FastQCFastQC Report
Fri 17 Jun 2016
SRR1526074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26993
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT3861.4300003704664173No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2851.0558292890749454No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG920.3408291038417367No Hit
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT890.3297151113251584No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC890.3297151113251584No Hit
GACACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA630.2333938428481458No Hit
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA590.21857518615937466No Hit
GACACTGGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC590.21857518615937466No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC530.1963472011262179No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC480.17782388026525398No Hit
GACACTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC390.14448190271551883No Hit
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT380.14077723854332605No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC350.12966324602674767No Hit
GACACTGGGACTTGAAACTAGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGC320.1185492535101693No Hit
GACACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA310.1148445893379765No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC310.1148445893379765No Hit
GACACTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC310.1148445893379765No Hit
GACACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA290.10743526099359092No Hit
GACACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT290.10743526099359092No Hit
GACACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT280.10373059682139812No Hit
GACACTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG270.10002593264920535No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCC203.7541776E-450.92395441
TGTCCAA203.7541776E-450.92395438
ACCGCGG150.0077579550.92395441
GTCCAAT203.7541776E-450.92395439
TCCAATC203.7541776E-450.92395440
GCATTGA150.0077579550.92395440
TTTTGTC309.0107096E-750.82732435
CGGCCAA251.9722827E-550.25515745
CGCGGCC251.9943765E-550.16104543
TGGTCTG150.00829761650.0672950
GACCGCA355.0549716E-849.97388512
AGACCGC355.0549716E-849.97388511
CATCCTC204.121217E-449.9738815
GCTCTTT204.121217E-449.9738810
ACCGCAC301.0133663E-649.97387713
CCACAGA150.00835925649.97387710
GACTTAA150.00835925649.97387710
GTACCTG355.1300958E-849.880829
GGACTTA150.00842123749.8808179
GGGCTAA150.00842123749.8808179
Produced by FastQC (version 0.11.5)