##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26993
Sequences flagged as poor quality	0
Sequence length	56
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.80820953580558	38.0	35.0	39.0	29.0	40.0
2	33.504390027044046	38.0	33.0	39.0	15.0	40.0
3	35.693068573333825	38.0	35.0	39.0	27.0	40.0
4	32.615381765642944	38.0	31.0	39.0	12.0	40.0
5	35.499759196828805	38.0	35.0	39.0	27.0	40.0
6	35.69162375430667	39.0	36.0	39.0	27.0	40.0
7	35.97232615863372	39.0	35.0	39.0	29.0	40.0
8	35.59156077501574	38.0	35.0	39.0	28.0	40.0
9	35.54106620234876	38.0	35.0	39.0	27.0	40.0
10	35.04167747193717	38.0	35.0	39.0	25.0	40.0
11	35.13633164153669	38.0	35.0	39.0	27.0	40.0
12	33.98962694031786	38.0	33.0	39.0	22.0	40.0
13	33.31900863186752	38.0	32.0	39.0	19.0	40.0
14	33.99774015485496	38.0	33.0	39.0	22.0	40.0
15	32.88797095543289	38.0	31.0	39.0	18.0	40.0
16	32.444115140962474	37.0	30.0	39.0	18.0	40.0
17	31.486570592375802	36.0	28.0	38.0	15.0	40.0
18	31.002630311562257	35.0	26.0	38.0	14.0	40.0
19	32.16256066387582	36.0	29.0	38.0	18.0	40.0
20	32.831734153299	37.0	30.0	39.0	21.0	40.0
21	33.17548994183677	38.0	31.0	39.0	19.0	40.0
22	32.54595635905605	38.0	30.0	39.0	17.0	40.0
23	33.17682362093876	38.0	31.0	39.0	19.0	40.0
24	32.49038639647316	37.0	30.0	39.0	17.0	40.0
25	33.01218834512652	38.0	31.0	39.0	19.0	40.0
26	33.21194383729115	38.0	32.0	39.0	18.0	40.0
27	33.51379987404142	38.0	33.0	39.0	20.0	40.0
28	33.53736153817656	38.0	33.0	39.0	22.0	40.0
29	33.01515207646427	38.0	32.0	39.0	18.0	40.0
30	32.86989219427259	38.0	31.0	39.0	17.0	40.0
31	33.57603823213426	38.0	33.0	39.0	20.0	40.0
32	33.518356610973214	38.0	33.0	39.0	20.0	40.0
33	33.23309746971437	38.0	33.0	39.0	19.0	40.0
34	33.091912718112106	38.0	33.0	39.0	18.0	40.0
35	33.03975104656763	38.0	33.0	39.0	18.0	40.0
36	32.467639758455896	37.0	32.0	39.0	15.0	40.0
37	32.18641870114474	36.0	31.0	38.0	11.0	40.0
38	31.538139517652723	36.0	30.0	38.0	4.0	40.0
39	30.359241284777536	35.0	27.0	38.0	4.0	40.0
40	28.40314155521802	34.0	21.0	38.0	4.0	39.0
41	26.468121364798282	32.0	16.0	38.0	4.0	39.0
42	27.334308894898676	32.0	20.0	38.0	4.0	39.0
43	27.554291853443484	33.0	21.0	38.0	4.0	39.0
44	26.528173971029528	32.0	17.0	38.0	4.0	39.0
45	27.484903493498315	33.0	22.0	38.0	4.0	39.0
46	25.548623717260032	30.0	16.0	36.0	4.0	39.0
47	24.578890823546846	29.0	10.0	36.0	4.0	39.0
48	25.13492386915126	30.0	15.0	36.0	4.0	39.0
49	24.250435298040234	29.0	9.0	36.0	4.0	39.0
50	24.922239099025674	30.0	11.0	36.0	4.0	39.0
51	25.165190975438076	31.0	4.0	36.0	4.0	39.0
52	24.990441966435743	31.0	4.0	36.0	4.0	39.0
53	24.80913570184863	31.0	4.0	36.0	4.0	39.0
54	24.443040788352537	30.0	4.0	36.0	4.0	39.0
55	24.161671544474494	30.0	4.0	36.0	4.0	39.0
56	22.914237024413737	29.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	5.2056216505845185
1	2	8.002479340392956
1	3	4.790792094998089
1	4	8.961318961722426
1	5	5.165220384161238
1	6	4.868413474072398
1	7	4.8869904814771274
1	8	5.3303449145821915
1	9	5.333694213420877
1	10	5.46958054691752
1	11	0.06655505751650281
1	12	-7.889098022098224
1	13	-20.137031404788452
1	14	-6.763513767639779
1	15	0.7359764726716875
1	16	-2.999374621790313
1	17	-8.50192374468055
1	18	-6.569725338071763
1	19	-2.4136955735572094
1	20	-1.8930495941051007
1	21	6.916626254479645
1	22	7.669488009520677
1	23	6.798438846259764
1	24	7.4333705920069235
1	25	6.196263592180202
1	26	6.938118561571795
1	27	6.445116651457873
1	28	6.760822063594038
1	29	7.454028762401098
1	30	7.372549251069092
1	31	6.130422801046606
1	32	5.950816838461577
1	33	6.204242756649553
1	34	5.969441069290895
1	35	7.226334024679268
1	36	5.0480386426335
1	37	4.622507856662903
1	38	4.289863790586502
1	39	5.708862069224164
1	40	-10.118824636025918
1	41	-0.6538073957876556
1	42	-3.046383625311808
1	43	-8.230306522570139
1	44	-15.046875285149072
1	45	-3.6155480898326715
1	46	-6.528221070286044
1	47	-9.508968190939724
1	48	-6.385004122455198
1	49	-14.011403059811087
1	50	-5.023814603407452
1	51	-0.27375779593159066
1	52	0.8929349118304692
1	53	0.8594452556943644
1	54	0.9670513314605707
1	55	1.465663028654287
1	56	4.313062739295983
2	1	5.391402657330758
2	2	7.7596147840878125
2	3	4.469048452029746
2	4	8.964951551239814
2	5	4.922355827856094
2	6	5.048486125862745
2	7	4.811225042971678
2	8	4.966048080124487
2	9	5.4743273218796205
2	10	4.5987969454644855
2	11	3.721459053364967
2	12	-0.30736475795707463
2	13	1.539668128164326
2	14	3.970788256231522
2	15	5.7370143553909365
2	16	4.368035860825152
2	17	6.416602461858108
2	18	3.703756758451334
2	19	6.0751471872108205
2	20	5.010427313004392
2	21	6.753678667556969
2	22	7.500313126282485
2	23	7.143534850411299
2	24	6.41105611354299
2	25	5.7100155382103
2	26	4.971849749947506
2	27	6.038266625510808
2	28	6.180126682172141
2	29	6.021750609832342
2	30	5.779918218375787
2	31	5.374844181430625
2	32	6.041112635036569
2	33	5.5467440540029465
2	34	5.267313409716429
2	35	5.776411865883212
2	36	6.911038123692144
2	37	6.964490212656681
2	38	6.93075636972506
2	39	6.914362847636202
2	40	3.4084197853544644
2	41	9.15885477338723
2	42	8.083351714594784
2	43	6.7779965391838815
2	44	-3.2004819275984744
2	45	6.731623679757366
2	46	5.565707315806328
2	47	3.213398181660171
2	48	3.536116790881593
2	49	-1.2226121931790175
2	50	4.444270502975527
2	51	7.534752842366281
2	52	7.412914154176086
2	53	7.908744684858867
2	54	6.886615420718485
2	55	7.241480361295697
2	56	9.141293149259656
3	1	4.774381380735015
3	2	7.291529677704833
3	3	4.383942069051024
3	4	8.539419636346196
3	5	3.986185615090136
3	6	4.1974222960755085
3	7	3.811225042971678
3	8	4.306473612039383
3	9	4.580710300603023
3	10	3.7264565199325688
3	11	3.2108207554926267
3	12	4.607528859064203
3	13	5.050306426036666
3	14	5.119724426444293
3	15	1.1199930787951935
3	16	6.751014584229409
3	17	7.140006717177261
3	18	7.42716101377048
3	19	7.138976974444862
3	20	5.691278376834184
3	21	6.79623185904633
3	22	7.479036530537805
3	23	6.292471020624063
3	24	7.155736964606817
3	25	6.22065383608264
3	26	6.525041239309207
3	27	5.697841093595912
3	28	5.605658597065759
3	29	6.553665503449363
3	30	6.843748005609829
3	31	4.630163330366791
3	32	5.08366582652593
3	33	5.163765330598697
3	34	4.948164473546214
3	35	5.967901227585344
3	36	6.63444237901129
3	37	6.624064680741785
3	38	7.611607433554845
3	39	8.510107528487264
3	40	9.153100636418298
3	41	12.520556901046806
3	42	10.93441554438202
3	43	11.629060368971114
3	44	10.522922327720671
3	45	11.455027935076515
3	46	13.012515826444623
3	47	13.255951373149532
3	48	13.557393386626277
3	49	13.181643125969916
3	50	13.188951354039354
3	51	11.683689012579048
3	52	12.455467345665447
3	53	12.440659578475891
3	54	12.758955846250402
3	55	12.36913993576378
3	56	12.609378255642639
4	1	4.4739143335113525
4	2	7.295162267222221
4	3	4.302987216949312
4	4	8.473514083673649
4	5	4.823756969527089
4	6	5.161096400901663
4	7	4.643088042452732
4	8	4.842540036533414
4	9	4.723938115859902
4	10	4.835434205454106
4	11	4.65192091117504
4	12	5.452365392535924
4	13	5.350773473260329
4	14	5.212095988457783
4	15	6.2237813507204685
4	16	7.585991231868228
4	17	7.741978694343839
4	18	9.137591735098972
4	19	7.708255645954981
4	20	6.887438210772949
4	21	7.209309181308917
4	22	7.108512399764578
4	23	5.895999821869523
4	24	7.16507790908009
4	25	7.0011416409606895
4	26	5.76738685425472
4	27	6.323165431945682
4	28	5.224236697740384
4	29	6.478419006303536
4	30	4.982305348630067
4	31	5.325544752266119
4	32	5.68252415553475
4	33	4.37497446396662
4	34	4.774319118071382
4	35	6.909260853947565
4	36	6.877306935316433
4	37	7.622507856662903
4	38	6.680107693025526
4	39	7.294227922882701
4	40	3.442150973730179
4	41	10.151070652992832
4	42	8.221909057615022
4	43	8.940425184746932
4	44	9.904344227046046
4	45	8.506403129679526
4	46	10.59373014922615
4	47	9.612983028572472
4	48	10.639386121447242
4	49	11.06176767189623
4	50	10.805453689275474
4	51	10.701851960165971
4	52	10.649032472806077
4	53	11.90822574349924
4	54	11.47924645341179
4	55	10.807126443288428
4	56	10.922818836856958
5	1	5.279953822477665
5	2	8.195278411240803
5	3	5.1038002250793895
5	4	9.132631389132413
5	5	5.238971835961465
5	6	5.231363532144407
5	7	5.007780260803493
5	8	5.095733997044448
5	9	5.363891658252463
5	10	5.505585192678268
5	11	5.410922072615222
5	12	5.620193499388421
5	13	6.21140065096068
5	14	6.321852086018758
5	15	7.125058934924883
5	16	7.307245587268923
5	17	8.526531539872298
5	18	10.02493203707342
5	19	8.58223938579238
5	20	7.612757606824047
5	21	7.068774918823429
5	22	7.844284757488161
5	23	6.882643259732475
5	24	6.1633357488013445
5	25	6.625415740844545
5	26	5.7975842990863065
5	27	5.972410495824889
5	28	5.402517766265351
5	29	5.472611805374388
5	30	6.08741568544772
5	31	5.711723614054733
5	32	5.8248005782989765
5	33	5.910979109727368
5	34	5.469441069290895
5	35	5.440039018872071
5	36	7.355820291878565
5	37	7.2299410738522205
5	38	7.905427089076625
5	39	8.43766578583276
5	40	10.296970950501372
5	41	12.878712929415595
5	42	12.327019394432675
5	43	12.429972223074461
5	44	11.868920301378218
5	45	12.365868867194038
5	46	13.68374176362801
5	47	13.125758870616604
5	48	14.306633508206826
5	49	13.357934919282986
5	50	12.119042539449694
5	51	12.821480299306504
5	52	12.918486595918736
5	53	12.72181459367346
5	54	12.051255744933279
5	55	12.661946420059625
5	56	10.610391426868574
6	1	4.53211832464217
6	2	7.690949406911802
6	3	4.7098608311399985
6	4	8.656440912941939
6	5	4.69459954158917
6	6	4.81686136764224
6	7	4.641979395002622
6	8	5.067595468905921
6	9	5.101986896347704
6	10	5.572684759777836
6	11	4.666333328026475
6	12	4.0011458803408
6	13	6.178933118493145
6	14	5.662761176927852
6	15	6.736530796396739
6	16	7.583219613242946
6	17	7.602289115629869
6	18	8.451338963480346
6	19	7.8019363554893495
6	20	5.873580117646561
6	21	6.784142884191397
6	22	7.0791332423366455
6	23	5.9032060302952445
6	24	6.796452631918228
6	25	6.516108381537187
6	26	5.4902249917270005
6	27	4.568730842145236
6	28	4.757496121243708
6	29	4.825425658188241
6	30	5.487848585880624
6	31	4.726875129206249
6	32	3.5444975479959453
6	33	3.7735332222814755
6	34	4.219441069290895
6	35	5.361034689867736
6	36	7.088504274562553
6	37	6.657430251341399
6	38	6.938976872626412
6	39	7.614072712239686
6	40	4.753680907211333
6	41	11.507500808203478
6	42	10.000179567592852
6	43	10.31958261268485
6	44	10.416539348997269
6	45	9.808509559834732
6	46	11.94239977228602
6	47	12.126841121698856
6	48	11.91377636534968
6	49	12.955337516685585
6	50	12.862549032956181
6	51	12.521696749522956
6	52	11.943378370810514
6	53	11.556230178089042
6	54	11.799091242768775
6	55	9.868656376769586
6	56	11.550867617344764
7	1	4.486663779187623
7	2	6.963676679639075
7	3	4.050769922049092
7	4	7.360986367487392
7	5	3.490054087043717
7	6	3.70322500400588
7	7	4.323797576820809
7	8	4.635777287087734
7	9	4.57925962362043
7	10	4.4817756688687425
7	11	2.166333328026475
7	12	3.842054971249894
7	13	4.974387663947692
7	14	2.685488449655125
7	15	4.736530796396739
7	16	6.651401431424766
7	17	6.329561842902596
7	18	7.814975327116706
7	19	6.415572719125716
7	20	6.282671026737468
7	21	6.170506520555037
7	22	6.3973150605184586
7	23	6.039569666658878
7	24	7.205543541009142
7	25	5.78883565426446
7	26	5.603861355363367
7	27	5.568730842145236
7	28	5.257496121243708
7	29	4.393607476370054
7	30	5.4196667676988035
7	31	4.181420583751702
7	32	3.635406638905039
7	33	3.682624131372389
7	34	4.128531978381801
7	35	2.9973983262313766
7	36	4.747595183653459
7	37	5.180157524068672
7	38	5.279885963535499
7	39	6.364072712239686
7	40	7.117317270847696
7	41	10.757500808203478
7	42	8.863815931229212
7	43	8.955946249048484
7	44	9.461993894451815
7	45	8.399418650743826
7	46	10.078763408649653
7	47	10.46775021260795
7	48	10.118321819895133
7	49	9.341701153049222
7	50	10.589821760228915
7	51	9.3171512949775
7	52	8.625196552628697
7	53	9.238048359907225
7	54	9.480909424586955
7	55	7.0959291040423125
7	56	4.778140344617491
8	1	4.749795092318941
8	2	6.522262538224933
8	3	4.226022447301617
8	4	8.556440912941937
8	5	4.3072258042154346
8	6	4.6012048019856735
8	7	4.560161213184443
8	8	4.765575266885719
8	9	4.690875785236592
8	10	5.137331224424301
8	11	4.174414136107288
8	12	4.590034769229689
8	13	6.163781603341629
8	14	5.444074308240978
8	15	6.055217665083607
8	16	6.967563047586381
8	17	7.693198206538963
8	18	8.0058844180258
8	19	6.948906052459048
8	20	5.861963956030401
8	21	5.662425712474224
8	22	7.05539586859927
8	23	5.623913101002316
8	24	6.6252405107061065
8	25	4.53176494719375
8	26	5.969012870514877
8	27	4.747013670428068
8	28	5.2675971313447185
8	29	4.834516567279149
8	30	5.663606161638196
8	31	4.611723614054739
8	32	5.3248005782989765
8	33	4.220502919251178
8	34	4.524996624846452
8	35	4.757499336332387
8	36	4.485979022037299
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115	36	7.094867910926183
115	37	7.574702978614123
115	38	8.343522327171868
115	39	7.998618166785139
115	40	10.584589998120425
115	41	9.592046262748934
115	42	9.596543203956486
115	43	6.621400794503032
115	44	0.1365393489972675
115	45	8.403964105289276
115	46	5.654217954104201
115	47	-5.610431605573872
115	48	4.5837763653496815
115	49	-1.9392079378598694
115	50	4.776185396592549
115	51	6.086242204068409
115	52	6.966105643537787
115	53	5.798957450816314
115	54	8.10363669731423
115	55	6.229565467678675
115	56	9.720867617344766
116	1	5.085148627672474
116	2	7.403070619033013
116	3	4.8159214372006005
116	4	8.095834852335877
116	5	5.050660147649779
116	6	5.082012882793755
116	7	4.960161213184442
116	8	5.203959105269554
116	9	5.450471744832555
116	10	5.254502941596016
116	11	3.711787873481022
116	12	3.5617519409468628
116	13	4.103175542735574
116	14	4.912761176927852
116	15	-3.0134692036032575
116	16	4.181704461727797
116	17	6.026531539872298
116	18	5.921035933177315
116	19	5.961027264580263
116	20	4.4417619358283815
116	21	7.185658035706545
116	22	7.556405969609372
116	23	7.062296939386151
116	24	7.152513237978837
116	25	6.069138684567491
116	26	6.520528022030032
116	27	6.288427811842205
116	28	6.5302233939709815
116	29	6.992092324854905
116	30	5.063606161638194
116	31	6.060208462539585
116	32	5.59752785102625
116	33	6.402321101069358
116	34	6.48459258444241
116	35	6.20194378077683
116	36	7.7324436685019435
116	37	7.771066614977759
116	38	8.385946569596108
116	39	9.31861816678514
116	40	11.223377876908298
116	41	9.227197777900447
116	42	8.181997749411032
116	43	1.6832189763212142
116	44	-0.4925515600936414
116	45	6.694873196198369
116	46	2.404520984407231
116	47	-6.8504316055738705
116	48	1.2092309108042265
116	49	-3.711329149981079
116	50	1.733761154168306
116	51	4.786848264674472
116	52	5.905499582931725
116	53	4.654715026573889
116	54	6.261212454889986
116	55	5.10350486161807
116	56	8.4372312537084
117	1	4.5014859135749745
117	2	6.795692489915755
117	3	4.024090080151858
117	4	7.547745260768025
117	5	4.2193030989014275
117	6	3.981881130488091
117	7	3.4819003436192233
117	8	4.389729856257695
117	9	4.341117331130313
117	10	4.7643843645209145
117	11	2.356056648184584
117	12	-3.629288902267895
117	13	-3.9811459328902536
117	14	0.12619991210571158
117	15	-3.4008209822593862
117	16	-6.469151928259031
117	17	0.7801547282780916
117	18	-0.7680286254524624
117	19	1.980790110430064
117	20	0.7856354536149404
117	21	4.654696243875193
117	22	6.054429685024388
117	23	3.378502472982987
117	24	5.96147239476408
117	25	5.307610357821773
117	26	5.731331711094594
117	27	4.634936375742072
117	28	4.4946502714413334
117	29	2.9736470020617567
117	30	4.39693949497153
117	31	2.6320134691272017
117	32	2.5711773898931796
117	33	3.930647846787405
117	34	2.665093243203941
117	35	3.767161172081181
117	36	5.105302693534881
117	37	4.526007326440212
117	38	6.187000588041428
117	39	7.57948773200253
117	40	9.127198693772595
117	41	7.146828871444583
117	42	6.245238856130399
117	43	-3.9333818141926216
117	44	-2.5834606510027314
117	45	4.777877148767541
117	46	-0.06056465459145244
117	47	1.4104379596435201
117	48	0.09855897404533209
117	49	-2.9339905465555205
117	50	3.0196636574621145
117	51	6.161024812764062
117	52	7.357409991363873
117	53	3.165913972555444
117	54	6.329723653835966
117	55	3.405217641591719
117	56	8.19217196517085
118	1	4.284094609227154
118	2	6.969605533394013
118	3	3.8501770366735926
118	4	7.765136565115853
118	5	4.2193030989014275
118	6	4.068837652227224
118	7	4.003639474054005
118	8	4.433208117127265
118	9	4.471552113739008
118	10	4.7643843645209145
118	11	1.7908392568802327
118	12	-2.3684193370505042
118	13	-4.63331984593373
118	14	-4.395539218329066
118	15	-9.052994895302863
118	16	-5.425673667389464
118	17	0.6497199456693963
118	18	-2.5071590602350717
118	19	-0.4974707591351546
118	20	-0.6056688942111457
118	21	5.567739722136061
118	22	5.228342728502653
118	23	4.682850299069948
118	24	4.787559351285822
118	25	5.046740792604382
118	26	5.166114319790243
118	27	4.15667550617686
118	28	5.059867662745685
118	29	3.8432122194530614
118	30	4.831722103667179
118	31	4.762448251735897
118	32	4.440742607284484
118	33	4.626300020700452
118	34	3.7520497649430666
118	35	5.723682911211611
118	36	5.670520084839232
118	37	6.221659500353258
118	38	6.273957109780561
118	39	8.927313818959053
118	40	10.953285650294337
118	41	8.755524523618494
118	42	7.245238856130399
118	43	1.5014007945030308
118	44	1.0252350011711826
118	45	8.995268453115365
118	46	0.1133483888868092
118	47	1.627829263991348
118	48	-0.37970189551988653
118	49	1.0660094534444795
118	50	2.7153158313751575
118	51	9.856676986677105
118	52	9.357409991363873
118	53	7.20939223342501
118	54	8.634071479922923
118	55	9.231304598113457
118	56	11.93130239995346
119	1	3.910906203430052
119	2	6.391706982669373
119	3	3.3538002250793895
119	4	6.781440912941939
119	5	3.096114693104326
119	6	3.820649246430122
119	7	3.335161213184442
119	8	3.6850197113301633
119	9	3.9353202296810395
119	10	4.273442335535407
119	11	0.529969691662842
119	12	-7.3738541196592
119	13	-1.9195517299917029
119	14	-6.96981458064791
119	15	-8.6308934460275
119	16	-8.496325841302507
119	17	-0.3068017934610374
119	18	-3.302448915307533
119	19	-0.5010939475409515
119	20	-0.9824804884140441
119	21	4.515203490252006
119	22	4.385951424154825
119	23	5.16835754544676
119	24	4.133573844039439
119	25	4.053987169415976
119	26	3.8273462038482116
119	27	4.424791448205845
119	28	5.003708242455826
119	29	4.567849900612483
119	30	5.230272828304866
119	31	4.086723614054733
119	32	3.658133911632312
119	33	3.5121695859178423
119	34	3.5944410692908946
119	35	3.6602771141101655
119	36	4.76653457759285
119	37	3.80136964528079
119	38	4.3935223271718655
119	39	4.693618166785139
119	40	5.886256664787091
119	41	3.795379596082263
119	42	1.1365432039564851
119	43	-1.8735992054969692
119	44	-3.250127317669399
119	45	3.770630771955947
119	46	-1.292448712562468
119	47	-2.9754316055738705
119	48	-5.33622363465032
119	49	-2.2075412711932003
119	50	0.5178520632592161
119	51	4.226242204068409
119	52	4.007772310204455
119	53	3.2456241174829827
119	54	3.4203033639808957
119	55	4.251232134345344
119	56	6.009200950678096
120	1	3.41848196100581
120	2	5.509131225093615
120	3	2.8462244675036317
120	4	6.792804549305572
120	5	3.0809631779528104
120	6	3.475952276733153
120	7	2.960161213184442
120	8	3.567595468905921
120	9	3.6019868963477037
120	10	3.4363211234141957
120	11	1.529969691662842
120	12	-1.4988541196591996
120	13	-6.411975972415945
120	14	-6.223602459435789
120	15	-3.9225601126941676
120	16	-9.530416750393417
120	17	-1.2158927025519475
120	18	-4.366842854701474
120	19	-1.857154553601557
120	20	-1.6946017005352587
120	21	2.7159610660095765
120	22	2.601860515063919
120	23	3.1986605757497912
120	24	2.7282708137364082
120	25	2.584290199719007
120	26	4.081134082636094
120	27	2.955094478508876
120	28	2.757496121243708
120	29	4.189062021824601
120	30	3.760575858607897
120	31	3.726875129206249
120	32	3.521770275268679
120	33	3.8871695859178423
120	34	3.1058047056545277
120	35	2.4292165080495565
120	36	3.8839588200170923
120	37	2.3165211604323055
120	38	4.416249599899139
120	39	5.227709075876046
120	40	7.026408179938603
120	41	-2.9697719190692524
120	42	-2.1816386142253315
120	43	-4.589508296406059
120	44	-13.583460651002731
120	45	0.9676004689256423
120	46	-7.580327500441255
120	47	-3.2595225146647806
120	48	-2.972587271013957
120	49	-13.044662483314413
120	50	0.33982176022891153
120	51	4.817151294977499
120	52	6.193378370810514
120	53	5.8971392689981315
120	54	3.412727606405138
120	55	3.209565467678676
120	56	6.300867617344764
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	695.0
5	26.0
6	54.0
7	65.0
8	85.0
9	118.0
10	104.0
11	68.0
12	77.0
13	51.0
14	77.0
15	107.0
16	136.0
17	180.0
18	196.0
19	240.0
20	368.0
21	404.0
22	570.0
23	623.0
24	648.0
25	741.0
26	851.0
27	865.0
28	973.0
29	973.0
30	1100.0
31	1200.0
32	1407.0
33	1496.0
34	1711.0
35	1883.0
36	2106.0
37	2334.0
38	2387.0
39	2073.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.41466306079354	0.4704923498684844	0.08520727596043418	0.029637313377542326
8	99.56655429185345	0.29637313377542324	0.07409328344385581	0.06297929092727744
9	98.58852295039455	0.3704664172192791	0.7187048494054015	0.3223057829807728
10	52.38395139480606	18.760419367984294	18.078761160300818	10.776868076908828
11	30.311562256881412	24.632312080909866	17.9342792575853	27.121846404623422
12	28.325862260586078	24.72863334938688	19.957025895602566	26.988478494424477
13	20.249694365205794	18.856740636461307	22.805912644018818	38.08765235431408
14	19.801430000370466	30.65609602489534	25.276923646871413	24.26555032786278
15	31.68943313358395	20.322328507090486	22.3768935869282	25.611344772397366
16	25.653382761816495	30.6468952734013	22.40593141797961	21.293790546802597
17	24.012597258243794	21.259725824379398	32.534271952575025	22.19340496480178
18	23.280109658059498	19.293891008780054	22.505834846071203	34.920164487089245
19	23.218322647592927	19.5789941815217	21.70996553385465	35.49271763703072
20	24.084449556602724	21.134651775444325	32.64813921561352	22.13275945233943
21	24.59897010336013	19.267958359574706	23.55055014262957	32.582521394435595
22	22.746638017263734	19.41984959063461	33.25306560960249	24.580446782499166
23	22.557700144481903	22.042751824547103	32.652910013707256	22.746638017263734
24	20.308968991960878	23.528322157596413	22.694772718853034	33.46793613158967
25	21.63313697158312	23.511540883998368	30.810270090030013	24.0450520543885
26	34.32160990253122	23.707519549345886	20.383204239706483	21.587666308416413
27	23.378308251167617	31.61094224924012	21.07643264882497	23.934316850767292
28	28.963064498203238	29.148297706812876	19.26054903123032	22.628088763753563
29	30.305728702879193	28.33555951320867	19.809290590679726	21.549421193232412
30	27.936107919801355	22.551235963384354	28.87002927769336	20.64262683912093
31	30.42833852082407	27.2750852230621	21.0204535349044	21.276122721209425
32	31.146022453592202	28.541257549371966	20.022972322057132	20.289747674978695
33	31.01568583824675	27.040456854674233	20.080097897430193	21.863759409648832
34	23.442543875922972	22.236651701235576	33.20470483470001	21.11609958814144
35	22.323614511462274	24.33785889160917	34.67616292009793	18.662363676830626
36	27.063097797879436	23.75620968339883	27.36338696522577	21.81730555349596
37	27.57418397626113	23.35311572700297	27.818991097922847	21.253709198813056
38	29.216152019002372	23.21481591448931	27.353028503562943	20.216003562945367
39	28.35361329574121	22.963347677696987	22.139783350645498	26.54325567591631
40	24.730861244019138	18.71261961722488	24.77197966507177	31.78453947368421
41	20.36044812469557	16.444977331484882	24.11105698977107	39.083517554048484
42	21.562291063071093	27.022509471807442	23.430651511774755	27.984547953346706
43	19.357235742151214	24.919190042727106	27.285900055731005	28.437674159390674
44	20.352862353904563	26.606119258542392	27.33566589741681	25.705352490136228
45	26.008535906476155	23.04323622193357	28.743737242531083	22.204490629059194
46	21.645922348133013	26.984952628645736	23.804569942411295	27.564555080809956
47	18.716477949940405	24.310935637663885	22.266090584028607	34.7064958283671
48	26.74552263710186	24.32051614817012	22.96711038599874	25.96685082872928
49	20.979904164035514	25.68626722632889	22.447160209501877	30.886668400133725
50	19.83970910170309	28.874624318207115	23.89892768357389	27.3867388965159
51	20.290555101434197	29.824626588392654	23.422753957048375	26.46206435312477
52	21.760427489980707	33.46445005195191	23.738310820840137	21.036811637227252
53	21.389580006684245	33.502915072969664	23.431987819822496	21.6755171005236
54	20.56727056727057	26.314226314226314	28.2001782001782	24.91832491832492
55	21.776110533353144	23.005496954390132	29.620413014410936	25.59797949784579
56	20.747011212593748	23.59842578154006	30.33340758892107	25.321155416945125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	1.0
7	1.0
8	1.5
9	2.0
10	16.0
11	30.0
12	51.0
13	87.5
14	103.0
15	100.0
16	97.0
17	97.5
18	98.0
19	102.0
20	106.0
21	96.0
22	89.0
23	92.0
24	105.5
25	119.0
26	123.5
27	128.0
28	145.5
29	172.5
30	182.0
31	199.5
32	217.0
33	253.0
34	289.0
35	344.0
36	399.0
37	418.0
38	439.5
39	442.0
40	472.5
41	503.0
42	601.0
43	699.0
44	907.5
45	1116.0
46	1540.5
47	2097.5
48	2230.0
49	1946.0
50	1662.0
51	1563.5
52	1465.0
53	1482.0
54	1505.0
55	1511.0
56	1490.0
57	1469.0
58	1557.5
59	1646.0
60	1690.0
61	1734.0
62	1698.0
63	1431.0
64	1200.0
65	1103.0
66	1006.0
67	905.5
68	805.0
69	755.0
70	705.0
71	582.5
72	359.0
73	258.0
74	212.5
75	167.0
76	125.0
77	83.0
78	57.5
79	33.5
80	35.0
81	21.0
82	7.0
83	6.5
84	6.0
85	4.5
86	3.0
87	1.5
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4667876856962916
16	0.06668395509947024
17	0.011113992516578373
18	0.0
19	0.037046641721927905
20	0.15559589523209721
21	0.0
22	0.0
23	0.0
24	0.0
25	0.007409328344385581
26	0.037046641721927905
27	0.05556996258289186
28	0.0
29	0.15189123105990443
30	0.037046641721927905
31	0.018523320860963952
32	0.014818656688771163
33	0.09632126847701256
34	0.15559589523209721
35	0.12966324602674767
36	0.07038861927166302
37	0.1222539176823621
38	0.18152854443744673
39	0.13707257437113324
40	0.8928240654984626
41	1.1262179083466084
42	0.2630311562256881
43	0.28896380543103767
44	0.4704923498684844
45	0.17782388026525398
46	0.28896380543103767
47	0.5371763049679547
48	0.08891194013262699
49	0.26673582039788096
50	0.15559589523209721
51	0.2926684696032305
52	0.1667098877486756
53	0.2370985070203386
54	0.21116585781498906
55	0.25562182788130255
56	0.21857518615937466
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	26993.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.36079724372985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36883440283498	69.13644278146187
2	6.584618331577436	10.187826473530174
3	1.848481946173738	4.290001111399252
4	0.8619863997701369	2.6673582039788095
5	0.38789387989656166	1.5003889897380802
6	0.2059189732784216	0.95580335642574
7	0.13887558662963317	0.7520468269551366
8	0.11972033330140792	0.7409328344385582
9	0.09098745330907002	0.6334975734449672
>10	0.3495833732401111	4.782721446300893
>50	0.03352169332439421	1.8671507427851664
>100	0.009577626664112633	2.4858296595413627
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	386	1.4300003704664173	No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	285	1.0558292890749454	No Hit
GACACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	92	0.3408291038417367	No Hit
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	89	0.3297151113251584	No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	89	0.3297151113251584	No Hit
GACACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	63	0.2333938428481458	No Hit
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	59	0.21857518615937466	No Hit
GACACTGGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC	59	0.21857518615937466	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	53	0.1963472011262179	No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	48	0.17782388026525398	No Hit
GACACTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	39	0.14448190271551883	No Hit
GACACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	38	0.14077723854332605	No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	35	0.12966324602674767	No Hit
GACACTGGGACTTGAAACTAGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGC	32	0.1185492535101693	No Hit
GACACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	31	0.1148445893379765	No Hit
GACACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	31	0.1148445893379765	No Hit
GACACTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC	31	0.1148445893379765	No Hit
GACACTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA	29	0.10743526099359092	No Hit
GACACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	29	0.10743526099359092	No Hit
GACACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT	28	0.10373059682139812	No Hit
GACACTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG	27	0.10002593264920535	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0037046641721927907	0.0	0.0	0.0	0.0
12	0.011113992516578372	0.0	0.0	0.0	0.0
13	0.011113992516578372	0.0	0.0	0.0	0.0
14	0.025932649205349535	0.0	0.0	0.0	0.0
15	0.04445597006631349	0.0	0.0	0.0	0.0
16	0.04445597006631349	0.0	0.0	0.0	0.0
17	0.04816063423850628	0.0	0.0	0.0	0.0
18	0.05186529841069907	0.0	0.0	0.0	0.0
19	0.05556996258289186	0.0	0.0	0.0	0.0
20	0.05556996258289186	0.0	0.0	0.0	0.0
21	0.05556996258289186	0.0	0.0	0.0	0.0
22	0.05556996258289186	0.0	0.0	0.0	0.0
23	0.05927462675508465	0.0	0.0	0.0	0.0
24	0.06297929092727744	0.0	0.0	0.0	0.0
25	0.06297929092727744	0.0	0.0	0.0	0.0
26	0.07409328344385581	0.0	0.0	0.0	0.0
27	0.08520727596043419	0.0	0.0	0.0	0.0
28	0.09261660430481977	0.0	0.0	0.0	0.0
29	0.09261660430481977	0.0	0.0	0.0	0.0
30	0.10002593264920535	0.0	0.0	0.0	0.0
31	0.12225391768236209	0.0	0.0	0.0	0.0
32	0.12225391768236209	0.0	0.0	0.0	0.0
33	0.12225391768236209	0.0	0.0	0.0	0.0
34	0.12225391768236209	0.0	0.0	0.0	0.0
35	0.12225391768236209	0.0	0.0	0.0	0.0
36	0.12966324602674767	0.0	0.0	0.0	0.0
37	0.12966324602674767	0.0	0.0	0.0	0.0
38	0.13336791019894045	0.0	0.0	0.0	0.0
39	0.13336791019894045	0.0	0.0	0.0	0.0
40	0.13336791019894045	0.0	0.0	0.0	0.0
41	0.13336791019894045	0.0	0.0	0.0	0.0
42	0.14077723854332605	0.0	0.0	0.0	0.0
43	0.14448190271551883	0.0	0.0	0.0	0.0
44	0.15189123105990443	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCC	20	3.7541776E-4	50.923954	41
TGTCCAA	20	3.7541776E-4	50.923954	38
ACCGCGG	15	0.00775795	50.923954	41
GTCCAAT	20	3.7541776E-4	50.923954	39
TCCAATC	20	3.7541776E-4	50.923954	40
GCATTGA	15	0.00775795	50.923954	40
TTTTGTC	30	9.0107096E-7	50.827324	35
CGGCCAA	25	1.9722827E-5	50.255157	45
CGCGGCC	25	1.9943765E-5	50.161045	43
TGGTCTG	15	0.008297616	50.06729	50
GACCGCA	35	5.0549716E-8	49.973885	12
AGACCGC	35	5.0549716E-8	49.973885	11
CATCCTC	20	4.121217E-4	49.97388	15
GCTCTTT	20	4.121217E-4	49.97388	10
ACCGCAC	30	1.0133663E-6	49.973877	13
CCACAGA	15	0.008359256	49.973877	10
GACTTAA	15	0.008359256	49.973877	10
GTACCTG	35	5.1300958E-8	49.88082	9
GGACTTA	15	0.008421237	49.880817	9
GGGCTAA	15	0.008421237	49.880817	9
>>END_MODULE