FastQCFastQC Report
Fri 17 Jun 2016
SRR1526070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110213
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG16621.5079890756988739No Hit
TGGATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT5140.4663696660103618No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3860.35023091649805377No Hit
TGGATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3800.3447869126146643No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2730.24770217669421937No Hit
TGGATGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2450.222296825238402No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2050.18600346601580575No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1890.17148612232676727No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1670.15152477475433931No Hit
TGGATGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1520.13791476504586572No Hit
TGGATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1360.12339742135682723No Hit
TGGATGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1350.12249008737626234No Hit
TGGATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1270.11523141553174308No Hit
TGGATGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1210.10978741164835364No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1150.10434340776496422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATA150.00807168150.52301841
CGCATTG203.9946323E-450.4301539
GCGCATT203.9946323E-450.4301538
ATATCAT204.0037066E-450.40698237
ACGCTTA150.008145425550.40698237
AATATCA150.008219670550.29147336
TCAGTCA150.008219670550.29147336
TAAGACC150.00823457950.2684435
TTATACT204.0861152E-450.19945543
ATTATAC204.0861152E-450.19945542
TATACTA204.0861152E-450.19945544
TAATCCG150.00827942850.1994542
GTTACAG150.00830942750.15356434
CAAACAT150.008339507550.10776514
TCGTGAT204.1324768E-450.08489612
ACTAATC204.1324768E-450.08489610
GACCCAC150.00836966850.0620469
GACGGAA150.00836966850.0620469
CGATTAA402.6157068E-950.06204249
GACTAAT204.141799E-450.0620429