##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526075_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6036 Sequences flagged as poor quality 0 Sequence length 40 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.905732273028494 33.0 30.0 35.0 4.0 37.0 2 20.445162359178262 23.0 4.0 32.0 4.0 35.0 3 19.159045725646124 22.0 4.0 30.0 4.0 33.0 4 20.182405566600398 23.0 4.0 32.0 4.0 35.0 5 20.04075546719682 24.0 4.0 33.0 4.0 35.0 6 15.107190192180251 11.0 4.0 27.0 4.0 33.0 7 15.975646123260438 11.0 4.0 30.0 4.0 33.0 8 14.839960238568588 4.0 4.0 28.0 4.0 33.0 9 13.970510271703114 4.0 4.0 27.0 4.0 33.0 10 13.373591782637508 4.0 4.0 26.0 4.0 33.0 11 10.674453280318092 4.0 4.0 17.0 4.0 30.0 12 10.486414844267728 4.0 4.0 17.0 4.0 29.0 13 10.156063618290258 4.0 4.0 15.0 4.0 29.0 14 10.120609675281644 4.0 4.0 15.0 4.0 30.0 15 9.799370444002651 4.0 4.0 13.0 4.0 29.0 16 8.589960238568588 4.0 4.0 8.0 4.0 26.0 17 8.375579854208086 4.0 4.0 7.0 4.0 24.0 18 8.25364479787939 4.0 4.0 4.0 4.0 23.0 19 7.959741550695825 4.0 4.0 4.0 4.0 22.0 20 7.942511597084161 4.0 4.0 4.0 4.0 23.0 21 7.863154406891981 4.0 4.0 4.0 4.0 23.0 22 7.818588469184891 4.0 4.0 4.0 4.0 23.0 23 8.02269715043075 4.0 4.0 4.0 4.0 24.0 24 7.763419483101392 4.0 4.0 4.0 4.0 23.0 25 7.944002650762094 4.0 4.0 4.0 4.0 24.0 26 8.026010603048377 4.0 4.0 4.0 4.0 25.0 27 8.05649436713055 4.0 4.0 4.0 4.0 25.0 28 8.238568588469185 4.0 4.0 4.0 4.0 27.0 29 7.608846918489066 4.0 4.0 4.0 4.0 22.0 30 8.120444002650762 4.0 4.0 4.0 4.0 26.0 31 8.44118621603711 4.0 4.0 4.0 4.0 29.0 32 8.404075546719682 4.0 4.0 4.0 4.0 29.0 33 8.316931742876077 4.0 4.0 4.0 4.0 28.0 34 8.389662027833001 4.0 4.0 4.0 4.0 29.0 35 8.166832339297548 4.0 4.0 4.0 4.0 28.0 36 8.111994698475812 4.0 4.0 4.0 4.0 29.0 37 7.9713386348575215 4.0 4.0 4.0 4.0 29.0 38 7.8031809145129225 4.0 4.0 4.0 4.0 28.0 39 7.587143803843605 4.0 4.0 4.0 4.0 26.0 40 7.672962226640159 4.0 4.0 4.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -10.662995709647479 1 2 -15.066653460626675 1 3 -13.391117488662129 1 4 -14.850506660997734 1 5 -14.70780670222635 1 6 -10.128089891259535 1 7 -11.122049577406715 1 8 -9.380741084312508 1 9 -8.850450937950939 1 10 -8.378470611729542 1 11 -5.927061752731396 1 12 -5.765064226448155 1 13 -5.653936044114619 1 14 -5.465184369202225 1 15 -5.076129277468564 1 16 -3.6567299268192093 1 17 -3.693407609255824 1 18 -3.4106988249845385 1 19 -3.318118042671615 1 20 -3.186528228715729 1 21 -3.079412105751392 1 22 -2.9599325525664826 1 23 -3.128756635229851 1 24 -3.2325715702947857 1 25 -3.0460993867243866 1 26 -3.1708075654504224 1 27 -3.2144979772211917 1 28 -3.275054756751187 1 29 -2.6015070990517435 1 30 -3.295839775304059 1 31 -3.4936259920634924 1 32 -3.5394660894660888 1 33 -3.6134714491857354 1 34 -3.4706213924963905 1 35 -3.1593350597814895 1 36 -3.083196119356832 1 37 -3.154811830035043 1 38 -3.0386192924139346 1 39 -2.7609806612038765 1 40 -2.827174165120594 2 1 7.037004290352524 2 2 -0.0666534606266751 2 3 6.608882511337871 2 4 5.349493339002265 2 5 9.29219329777365 2 6 -2.528089891259535 2 7 -6.122049577406715 2 8 -3.580741084312507 2 9 -7.450450937950938 2 10 -0.7784706117295421 2 11 -3.7270617527313954 2 12 0.23493577355184492 2 13 1.9460639558853803 2 14 -1.6651843692022252 2 15 2.1238707225314357 2 16 2.54327007318079 2 17 0.5065923907441752 2 18 2.5893011750154615 2 19 3.681881957328385 2 20 5.813471771284271 2 21 5.5205878942486075 2 22 1.640067447433517 2 23 6.071243364770148 2 24 2.567428429705215 2 25 7.953900613275613 2 26 6.429192434549577 2 27 3.7855020227788083 2 28 2.724945243248813 2 29 3.3984929009482565 2 30 5.104160224695941 2 31 4.5063740079365076 2 32 7.060533910533911 2 33 4.986528550814264 2 34 8.729378607503609 2 35 5.240664940218511 2 36 7.516803880643168 2 37 7.045188169964956 2 38 6.161380707586065 2 39 7.639019338796124 2 40 5.972825834879407 3 1 3.746095199443431 3 2 2.2060738121005983 3 3 4.608882511337871 3 4 5.149493339002266 3 5 6.655829661410014 3 6 -0.03718080035044302 3 7 -0.8493223046794416 3 8 2.9828952793238557 3 9 1.4222763347763347 3 10 2.166983933725003 3 11 -2.0179708436404864 3 12 1.9622085008245715 3 13 0.2551548649762889 3 14 -1.8288207328385884 3 15 -1.348856550195836 3 16 3.7978155277262458 3 17 1.6702287543805392 3 18 2.225664811379098 3 19 0.3182455936920219 3 20 4.540744498556998 3 21 4.193315166975881 3 22 3.4037038110698807 3 23 1.1439706374974223 3 24 3.7674284297052143 3 25 4.044809704184705 3 26 3.556465161822304 3 27 4.694592931869717 3 28 5.179490697794268 3 29 3.76212926458462 3 30 5.522342042877759 3 31 3.5063740079365076 3 32 4.3696248196248195 3 33 2.5683467326324463 3 34 5.711196789321791 3 35 4.567937667491237 3 36 7.189531153370441 3 37 6.936097260874049 3 38 7.870471616676974 3 39 8.420837520614306 3 40 5.809189471243043 4 1 2.170337623685853 4 2 5.822235428262214 4 3 6.49777140022676 4 4 7.149493339002266 4 5 10.29219329777365 4 6 -1.016978780148424 4 7 12.433505978148842 4 8 5.619258915687492 4 9 5.593993506493504 4 10 2.8437516104926797 4 11 4.295160469490826 4 12 3.234935773551845 4 13 3.3460639558853806 4 14 3.3125934085755535 4 15 6.923870722531436 4 16 6.676603406514125 4 17 3.4177035018552866 4 18 4.700412286126572 4 19 6.5707708462172745 4 20 3.5912495490620495 4 21 9.03169900535972 4 22 7.928956336322407 4 23 10.537910031436816 4 24 6.211872874149659 4 25 5.731678391053392 4 26 8.718081323438467 4 27 7.896613133889919 4 28 10.169389687693256 4 29 4.954048456503812 4 30 11.037493558029272 4 31 10.061929563492065 4 32 9.793867243867243 4 33 12.164306328592044 4 34 11.64048971861472 4 35 10.84066494021851 4 36 13.13902610286539 4 37 13.956299281076067 4 38 13.40582515203051 4 39 10.239019338796123 4 40 10.061714723768295 5 1 -11.877281423933194 5 2 -15.066653460626675 5 3 -13.391117488662129 5 4 -14.850506660997734 5 5 -14.70780670222635 5 6 -10.128089891259535 5 7 -11.122049577406715 5 8 -9.380741084312508 5 9 -8.850450937950939 5 10 -8.378470611729542 5 11 -5.927061752731396 5 12 -5.765064226448155 5 13 -5.653936044114619 5 14 -5.465184369202225 5 15 -5.076129277468564 5 16 -3.6567299268192093 5 17 -3.693407609255824 5 18 -3.4106988249845385 5 19 -3.318118042671615 5 20 -3.186528228715729 5 21 -3.079412105751392 5 22 -2.9599325525664826 5 23 -3.128756635229851 5 24 -3.2325715702947857 5 25 -3.0460993867243866 5 26 -3.1708075654504224 5 27 -3.2144979772211917 5 28 -3.275054756751187 5 29 -2.6015070990517435 5 30 -3.295839775304059 5 31 -3.4936259920634924 5 32 -3.5394660894660888 5 33 -3.6134714491857354 5 34 -3.4706213924963905 5 35 -3.1593350597814895 5 36 -3.083196119356832 5 37 -3.154811830035043 5 38 -3.0386192924139346 5 39 -2.7609806612038765 5 40 -2.827174165120594 6 1 2.503670957019189 6 2 5.266679872706657 6 3 -5.724450821995463 6 4 3.3161600056689338 6 5 1.9588599644403182 6 6 -0.9614232245928687 6 7 10.711283755926617 6 8 3.1192589156874924 6 9 2.4828823953823953 6 10 2.1215293882704582 6 11 0.7396049139352705 6 12 3.568269106885179 6 13 2.5127306225520467 6 14 2.868148964131109 6 15 4.25720405586477 6 16 4.676603406514125 6 17 4.306592390744176 6 18 8.755967841682128 6 19 8.681881957328386 6 20 1.313471771284271 6 21 9.420587894248609 6 22 6.7067341141001835 6 23 3.704576698103483 6 24 2.1007617630385482 6 25 3.6205672799422794 6 26 7.495859101216244 6 27 7.285502022778808 6 28 5.224945243248813 6 29 5.3984929009482565 6 30 7.204160224695941 6 31 8.006374007936508 6 32 7.460533910533911 6 33 10.553195217480933 6 34 9.862711940836942 6 35 10.84066494021851 6 36 7.583470547309834 6 37 4.345188169964957 6 38 7.6280473742527315 6 39 9.739019338796123 6 40 8.672825834879406 7 1 4.170337623685857 7 2 -0.39998679396000725 7 3 5.442215844671203 7 4 -1.017173327664402 7 5 4.79219329777365 7 6 -6.628089891259535 7 7 -6.288716244073381 7 8 0.4525922490208263 7 9 -5.850450937950939 7 10 1.2881960549371243 7 11 -5.927061752731396 7 12 -5.765064226448155 7 13 -5.653936044114619 7 14 -5.465184369202225 7 15 -5.076129277468564 7 16 -3.6567299268192093 7 17 -3.693407609255824 7 18 -3.4106988249845385 7 19 -3.318118042671615 7 20 -3.186528228715729 7 21 -3.079412105751392 7 22 -2.9599325525664826 7 23 -3.128756635229851 7 24 -3.2325715702947857 7 25 -3.0460993867243866 7 26 -3.1708075654504224 7 27 -3.2144979772211917 7 28 -3.275054756751187 7 29 -2.6015070990517435 7 30 -3.295839775304059 7 31 -3.4936259920634924 7 32 -3.5394660894660888 7 33 -3.6134714491857354 7 34 -3.4706213924963905 7 35 -3.1593350597814895 7 36 -3.083196119356832 7 37 -3.154811830035043 7 38 -3.0386192924139346 7 39 -2.7609806612038765 7 40 -2.827174165120594 8 1 -6.787995709647479 8 2 -5.566653460626675 8 3 -3.891117488662129 8 4 -11.100506660997734 8 5 -9.95780670222635 8 6 -2.5030898912595347 8 7 -6.247049577406715 8 8 -4.880741084312508 8 9 -6.100450937950939 8 10 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13.78722396155432 9 12 11.806364344980418 9 13 9.774635384456808 9 14 3.6776727736549173 9 15 9.638156436817152 9 16 9.34327007318079 9 17 7.878020962172747 9 18 7.5893011750154615 9 19 10.824739100185528 9 20 8.670614628427128 9 21 6.7777307513914655 9 22 4.754353161719231 9 23 7.728386221913007 9 24 8.910285572562357 9 25 13.811043470418472 9 26 10.257763863121005 9 27 12.35693059420738 9 28 11.15351667182024 9 29 5.827064329519685 9 30 10.132731653267367 9 31 10.363516865079365 9 32 11.889105339105338 9 33 10.386528550814266 9 34 11.815092893217894 9 35 9.84066494021851 9 36 13.059661023500311 9 37 13.988045312822099 9 38 12.104237850443209 9 39 12.09616219593898 9 40 14.172825834879406 10 1 -15.912995709647479 10 2 -6.816653460626675 10 3 -6.891117488662129 10 4 -7.350506660997734 10 5 -6.70780670222635 10 6 -3.6280898912595347 10 7 -7.622049577406715 10 8 -4.880741084312508 10 9 -5.850450937950939 10 10 -1.3784706117295418 10 11 -3.9270617527313956 10 12 -3.515064226448155 10 13 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11.234935773551845 11 13 12.060349670171096 11 14 10.534815630797775 11 15 13.49529929396001 11 16 15.057555787466505 11 17 13.306592390744175 11 18 11.732158317872603 11 19 15.539024814471244 11 20 14.384900342712843 11 21 11.634873608534324 11 22 9.611496018862091 11 23 10.728386221913006 11 24 12.195999858276641 11 25 6.382472041847042 11 26 12.829192434549578 11 27 12.214073451350234 11 28 9.867802386105955 11 29 10.541350043805398 11 30 6.275588796124512 11 31 10.934945436507935 11 32 8.603391053391054 11 33 12.815099979385693 11 34 13.243664321789325 11 35 12.983522083075652 11 36 10.631089594928884 11 37 10.845188169964956 11 38 9.961380707586066 11 39 11.239019338796123 11 40 9.45854012059369 12 1 2.6941471474953786 12 2 10.076203682230467 12 3 5.466025368480729 12 4 5.006636196145124 12 5 10.435050440630793 12 6 0.5861958230261788 12 7 9.020807565450427 12 8 6.190687487116065 12 9 7.578120490620488 12 10 10.907243673984743 12 11 7.5015096758400315 12 12 9.806364344980418 12 13 8.34606395588538 12 14 6.534815630797775 12 15 5.63815643681715 12 16 2.486127216037933 12 17 3.5923066764584624 12 18 10.446444032158318 12 19 7.396167671614099 12 20 6.5277574855699845 12 21 4.634873608534321 12 22 5.468638876004946 12 23 7.728386221913007 12 24 6.481714143990928 12 25 4.382472041847042 12 26 7.400621005978149 12 27 6.928359165635951 12 28 6.867802386105955 12 29 2.3984929009482565 12 30 5.5613030818387985 12 31 8.363516865079365 12 32 8.889105339105338 12 33 7.100814265099978 12 34 5.957950036075038 12 35 2.2692363687899393 12 36 2.345375309214597 12 37 3.845188169964957 12 38 2.5328092790146366 12 39 6.52473362451041 12 40 4.4585401205936925 13 1 8.281448734796964 13 2 15.04445765048444 13 3 8.38666028911565 13 4 13.593937783446709 13 5 12.514415519995872 13 6 5.871910108740465 13 7 5.433505978148842 13 8 6.1748144712430495 13 9 4.4828823953823935 13 10 7.288196054937126 13 11 8.961827136157494 13 12 7.0127135513296235 13 13 5.568286178107602 13 14 12.423704519686664 13 15 11.146092944753658 13 16 3.009936739847457 13 17 2.8621479462997312 13 18 5.9226345083487955 13 19 5.015215290661719 13 20 6.924582882395382 13 21 0.5872545609152748 13 22 1.3734007807668513 13 23 0.4267989203257043 13 24 0.9896506519274357 13 25 1.8427895021645027 13 26 2.051414656771799 13 27 0.7855020227788083 13 28 2.724945243248813 13 29 1.8429373453927012 13 30 2.259715780251496 13 31 3.5063740079365076 13 32 1.0160894660894666 13 33 3.275417439703154 13 34 2.418267496392499 13 35 4.062887162440732 13 36 3.583470547309834 13 37 3.511854836631623 13 38 2.40582515203051 13 39 2.1279082276850128 13 40 3.2839369459905168 14 1 -13.762995709647479 14 2 -9.866653460626676 14 3 -13.391117488662129 14 4 -14.850506660997734 14 5 -14.70780670222635 14 6 -10.128089891259535 14 7 -11.122049577406715 14 8 -9.380741084312508 14 9 -8.850450937950939 14 10 -8.378470611729542 14 11 -5.927061752731396 14 12 -5.765064226448155 14 13 -5.653936044114619 14 14 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-5.465184369202225 116 15 -5.076129277468564 116 16 -3.6567299268192093 116 17 -3.693407609255824 116 18 -3.4106988249845385 116 19 -3.318118042671615 116 20 -3.186528228715729 116 21 -3.079412105751392 116 22 -2.9599325525664826 116 23 -3.128756635229851 116 24 -3.2325715702947857 116 25 -3.0460993867243866 116 26 -3.1708075654504224 116 27 -3.2144979772211917 116 28 -3.275054756751187 116 29 -2.6015070990517435 116 30 -3.295839775304059 116 31 -3.4936259920634924 116 32 -3.5394660894660888 116 33 -3.6134714491857354 116 34 -3.4706213924963905 116 35 -3.1593350597814895 116 36 -3.083196119356832 116 37 -3.154811830035043 116 38 -3.0386192924139346 116 39 -2.7609806612038765 116 40 -2.827174165120594 117 1 5.837004290352521 117 2 7.683346539373325 117 3 6.358882511337871 117 4 8.399493339002266 117 5 8.79219329777365 117 6 6.371910108740465 117 7 5.627950422593285 117 8 13.369258915687492 117 9 14.399549062049061 117 10 -0.6284706117295418 117 11 0.07293824726860443 117 12 0.23493577355184492 117 13 -4.403936044114619 117 14 0.5348156307977749 117 15 1.6738707225314364 117 16 -0.6567299268192093 117 17 -0.19340760925582412 117 18 0.08930117501546153 117 19 -0.06811804267161481 117 20 1.313471771284271 117 21 -0.8294121057513921 117 22 1.5400674474335174 117 23 -0.878756635229851 117 24 1.7674284297052143 117 25 0.9539006132756134 117 26 1.3291924345495776 117 27 1.7855020227788083 117 28 0.47494524324881304 117 29 1.8984929009482565 117 30 1.4541602246959409 117 31 4.0063740079365076 117 32 1.9605339105339112 117 33 1.8865285508142646 117 34 3.7793786075036095 117 35 2.5906649402185105 117 36 0.916803880643168 117 37 3.345188169964957 117 38 -3.0386192924139346 117 39 -2.7609806612038765 117 40 -2.827174165120594 119 1 7.337004290352521 119 2 9.266679872706657 119 3 6.108882511337871 119 4 9.316160005668934 119 5 6.458859964440318 119 6 11.538576775407133 119 7 13.711283755926617 119 8 12.452592249020825 119 9 10.482882395382394 119 10 12.288196054937126 119 11 10.739604913935272 119 12 10.734935773551845 119 13 13.84606395588538 119 14 10.034815630797775 119 15 7.090537389198104 119 16 2.8432700731807907 119 17 2.473259057410842 119 18 2.0893011750154615 119 19 1.0152152906617191 119 20 4.646805104617605 119 21 2.587254560915274 119 22 4.040067447433517 119 23 2.537910031436815 119 24 -0.39923823696145266 119 25 -1.7127660533910536 119 26 -0.17080756545042242 119 27 1.6188353561121422 119 28 -0.10838809008452 119 29 0.23182623428158955 119 30 -2.129173108637392 119 31 0.8397073412698415 119 32 0.7938672438672452 119 33 1.3865285508142646 119 34 0.5293786075036095 119 35 2.1739982735518444 119 36 2.083470547309834 119 37 1.845188169964957 119 38 2.1280473742527315 119 39 -0.5943139945372096 119 40 1.50615916821274 120 1 0.837004290352521 120 2 3.7333465393733256 120 3 5.408882511337872 120 4 7.149493339002266 120 5 3.2921932977736503 120 6 1.471910108740465 120 7 5.677950422593286 120 8 11.619258915687492 120 9 6.54954906204906 120 10 6.8215293882704575 120 11 -0.3270617527313959 120 12 -0.16506422644815544 120 13 0.14606395588538135 120 14 -5.465184369202225 120 15 -5.076129277468564 120 16 -3.6567299268192093 120 17 -3.693407609255824 120 18 -3.4106988249845385 120 19 -3.318118042671615 120 20 -3.186528228715729 120 21 -3.079412105751392 120 22 -2.9599325525664826 120 23 -3.128756635229851 120 24 -3.2325715702947857 120 25 -3.0460993867243866 120 26 -3.1708075654504224 120 27 -3.2144979772211917 120 28 -3.275054756751187 120 29 -2.6015070990517435 120 30 -3.295839775304059 120 31 -3.4936259920634924 120 32 -3.5394660894660888 120 33 -3.6134714491857354 120 34 -3.4706213924963905 120 35 -3.1593350597814895 120 36 -3.083196119356832 120 37 -3.154811830035043 120 38 -3.0386192924139346 120 39 -2.7609806612038765 120 40 -2.827174165120594 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1598.0 5 212.0 6 420.0 7 655.0 8 423.0 9 370.0 10 328.0 11 234.0 12 174.0 13 128.0 14 90.0 15 73.0 16 50.0 17 59.0 18 42.0 19 73.0 20 130.0 21 169.0 22 163.0 23 184.0 24 144.0 25 122.0 26 80.0 27 58.0 28 32.0 29 17.0 30 5.0 31 1.0 32 1.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.4364329520968 0.34808552958727 0.0828775070445881 0.13260401127134097 8 97.52779160444666 1.2609922017587523 0.49776007964161273 0.7134561141529783 9 93.40860036526648 2.507056284243732 2.7228955669931927 1.3614477834965963 10 38.17970822281167 28.000663129973475 19.03183023872679 14.787798408488065 11 25.418392709196354 29.776304888152445 22.734051367025685 22.071251035625515 12 26.53737775567711 26.338471738770096 22.957069451350904 24.167081054201887 13 18.96866191344719 24.125352346211244 24.672525286022218 32.23346045431935 14 18.373094764744863 34.940357852882705 28.24718356527502 18.439363817097416 15 34.57587806494367 26.640159045725646 22.69715043074884 16.086812458581843 16 27.76673293571902 32.77004638833665 24.801192842942346 14.66202783300199 17 23.317865429234338 25.190586675505465 35.067948293006296 16.423599602253894 18 20.990722332670643 25.62955599734924 30.997349237905897 22.38237243207422 19 24.90053050397878 28.763262599469495 24.6684350132626 21.667771883289124 20 20.957587806494367 32.05765407554672 30.583167660702454 16.40159045725646 21 19.367130550033135 25.62955599734924 31.096752816434726 23.906560636182903 22 18.455931080185554 24.055666003976143 31.676607024519548 25.811795891318756 23 16.746808157851103 29.08307080086221 30.873818603879954 23.296302437406734 24 17.08084824387011 28.76076872100729 30.59973492379059 23.55864811133201 25 26.97281167108753 30.03978779840849 26.923076923076923 16.064323607427056 26 26.703223662346293 29.677633765370555 20.771020272515788 22.848122299767365 27 18.193913188092466 33.67703309496092 21.836021952436386 26.29303176451023 28 22.877984084880637 37.317639257294424 22.579575596816976 17.22480106100796 29 29.13621262458472 31.328903654485053 22.27574750830565 17.259136212624586 30 23.15213788531654 28.256546237984754 31.985415976135233 16.60589990056347 31 23.669816011934362 36.13459307144041 23.073097961213325 17.1224929554119 32 28.27712244558897 30.08805449410201 24.106994517361688 17.527828542947333 33 23.31013916500994 31.01391650099404 24.271040424121935 21.40490390987409 34 19.897282968853546 32.95228628230616 30.268389662027833 16.882041086812457 35 17.693836978131213 29.605699138502317 37.04440026507621 15.656063618290258 36 20.500912257422456 34.21794659147454 26.40570575551501 18.875435395587992 37 21.47117296222664 28.031809145129227 33.465871438038434 17.0311464546057 38 26.959403479701738 27.43993371996686 27.70505385252693 17.895608947804472 39 21.657000828500415 28.55012427506214 25.16984258492129 24.623032311516155 40 21.570576540755468 27.269715043074882 33.69781312127236 17.461895294897285 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 2.0 12 4.0 13 4.0 14 12.5 15 21.0 16 56.5 17 92.0 18 92.0 19 97.0 20 102.0 21 90.0 22 78.0 23 78.0 24 64.5 25 51.0 26 48.5 27 46.0 28 46.0 29 44.0 30 42.0 31 47.5 32 53.0 33 53.0 34 83.5 35 114.0 36 143.5 37 173.0 38 173.0 39 195.0 40 217.0 41 243.5 42 270.0 43 270.0 44 344.0 45 418.0 46 472.5 47 527.0 48 527.0 49 506.5 50 486.0 51 518.0 52 550.0 53 550.0 54 644.5 55 739.0 56 676.5 57 614.0 58 614.0 59 542.5 60 471.0 61 425.0 62 379.0 63 379.0 64 332.0 65 285.0 66 222.0 67 159.0 68 159.0 69 120.5 70 82.0 71 55.5 72 29.0 73 29.0 74 22.0 75 15.0 76 13.0 77 11.0 78 11.0 79 8.0 80 5.0 81 3.0 82 1.0 83 1.0 84 1.0 85 1.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.049701789264413515 8 0.14910536779324055 9 0.21537442014579194 10 0.06626905235255136 11 0.01656726308813784 12 0.049701789264413515 13 0.08283631544068919 14 0.0 15 0.0 16 0.0 17 0.03313452617627568 18 0.0 19 0.06626905235255136 20 0.0 21 0.0 22 0.0 23 0.08283631544068919 24 0.0 25 0.06626905235255136 26 0.2982107355864811 27 0.3810470510271703 28 0.06626905235255136 29 0.26507620941020543 30 0.03313452617627568 31 0.049701789264413515 32 0.28164347249834326 33 0.0 34 0.0 35 0.0 36 0.11597084161696489 37 0.0 38 0.01656726308813784 39 0.01656726308813784 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 6036.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.57720344599072 #Duplication Level Percentage of deduplicated Percentage of total 1 92.09991876523151 75.1325381047051 2 5.239642567018684 8.548707753479125 3 1.421608448415922 3.4791252485089466 4 0.4264825345247766 1.3916500994035785 5 0.2843216896831844 1.159708416169649 6 0.060926076360682375 0.2982107355864811 7 0.060926076360682375 0.34791252485089463 8 0.04061738424045491 0.26507620941020543 9 0.10154346060113728 0.7455268389662028 >10 0.18277822908204713 1.9383697813121274 >50 0.060926076360682375 3.4791252485089466 >100 0.020308692120227456 3.214049039098741 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 194 3.214049039098741 No Hit CGCTTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 83 1.3750828363154408 No Hit CGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 1.110006626905235 No Hit CGCTTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 60 0.9940357852882704 No Hit CGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 18 0.2982107355864811 No Hit CGCTTGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT 16 0.26507620941020543 No Hit CGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.2485089463220676 No Hit CGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.23194168323392977 No Hit CGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.21537442014579194 No Hit CGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 11 0.18223989396951623 No Hit CGCTTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC 10 0.16567263088137837 No Hit CGCTTGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 10 0.16567263088137837 No Hit CGCTTGGGGGTTTCTCATAGCCTCGTTGTGTGGCTTTGGC 10 0.16567263088137837 No Hit CGCTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 9 0.14910536779324055 No Hit CGCTTGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT 9 0.14910536779324055 No Hit CGCTTGGGGGTCATCTTCCAGGAACTGCTCACTGACAAGT 9 0.14910536779324055 No Hit CGCTTGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 9 0.14910536779324055 No Hit CGCTTGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 9 0.14910536779324055 No Hit CGCTTGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 8 0.13253810470510272 No Hit CGCTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG 8 0.13253810470510272 No Hit CGCTTGGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT 7 0.11597084161696489 No Hit CGCTTGGGGCAGACTTGTATTTACATCTCATAGATATTTT 7 0.11597084161696489 No Hit CGCTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 7 0.11597084161696489 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.01656726308813784 0.0 0.0 0.0 0.0 20 0.01656726308813784 0.0 0.0 0.0 0.0 21 0.01656726308813784 0.0 0.0 0.0 0.0 22 0.01656726308813784 0.0 0.0 0.0 0.0 23 0.01656726308813784 0.0 0.0 0.0 0.0 24 0.01656726308813784 0.0 0.0 0.0 0.0 25 0.01656726308813784 0.0 0.0 0.0 0.0 26 0.01656726308813784 0.0 0.0 0.0 0.0 27 0.01656726308813784 0.0 0.0 0.0 0.0 28 0.01656726308813784 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTGC 35 8.71325E-7 34.0 20 TGGTTGA 35 8.71325E-7 34.0 14 GGGTCAC 30 1.2536255E-5 34.0 7 GTCACAG 25 1.7985818E-4 34.0 9 GTACCTG 25 1.7985818E-4 34.0 9 CCAGTAG 35 8.71325E-7 34.0 26 AGTAGCA 30 1.2536255E-5 34.0 28 CCTGCCA 35 8.71325E-7 34.0 22 CAGTAGC 30 1.2536255E-5 34.0 27 TGATCCT 35 8.71325E-7 34.0 18 GGTCACA 25 1.7985818E-4 34.0 8 GCCAGTA 35 8.71325E-7 34.0 25 CTGGTTG 35 8.71325E-7 34.0 13 ACCTGGT 25 1.7985818E-4 34.0 11 TGGGGTC 45 4.1600288E-9 34.0 5 TGGGGTA 25 1.7985818E-4 34.0 5 TGGGGGG 85 0.0 34.0 5 TGGGGCG 30 1.2536255E-5 34.0 5 TGGGGAT 55 2.0008883E-11 34.0 5 GATCCTG 35 8.71325E-7 34.0 19 >>END_MODULE