FastQCFastQC Report
Fri 17 Jun 2016
SRR1526075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6036
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1943.214049039098741No Hit
CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT831.3750828363154408No Hit
CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA671.110006626905235No Hit
CGCTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG600.9940357852882704No Hit
CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC180.2982107355864811No Hit
CGCTTGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT160.26507620941020543No Hit
CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.2485089463220676No Hit
CGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.23194168323392977No Hit
CGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.21537442014579194No Hit
CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG110.18223989396951623No Hit
CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC100.16567263088137837No Hit
CGCTTGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT100.16567263088137837No Hit
CGCTTGGGGGTTTCTCATAGCCTCGTTGTGTGGCTTTGGC100.16567263088137837No Hit
CGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT90.14910536779324055No Hit
CGCTTGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT90.14910536779324055No Hit
CGCTTGGGGGTCATCTTCCAGGAACTGCTCACTGACAAGT90.14910536779324055No Hit
CGCTTGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT90.14910536779324055No Hit
CGCTTGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT90.14910536779324055No Hit
CGCTTGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT80.13253810470510272No Hit
CGCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG80.13253810470510272No Hit
CGCTTGGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT70.11597084161696489No Hit
CGCTTGGGGCAGACTTGTATTTACATCTCATAGATATTTT70.11597084161696489No Hit
CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.11597084161696489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC358.71325E-734.020
TGGTTGA358.71325E-734.014
GGGTCAC301.2536255E-534.07
GTCACAG251.7985818E-434.09
GTACCTG251.7985818E-434.09
CCAGTAG358.71325E-734.026
AGTAGCA301.2536255E-534.028
CCTGCCA358.71325E-734.022
CAGTAGC301.2536255E-534.027
TGATCCT358.71325E-734.018
GGTCACA251.7985818E-434.08
GCCAGTA358.71325E-734.025
CTGGTTG358.71325E-734.013
ACCTGGT251.7985818E-434.011
TGGGGTC454.1600288E-934.05
TGGGGTA251.7985818E-434.05
TGGGGGG850.034.05
TGGGGCG301.2536255E-534.05
TGGGGAT552.0008883E-1134.05
GATCCTG358.71325E-734.019
Produced by FastQC (version 0.11.5)