##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526073_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1780
Sequences flagged as poor quality	0
Sequence length	40
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.730898876404495	30.0	26.0	33.0	4.0	35.0
2	19.217977528089886	21.0	11.0	27.0	4.0	32.0
3	19.18258426966292	20.0	10.0	28.0	4.0	33.0
4	22.06123595505618	25.0	15.0	31.0	4.0	34.0
5	13.978089887640449	12.0	10.0	15.0	4.0	29.0
6	22.40449438202247	25.0	15.0	31.0	4.0	35.0
7	22.25	26.0	13.0	31.0	4.0	34.0
8	23.11179775280899	27.0	15.0	32.0	4.0	35.0
9	21.880898876404494	25.0	12.0	31.0	4.0	34.0
10	18.6438202247191	19.0	9.0	28.0	4.0	33.0
11	23.07191011235955	27.0	11.0	34.0	4.0	37.0
12	22.956741573033707	27.0	11.0	34.0	4.0	37.0
13	23.484831460674158	28.0	10.0	34.0	4.0	37.0
14	23.447752808988763	28.0	10.0	35.0	4.0	37.0
15	22.12696629213483	26.0	4.0	34.0	4.0	37.0
16	18.991011235955057	20.0	4.0	31.0	4.0	35.0
17	20.031460674157305	24.0	4.0	32.0	4.0	35.0
18	19.886516853932584	23.0	4.0	32.0	4.0	35.0
19	17.440449438202247	17.0	4.0	30.0	4.0	34.0
20	18.594382022471912	20.0	4.0	31.0	4.0	35.0
21	16.14943820224719	14.0	4.0	28.0	4.0	33.0
22	16.31123595505618	13.0	4.0	29.0	4.0	33.0
23	16.529775280898875	15.0	4.0	30.0	4.0	33.0
24	15.87191011235955	12.0	4.0	29.0	4.0	33.0
25	15.512359550561797	4.0	4.0	29.0	4.0	33.0
26	13.139887640449437	4.0	4.0	24.0	4.0	32.0
27	13.116292134831461	4.0	4.0	25.0	4.0	33.0
28	12.882022471910112	4.0	4.0	25.0	4.0	33.0
29	12.49494382022472	4.0	4.0	24.0	4.0	32.0
30	11.805056179775281	4.0	4.0	21.0	4.0	31.0
31	11.290449438202247	4.0	4.0	20.0	4.0	32.0
32	10.535393258426966	4.0	4.0	16.0	4.0	31.0
33	10.17808988764045	4.0	4.0	14.0	4.0	31.0
34	9.713483146067416	4.0	4.0	11.0	4.0	30.0
35	9.12752808988764	4.0	4.0	4.0	4.0	29.0
36	9.215730337078652	4.0	4.0	4.0	4.0	30.0
37	9.141011235955057	4.0	4.0	4.0	4.0	31.0
38	9.067977528089887	4.0	4.0	4.0	4.0	31.0
39	8.69438202247191	4.0	4.0	4.0	4.0	30.0
40	8.369101123595506	4.0	4.0	4.0	4.0	29.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	2.9626506024096386
1	2	9.42751004016064
1	3	-8.56144578313253
1	4	2.5058232931726927
1	5	6.133333333333333
1	6	5.927911646586342
1	7	7.287148594377509
1	8	-3.621084337349398
1	9	-11.484738955823293
1	10	1.1819277108433717
1	11	-2.15602409638554
1	12	0.9303212851405611
1	13	-9.13734939759036
1	14	-5.056827309236947
1	15	-6.153012048192771
1	16	1.6265060240963898
1	17	-12.588955823293173
1	18	-7.856626506024096
1	19	-5.341967871485945
1	20	-3.7670682730923684
1	21	3.452409638554217
1	22	2.661044176706829
1	23	0.7016064257028081
1	24	0.8483935742971891
1	25	3.188152610441769
1	26	6.1688755020080315
1	27	5.172088353413654
1	28	5.583534136546184
1	29	9.99859437751004
1	30	10.544979919678715
1	31	6.544779116465863
1	32	4.965461847389557
1	33	2.930321285140563
1	34	1.4319277108433717
1	35	2.3616465863453815
1	36	1.7726907630522097
1	37	10.917269076305221
1	38	1.4622489959839378
1	39	10.58433734939759
1	40	2.4248995983935737
6	1	1.5459839357429708
6	2	-0.9058232931726913
6	3	0.3552208835341375
6	4	-3.9941767068273073
6	5	-2.450000000000001
6	6	4.927911646586342
6	7	-2.296184738955823
6	8	-3.871084337349398
6	9	1.4319277108433752
6	10	0.01526104417670382
6	11	-0.9060240963855399
6	12	6.763654618473893
6	13	-1.9706827309236914
6	14	-4.056827309236947
6	15	9.513654618473897
6	16	1.959839357429722
6	17	9.161044176706827
6	18	3.4767068273092363
6	19	-4.258634538152611
6	20	-4.767068273092368
6	21	7.619076305220885
6	22	-1.4222891566265048
6	23	3.451606425702808
6	24	7.598393574297189
6	25	5.771485943775101
6	26	11.002208835341364
6	27	9.838755020080322
6	28	12.000200803212852
6	29	7.415261044176708
6	30	13.128313253012047
6	31	12.46144578313253
6	32	10.132128514056225
6	33	10.013654618473895
6	34	12.765261044176704
6	35	6.3616465863453815
6	36	4.939357429718876
6	37	5.833935742971888
6	38	5.628915662650604
6	39	-2.0823293172690764
6	40	5.59156626506024
7	1	0.8793172690763065
7	2	6.260843373493973
7	3	6.18855421686747
7	4	5.005823293172693
7	5	1.049999999999999
7	6	5.261244979919674
7	7	5.703815261044177
7	8	3.462248995983934
7	9	4.431927710843375
7	10	9.84859437751004
7	11	5.260642570281128
7	12	1.596987951807229
7	13	5.195983935742973
7	14	3.6098393574297205
7	15	6.513654618473897
7	16	5.293172690763058
7	17	3.827710843373495
7	18	2.643373493975904
7	19	8.741365461847387
7	20	0.23293172690763164
7	21	-3.880923694779117
7	22	-0.7556224899598369
7	23	-4.715060240963858
7	24	-2.901606425702811
7	25	-2.2285140562248973
7	26	-1.1644578313253025
7	27	-0.16124497991967957
7	28	-3.4997991967871487
7	29	-0.08473895582329405
7	30	-0.03835341365461886
7	31	-5.205220883534137
7	32	-4.534538152610443
7	33	-4.319678714859437
7	34	-4.568072289156628
7	35	-3.6383534136546185
7	36	-3.7273092369477903
7	37	-3.832730923694778
7	38	-4.037751004016062
7	39	-3.4156626506024095
7	40	-3.0751004016064263
10	1	4.545983935742971
10	2	0.42751004016064087
10	3	0.8552208835341375
10	4	11.339156626506025
10	5	0.21666666666666679
10	6	9.427911646586342
10	7	9.370481927710841
10	8	10.128915662650602
10	9	9.59859437751004
10	10	7.681927710843372
10	11	13.260642570281124
10	12	9.263654618473893
10	13	8.86265060240964
10	14	11.109839357429717
10	15	7.346987951807229
10	16	13.959839357429722
10	17	10.99437751004016
10	18	17.143373493975904
10	19	16.908032128514055
10	20	17.899598393574294
10	21	14.452409638554217
10	22	23.077710843373495
10	23	18.784939759036142
10	24	18.765060240963855
10	25	15.771485943775101
10	26	13.502208835341364
10	27	15.005421686746987
10	28	18.666867469879517
10	29	15.581927710843372
10	30	11.794979919678715
10	31	17.461445783132532
10	32	11.632128514056225
10	33	14.84698795180723
10	34	14.265261044176704
10	35	13.02831325301205
10	36	7.606024096385544
10	37	6.500602409638556
10	38	5.128915662650604
10	39	9.417670682730922
10	40	8.258232931726909
13	1	10.712650602409639
13	2	6.427510040160641
13	3	5.68855421686747
13	4	5.005823293172693
13	5	12.883333333333333
13	6	6.427911646586342
13	7	5.037148594377509
13	8	2.628915662650602
13	9	3.2652610441767074
13	10	18.18192771084337
13	11	14.09397590361446
13	12	10.430321285140561
13	13	2.3626506024096408
13	14	10.943172690763053
13	15	12.846987951807229
13	16	4.12650602409639
13	17	3.6610441767068274
13	18	15.643373493975904
13	19	6.408032128514055
13	20	8.732931726907632
13	21	18.452409638554215
13	22	17.41104417670683
13	23	19.451606425702806
13	24	18.09839357429719
13	25	22.93815261044177
13	26	9.668875502008031
13	27	10.672088353413654
13	28	7.833534136546184
13	29	10.74859437751004
13	30	7.794979919678715
13	31	-5.205220883534137
13	32	-4.534538152610443
13	33	-4.319678714859437
13	34	-4.568072289156628
13	35	-3.6383534136546185
13	36	-3.7273092369477903
13	37	-3.832730923694778
13	38	-4.037751004016062
13	39	-3.4156626506024095
13	40	-3.0751004016064263
14	1	11.212650602409639
14	2	18.42751004016064
14	3	10.68855421686747
14	4	12.005823293172693
14	5	6.883333333333333
14	6	12.927911646586342
14	7	14.03714859437751
14	8	13.128915662650602
14	9	15.765261044176707
14	10	1.6819277108433717
14	11	5.09397590361446
14	12	-4.569678714859439
14	13	10.36265060240964
14	14	2.9431726907630527
14	15	11.846987951807229
14	16	15.62650602409639
14	17	16.161044176706827
14	18	15.143373493975904
14	19	19.408032128514055
14	20	19.23293172690763
14	21	11.952409638554217
14	22	-2.088955823293171
14	23	18.451606425702806
14	24	13.598393574297189
14	25	1.4381526104417688
14	26	19.66887550200803
14	27	-5.827911646586346
14	28	-5.666465863453816
14	29	-5.25140562248996
14	30	-5.205020080321285
14	31	-5.205220883534137
14	32	-4.534538152610443
14	33	-4.319678714859437
14	34	-4.568072289156628
14	35	-3.6383534136546185
14	36	-3.7273092369477903
14	37	-3.832730923694778
14	38	-4.037751004016062
14	39	-3.4156626506024095
14	40	-3.0751004016064263
16	1	3.2126506024096386
16	2	4.427510040160641
16	3	2.6885542168674696
16	4	7.005823293172693
16	5	-1.1166666666666671
16	6	-8.072088353413658
16	7	-1.9628514056224908
16	8	4.128915662650602
16	9	6.765261044176707
16	10	9.681927710843372
16	11	16.09397590361446
16	12	16.43032128514056
16	13	16.36265060240964
16	14	15.943172690763053
16	15	17.84698795180723
16	16	6.62650602409639
16	17	17.161044176706827
16	18	20.143373493975904
16	19	22.408032128514055
16	20	22.23293172690763
16	21	20.952409638554215
16	22	1.9110441767068291
16	23	20.451606425702806
16	24	11.598393574297189
16	25	22.43815261044177
16	26	-6.3311244979919685
16	27	-5.827911646586346
16	28	-5.666465863453816
16	29	-5.25140562248996
16	30	-5.205020080321285
16	31	-5.205220883534137
16	32	-4.534538152610443
16	33	-4.319678714859437
16	34	-4.568072289156628
16	35	-3.6383534136546185
16	36	-3.7273092369477903
16	37	-3.832730923694778
16	38	-4.037751004016062
16	39	-3.4156626506024095
16	40	-3.0751004016064263
17	1	-0.6206827309236935
17	2	1.4275100401606409
17	3	4.521887550200802
17	4	2.0058232931726927
17	5	-1.450000000000001
17	6	4.59457831325301
17	7	5.037148594377509
17	8	2.462248995983934
17	9	4.0985943775100395
17	10	0.18192771084337167
17	11	5.260642570281128
17	12	7.430321285140561
17	13	3.8626506024096408
17	14	3.4431726907630527
17	15	7.346987951807229
17	16	1.2931726907630576
17	17	3.6610441767068274
17	18	-0.5232931726907637
17	19	3.0746987951807228
17	20	0.5662650602409638
17	21	-1.7142570281124492
17	22	3.7443775100401613
17	23	1.284939759036142
17	24	0.431726907630523
17	25	1.2714859437751027
17	26	0.0022088353413654005
17	27	6.005421686746988
17	28	3.8335341365461844
17	29	4.24859437751004
17	30	2.961646586345381
17	31	7.794779116465863
17	32	0.13212851405622317
17	33	3.180321285140563
17	34	1.7652610441767056
17	35	2.6949799196787154
17	36	-1.8939759036144572
17	37	1.3339357429718879
17	38	0.6289156626506038
17	39	1.7510040160642566
17	40	2.09156626506024
18	1	8.879317269076303
18	2	13.260843373493973
18	3	9.021887550200802
18	4	12.005823293172693
18	5	16.383333333333333
18	6	13.094578313253006
18	7	12.53714859437751
18	8	14.462248995983938
18	9	9.931927710843375
18	10	13.84859437751004
18	11	6.927309236947792
18	12	6.763654618473893
18	13	9.36265060240964
18	14	6.943172690763053
18	15	11.680321285140561
18	16	8.459839357429722
18	17	8.827710843373495
18	18	10.310040160642572
18	19	13.574698795180723
18	20	8.066265060240964
18	21	10.452409638554217
18	22	16.74437751004016
18	23	13.78493975903614
18	24	16.765060240963855
18	25	9.271485943775101
18	26	4.002208835341365
18	27	8.838755020080322
18	28	8.666867469879517
18	29	8.581927710843372
18	30	8.628313253012047
18	31	8.294779116465863
18	32	9.132128514056225
18	33	7.846987951807231
18	34	3.0985943775100377
18	35	8.361646586345381
18	36	8.439357429718878
18	37	7.500602409638556
18	38	10.29558232931727
18	39	8.751004016064257
18	40	9.424899598393573
19	1	-0.2873493975903614
19	2	-5.572489959839359
19	3	-3.8114457831325304
19	4	-0.4941767068273073
19	5	-1.1166666666666671
19	6	2.9279116465863417
19	7	-0.9628514056224908
19	8	-0.37108433734939794
19	9	-1.7347389558232926
19	10	6.181927710843372
19	11	15.09397590361446
19	12	7.430321285140561
19	13	13.86265060240964
19	14	5.443172690763053
19	15	15.846987951807229
19	16	8.12650602409639
19	17	1.6610441767068274
19	18	7.143373493975904
19	19	7.908032128514055
19	20	13.732931726907632
19	21	-0.0475903614457831
19	22	-2.088955823293171
19	23	-1.048393574297192
19	24	2.598393574297189
19	25	4.438152610441769
19	26	-6.3311244979919685
19	27	-5.827911646586346
19	28	-5.666465863453816
19	29	-5.25140562248996
19	30	-5.205020080321285
19	31	-5.205220883534137
19	32	-4.534538152610443
19	33	-4.319678714859437
19	34	-4.568072289156628
19	35	-3.6383534136546185
19	36	-3.7273092369477903
19	37	-3.832730923694778
19	38	-4.037751004016062
19	39	-3.4156626506024095
19	40	-3.0751004016064263
22	1	0.5459839357429708
22	2	1.4275100401606409
22	3	-6.31144578313253
22	4	-3.660843373493975
22	5	-1.450000000000001
22	6	7.261244979919674
22	7	8.03714859437751
22	8	6.462248995983934
22	9	8.09859437751004
22	10	-2.6514056224899623
22	11	12.760642570281124
22	12	11.430321285140561
22	13	10.029317269076309
22	14	5.943172690763053
22	15	15.180321285140565
22	16	13.959839357429722
22	17	12.827710843373495
22	18	11.476706827309236
22	19	8.741365461847387
22	20	11.8995983935743
22	21	14.619076305220885
22	22	11.244377510040161
22	23	11.78493975903614
22	24	14.931726907630521
22	25	14.438152610441769
22	26	11.3355421686747
22	27	5.505421686746988
22	28	13.000200803212852
22	29	6.74859437751004
22	30	6.794979919678715
22	31	11.128112449799195
22	32	12.79879518072289
22	33	15.680321285140563
22	34	5.765261044176706
22	35	6.694979919678715
22	36	3.6060240963855437
22	37	-1.4993975903614452
22	38	2.9622489959839378
22	39	-0.4156626506024095
22	40	6.59156626506024
24	1	5.81265060240964
24	2	-1.9724899598393595
24	3	-3.7114457831325307
24	4	9.605823293172694
24	5	-0.5166666666666675
24	6	9.32791164658634
24	7	8.03714859437751
24	8	6.928915662650603
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106	4	8.505823293172693
106	5	-0.11666666666666714
106	6	8.927911646586342
106	7	2.537148594377509
106	8	8.128915662650602
106	9	8.765261044176707
106	10	-6.818072289156628
106	11	8.59397590361446
106	12	14.930321285140561
106	13	7.862650602409641
106	14	13.943172690763053
106	15	7.846987951807229
106	16	20.12650602409639
106	17	17.161044176706827
106	18	17.143373493975904
106	19	8.908032128514055
106	20	11.732931726907632
106	21	9.952409638554217
106	22	2.911044176706829
106	23	9.451606425702808
106	24	11.598393574297189
106	25	-0.5618473895582312
106	26	-2.8311244979919685
106	27	-0.32791164658634564
106	28	7.333534136546184
106	29	-5.25140562248996
106	30	-5.205020080321285
106	31	-5.205220883534137
106	32	-4.534538152610443
106	33	-4.319678714859437
106	34	-4.568072289156628
106	35	-3.6383534136546185
106	36	-3.7273092369477903
106	37	-3.832730923694778
106	38	-4.037751004016062
106	39	-3.4156626506024095
106	40	-3.0751004016064263
107	1	-21.78734939759036
107	2	-13.57248995983936
107	3	-14.31144578313253
107	4	-15.994176706827307
107	5	-9.116666666666667
107	6	-16.07208835341366
107	7	-16.96285140562249
107	8	-17.871084337349398
107	9	-16.234738955823293
107	10	-13.318072289156628
107	11	-16.90602409638554
107	12	-17.56967871485944
107	13	-17.63734939759036
107	14	-18.056827309236947
107	15	-16.15301204819277
107	16	-13.37349397590361
107	17	-14.838955823293173
107	18	-13.856626506024096
107	19	-11.591967871485945
107	20	-11.767068273092368
107	21	-10.047590361445783
107	22	-9.08895582329317
107	23	-10.548393574297192
107	24	-9.401606425702811
107	25	-8.561847389558231
107	26	-6.3311244979919685
107	27	-5.827911646586346
107	28	-5.666465863453816
107	29	-5.25140562248996
107	30	-5.205020080321285
107	31	-5.205220883534137
107	32	-4.534538152610443
107	33	-4.319678714859437
107	34	-4.568072289156628
107	35	-3.6383534136546185
107	36	-3.7273092369477903
107	37	-3.832730923694778
107	38	-4.037751004016062
107	39	-3.4156626506024095
107	40	-3.0751004016064263
108	1	3.2126506024096386
108	2	3.427510040160641
108	3	4.68855421686747
108	4	-5.994176706827307
108	5	-1.1166666666666671
108	6	-1.0720883534136583
108	7	5.037148594377509
108	8	8.128915662650602
108	9	8.765261044176707
108	10	-5.318072289156628
108	11	14.09397590361446
108	12	3.430321285140561
108	13	13.36265060240964
108	14	10.943172690763053
108	15	-16.15301204819277
108	16	-13.37349397590361
108	17	-14.838955823293173
108	18	-13.856626506024096
108	19	-11.591967871485945
108	20	-11.767068273092368
108	21	-10.047590361445783
108	22	-9.08895582329317
108	23	-10.548393574297192
108	24	-9.401606425702811
108	25	-8.561847389558231
108	26	-6.3311244979919685
108	27	-5.827911646586346
108	28	-5.666465863453816
108	29	-5.25140562248996
108	30	-5.205020080321285
108	31	-5.205220883534137
108	32	-4.534538152610443
108	33	-4.319678714859437
108	34	-4.568072289156628
108	35	-3.6383534136546185
108	36	-3.7273092369477903
108	37	-3.832730923694778
108	38	-4.037751004016062
108	39	-3.4156626506024095
108	40	-3.0751004016064263
112	1	-14.454016064257027
112	2	-11.57248995983936
112	3	-10.31144578313253
112	4	-9.660843373493973
112	5	-6.45
112	6	-9.072088353413658
112	7	-12.296184738955825
112	8	-11.204417670682732
112	9	-10.234738955823293
112	10	-9.318072289156628
112	11	-6.239357429718874
112	12	-6.903012048192773
112	13	-12.304016064257025
112	14	-7.056827309236947
112	15	-5.486345381526105
112	16	-4.040160642570276
112	17	-10.172289156626507
112	18	-5.523293172690762
112	19	-2.925301204819279
112	20	-3.7670682730923684
112	21	-1.7142570281124492
112	22	-7.088955823293171
112	23	-6.548393574297192
112	24	-0.7349397590361448
112	25	-8.561847389558231
112	26	-6.3311244979919685
112	27	-5.827911646586346
112	28	-5.666465863453816
112	29	-5.25140562248996
112	30	-5.205020080321285
112	31	-5.205220883534137
112	32	-4.534538152610443
112	33	-4.319678714859437
112	34	-4.568072289156628
112	35	-3.6383534136546185
112	36	-3.7273092369477903
112	37	-3.832730923694778
112	38	-4.037751004016062
112	39	-3.4156626506024095
112	40	-3.0751004016064263
114	1	4.212650602409639
114	2	11.42751004016064
114	3	-5.31144578313253
114	4	-6.994176706827307
114	5	-6.116666666666667
114	6	10.927911646586342
114	7	7.037148594377509
114	8	6.128915662650602
114	9	4.765261044176707
114	10	10.681927710843372
114	11	18.09397590361446
114	12	12.430321285140561
114	13	17.36265060240964
114	14	16.943172690763053
114	15	18.84698795180723
114	16	19.62650602409639
114	17	19.161044176706827
114	18	20.143373493975904
114	19	11.408032128514055
114	20	22.23293172690763
114	21	2.952409638554217
114	22	19.91104417670683
114	23	18.451606425702806
114	24	-9.401606425702811
114	25	-8.561847389558231
114	26	-6.3311244979919685
114	27	-5.827911646586346
114	28	-5.666465863453816
114	29	-5.25140562248996
114	30	-5.205020080321285
114	31	-5.205220883534137
114	32	-4.534538152610443
114	33	-4.319678714859437
114	34	-4.568072289156628
114	35	-3.6383534136546185
114	36	-3.7273092369477903
114	37	-3.832730923694778
114	38	-4.037751004016062
114	39	-3.4156626506024095
114	40	-3.0751004016064263
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	312.0
5	20.0
6	12.0
7	14.0
8	11.0
9	25.0
10	46.0
11	62.0
12	69.0
13	78.0
14	57.0
15	80.0
16	86.0
17	74.0
18	72.0
19	79.0
20	83.0
21	79.0
22	88.0
23	92.0
24	94.0
25	64.0
26	58.0
27	42.0
28	30.0
29	18.0
30	21.0
31	9.0
32	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.15682967959528	0.7869589657110737	0.0	0.056211354693648116
8	98.03260258572232	0.6183249016301293	0.39347948285553686	0.9555930297920181
9	96.51489600899382	1.967397414277684	0.6745362563237773	0.8431703204047217
10	27.76840921866217	42.10230466554244	9.83698707138842	20.29229904440697
11	23.98876404494382	30.39325842696629	27.303370786516858	18.314606741573034
12	32.078651685393254	16.51685393258427	26.741573033707866	24.662921348314608
13	26.40449438202247	19.9438202247191	28.146067415730336	25.50561797752809
14	20.674157303370784	27.921348314606742	27.52808988764045	23.876404494382022
15	22.696629213483146	28.258426966292134	28.539325842696627	20.50561797752809
16	25.337078651685392	26.46067415730337	25.056179775280903	23.146067415730336
17	23.314606741573034	25.168539325842698	26.573033707865168	24.9438202247191
18	26.741573033707866	26.741573033707866	22.359550561797754	24.15730337078652
19	20.631341600901916	17.531003382187148	20.180383314543406	41.65727170236753
20	22.58426966292135	27.247191011235955	24.49438202247191	25.674157303370787
21	22.359550561797754	21.910112359550563	24.719101123595504	31.011235955056183
22	21.910112359550563	19.9438202247191	26.62921348314607	31.516853932584272
23	22.134831460674157	25.89887640449438	29.382022471910112	22.58426966292135
24	19.8876404494382	25.0	24.831460674157306	30.280898876404493
25	21.585160202360875	29.00505902192243	23.66498032602586	25.744800449690835
26	19.063733784545967	26.84715172024817	26.22673434856176	27.862380146644107
27	22.528089887640448	21.40449438202247	29.213483146067414	26.853932584269664
28	18.662169758291174	23.88982574480045	29.848229342327148	27.599775154581224
29	17.8490990990991	24.774774774774773	32.0945945945946	25.28153153153153
30	15.804274465691789	19.797525309336333	37.570303712035994	26.827896512935883
31	15.596846846846846	22.52252252252252	39.189189189189186	22.691441441441444
32	18.14606741573034	18.314606741573034	36.741573033707866	26.79775280898876
33	16.51685393258427	19.04494382022472	40.50561797752809	23.93258426966292
34	14.662921348314606	20.44943820224719	42.07865168539326	22.808988764044944
35	14.55056179775281	19.49438202247191	40.337078651685395	25.617977528089884
36	15.056179775280897	20.1123595505618	40.78651685393259	24.044943820224717
37	15.691788526434195	18.7289088863892	40.21372328458943	25.365579302587175
38	15.730337078651685	19.9438202247191	39.60674157303371	24.719101123595504
39	13.876404494382022	20.0	41.34831460674157	24.775280898876403
40	13.988764044943819	24.662921348314608	40.39325842696629	20.95505617977528
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	2.5
17	3.0
18	3.0
19	5.0
20	7.0
21	11.0
22	15.0
23	15.0
24	20.5
25	26.0
26	23.0
27	20.0
28	20.0
29	24.5
30	29.0
31	42.0
32	55.0
33	55.0
34	58.5
35	62.0
36	78.0
37	94.0
38	94.0
39	108.0
40	122.0
41	117.5
42	113.0
43	113.0
44	120.5
45	128.0
46	141.0
47	154.0
48	154.0
49	134.0
50	114.0
51	120.5
52	127.0
53	127.0
54	121.0
55	115.0
56	111.0
57	107.0
58	107.0
59	114.5
60	122.0
61	114.0
62	106.0
63	106.0
64	96.0
65	86.0
66	71.5
67	57.0
68	57.0
69	53.5
70	50.0
71	36.0
72	22.0
73	22.0
74	24.0
75	26.0
76	19.0
77	12.0
78	12.0
79	8.5
80	5.0
81	3.0
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.05617977528089888
8	0.05617977528089888
9	0.05617977528089888
10	0.05617977528089888
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.33707865168539325
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.05617977528089888
26	0.39325842696629215
27	0.0
28	0.05617977528089888
29	0.22471910112359553
30	0.11235955056179776
31	0.22471910112359553
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.11235955056179776
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	1780.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.30337078651685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.92147806004618	95.28089887640449
2	1.674364896073903	3.258426966292135
3	0.23094688221709006	0.6741573033707865
4	0.11547344110854503	0.44943820224719105
5	0.0	0.0
6	0.057736720554272515	0.33707865168539325
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACACTGGGGATGAGATGGACTTAAAGGAATTGACGGGTA	6	0.33707865168539325	No Hit
GACACTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	4	0.22471910112359553	No Hit
GACACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	4	0.22471910112359553	No Hit
GACACTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	3	0.16853932584269662	No Hit
GACACTGGGTAGAGATGTGGAGGAATACCGGGTACGCCCC	3	0.16853932584269662	No Hit
GACACTGGGCTCAGTTTCTTCTTGGAGCATTGGGAAAATG	3	0.16853932584269662	No Hit
GACACTGGGAGCTGGTATCAACGCAGAGTCACTTTTTTTC	3	0.16853932584269662	No Hit
GACACTGGGAGCCAAGAACTCTTCCGTTCTTCCCTCTCCT	2	0.11235955056179776	No Hit
GACACTGGGATGAGATGGACTTAAATGAATTGACGGGTAC	2	0.11235955056179776	No Hit
GACACTGGGGGTTTCTTTTGGTTTCCTTTTGTTTTGTCCC	2	0.11235955056179776	No Hit
GACACTGGGGCCGAATAGCCTCTCCACCCAAGCCGCCCGC	2	0.11235955056179776	No Hit
GACACTGGGCACAGAGGTCCTCAAGACCAACGGACTCCTG	2	0.11235955056179776	No Hit
GACACTGGGATTTTGACCAAAGTTCTCTATTTTTCCTTGA	2	0.11235955056179776	No Hit
GACACTGGTCGCAACGTTTTTTTTTTTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGCTTGTGCCAAACCCTGGATGTTCTATGTTGT	2	0.11235955056179776	No Hit
GACACTGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	2	0.11235955056179776	No Hit
GACACTGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA	2	0.11235955056179776	No Hit
GACACTGGGCTGGCACGAGAGTCGATTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGTATCACCAGAGTCGACTTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGACAGTCCGAGAGTCGCTTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGGAAGCATGGCACGCAGAGCGCCTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGCAACTTCTGGAAGAGGAGAATAAAGAGCGTG	2	0.11235955056179776	No Hit
GACACTGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	2	0.11235955056179776	No Hit
GACACTGGGAATGTACGAAGCGATTTTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGAAGCAGGGATGAGATGGACTTAAAGGAATTG	2	0.11235955056179776	No Hit
GACACTGGGGATGAGATGGACTTAAATGAATTGACGGGTA	2	0.11235955056179776	No Hit
GACACTGGGGCCGAATAGCCTCTCCCCCCCAGCGGCCCGC	2	0.11235955056179776	No Hit
GACACTGGGGAGTCGACTCTTTTTTTTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGTTCCGAGCGTTTTTTTTTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGCCATCGACTTTTTTTTTTTTTTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGAACTTAAGTTTGTGAGTGTATCCTTTCTAAA	2	0.11235955056179776	No Hit
GACACTGGGGATCCAGTGACAGATACCATTAGATTCAAGA	2	0.11235955056179776	No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	2	0.11235955056179776	No Hit
GACACTGGGAGCTGGCAACGCAGAGTCGACTTTTTTTTTT	2	0.11235955056179776	No Hit
GACACTGGGGCTCTCTTGCTTTTCTCTCTCTCTTGCTTCG	2	0.11235955056179776	No Hit
GACACTGGGCAGTTTCAACGCAGAGTCGACTTTTGTAATT	2	0.11235955056179776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.056179775280898875	0.0	0.0	0.0	0.0
19	0.056179775280898875	0.0	0.0	0.0	0.0
20	0.056179775280898875	0.0	0.0	0.0	0.0
21	0.056179775280898875	0.0	0.0	0.0	0.0
22	0.056179775280898875	0.0	0.0	0.0	0.0
23	0.056179775280898875	0.0	0.0	0.0	0.0
24	0.056179775280898875	0.0	0.0	0.0	0.0
25	0.056179775280898875	0.0	0.0	0.0	0.0
26	0.056179775280898875	0.0	0.0	0.0	0.0
27	0.056179775280898875	0.0	0.0	0.0	0.0
28	0.056179775280898875	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGGGGA	30	8.064844E-6	34.0	5
CTGGGCA	20	0.0020983247	34.0	5
CTGGGAG	20	0.0020983247	34.0	5
CTGGGAA	25	1.318037E-4	34.0	5
ACTGGGC	45	1.549779E-9	34.0	4
ACTGGGA	60	0.0	34.0	4
TGGGAAG	20	0.0020983247	34.0	6
ACACTGG	180	0.0	33.055553	2
GACACTG	180	0.0	33.055553	1
CACTGGG	175	0.0	33.028572	3
ACTGGGG	55	2.3464963E-10	30.90909	4
>>END_MODULE