FastQCFastQC Report
Fri 17 Jun 2016
SRR1526073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGATGAGATGGACTTAAAGGAATTGACGGGTA60.33707865168539325No Hit
GACACTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA40.22471910112359553No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.22471910112359553No Hit
GACACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.16853932584269662No Hit
GACACTGGGTAGAGATGTGGAGGAATACCGGGTACGCCCC30.16853932584269662No Hit
GACACTGGGCTCAGTTTCTTCTTGGAGCATTGGGAAAATG30.16853932584269662No Hit
GACACTGGGAGCTGGTATCAACGCAGAGTCACTTTTTTTC30.16853932584269662No Hit
GACACTGGGAGCCAAGAACTCTTCCGTTCTTCCCTCTCCT20.11235955056179776No Hit
GACACTGGGATGAGATGGACTTAAATGAATTGACGGGTAC20.11235955056179776No Hit
GACACTGGGGGTTTCTTTTGGTTTCCTTTTGTTTTGTCCC20.11235955056179776No Hit
GACACTGGGGCCGAATAGCCTCTCCACCCAAGCCGCCCGC20.11235955056179776No Hit
GACACTGGGCACAGAGGTCCTCAAGACCAACGGACTCCTG20.11235955056179776No Hit
GACACTGGGATTTTGACCAAAGTTCTCTATTTTTCCTTGA20.11235955056179776No Hit
GACACTGGTCGCAACGTTTTTTTTTTTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGCTTGTGCCAAACCCTGGATGTTCTATGTTGT20.11235955056179776No Hit
GACACTGGGATGAGATGGACTCAAAGGAATTGACGGGTAC20.11235955056179776No Hit
GACACTGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA20.11235955056179776No Hit
GACACTGGGCTGGCACGAGAGTCGATTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGTATCACCAGAGTCGACTTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGACAGTCCGAGAGTCGCTTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGGAAGCATGGCACGCAGAGCGCCTTTTTTTTT20.11235955056179776No Hit
GACACTGGGCAACTTCTGGAAGAGGAGAATAAAGAGCGTG20.11235955056179776No Hit
GACACTGGGATGAGATGGACTTAAAGGAATTGACGGGTAC20.11235955056179776No Hit
GACACTGGGAATGTACGAAGCGATTTTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGAAGCAGGGATGAGATGGACTTAAAGGAATTG20.11235955056179776No Hit
GACACTGGGGATGAGATGGACTTAAATGAATTGACGGGTA20.11235955056179776No Hit
GACACTGGGGCCGAATAGCCTCTCCCCCCCAGCGGCCCGC20.11235955056179776No Hit
GACACTGGGGAGTCGACTCTTTTTTTTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGTTCCGAGCGTTTTTTTTTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGCCATCGACTTTTTTTTTTTTTTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGAACTTAAGTTTGTGAGTGTATCCTTTCTAAA20.11235955056179776No Hit
GACACTGGGGATCCAGTGACAGATACCATTAGATTCAAGA20.11235955056179776No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC20.11235955056179776No Hit
GACACTGGGAGCTGGCAACGCAGAGTCGACTTTTTTTTTT20.11235955056179776No Hit
GACACTGGGGCTCTCTTGCTTTTCTCTCTCTCTTGCTTCG20.11235955056179776No Hit
GACACTGGGCAGTTTCAACGCAGAGTCGACTTTTGTAATT20.11235955056179776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGA308.064844E-634.05
CTGGGCA200.002098324734.05
CTGGGAG200.002098324734.05
CTGGGAA251.318037E-434.05
ACTGGGC451.549779E-934.04
ACTGGGA600.034.04
TGGGAAG200.002098324734.06
ACACTGG1800.033.0555532
GACACTG1800.033.0555531
CACTGGG1750.033.0285723
ACTGGGG552.3464963E-1030.909094
Produced by FastQC (version 0.11.5)