##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526070_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 110213 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.103844374075656 39.0 38.0 40.0 33.0 40.0 2 36.45236042935044 39.0 36.0 40.0 30.0 40.0 3 36.203787212034875 39.0 36.0 40.0 29.0 40.0 4 34.219719996733595 38.0 35.0 39.0 17.0 40.0 5 36.461569869253175 39.0 37.0 40.0 30.0 40.0 6 36.30266846923684 39.0 36.0 40.0 30.0 40.0 7 36.670311124821936 39.0 37.0 40.0 31.0 40.0 8 36.41871648535109 39.0 37.0 40.0 31.0 40.0 9 36.47151424968016 39.0 37.0 40.0 31.0 40.0 10 35.551695353542684 39.0 35.0 40.0 27.0 40.0 11 34.892544436681696 38.0 34.0 39.0 26.0 40.0 12 34.04676399335832 38.0 32.0 39.0 22.0 40.0 13 33.70844637202508 38.0 32.0 39.0 20.0 40.0 14 34.25695698329598 38.0 33.0 39.0 23.0 40.0 15 33.19342545797683 38.0 32.0 39.0 18.0 40.0 16 32.700126119423295 37.0 30.0 39.0 19.0 40.0 17 31.77065318973261 36.0 28.0 39.0 15.0 40.0 18 31.092411965920537 35.0 26.0 39.0 14.0 40.0 19 32.255351002150384 36.0 29.0 39.0 18.0 40.0 20 32.91778646802101 37.0 30.0 39.0 21.0 40.0 21 33.02446172411603 38.0 31.0 39.0 19.0 40.0 22 32.30762251277073 37.0 29.0 39.0 16.0 40.0 23 32.97101975266076 38.0 31.0 39.0 19.0 40.0 24 32.395724642283575 37.0 30.0 39.0 17.0 40.0 25 32.79828150944081 37.0 30.0 39.0 19.0 40.0 26 33.26663823686861 38.0 32.0 39.0 19.0 40.0 27 33.51806048288314 38.0 33.0 39.0 20.0 40.0 28 33.48918911562157 38.0 33.0 39.0 21.0 40.0 29 32.975583642583 38.0 31.0 39.0 18.0 40.0 30 32.74013954796621 37.0 31.0 39.0 17.0 40.0 31 33.49956901635923 38.0 33.0 39.0 19.0 40.0 32 33.40135011296308 38.0 33.0 39.0 19.0 40.0 33 33.2001215827534 38.0 33.0 39.0 19.0 40.0 34 33.0677778483482 38.0 33.0 39.0 18.0 40.0 35 32.97618248301017 38.0 33.0 39.0 18.0 40.0 36 32.334316278478944 36.0 31.0 39.0 13.0 40.0 37 32.063722065455075 36.0 31.0 38.0 11.0 40.0 38 31.415168809487085 36.0 30.0 38.0 4.0 40.0 39 30.323092557139358 35.0 27.0 38.0 4.0 40.0 40 28.246459129140845 34.0 20.0 38.0 4.0 40.0 41 26.401431773021333 32.0 16.0 38.0 4.0 39.0 42 27.362470851895875 32.0 21.0 38.0 4.0 39.0 43 27.364131273080307 33.0 21.0 38.0 4.0 39.0 44 26.380426991371255 32.0 17.0 37.0 4.0 39.0 45 27.330015515411066 33.0 21.0 38.0 4.0 39.0 46 25.32796494061499 30.0 15.0 36.0 4.0 39.0 47 24.263662181412357 29.0 10.0 36.0 4.0 39.0 48 24.96638327602007 30.0 14.0 36.0 4.0 39.0 49 23.932131418253746 28.0 8.0 36.0 4.0 39.0 50 24.56447061598904 29.0 10.0 36.0 4.0 39.0 51 24.847549744585486 30.0 4.0 36.0 4.0 39.0 52 24.7030205148213 30.0 4.0 36.0 4.0 39.0 53 24.47948971536933 30.0 4.0 36.0 4.0 39.0 54 24.170714888443285 30.0 4.0 36.0 4.0 39.0 55 23.801693085207734 30.0 4.0 36.0 4.0 39.0 56 23.00220482157277 29.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.4808189015240956 1 2 3.2393993704407222 1 3 3.6177220391572575 1 4 5.037892862764416 1 5 3.0994358454358846 1 6 3.575695981110215 1 7 3.145913089514437 1 8 3.5468071382509763 1 9 3.222097415204175 1 10 4.303913990087196 1 11 -1.075359797507815 1 12 -7.526260661308697 1 13 -18.367518692346394 1 14 -7.036443041683913 1 15 0.5026248872766175 1 16 -2.0114186593856367 1 17 -6.526570168517036 1 18 -4.713171277826227 1 19 -0.13899937705051713 1 20 -1.3227090547051326 1 21 5.747836525734215 1 22 6.7764572765751865 1 23 5.7404883355531275 1 24 6.730967202492359 1 25 5.708820239673258 1 26 6.031027099097795 1 27 5.835813300542792 1 28 6.040056532652997 1 29 5.8757114461685624 1 30 6.243216390648275 1 31 4.411456395657012 1 32 4.932275617803672 1 33 4.719941215230801 1 34 4.29980082335474 1 35 4.3198200091347445 1 36 3.252086650384431 1 37 3.470330219004097 1 38 4.486374919460335 1 39 5.014196190572427 1 40 -9.068680870203554 1 41 0.5255981387676059 1 42 -0.6769968120879781 1 43 -8.455580012334298 1 44 -14.864743801445396 1 45 -1.960920555759504 1 46 -5.4035818369826245 1 47 -9.46823360698211 1 48 -4.25913113298407 1 49 -13.07763372692837 1 50 -3.203138581065364 1 51 2.8544583370422956 1 52 2.27831369063491 1 53 2.5788425568713116 1 54 2.4631146611539023 1 55 2.209427941358303 1 56 5.18419288883738 2 1 2.646427196289636 2 2 3.3867821422987916 2 3 3.7040421338725764 2 4 5.288135032606199 2 5 3.400479866531569 2 6 3.6210804033109127 2 7 3.23492396024956 2 8 3.019486792396563 2 9 3.2158906918518255 2 10 3.845872157489339 2 11 3.4806980362381026 2 12 -0.05171540245604689 2 13 0.9595720406209907 2 14 4.364159692144526 2 15 4.5152894730765 2 16 4.823941725215949 2 17 6.605923642698116 2 18 5.277823591760832 2 19 6.057427272395188 2 20 5.14469667844164 2 21 6.603216283923146 2 22 7.062971107163925 2 23 6.080100146266009 2 24 7.01529253525166 2 25 5.6984876597572125 2 26 5.4370472619794405 2 27 5.665002838805631 2 28 5.146611263037244 2 29 5.354454315612113 2 30 5.306862212770039 2 31 3.8647265854464123 2 32 4.044965317513071 2 33 3.987224966531347 2 34 3.1327574480453 2 35 4.789521871151749 2 36 4.897227108892668 2 37 4.901679553485501 2 38 4.972006175514583 2 39 5.493441338206338 2 40 4.628660643088882 2 41 8.371972766543585 2 42 7.330788499862653 2 43 6.642902389990532 2 44 -3.7515159465320416 2 45 6.652038221551873 2 46 6.323896367731617 2 47 3.099807149305338 2 48 5.748833564034548 2 49 -0.9459650870259573 2 50 6.037560303159513 2 51 8.266792854317078 2 52 7.425983478601758 2 53 7.6798363501479585 2 54 8.243009182733925 2 55 7.121852151167079 2 56 7.403042671781449 3 1 2.970972445231517 3 2 3.4421923067522826 3 3 3.7929245609347078 3 4 5.087786093961242 3 5 3.6274838405550227 3 6 3.6790752984677724 3 7 3.303354705621274 3 8 3.5004728671598997 3 9 3.6108375433267383 3 10 4.298490812783193 3 11 4.166039382236612 3 12 5.028055848876704 3 13 5.235233570135385 3 14 4.9228492336779155 3 15 0.8071701877545365 3 16 6.261536633296462 3 17 7.181866261676504 3 18 8.130946269541841 3 19 6.614178265901046 3 20 5.908419983019847 3 21 7.021910348735268 3 22 7.719751178890206 3 23 6.43201123152981 3 24 7.309111797957222 3 25 6.322215487614152 3 26 6.138575484007806 3 27 5.416093272071112 3 28 5.512415275831657 3 29 5.816022600966381 3 30 5.558065728003793 3 31 5.355631564283932 3 32 5.162150545777099 3 33 5.230092725246731 3 34 4.574552543518795 3 35 4.881796757262052 3 36 5.952184945473068 3 37 5.722880807079889 3 38 6.187185006570122 3 39 6.925188293070477 3 40 9.02829231896365 3 41 10.53329227156766 3 42 9.550878319254597 3 43 9.926415039016408 3 44 10.149424249261312 3 45 9.299222480541886 3 46 11.630380810883697 3 47 12.232339216120625 3 48 11.224650177391144 3 49 12.171866323913918 3 50 11.49773929576072 3 51 10.428467759812865 3 52 9.829783032735712 3 53 10.549048976557447 3 54 10.35021088865619 3 55 10.999788243700436 3 56 9.351057275509923 4 1 2.472124773566165 4 2 3.518277547478405 4 3 3.944242635125711 4 4 5.367533528846799 4 5 3.372108939213277 4 6 3.9136451485072357 4 7 3.302091880049055 4 8 3.7167633170415115 4 9 3.5644287035477333 4 10 4.103984104022338 4 11 4.708233541668342 4 12 5.1950013895239024 4 13 5.4747600105458005 4 14 5.545011822470336 4 15 5.775473265891868 4 16 7.298695275758977 4 17 8.144770760492605 4 18 8.962201202454228 4 19 7.277477397708466 4 20 6.72808849130714 4 21 6.92157885702256 4 22 7.8535475482666826 4 23 6.363976503826574 4 24 7.430564047365273 4 25 6.613675629682028 4 26 6.097565223550031 4 27 5.731168252339462 4 28 5.716235323187618 4 29 6.120268851952968 4 30 6.2222172670724625 4 31 5.184392416691189 4 32 5.00316080623487 4 33 4.848845684994171 4 34 4.9806772475440475 4 35 5.225411595462525 4 36 5.34624966528364 4 37 5.242565100686832 4 38 5.438171589048412 4 39 5.819489792675846 4 40 0.9391368335650725 4 41 9.591855807479263 4 42 8.177271373713946 4 43 8.668767051644668 4 44 9.518358740184755 4 45 8.546073309271172 4 46 10.985550503069963 4 47 11.481494701519207 4 48 11.67254283721751 4 49 11.035354879557168 4 50 11.52394292638424 4 51 10.95310864379076 4 52 10.42618397985489 4 53 10.769727745302514 4 54 11.15478863135548 4 55 11.175352568878026 4 56 9.499249856409687 5 1 3.0267937631132042 5 2 3.7738524603703922 5 3 3.9079360149166504 5 4 5.950843633376415 5 5 3.829983503673205 5 6 3.725666054430583 5 7 3.521081427087381 5 8 3.641188404149524 5 9 3.8668677279379793 5 10 4.540708842698301 5 11 5.0146446914941265 5 12 5.5775101003252985 5 13 5.492948163855907 5 14 5.416684296337934 5 15 6.331257238017301 5 16 7.356604683424482 5 17 8.168289924255667 5 18 8.390075766914162 5 19 7.489498303631812 5 20 6.838820198624212 5 21 7.324261783851824 5 22 7.815324551751004 5 23 6.792373716370125 5 24 7.244396799978517 5 25 6.847717441528715 5 26 6.06031783679045 5 27 6.267056754081626 5 28 5.299371211689362 5 29 6.25518174393903 5 30 6.018279984842497 5 31 5.423277434112791 5 32 5.642847217384702 5 33 5.597765545621346 5 34 5.473151115139867 5 35 5.3718924316994645 5 36 5.663915170510126 5 37 6.3520076093976385 5 38 6.878938139571066 5 39 8.166597806613126 5 40 8.8278476349588 5 41 11.577221661137798 5 42 10.791243499149491 5 43 11.024481337358953 5 44 11.512818670498344 5 45 10.424609894637022 5 46 12.65541113024766 5 47 13.347418046466938 5 48 13.112333777984059 5 49 13.73678345098574 5 50 12.268019581436509 5 51 11.384572058424911 5 52 12.12377979867022 5 53 11.460145863769409 5 54 12.066286889195197 5 55 11.175910060167219 5 56 10.05113138951073 6 1 2.7585010801863703 6 2 3.7738524603703922 6 3 3.877448210038601 6 4 5.609380218742267 6 5 3.6165688695268656 6 6 4.030544103211071 6 7 3.350349719770314 6 8 3.62289572122269 6 9 3.3668677279379793 6 10 3.8090015256251277 6 11 4.709766642713639 6 12 5.3701930271545635 6 13 5.4014847492217655 6 14 5.014245271947686 6 15 6.178818213627061 6 16 6.771238829765945 6 17 8.070728948645915 6 18 8.603490401060501 6 19 7.245595864607424 6 20 7.161990930331527 6 21 6.964505686290849 6 22 7.101909917604665 6 23 6.274081033443299 6 24 7.238299239002906 6 25 6.122107685431153 6 26 5.542025153863623 6 27 5.114617729691378 6 28 4.866444382421065 6 29 5.50518174393903 6 30 5.524377545818108 6 31 4.179374995088402 6 32 4.636749656409091 6 33 4.927033838304272 6 34 4.009736480993528 6 35 4.792624139016532 6 36 5.493183463193052 6 37 5.766641755739101 6 38 5.647230822497892 6 39 6.422695367588737 6 40 4.120530561788069 6 41 10.278441173332922 6 42 9.029048377198269 6 43 9.57935938613944 6 44 9.683550377815411 6 45 9.455097699515072 6 46 11.40541113024766 6 47 11.993759509881574 6 48 11.892821582862108 6 49 11.474588329034518 6 50 10.755824459485286 6 51 10.555303765741986 6 52 10.459145652328758 6 53 10.612584888159656 6 54 10.139457620902519 6 55 9.621032011386735 6 56 10.563326511461952 7 1 2.922656986451315 7 2 3.8766342234821423 7 3 3.9905918414749166 7 4 5.670315975158658 7 5 3.5563106198855436 7 6 4.026997155976893 7 7 3.2290760070331856 7 8 3.692001412279602 7 9 3.6833829519137424 7 10 4.1382698183080535 7 11 4.567729339987672 7 12 5.263386076732118 7 13 5.5595032411713845 7 14 5.113041701088449 7 15 6.1238206048274435 7 16 7.096043548401365 7 17 7.731138640978131 7 18 8.591893079204933 7 19 6.666646398642172 7 20 6.710572151437859 7 21 6.837134412578116 7 22 7.392520470769021 7 23 6.94903719476962 7 24 6.907277399372745 7 25 6.344170494294538 7 26 5.5536623290588985 7 27 5.3636612495383424 7 28 5.260474352132526 7 29 5.853938319740077 7 30 5.02684845288012 7 31 4.075596898483909 7 32 4.335298994843651 7 33 4.532804601894259 7 34 4.984150636899795 7 35 4.578651558114252 7 36 5.942290748383552 7 37 5.771304596485152 7 38 6.402690730038142 7 39 7.008061221247274 7 40 7.419510316291493 7 41 10.122574761727627 7 42 9.297420766804517 7 43 9.640494409254377 7 44 9.826743427392966 7 45 9.324896838682939 7 46 11.441199429307122 7 47 12.965304225328726 7 48 11.419284200429455 7 49 12.748221359482471 7 50 11.498172618260991 7 51 11.302912565359389 7 52 10.666781552217167 7 53 10.452454169205406 7 54 10.736301236397495 7 55 10.67327973974637 7 56 10.041845561358333 8 1 2.8813022627436524 8 2 3.6957667249675836 8 3 3.7340261849092613 8 4 5.810304091617361 8 5 3.8789487660530995 8 6 4.04351527837737 8 7 3.1644295423867206 8 8 3.5526074728856614 8 9 3.764428703547729 8 10 4.532209212247444 8 11 4.812649126083926 8 12 5.71032606484858 8 13 5.930863906649698 8 14 5.373410090868603 8 15 5.895300105718711 8 16 7.447439864503561 8 17 7.92745474317659 8 18 8.110253150506178 8 19 7.4618929821240485 8 20 6.580209703428352 8 21 6.960366735810439 8 22 7.968872223591362 8 23 7.146660486510559 8 24 7.230390887192115 8 25 6.80917346517986 8 26 5.731677777662586 8 27 5.880951802123011 8 28 5.591906318858612 8 29 5.939143310827422 8 30 6.6263731112283075 8 31 5.465258217556993 8 32 5.211472494546559 8 33 5.1558586720071595 8 34 4.892885039751842 8 35 4.890000340051273 8 36 6.028327587361563 8 37 6.568452546574278 8 38 6.375833926710751 8 39 7.6444248576109075 8 40 4.240781855210091 8 41 10.0342800499035 8 42 9.77502029146287 8 43 9.862360125237739 8 44 10.83731977914579 8 45 9.581224824422684 8 46 12.204598122117577 8 47 11.535001195025696 8 48 11.752023356698029 8 49 12.547389511591803 8 50 11.642557644998963 8 51 11.653714704396823 8 52 11.231725105396016 8 53 11.155009130583895 8 54 11.08639036295721 8 55 11.179162092687548 8 56 10.616652453812282 9 1 2.9878014652954903 9 2 3.4752324346964514 9 3 3.864129853170823 9 4 5.0776087168167265 9 5 3.225041270040343 9 6 3.887893268807993 9 7 3.4037836093723683 9 8 3.5779663245603075 9 9 3.438112914074047 9 10 4.283006660413314 9 11 4.672218504074358 9 12 5.419775825614131 9 13 5.2161509879894155 9 14 4.947493025477854 9 15 5.174646198222696 9 16 7.245725350946945 9 17 7.844958730417417 9 18 8.258817743807615 9 19 7.362251833798695 9 20 6.727299017622926 9 21 6.656578857022559 9 22 7.795427247514802 9 23 7.026006579014545 9 24 7.354473821801363 9 25 6.895856080809843 9 26 6.308392291219203 9 27 5.770265996700363 9 28 5.692663894616189 9 29 6.082524491050709 9 30 6.452330049027349 9 31 5.225106702405476 9 32 4.794965317513071 9 33 4.775236662437781 9 34 5.367406570852321 9 35 5.61627625711666 9 36 5.772227108892668 9 37 5.868053822491348 9 38 6.689111438672473 9 39 7.218587537036747 9 40 3.9554150290537926 9 41 9.505013702216104 9 42 9.522308967698908 9 43 10.202112916306323 9 44 10.469975281538137 9 45 9.891073309271171 9 46 11.843633209836877 9 47 12.509163874451534 9 48 12.50979847631525 9 49 12.618362398354158 9 50 12.34311585871507 9 51 11.944424433264448 9 52 12.07949225053158 9 53 11.652058572370183 9 54 12.067570586242695 9 55 11.975653320757722 9 56 11.131843841372092 10 1 2.778866136778113 10 2 3.6282682104102477 10 3 4.055951318599099 10 4 5.330446694112908 10 5 3.7654609466829285 10 6 3.942029835715452 10 7 3.5035858109547533 10 8 3.600498144305746 10 9 3.4546247819791063 10 10 4.007550864059688 10 11 4.842239143909239 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0.862532935751581 114 21 5.4297270051707045 114 22 5.798838553557687 114 23 4.694135233985303 114 24 5.359347116148342 114 25 5.099072455078854 114 26 5.010634006618812 114 27 4.281961903133112 114 28 4.095441672393967 114 29 4.68291435459847 114 30 5.001793986649179 114 31 3.9612178135165905 114 32 3.7345364676105355 114 33 3.9331313992798833 114 34 3.794063490930295 114 35 3.507845457896387 114 36 4.551223210257188 114 37 5.046903725025459 114 38 5.505631906508729 114 39 6.526579739765793 114 40 8.054586569014809 114 41 8.76037432599778 114 42 7.892192008634584 114 43 8.302259115136732 114 44 -0.09264655082053963 114 45 7.77533256853043 114 46 1.7527462702657282 114 47 1.2708597808842832 114 48 2.503706858381527 114 49 -5.679883215680926 114 50 2.6130963655376824 114 51 5.320886421568542 114 52 5.601422075092987 114 53 5.0662885918633584 114 54 6.272248948815797 114 55 6.610643574169028 114 56 7.7324773696371984 115 1 2.441748833716538 115 2 3.1265482241701363 115 3 3.239129853170823 115 4 5.261819243132514 115 5 3.075918463022795 115 6 3.2146476547729037 115 7 2.9542222058635943 115 8 2.9551593070164444 115 9 2.7188146684600127 115 10 4.024234730588752 115 11 1.755551837407694 115 12 -0.4376803147367454 115 13 -0.38253322253690314 115 14 -3.6446122376800396 115 15 -3.6104415210755505 115 16 3.186514824631157 115 17 4.246274519891102 115 18 4.614080901702348 115 19 3.925848325026763 115 20 3.273351649201878 115 21 6.228947278075189 115 22 6.948936019444631 115 23 6.144427631646121 115 24 6.521140488468035 115 25 5.944101694844932 115 26 5.089094045605169 115 27 4.472020382665274 115 28 4.9711726665460105 115 29 4.7404192278928114 115 30 5.388733557799281 115 31 3.306247053282668 115 32 3.4923337385657014 115 33 3.7094471887535683 115 34 4.281880255062848 115 35 3.780749941327187 115 36 4.5902095650330175 115 37 4.240860840035204 115 38 4.910602666742651 115 39 6.271219115984117 115 40 7.391818537825721 115 41 7.798873351338912 115 42 6.588098441383121 115 43 5.862200635604566 115 44 -1.4971299816197607 115 45 5.818704888218541 115 46 2.1747735607140726 115 47 -7.359257178180043 115 48 0.7751493535082332 115 49 -2.0833919876107494 115 50 1.9703088411712102 115 51 3.3632840823872527 115 52 3.496158917198251 115 53 3.393286642545622 115 54 3.3395004108040993 115 55 3.58968840847702 115 56 5.563861385231739 116 1 2.880071202137593 116 2 3.725643618906979 116 3 3.775314063697138 116 4 5.964944243132514 116 5 3.7295642963561306 116 6 3.7957880056500954 116 7 3.4391454514776285 116 8 3.6659597456129376 116 9 3.628491203547732 116 10 4.3413948183080535 116 11 3.8572513988112007 116 12 3.5077692466667614 116 13 4.428733224831518 116 14 4.299877893898909 116 15 -3.132919591250989 116 16 4.43034706147326 116 17 5.88361004620689 116 18 5.922469059597088 116 19 5.137745254851325 116 20 4.879025991307138 116 21 6.305016357022559 116 22 7.0413154054095415 116 23 6.46844078954086 116 24 6.995921190222418 116 25 6.26921134396774 116 26 5.662833080692888 116 27 5.614427180910891 116 28 5.341101394616189 116 29 5.577590280524394 116 30 5.728645838501034 116 31 4.803231702405476 116 32 4.653517949092016 116 33 4.511256399279883 116 34 4.557373676115478 116 35 4.320634809748242 116 36 5.987276450997928 116 37 6.323234743543978 116 38 6.515180517619843 116 39 7.914311221247274 116 40 9.696368976422214 116 41 9.710605807479261 116 42 6.598789230856806 116 43 3.735418837358953 116 44 3.110189097327609 116 45 5.805135809271171 116 46 0.7322022887842472 116 47 -6.552924941337938 116 48 0.6611142657889353 116 49 -2.2397790490142597 116 50 2.394925069241385 116 51 5.464983643790763 116 52 5.419130408426319 116 53 5.854772388159656 116 54 5.203672559926908 116 55 5.10312042602088 116 56 7.119919499266828 117 1 1.676610180632217 117 2 2.577878161917731 117 3 2.664426966922946 117 4 5.10590526463789 117 5 2.4791442694744106 117 6 2.783691231456544 117 7 2.0498645374991327 117 8 2.380173455290354 117 9 2.0719555852681637 117 10 3.267302076372573 117 11 0.4058065063380809 117 12 -9.131511667311734 117 13 -8.240201586996438 117 14 -1.0353875630903389 117 15 -7.612422279423033 117 16 -7.053439900892332 117 17 1.021882895669254 117 18 -2.2430215318007605 117 19 1.1174159537760566 117 20 0.479916448296386 117 21 5.313836921538687 117 22 5.828109760248253 117 23 5.013131649755916 117 24 5.374281405276179 117 25 4.885810806333332 117 26 4.684170446284288 117 27 5.011352584136699 117 28 4.470889701067804 117 29 4.581958560094289 117 30 5.332140462156946 117 31 3.823224981975372 117 32 3.423007196403841 117 33 3.672378711107843 117 34 4.621721794395047 117 35 4.558622040931034 117 36 4.953254273578576 117 37 5.205207189780538 117 38 5.393624738049947 117 39 6.965050468559099 117 40 8.488875696852318 117 41 7.776802044038401 117 42 6.254199177093366 117 43 -3.123368125006639 117 44 0.2275446080802972 117 45 6.061772234002355 117 46 -1.1158319854093008 117 47 -1.5078140542411624 117 48 0.005200287294311323 117 49 -0.7820337533153356 117 50 1.6044344778435367 117 51 6.922140901855279 117 52 5.9240027202542755 117 53 6.05882144729944 117 54 7.64142457605594 117 55 6.508194350752063 117 56 8.211569364858224 118 1 2.119461634236359 118 2 2.7045209337217955 118 3 3.114048631598372 118 4 4.490214304860906 118 5 2.6006098210474917 118 6 2.849800351329108 118 7 2.648829093452939 118 8 2.8771865974647923 118 9 2.79158919737489 118 10 3.3851833985549646 118 11 -0.6356112555097866 118 12 -8.495510074320894 118 13 -6.28799131220552 118 14 -7.4149176307924485 118 15 -7.552286875201606 118 16 -9.816084265687234 118 17 -1.3102556945338506 118 18 -5.175717668797976 118 19 -1.3369846834202797 118 20 -0.7701831136311341 118 21 4.454418363195401 118 22 4.8050113930638645 118 23 4.2867278265778985 118 24 5.0074952642964945 118 25 4.586726776066506 118 26 3.908782154766964 118 27 4.436282890787435 118 28 4.246676240295201 118 29 4.577976082993526 118 30 5.42771991257511 118 31 4.177267196232634 118 32 4.083301899709301 118 33 4.411526461008275 118 34 4.087273367473504 118 35 3.8720429887605903 118 36 4.767272592973239 118 37 4.423446934902003 118 38 5.977854128730957 118 39 6.7734933200127045 118 40 8.270635951730853 118 41 7.732596548220002 118 42 7.287253737029648 118 43 -0.21934582313487283 118 44 0.6110571528831663 118 45 6.846320222851418 118 46 1.3206475048336301 118 47 -0.02235009565892554 118 48 -0.227774623099954 118 49 -0.88358691938463 118 50 2.0297630322043467 118 51 6.1881703721858266 118 52 5.984138124475702 118 53 5.149621925196694 118 54 6.06237240560592 118 55 5.910026290218411 118 56 8.4361810733409 119 1 1.9806962021375938 119 2 2.7597061189069763 119 3 3.0128140636971352 119 4 4.801819243132513 119 5 2.6592517963561306 119 6 2.9707880056500926 119 7 2.4735204514776257 119 8 2.895334745612935 119 9 2.6544287035477296 119 10 3.6082698183080524 119 11 0.505376398811201 119 12 -6.26785575333324 119 13 -2.8609542751684813 119 14 -4.33355960610109 119 15 -6.36166959125099 119 16 -9.935590438526742 119 17 0.4904850462068886 119 18 -6.390655940402912 119 19 0.10462025485132642 119 20 -0.611911508692863 119 21 4.371578857022563 119 22 5.020690405409539 119 23 4.469690789540859 119 24 3.6834211902224183 119 25 4.707961343967739 119 26 4.934708080692889 119 27 5.339739680910892 119 28 4.877663894616191 119 29 5.508840280524392 119 30 5.623645838501037 119 31 4.340106702405478 119 32 4.4760179490920144 119 33 4.66313139927988 119 34 4.189248676115479 119 35 4.279697309748244 119 36 4.411963950997926 119 37 5.205422243543978 119 38 4.9967430176198455 119 39 6.8080612212472715 119 40 8.291993976422212 119 41 6.861855807479262 119 42 5.354414230856804 119 43 -2.685518662641048 119 44 -0.8644984026723925 119 45 5.746073309271171 119 46 0.5712647887842479 119 47 -2.21136244133794 119 48 -3.948885734211064 119 49 -1.3232165490142584 119 50 1.636800069241385 119 51 6.633108643790763 119 52 6.80475540842632 119 53 6.792584888159656 119 54 6.193360059926906 119 55 6.982495426020879 119 56 9.73210699926683 120 1 1.6696323723503568 120 2 2.2939614380559163 120 3 2.2660055530588394 120 4 4.793734136749535 120 5 2.2177624346540057 120 6 2.606958218416054 120 7 2.1735204514776285 120 8 2.652994320081021 120 9 2.6133648737604958 120 10 3.148908116180394 120 11 -1.6018576437419902 120 12 -9.901472774609836 120 13 -8.70840108367912 120 14 -9.500155350781942 120 15 -1.3144355486977979 120 16 -10.730058523633122 120 17 -1.3461106984739608 120 18 -9.078528280828444 120 19 -0.767507404723144 120 20 -0.7680817214588203 120 21 3.2496639634055384 120 22 4.618775511792521 120 23 4.387988661881284 120 24 4.241931828520293 120 25 4.413493258861358 120 26 4.90917616579927 120 27 4.698037553251314 120 28 4.259153256318314 120 29 4.991819003928651 120 30 5.07705009382018 120 31 4.49904287261824 120 32 4.168145608666482 120 33 4.598025016301161 120 34 4.5377593144133535 120 35 3.561824969322707 120 36 4.823878844614949 120 37 4.884571179714193 120 38 4.909296209109204 120 39 5.912316540396212 120 40 5.557738657273276 120 41 -7.125165469116482 120 42 -4.226011301058087 120 43 -8.353178237109134 120 44 -15.63598776437452 120 45 -4.893501158813937 120 46 -8.90958627504554 120 47 -8.307745420061343 120 48 -5.8708006278280855 120 49 -14.481301655397239 120 50 -4.592774398843723 120 51 -0.08987007961349391 120 52 -0.5333296979566597 120 53 -0.24911723949991682 120 54 0.24506218758648046 120 55 0.13802734091449764 120 56 2.223809126926401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2248.0 5 104.0 6 348.0 7 294.0 8 496.0 9 566.0 10 524.0 11 325.0 12 295.0 13 281.0 14 321.0 15 429.0 16 525.0 17 592.0 18 762.0 19 929.0 20 1252.0 21 1669.0 22 2096.0 23 2548.0 24 2743.0 25 3190.0 26 3417.0 27 3652.0 28 3821.0 29 3991.0 30 4589.0 31 4954.0 32 5525.0 33 6342.0 34 7170.0 35 7649.0 36 8317.0 37 9193.0 38 9706.0 39 9347.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 98.93841924273906 0.7322185223158792 0.11432408155117818 0.21503815339388274 8 99.11353470098808 0.5353270485332946 0.10978741164835364 0.24135083883026504 9 96.24813994846296 1.7929082132617138 1.2140238812470512 0.7449279570282727 10 52.393093373739944 27.173745383938375 12.50941359004836 7.923747652273325 11 34.83527351582844 26.397067496574817 19.922332211263644 18.845326776333103 12 29.6525818188417 18.62847395497809 21.86311959569198 29.855824630488236 13 23.468193407311297 16.3011622948291 25.067823215047223 35.16282108281237 14 22.027347046174224 24.090624517978824 28.904938618856214 24.977089816990734 15 30.336463937266345 20.0246193124829 23.96462113613568 25.674295614115074 16 32.00871578373962 24.19628671296927 22.3614326569522 21.433564846338918 17 27.775004764540924 21.088311900461935 29.662670502500248 21.474012832496893 18 25.098672570386434 20.683585420957602 26.99590792374765 27.221834084908313 19 29.01287241961546 22.86170772889849 22.019281395813287 26.106138455672763 20 26.979743845944228 25.724407303115633 25.483695158506674 21.812153692433466 21 26.269371756251587 21.066671505825138 27.444379922331507 25.219576815591772 22 23.99444711603894 20.379628537468356 27.874207216934483 27.751717129558216 23 23.000009073339804 22.214257846170597 26.464210211136617 28.32152286935298 24 23.160340118152035 22.41531076163598 27.567901122535098 26.856447997676884 25 28.627689403907407 24.069654540339926 24.080543733722926 23.222112322029744 26 28.364468166221883 23.36746364577093 21.77078227402284 26.497285913984353 27 23.36250885253046 24.75530697851786 21.769053369409285 30.113130799542393 28 25.1088850174216 27.746624564459932 22.31870644599303 24.825783972125436 29 31.334684344925982 24.479966557919322 20.272443907270926 23.91290518988377 30 27.232284715602162 22.130827545508193 27.778836987607246 22.8580507512824 31 28.718953892503563 26.819662610368518 21.982958103068086 22.478425394059837 32 32.41123938139839 23.619581790459595 21.771763595440355 22.197415232701662 33 28.41169203938524 23.761944555462705 21.88714892998583 25.93921447516622 34 24.986365123804678 26.058066392757155 27.427916954514053 21.527651528924117 35 24.202635165833712 25.090413448432532 29.953657428441616 20.75329395729214 36 26.401031311563216 27.69107861027135 24.086935207124764 21.820954871040662 37 27.236632898741654 23.34529596147731 27.384727206650616 22.03334393313042 38 29.708794355901848 25.153876226236694 23.076433526379432 22.06089589148203 39 26.876669513546908 23.443150224418964 22.436444912867294 27.24373534916683 40 22.86592618346173 18.672802796025543 25.12488792109645 33.336383099416274 41 19.627007995305508 17.025783026479864 22.924154624807453 40.423054353407174 42 25.93933659637184 24.50645026292327 24.055204789024547 25.49900835168034 43 21.194999454088876 20.769188776067256 28.63212868944936 29.40368308039451 44 22.108967579592285 22.72893038182415 24.698223988913607 30.46387804966996 45 25.04364112448631 22.531548896243226 28.604029530494234 23.82078044877623 46 22.608101957214384 25.658625398270367 24.26763768775603 27.465634956759217 47 19.778916838437823 24.462341073676146 24.33282866054979 31.425913427336237 48 24.69583060942834 25.270574562359265 25.77358901721508 24.260005810997313 49 22.569580289148295 25.375537945027247 23.842451482590143 28.212430283234312 50 25.73163194823136 23.972079834224015 25.27811102628422 25.018177191260406 51 22.228288066966925 26.74036668031482 27.173467995086664 23.85787725763159 52 22.60615093790897 25.182674131161843 28.738912316417043 23.472262614512143 53 22.56911279098327 24.493081898316184 29.12516260495411 23.812642705746438 54 23.072445313565872 25.21078721178771 27.655646004820593 24.06112146982582 55 22.003366237547194 25.152163035072554 27.171905563389892 25.672565163990356 56 25.009094216078577 22.78010185522008 28.31211349581666 23.89869043288469 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 19.5 9 37.0 10 85.0 11 133.0 12 167.0 13 194.0 14 187.0 15 188.5 16 190.0 17 186.0 18 182.0 19 191.0 20 200.0 21 205.5 22 228.0 23 245.0 24 277.5 25 310.0 26 357.5 27 405.0 28 461.0 29 592.5 30 668.0 31 777.0 32 886.0 33 990.0 34 1094.0 35 1264.5 36 1435.0 37 1585.5 38 1867.5 39 1999.0 40 2239.0 41 2479.0 42 2804.5 43 3130.0 44 3754.0 45 4378.0 46 6064.5 47 7481.0 48 7211.0 49 6487.0 50 5763.0 51 5985.5 52 6208.0 53 6409.5 54 6836.5 55 7062.0 56 6900.0 57 6738.0 58 6995.5 59 7253.0 60 7519.5 61 7786.0 62 7473.0 63 6340.5 64 5521.0 65 5068.5 66 4616.0 67 3875.5 68 3135.0 69 2722.0 70 2309.0 71 1946.5 72 1369.0 73 1154.0 74 926.5 75 699.0 76 552.0 77 405.0 78 331.0 79 267.5 80 278.0 81 173.0 82 68.0 83 42.5 84 17.0 85 8.5 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 9.073339805649063E-4 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.49268235144674405 16 0.061698710678413614 17 0.021776015533557746 18 0.0 19 0.049903368931069836 20 0.11160207960948344 21 9.073339805649063E-4 22 0.0 23 0.0 24 0.0145173436890385 25 0.010888007766778873 26 0.04264469708655059 27 0.06805004854236796 28 0.0045366699028245305 29 0.15606144465716384 30 0.061698710678413614 31 0.012702675727908685 32 0.026312685436382276 33 0.10978741164835364 34 0.18237413009354614 35 0.1478954388320797 36 0.05625470679502419 37 0.1342854291236061 38 0.20142814368540918 39 0.13700743106530083 40 0.8302105922168891 41 1.0425267436690773 42 0.2676635242666473 43 0.2767368640722964 44 0.4799796757188353 45 0.2041501456271039 46 0.32936223494506095 47 0.5180877029025613 48 0.06986471650349776 49 0.27582953009173145 50 0.16785678640450763 51 0.27945886601399106 52 0.16422745048224802 53 0.2576828504804334 54 0.24316550679139484 55 0.27038552620834205 56 0.22955549708292125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 110213.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.68731456361772 #Duplication Level Percentage of deduplicated Percentage of total 1 88.76288278970115 65.40698465698239 2 6.945932301478827 10.23654196873327 3 1.8199056801251032 4.023118869824794 4 0.821297082979326 2.4207670601471696 5 0.4100328765099184 1.5107110776405688 6 0.267198601209166 1.1813488426955077 7 0.19578146355878984 1.0098627203687407 8 0.13544629554381687 0.7984539028971174 9 0.10096905667811804 0.6696124776569007 >10 0.49376331375518695 6.853093555206737 >50 0.02585792914927413 1.3283369475470226 >100 0.018469949392338664 2.5868091785905474 >500 0.0012313299594892443 0.4663696660103618 >1k 0.0012313299594892443 1.5079890756988739 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1662 1.5079890756988739 No Hit TGGATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 514 0.4663696660103618 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 386 0.35023091649805377 No Hit TGGATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 380 0.3447869126146643 No Hit TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 273 0.24770217669421937 No Hit TGGATGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 245 0.222296825238402 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 205 0.18600346601580575 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 189 0.17148612232676727 No Hit TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 167 0.15152477475433931 No Hit TGGATGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 152 0.13791476504586572 No Hit TGGATGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 136 0.12339742135682723 No Hit TGGATGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 135 0.12249008737626234 No Hit TGGATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 127 0.11523141553174308 No Hit TGGATGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 121 0.10978741164835364 No Hit TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 115 0.10434340776496422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.073339805649061E-4 0.0 0.0 0.0 0.0 8 9.073339805649061E-4 0.0 0.0 0.0 0.0 9 0.0018146679611298123 0.0 0.0 0.0 0.0 10 0.006351337863954343 0.0 0.0 0.0 0.0 11 0.010888007766778873 0.0 0.0 0.0 0.0 12 0.010888007766778873 0.0 0.0 0.0 0.0 13 0.015424677669603403 0.0 0.0 0.0 0.0 14 0.027220019416947183 0.0 0.0 0.0 0.0 15 0.031756689319771714 0.0 0.0 0.0 0.0 16 0.03357135728090153 0.0 0.0 0.0 0.0 17 0.03901536116429096 0.0 0.0 0.0 0.0 18 0.045366699028245305 0.0 0.0 0.0 0.0 19 0.04808870096994002 0.0 0.0 0.0 0.0 20 0.05534737281445928 0.0 0.0 0.0 0.0 21 0.05716204077558908 0.0 0.0 0.0 0.0 22 0.0589767087367189 0.0 0.0 0.0 0.0 23 0.06260604465897852 0.0 0.0 0.0 0.0 24 0.06351337863954343 0.0 0.0 0.0 0.0 25 0.06623538058123815 0.0 0.0 0.0 0.0 26 0.07077205048406268 0.0 0.0 0.0 0.0 27 0.07621605436745212 0.0 0.0 0.0 0.0 28 0.07712338834801702 0.0 0.0 0.0 0.0 29 0.07712338834801702 0.0 0.0 0.0 0.0 30 0.07712338834801702 0.0 0.0 0.0 0.0 31 0.08256739223140645 0.0 0.0 0.0 0.0 32 0.08528939417310118 0.0 0.0 0.0 0.0 33 0.08619672815366608 0.0 0.0 0.0 0.0 34 0.08710406213423098 0.0 0.0 0.0 0.0 35 0.09164073203705551 0.0 0.0 0.0 0.0 36 0.09345539999818533 0.0 0.0 0.0 0.0 37 0.09527006795931514 0.0 0.0 0.0 0.0 38 0.09617740193988004 0.0 0.0 0.0 0.0 39 0.09708473592044496 0.0 0.0 0.0 0.0 40 0.09889940388157477 0.0 0.0 0.0 9.073339805649061E-4 41 0.10162140582326949 0.0 0.0 0.0 9.073339805649061E-4 42 0.1025287398038344 0.0 0.0 0.0 9.073339805649061E-4 43 0.10888007766778873 0.0 0.0 0.0 9.073339805649061E-4 44 0.10978741164835364 0.0 0.0 0.0 9.073339805649061E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTATA 15 0.008071681 50.523018 41 CGCATTG 20 3.9946323E-4 50.43015 39 GCGCATT 20 3.9946323E-4 50.43015 38 ATATCAT 20 4.0037066E-4 50.406982 37 ACGCTTA 15 0.0081454255 50.406982 37 AATATCA 15 0.0082196705 50.291473 36 TCAGTCA 15 0.0082196705 50.291473 36 TAAGACC 15 0.008234579 50.26844 35 TTATACT 20 4.0861152E-4 50.199455 43 ATTATAC 20 4.0861152E-4 50.199455 42 TATACTA 20 4.0861152E-4 50.199455 44 TAATCCG 15 0.008279428 50.19945 42 GTTACAG 15 0.008309427 50.153564 34 CAAACAT 15 0.0083395075 50.107765 14 TCGTGAT 20 4.1324768E-4 50.084896 12 ACTAATC 20 4.1324768E-4 50.084896 10 GACCCAC 15 0.008369668 50.062046 9 GACGGAA 15 0.008369668 50.062046 9 CGATTAA 40 2.6157068E-9 50.062042 49 GACTAAT 20 4.141799E-4 50.062042 9 >>END_MODULE