FastQCFastQC Report
Fri 17 Jun 2016
SRR1526067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8483
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1441.6975126724036307No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC810.9548508782270423No Hit
TGGATGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT470.5540492750206295No Hit
TGGATGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG450.5304727101261346No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG370.4361664505481551No Hit
TGGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.4008016032064128No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTA170.2004008016032064No Hit
TGGATGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT120.14145938936696922No Hit
TGGATGGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT100.11788282447247435No Hit
TGGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.11788282447247435No Hit
TGGATGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT90.10609454202522692No Hit
TGGATGGGTGTTTTTTAAATTAAAGTTTCTGAAATCTAAA90.10609454202522692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTGG251.7734255E-434.28143326
TGGGGTA454.5274646E-934.0773855
ATGGGGT1300.034.077384
GGGTCAC200.00260585334.077387
GTCACAG200.00260585334.077389
AGTGGGT251.8377346E-434.0773828
GGTCACA200.00260585334.077388
GATTGGG200.00260585334.0773832
TGGGGGT800.034.077385
TGGGGGC359.140913E-734.077385
TGGGGGA650.034.077385
TGGGGCC200.00260585334.077385
TGGGGAT750.034.077385
GTGGGTA251.8377346E-434.0773829
CTCAGTG251.8377346E-434.0773825
TGGGTAA251.8377346E-434.0773830
GGGGTCA406.438495E-834.077386
GGGGTAT200.00260585334.077386
GCTCAGT251.8377346E-434.0773824
ATGGGGC601.8189894E-1234.0773774
Produced by FastQC (version 0.11.5)