217687076 3 29 -0.6309523809523805 3 30 -0.6156462585034008 3 31 -0.5952380952380949 3 32 -0.6462585034013602 3 33 -0.6054421768707483 3 34 -0.5850340136054424 3 35 -0.6683673469387754 3 36 -0.4370748299319729 3 37 -0.3979591836734695 3 38 -0.44217687074829914 3 39 -0.4166666666666661 3 40 -0.44217687074829914 6 1 -5.447278911564625 6 2 -1.2857142857142856 6 3 -2.048469387755101 6 4 -1.3809523809523805 6 5 -1.6173469387755102 6 6 -1.0850340136054424 6 7 -1.4404761904761898 6 8 -0.9438775510204085 6 9 -0.9166666666666661 6 10 -0.8877551020408161 6 11 -1.1921768707482991 6 12 -1.0476190476190474 6 13 -1.1700680272108839 6 14 -0.8316326530612246 6 15 -1.1190476190476186 6 16 -0.77891156462585 6 17 -0.732993197278911 6 18 -1.1003401360544212 6 19 -0.4574829931972788 6 20 -0.6819727891156457 6 21 -0.5255102040816331 6 22 -0.4931972789115644 6 23 -0.44217687074829914 6 24 -0.5187074829931975 6 25 -0.49829931972789065 6 26 -0.7193877551020407 6 27 -0.5901360544217686 6 28 -0.6360544217687076 6 29 -0.6309523809523805 6 30 -0.6156462585034008 6 31 -0.5952380952380949 6 32 -0.6462585034013602 6 33 -0.6054421768707483 6 34 -0.5850340136054424 6 35 -0.6683673469387754 6 36 -0.4370748299319729 6 37 -0.3979591836734695 6 38 -0.44217687074829914 6 39 -0.4166666666666661 6 40 -0.44217687074829914 8 1 20.552721088435376 8 2 -1.2857142857142856 8 3 -2.048469387755101 8 4 -1.3809523809523805 8 5 -1.6173469387755102 8 6 -1.0850340136054424 8 7 -1.4404761904761898 8 8 -0.9438775510204085 8 9 -0.9166666666666661 8 10 -0.8877551020408161 8 11 -1.1921768707482991 8 12 -1.0476190476190474 8 13 -1.1700680272108839 8 14 -0.8316326530612246 8 15 -1.1190476190476186 8 16 -0.77891156462585 8 17 -0.732993197278911 8 18 -1.1003401360544212 8 19 -0.4574829931972788 8 20 -0.6819727891156457 8 21 -0.5255102040816331 8 22 -0.4931972789115644 8 23 -0.44217687074829914 8 24 -0.5187074829931975 8 25 -0.49829931972789065 8 26 -0.7193877551020407 8 27 -0.5901360544217686 8 28 -0.6360544217687076 8 29 -0.6309523809523805 8 30 -0.6156462585034008 8 31 -0.5952380952380949 8 32 -0.6462585034013602 8 33 -0.6054421768707483 8 34 -0.5850340136054424 8 35 -0.6683673469387754 8 36 -0.4370748299319729 8 37 -0.3979591836734695 8 38 -0.44217687074829914 8 39 -0.4166666666666661 8 40 -0.44217687074829914 9 1 8.552721088435375 9 2 1.7142857142857144 9 3 11.951530612244898 9 4 -1.3809523809523805 9 5 -1.6173469387755102 9 6 -1.0850340136054424 9 7 -1.4404761904761898 9 8 -0.9438775510204085 9 9 -0.9166666666666661 9 10 -0.8877551020408161 9 11 -1.1921768707482991 9 12 -1.0476190476190474 9 13 -1.1700680272108839 9 14 -0.8316326530612246 9 15 -1.1190476190476186 9 16 -0.77891156462585 9 17 -0.732993197278911 9 18 -1.1003401360544212 9 19 -0.4574829931972788 9 20 -0.6819727891156457 9 21 -0.5255102040816331 9 22 -0.4931972789115644 9 23 -0.44217687074829914 9 24 -0.5187074829931975 9 25 -0.49829931972789065 9 26 -0.7193877551020407 9 27 -0.5901360544217686 9 28 -0.6360544217687076 9 29 -0.6309523809523805 9 30 -0.6156462585034008 9 31 -0.5952380952380949 9 32 -0.6462585034013602 9 33 -0.6054421768707483 9 34 -0.5850340136054424 9 35 -0.6683673469387754 9 36 -0.4370748299319729 9 37 -0.3979591836734695 9 38 -0.44217687074829914 9 39 -0.4166666666666661 9 40 -0.44217687074829914 10 1 -5.447278911564625 10 2 -1.2857142857142856 10 3 -2.048469387755101 10 4 -1.3809523809523805 10 5 -1.6173469387755102 10 6 -1.0850340136054424 10 7 -1.4404761904761898 10 8 -0.9438775510204085 10 9 -0.9166666666666661 10 10 -0.8877551020408161 10 11 -1.1921768707482991 10 12 -1.0476190476190474 10 13 -1.1700680272108839 10 14 -0.8316326530612246 10 15 -1.1190476190476186 10 16 -0.77891156462585 10 17 -0.732993197278911 10 18 -1.1003401360544212 10 19 -0.4574829931972788 10 20 -0.6819727891156457 10 21 -0.5255102040816331 10 22 -0.4931972789115644 10 23 -0.44217687074829914 10 24 -0.5187074829931975 10 25 -0.49829931972789065 10 26 -0.7193877551020407 10 27 -0.5901360544217686 10 28 -0.6360544217687076 10 29 -0.6309523809523805 10 30 -0.6156462585034008 10 31 -0.5952380952380949 10 32 -0.6462585034013602 10 33 -0.6054421768707483 10 34 -0.5850340136054424 10 35 -0.6683673469387754 10 36 -0.4370748299319729 10 37 -0.3979591836734695 10 38 -0.44217687074829914 10 39 -0.4166666666666661 10 40 -0.44217687074829914 11 1 -5.447278911564625 11 2 -1.2857142857142856 11 3 -2.048469387755101 11 4 -1.3809523809523805 11 5 -1.6173469387755102 11 6 -1.0850340136054424 11 7 -1.4404761904761898 11 8 -0.9438775510204085 11 9 -0.9166666666666661 11 10 -0.8877551020408161 11 11 -1.1921768707482991 11 12 -1.0476190476190474 11 13 -1.1700680272108839 11 14 -0.8316326530612246 11 15 -1.1190476190476186 11 16 -0.77891156462585 11 17 -0.732993197278911 11 18 -1.1003401360544212 11 19 -0.4574829931972788 11 20 -0.6819727891156457 11 21 -0.5255102040816331 11 22 -0.4931972789115644 11 23 -0.44217687074829914 11 24 -0.5187074829931975 11 25 -0.49829931972789065 11 26 -0.7193877551020407 11 27 -0.5901360544217686 11 28 -0.6360544217687076 11 29 -0.6309523809523805 11 30 -0.6156462585034008 11 31 -0.5952380952380949 11 32 -0.6462585034013602 11 33 -0.6054421768707483 11 34 -0.5850340136054424 11 35 -0.6683673469387754 11 36 -0.4370748299319729 11 37 -0.3979591836734695 11 38 -0.44217687074829914 11 39 -0.4166666666666661 11 40 -0.44217687074829914 12 1 7.052721088435375 12 2 -0.2857142857142856 12 3 9.451530612244898 12 4 3.6190476190476195 12 5 2.38265306122449 12 6 1.9149659863945576 12 7 3.05952380952381 12 8 2.5561224489795915 12 9 1.083333333333334 12 10 1.612244897959184 12 11 8.307823129251702 12 12 1.9523809523809526 12 13 0.3299319727891161 12 14 0.16836734693877542 12 15 -0.11904761904761862 12 16 1.72108843537415 12 17 1.767006802721089 12 18 8.39965986394558 12 19 2.542517006802721 12 20 3.3180272108843543 12 21 4.474489795918367 12 22 3.5068027210884356 12 23 7.557823129251701 12 24 4.9812925170068025 12 25 5.001700680272109 12 26 14.28061224489796 12 27 13.40986394557823 12 28 13.363945578231291 12 29 14.36904761904762 12 30 13.3843537414966 12 31 13.404761904761905 12 32 13.35374149659864 12 33 13.39455782312925 12 34 13.414965986394558 12 35 13.331632653061224 12 36 7.562925170068027 12 37 5.1020408163265305 12 38 10.557823129251702 12 39 8.583333333333334 12 40 7.557823129251701 13 1 -5.447278911564625 13 2 -1.2857142857142856 13 3 -2.048469387755101 13 4 -1.3809523809523805 13 5 -1.6173469387755102 13 6 -1.0850340136054424 13 7 -1.4404761904761898 13 8 -0.9438775510204085 13 9 -0.9166666666666661 13 10 -0.8877551020408161 13 11 -1.1921768707482991 13 12 -1.0476190476190474 13 13 -1.1700680272108839 13 14 -0.8316326530612246 13 15 -1.1190476190476186 13 16 -0.77891156462585 13 17 -0.732993197278911 13 18 -1.1003401360544212 13 19 -0.4574829931972788 13 20 -0.6819727891156457 13 21 -0.5255102040816331 13 22 -0.4931972789115644 13 23 -0.44217687074829914 13 24 -0.5187074829931975 13 25 -0.49829931972789065 13 26 -0.7193877551020407 13 27 -0.5901360544217686 13 28 -0.6360544217687076 13 29 -0.6309523809523805 13 30 -0.6156462585034008 13 31 -0.5952380952380949 13 32 -0.6462585034013602 13 33 -0.6054421768707483 13 34 -0.5850340136054424 13 35 -0.6683673469387754 13 36 -0.4370748299319729 13 37 -0.3979591836734695 13 38 -0.44217687074829914 13 39 -0.4166666666666661 13 40 -0.44217687074829914 14 1 -5.447278911564625 14 2 -1.2857142857142856 14 3 -2.048469387755101 14 4 -1.3809523809523805 14 5 -1.6173469387755102 14 6 -1.0850340136054424 14 7 -1.4404761904761898 14 8 -0.9438775510204085 14 9 -0.9166666666666661 14 10 -0.8877551020408161 14 11 -1.1921768707482991 14 12 -1.0476190476190474 14 13 -1.1700680272108839 14 14 -0.8316326530612246 14 15 -1.1190476190476186 14 16 -0.77891156462585 14 17 -0.732993197278911 14 18 -1.1003401360544212 14 19 -0.4574829931972788 14 20 -0.6819727891156457 14 21 -0.5255102040816331 14 22 -0.4931972789115644 14 23 -0.44217687074829914 14 24 -0.5187074829931975 14 25 -0.49829931972789065 14 26 -0.7193877551020407 14 27 -0.5901360544217686 14 28 -0.6360544217687076 14 29 -0.6309523809523805 14 30 -0.6156462585034008 14 31 -0.5952380952380949 14 32 -0.6462585034013602 14 33 -0.6054421768707483 14 34 -0.5850340136054424 14 35 -0.6683673469387754 14 36 -0.4370748299319729 14 37 -0.3979591836734695 14 38 -0.44217687074829914 14 39 -0.4166666666666661 14 40 -0.44217687074829914 15 1 -5.447278911564625 15 2 -1.2857142857142856 15 3 -2.048469387755101 15 4 -1.3809523809523805 15 5 -1.6173469387755102 15 6 -1.0850340136054424 15 7 -1.4404761904761898 15 8 -0.9438775510204085 15 9 -0.9166666666666661 15 10 -0.8877551020408161 15 11 -1.1921768707482991 15 12 -1.0476190476190474 15 13 -1.1700680272108839 15 14 -0.8316326530612246 15 15 -1.1190476190476186 15 16 -0.77891156462585 15 17 -0.732993197278911 15 18 -1.1003401360544212 15 19 -0.4574829931972788 15 20 -0.6819727891156457 15 21 -0.5255102040816331 15 22 -0.4931972789115644 15 23 -0.44217687074829914 15 24 -0.5187074829931975 15 25 -0.49829931972789065 15 26 -0.7193877551020407 15 27 -0.5901360544217686 15 28 -0.6360544217687076 15 29 -0.6309523809523805 15 30 -0.6156462585034008 15 31 -0.5952380952380949 15 32 -0.6462585034013602 15 33 -0.6054421768707483 15 34 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-0.3979591836734695 111 38 -0.44217687074829914 111 39 -0.4166666666666661 111 40 -0.44217687074829914 112 1 -5.447278911564625 112 2 -1.2857142857142856 112 3 -2.048469387755101 112 4 -1.3809523809523805 112 5 -1.6173469387755102 112 6 -1.0850340136054424 112 7 -1.4404761904761898 112 8 -0.9438775510204085 112 9 -0.9166666666666661 112 10 -0.8877551020408161 112 11 -1.1921768707482991 112 12 -1.0476190476190474 112 13 -1.1700680272108839 112 14 -0.8316326530612246 112 15 -1.1190476190476186 112 16 -0.77891156462585 112 17 -0.732993197278911 112 18 -1.1003401360544212 112 19 -0.4574829931972788 112 20 -0.6819727891156457 112 21 -0.5255102040816331 112 22 -0.4931972789115644 112 23 -0.44217687074829914 112 24 -0.5187074829931975 112 25 -0.49829931972789065 112 26 -0.7193877551020407 112 27 -0.5901360544217686 112 28 -0.6360544217687076 112 29 -0.6309523809523805 112 30 -0.6156462585034008 112 31 -0.5952380952380949 112 32 -0.6462585034013602 112 33 -0.6054421768707483 112 34 -0.5850340136054424 112 35 -0.6683673469387754 112 36 -0.4370748299319729 112 37 -0.3979591836734695 112 38 -0.44217687074829914 112 39 -0.4166666666666661 112 40 -0.44217687074829914 113 1 -5.447278911564625 113 2 -1.2857142857142856 113 3 -2.048469387755101 113 4 -1.3809523809523805 113 5 -1.6173469387755102 113 6 -1.0850340136054424 113 7 -1.4404761904761898 113 8 -0.9438775510204085 113 9 -0.9166666666666661 113 10 -0.8877551020408161 113 11 -1.1921768707482991 113 12 -1.0476190476190474 113 13 -1.1700680272108839 113 14 -0.8316326530612246 113 15 -1.1190476190476186 113 16 -0.77891156462585 113 17 -0.732993197278911 113 18 -1.1003401360544212 113 19 -0.4574829931972788 113 20 -0.6819727891156457 113 21 -0.5255102040816331 113 22 -0.4931972789115644 113 23 -0.44217687074829914 113 24 -0.5187074829931975 113 25 -0.49829931972789065 113 26 -0.7193877551020407 113 27 -0.5901360544217686 113 28 -0.6360544217687076 113 29 -0.6309523809523805 113 30 -0.6156462585034008 113 31 -0.5952380952380949 113 32 -0.6462585034013602 113 33 -0.6054421768707483 113 34 -0.5850340136054424 113 35 -0.6683673469387754 113 36 -0.4370748299319729 113 37 -0.3979591836734695 113 38 -0.44217687074829914 113 39 -0.4166666666666661 113 40 -0.44217687074829914 114 1 -5.447278911564625 114 2 -1.2857142857142856 114 3 -2.048469387755101 114 4 -1.3809523809523805 114 5 -1.6173469387755102 114 6 -1.0850340136054424 114 7 -1.4404761904761898 114 8 -0.9438775510204085 114 9 -0.9166666666666661 114 10 -0.8877551020408161 114 11 -1.1921768707482991 114 12 -1.0476190476190474 114 13 -1.1700680272108839 114 14 -0.8316326530612246 114 15 -1.1190476190476186 114 16 -0.77891156462585 114 17 -0.732993197278911 114 18 -1.1003401360544212 114 19 -0.4574829931972788 114 20 -0.6819727891156457 114 21 -0.5255102040816331 114 22 -0.4931972789115644 114 23 -0.44217687074829914 114 24 -0.5187074829931975 114 25 -0.49829931972789065 114 26 -0.7193877551020407 114 27 -0.5901360544217686 114 28 -0.6360544217687076 114 29 -0.6309523809523805 114 30 -0.6156462585034008 114 31 -0.5952380952380949 114 32 -0.6462585034013602 114 33 -0.6054421768707483 114 34 -0.5850340136054424 114 35 -0.6683673469387754 114 36 -0.4370748299319729 114 37 -0.3979591836734695 114 38 -0.44217687074829914 114 39 -0.4166666666666661 114 40 -0.44217687074829914 116 1 18.552721088435376 116 2 6.714285714285714 116 3 8.951530612244898 116 4 19.61904761904762 116 5 13.38265306122449 116 6 27.914965986394556 116 7 27.55952380952381 116 8 -0.9438775510204085 116 9 -0.9166666666666661 116 10 -0.8877551020408161 116 11 -1.1921768707482991 116 12 -1.0476190476190474 116 13 -1.1700680272108839 116 14 -0.8316326530612246 116 15 -1.1190476190476186 116 16 -0.77891156462585 116 17 -0.732993197278911 116 18 -1.1003401360544212 116 19 -0.4574829931972788 116 20 -0.6819727891156457 116 21 -0.5255102040816331 116 22 -0.4931972789115644 116 23 -0.44217687074829914 116 24 -0.5187074829931975 116 25 -0.49829931972789065 116 26 -0.7193877551020407 116 27 -0.5901360544217686 116 28 -0.6360544217687076 116 29 -0.6309523809523805 116 30 -0.6156462585034008 116 31 -0.5952380952380949 116 32 -0.6462585034013602 116 33 -0.6054421768707483 116 34 -0.5850340136054424 116 35 -0.6683673469387754 116 36 -0.4370748299319729 116 37 -0.3979591836734695 116 38 -0.44217687074829914 116 39 -0.4166666666666661 116 40 -0.44217687074829914 118 1 -5.447278911564625 118 2 -1.2857142857142856 118 3 -2.048469387755101 118 4 -1.3809523809523805 118 5 -1.6173469387755102 118 6 -1.0850340136054424 118 7 -1.4404761904761898 118 8 -0.9438775510204085 118 9 -0.9166666666666661 118 10 -0.8877551020408161 118 11 -1.1921768707482991 118 12 -1.0476190476190474 118 13 -1.1700680272108839 118 14 -0.8316326530612246 118 15 -1.1190476190476186 118 16 -0.77891156462585 118 17 -0.732993197278911 118 18 -1.1003401360544212 118 19 -0.4574829931972788 118 20 -0.6819727891156457 118 21 -0.5255102040816331 118 22 -0.4931972789115644 118 23 -0.44217687074829914 118 24 -0.5187074829931975 118 25 -0.49829931972789065 118 26 -0.7193877551020407 118 27 -0.5901360544217686 118 28 -0.6360544217687076 118 29 -0.6309523809523805 118 30 -0.6156462585034008 118 31 -0.5952380952380949 118 32 -0.6462585034013602 118 33 -0.6054421768707483 118 34 -0.5850340136054424 118 35 -0.6683673469387754 118 36 -0.4370748299319729 118 37 -0.3979591836734695 118 38 -0.44217687074829914 118 39 -0.4166666666666661 118 40 -0.44217687074829914 120 1 -5.447278911564625 120 2 -1.2857142857142856 120 3 -2.048469387755101 120 4 -1.3809523809523805 120 5 -1.6173469387755102 120 6 -1.0850340136054424 120 7 -1.4404761904761898 120 8 -0.9438775510204085 120 9 -0.9166666666666661 120 10 -0.8877551020408161 120 11 -1.1921768707482991 120 12 -1.0476190476190474 120 13 -1.1700680272108839 120 14 -0.8316326530612246 120 15 -1.1190476190476186 120 16 -0.77891156462585 120 17 -0.732993197278911 120 18 -1.1003401360544212 120 19 -0.4574829931972788 120 20 -0.6819727891156457 120 21 -0.5255102040816331 120 22 -0.4931972789115644 120 23 -0.44217687074829914 120 24 -0.5187074829931975 120 25 -0.49829931972789065 120 26 -0.7193877551020407 120 27 -0.5901360544217686 120 28 -0.6360544217687076 120 29 -0.6309523809523805 120 30 -0.6156462585034008 120 31 -0.5952380952380949 120 32 -0.6462585034013602 120 33 -0.6054421768707483 120 34 -0.5850340136054424 120 35 -0.6683673469387754 120 36 -0.4370748299319729 120 37 -0.3979591836734695 120 38 -0.44217687074829914 120 39 -0.4166666666666661 120 40 -0.44217687074829914 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1439.0 5 29.0 6 9.0 7 30.0 8 8.0 9 5.0 10 12.0 11 4.0 12 5.0 13 7.0 14 8.0 15 1.0 16 3.0 17 1.0 18 6.0 19 14.0 20 12.0 21 11.0 22 9.0 23 8.0 24 6.0 25 1.0 26 0.0 27 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 98.76695437731196 0.3082614056720099 0.2466091245376079 0.6781750924784217 8 98.46531614487417 0.6138735420503376 0.42971147943523635 0.49109883364027007 9 89.31860036832413 4.112952731737262 3.376304481276857 3.192142418661756 10 32.96500920810313 28.790669122160832 14.732965009208105 23.511356660527934 11 29.46593001841621 24.30939226519337 19.03007980356047 27.19459791282996 12 37.93738489871086 11.540822590546346 22.65193370165746 27.86985880908533 13 24.861878453038674 16.390423572744016 27.56292203806016 31.18477593615715 14 21.976672805402085 20.012277470841006 31.675874769797424 26.335174953959484 15 29.097605893186003 14.794352363413138 29.404542664211174 26.703499079189687 16 28.852056476365867 13.750767341927563 29.158993247391034 28.23818293431553 17 22.774708410067525 17.249846531614487 29.711479435236342 30.263965623081646 18 20.012277470841006 20.196439533456108 28.360957642725598 31.430325352977285 19 18.165024630541872 17.733990147783253 25.923645320197043 38.17733990147783 20 21.117249846531614 21.05586249232658 26.887661141804784 30.939226519337016 21 19.0914671577655 17.80233271945979 31.860036832412526 31.246163290362183 22 16.881522406384285 18.96869244935543 28.299570288520563 35.850214855739715 23 20.626151012891345 16.32903621853898 28.360957642725598 34.683855125844076 24 17.61817065684469 21.976672805402085 32.96500920810313 27.440147329650095 25 21.79802955665025 14.408866995073891 29.125615763546797 34.66748768472907 26 16.666666666666664 16.051660516605164 32.10332103321033 35.17835178351783 27 18.974042027194066 16.625463535228675 33.25092707045735 31.1495673671199 28 15.682656826568268 18.019680196801968 33.886838868388686 32.41082410824108 29 17.23076923076923 17.046153846153846 36.92307692307693 28.799999999999997 30 12.829957028852057 16.881522406384285 42.7255985267035 27.56292203806016 31 13.882063882063884 15.11056511056511 42.62899262899263 28.37837837837838 32 15.715162676488642 14.119091467157766 41.92756292203806 28.23818293431553 33 13.19828115408226 11.90914671577655 43.278084714548804 31.61448741559239 34 10.92694904849601 13.382443216697359 49.72375690607735 25.96685082872928 35 9.6378146101903 14.794352363413138 49.53959484346225 26.028238182934317 36 13.144963144963146 17.32186732186732 46.253071253071255 23.28009828009828 37 9.667487684729064 15.39408866995074 50.98522167487685 23.95320197044335 38 12.584407612031923 15.039901780233272 47.94352363413137 24.432166973603437 39 11.11111111111111 14.180478821362799 47.20687538367096 27.501534683855127 40 9.4536525475752 15.285451197053407 48.12768569674647 27.13321055862492 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 2.0 19 3.5 20 5.0 21 8.5 22 12.0 23 12.0 24 19.0 25 26.0 26 31.5 27 37.0 28 37.0 29 40.5 30 44.0 31 41.5 32 39.0 33 39.0 34 34.5 35 30.0 36 44.0 37 58.0 38 58.0 39 56.5 40 55.0 41 62.5 42 70.0 43 70.0 44 68.5 45 67.0 46 77.0 47 87.0 48 87.0 49 90.5 50 94.0 51 97.5 52 101.0 53 101.0 54 106.5 55 112.0 56 113.0 57 114.0 58 114.0 59 110.0 60 106.0 61 111.5 62 117.0 63 117.0 64 103.0 65 89.0 66 91.0 67 93.0 68 93.0 69 90.5 70 88.0 71 79.5 72 71.0 73 71.0 74 56.0 75 41.0 76 34.0 77 27.0 78 27.0 79 22.5 80 18.0 81 14.0 82 10.0 83 10.0 84 8.0 85 6.0 86 5.5 87 5.0 88 5.0 89 3.5 90 2.0 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.42971147943523635 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.3069367710251688 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.3069367710251688 26 0.1841620626151013 27 0.6752608962553714 28 0.1841620626151013 29 0.24554941682013504 30 0.0 31 0.06138735420503376 32 0.0 33 0.0 34 0.0 35 0.0 36 0.06138735420503376 37 0.3069367710251688 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1629.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.42173112338858 #Duplication Level Percentage of deduplicated Percentage of total 1 98.04662885948329 95.51872314303253 2 1.3862633900441084 2.701043585021486 3 0.4410838059231254 1.289134438305709 4 0.1260239445494644 0.49109883364027007 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCTTGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT 4 0.24554941682013504 No Hit CGCTTGGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 4 0.24554941682013504 No Hit CGCTTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.1841620626151013 No Hit CGCTTGGGGATGGTCACCAGTCGATTTTTTTTTTTTTTTT 3 0.1841620626151013 No Hit CGCTTGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.1841620626151013 No Hit CGCTTGGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.1841620626151013 No Hit CGCTTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.1841620626151013 No Hit CGCTTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.1841620626151013 No Hit CGCTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 3 0.1841620626151013 No Hit CGCTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG 2 0.12277470841006752 No Hit CGCTTGGGGGGGCTGGTGCGATGGCCCCGTGGGTAAGAGC 2 0.12277470841006752 No Hit CGCTTGGGGACCAACGATTTTTTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG 2 0.12277470841006752 No Hit CGCTTGGGGTTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGGTATGAAGGTGGAGTATTCTTCCTTGACATT 2 0.12277470841006752 No Hit CGCTTGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC 2 0.12277470841006752 No Hit CGCTTGGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTC 2 0.12277470841006752 No Hit CGCTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.12277470841006752 No Hit CGCTTGGGGATGTGGTGTCTCTGTGCTAGAGCGTTTCTGT 2 0.12277470841006752 No Hit CGCTTGGGGGTTTGTGCTTTGGATGTTTTTATAAAGTATA 2 0.12277470841006752 No Hit CGCTTGGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC 2 0.12277470841006752 No Hit CGCTTGGGTTCCGAGCTCGTTTTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGCTCGAAGTCGCTTTTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGTCCTTTTTTTCTTTTGTTTTTTTGTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 2 0.12277470841006752 No Hit CGCTTGGGGGGGGTTGGGCATTTAGCTCAGTGGTCGAGCC 2 0.12277470841006752 No Hit CGCTTGGGGTGGAAAGTCGACTTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGTCCGCAGAGTCCCTTTTTTTTTTTTTTTTTT 2 0.12277470841006752 No Hit CGCTTGGGGTCCCTGGTTGATCCTGCCCGTACCCTCTGCT 2 0.12277470841006752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGGGT 20 0.002115454 33.75 4 TTGGGGG 60 0.0 33.75 4 TGGGGGG 20 0.002115454 33.75 5 TGGGGGC 25 1.3184611E-4 33.75 5 TGGGGCC 20 0.002115454 33.75 5 GGGGGCT 20 0.002115454 33.75 6 GGGGCCG 20 0.002115454 33.75 6 CGCTTGG 165 0.0 32.727272 1 GCTTGGG 165 0.0 31.704546 2 CTTGGGG 155 0.0 31.57258 3 TTGGGGA 40 1.3332319E-6 29.53125 4 TTGGGGC 35 2.2990718E-5 28.928572 4 >>END_MODULE