FastQCFastQC Report
Fri 17 Jun 2016
SRR1526069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11288
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1030.9124734231041814No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC570.5049610205527995No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG520.4606661941885188No Hit
TGGATGGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT510.4518072289156626No Hit
TGGATGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC430.3809355067328136No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG400.3543586109142452No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT390.3454996456413891No Hit
TGGATGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT320.28348688873139616No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG320.28348688873139616No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC310.27462792345854004No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC290.2569099929128278No Hit
TGGATGGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC200.1771793054571226No Hit
TGGATGGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT180.15946137491141033No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG170.15060240963855423No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC170.15060240963855423No Hit
TGGATGGGGGTGTGGCTTTGGCCCTAAGCCATTGTCTCTC140.12402551381998582No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC140.12402551381998582No Hit
TGGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA130.1151665485471297No Hit
TGGATGGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT120.10630758327427356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA650.034.2477522
TGCCAGT650.034.2477524
CTGCCAG650.034.2477523
GCGATGG200.002578764734.24774618
CCAGTAG601.8189894E-1234.24774626
GCCAGTA601.8189894E-1234.24774625
CGATGGC200.002578764734.24774619
AGTAGCA552.5465852E-1133.94196728
GTAGCAT650.033.94196729
CAGTAGC601.8189894E-1233.94196327
TAGCATA601.8189894E-1233.94196330
CGTGGTA251.92242E-433.94196329
GATGGGA351.0175027E-633.791113
ATGGGGG4950.033.791114
ATGGGGC750.033.791114
ATGGGAG200.002755840333.791114
GAGTGCA200.002755840333.7911110
TGGGGTT200.002755840333.791115
TGGGGTG407.312701E-833.791115
TGGGGTA1200.033.791115
Produced by FastQC (version 0.11.5)